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Citations for Lonza :
1 - 50 of 79 citations for Recombinant Monkey IL10 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: Embryonic monkey kidney cells (MA104) were grown in M199 complete medium [Medium 199 (Lonza) and 1% penicillin-streptomycin (P/S ...
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bioRxiv - Molecular Biology 2023Quote: Vero cells (African green monkey kidney cells, ATCC CCL-81) were grown in Dulbecco’s modified Eagle’s medium (DMEM, Lonza) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: ... and recombinant human insulin 0.5%) (Lonza). Human cervical cancer cell line SiHa from ATCC (ATCC® HTB-35™ ...
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bioRxiv - Biochemistry 2023Quote: ... Sf21 cells seeded at 500,000 cells/mL were transfected with recombinant bacmid DNA to generate the initial baculovirus (P1) in Insect-XPRESSTM Protein-free Insect Cell Medium (Lonza). The P1 virus was amplified two times (P2 and P3 ...
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bioRxiv - Microbiology 2021Quote: Vero E6 cells (C1008; green African monkey kidney cells) were obtained from Public Health England and cultured in DMEM medium (Lonza) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: African green monkey kidney epithelial BSC-1 cells (ATCC CCL-26) were maintained in Eagle’s minimal essential medium (EMEM; Lonza, Inc.) containing 5% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... mouse embryonic fibroblasts (MEFs) or African green monkey kidney fibroblasts (COS7) were cultured (37°C, 5% CO2) in DMEM (Lonza), supplemented with 10% fetal bovine serum (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... recombinant Cas9 nuclease (IDT) and the 4D-Nucleofector (Lonza) as previously described (Wagenblast et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... human recombinant insulin (20 μg/mL, Lonza, #BE03-033E20), human recombinant EGF (2 ng/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... The recombinant plasmid was transfected into HT29 cells using 4D-Nucleofecor (Lonza).
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bioRxiv - Biophysics 2020Quote: ... hydrocortisone and recombinant human epidermal growth factor (MEGM Bullet Kit CC-4136; Lonza). Cells were maintained in an incubator under standard conditions (36.5 °C ...
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bioRxiv - Bioengineering 2023Quote: The PyroGene Recombinant Factor C Endpoint Fluorescent Assay (Lonza, Walkersville, MD, cat # 50-658U) was performed as recommended ...
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bioRxiv - Immunology 2022Quote: ... Absence of endotoxins (< 1EU/mL) was confirmed using the PyroGene™ Recombinant Factor C Assay (Lonza) according to manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... RNPs containing recombinant CRISPR/Cas9 and sgRNA were transfected to human cell lines using a Nucleofector (Lonza). Stable cell lines were generated by selection of hygrogmycin resistance ...
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bioRxiv - Molecular Biology 2020Quote: ... Days 16-24 media contained recombinant Oncostatin M (20ng/ml, Thermo) in HCM media lacking EGF (Lonza).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 100 μg/ml streptomycin and 15 ng/ml recombinant human macrophage colony stimulating factor (Lonza, Melbourne, Australia) on 10 mm square dishes (Bio-strategy ...
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bioRxiv - Cell Biology 2023Quote: ... sgRNA and recombinant CAS9 were delivered as ribonucleoprotein (RNP) complexes using a 4D-Nucleofector X-Unit (Lonza). Briefly ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant plasmids were verified by Sanger sequencing and electroporated into E14 cells using the Nucleofector II platform (Lonza) (program A-013) ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μg ml−1 streptomycin and 15 ng ml−1 recombinant human macrophage colony stimulating factor (Lonza, Melbourne, Australia) on 10 mm square dishes (Bio-strategy ...
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bioRxiv - Bioengineering 2023Quote: ... gRNAs were complexed with recombinant Cas9 (IDT Cat#1081059) and introduced into cells by electroporation (4D-Nucleofector, Lonza Biosciences) according to manufacturer protocols ...
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bioRxiv - Genomics 2021Quote: ... 1.6×105 cells were suspended in P3 Primary Cell Solution with 50pmol Cas9 protein (St. Jude Protein Production Core) and 150pmol sgRNA (Synthego) with pMaxGFP plasmid at 200ng/ul (Lonza) in a total volume of 20ul with program DS130 ...
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bioRxiv - Genomics 2021Quote: ... and 50 pmole of spCas9 protein (St. Jude Protein Production Core) with pMaxGFP plasmid as a transfection control at 200ng/ul (Lonza) using the 4D-Nucleofector™ X-unit (Lonza ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were separated by SDS-PAGE using 14% Prosieve (Lonza) polyacrylamide gels and electroblotted to Immobilon-P membranes (Millipore) ...
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bioRxiv - Synthetic Biology 2023Quote: ... with Cas9 ribonucleic protein (RNP) complexes in SF buffer (Lonza), using the pulse codes CM-150 or CV-104 ...
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bioRxiv - Cancer Biology 2020Quote: ... consisting of 100 pmol of chemically modified sgRNA (Synthego) and 35 pmol of Cas9 protein (St. Jude Protein Production Core) via nucleofection (Lonza, 4D-Nucleofector™ X-unit) using solution P3 and program CA-137 in small cuvettes according to the manufacturers recommended protocol ...
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bioRxiv - Microbiology 2020Quote: ... the medium was removed and replaced by Ultradoma Protein-Free (LONZA). The cultured medium was harvested and replenished on the second ...
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bioRxiv - Genetics 2022Quote: ... membranes were stained with SYPRO-Ruby Protein Gel Stain (Lonza Rockland) to confirm equal loading ...
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bioRxiv - Immunology 2023Quote: The 3 kbp DNA plasmid encoding green fluorescent protein (GFP) (Lonza) was used to assess transfection efficiency ...
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bioRxiv - Cell Biology 2023Quote: ... S2Xpress cells were cultured in Insect-XPRESSTM protein-free medium (LONZA, Belgium) supplemented with 100 U/ml penicillin and 100 mg/ml streptomycin (GIBCO) ...
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bioRxiv - Immunology 2023Quote: ... Protein expression was induced with 5µM CdCl2 in Insect-XPRESS medium (Lonza) for 5-7 days and supernatants were concentrated and purified using strep-tacting affinity chromatography columns in an AKTA FPLC system ...
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bioRxiv - Immunology 2020Quote: ... both grown in Insect-XPRESS Protein-free Insect Cell Medium (Lonza, BE12-730Q) supplemented with L-glutamine (to 1%) ...
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bioRxiv - Microbiology 2020Quote: ... cultured in Insect-XPRESS Protein-free Insect Cell Medium supplemented with L-glutamine (Lonza). A Sf21 cell culture of ∼1.8 × 106 cells/ml was inoculated with the third passage stock of virus and incubated for three days at 28 °C ...
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bioRxiv - Immunology 2022Quote: ... the protein extraction was done with IP buffer (50 mM Hepes pH 7,5 (Lonza), 150 mM NaCl (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown in Insect-XPRESS™ Protein-free Insect Cell Medium (Lonza; 12-730Q) supplemented with antibiotic-antimycotic (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were maintained in Insect-XPRESS protein-free insect cell media with L-glutamine (Lonza) and infected at a density of 3-4 x 106 with high-infectivity baculovirus for protein expression.
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bioRxiv - Bioengineering 2022Quote: Human umbilical vein endothelial cells expressing green fluorescent protein (GFP-HUVECs) (C2519A, Lonza, Basel, Switzerland) were cultured in a supplemented (EGM-2 bullet kit ...
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bioRxiv - Bioengineering 2024Quote: Human umbilical vein endothelial cells expressing green fluorescent protein (GFP-HUVECs) (C2519A, Lonza, Basel, Switzerland) were cultured in a supplemented (EGM-2 bullet kit ...
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bioRxiv - Immunology 2019Quote: ... the ribonucleoprotein (RNP) complex was first formed by incubating 10μg of TrueCut Cas9 Protein V2 (Lonza) with 5μg of sgRNA for 10-30min at room temperature ...
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bioRxiv - Plant Biology 2020Quote: Ubiquitinated GST-SYP61 proteins were separated by SDS-PAGE and stained with SYPRO Ruby (Lonza, Switzerland) as described in the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies were validated with Western blot analysis using protein from Neuronal Human Astrocytes (Lonza, CC-2565) as previously reported in Knight and Serrano (2017a).
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bioRxiv - Cell Biology 2023Quote: ... were cultured at 27°C in Insect-XPRESS protein-free insect medium with L-Glutamine (Lonza) in shaking suspension ...
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bioRxiv - Bioengineering 2023Quote: ... 2×109 Freestyle CHO-S cells were resuspended in 500ml ProCHO 4 Protein-free Medium (Lonza, Cat#BEBP12-029 ...
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bioRxiv - Immunology 2023Quote: ... a ribonucleoprotein (RNP) complex was first formed by incubating 5µg of TrueCut Cas9 Protein V2 (Lonza) with 10µg of sgRNA for 10 min at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... the total protein was size-separated on an 8% polyacrylamide gel and stained with SYPRO Ruby (Lonza). When the contents of the protein preparation were analyzed by western blotting ...
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bioRxiv - Cell Biology 2023Quote: ... Sf21 cells were grown as a monolayer culture in Insect-XPRESS Protein-free Insect Cell Medium (Lonza) in standard T175 culture flasks at 27 °C ...
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bioRxiv - Biophysics 2019Quote: ... 1 L of Sf9 insect cells in Insect-XPRESS Protein-free Insect Cell Medium with L-glutamine (Lonza) in a 3 L flask was infected with 5 ml of the harvested P2 (second passage ...
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bioRxiv - Immunology 2022Quote: ... and protein expression was carried out in suspension Trichoplusiani ni (High Five) cells grown in InsectXpress media (Lonza).
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bioRxiv - Cancer Biology 2020Quote: CRISPR/Cas9 genome editing was performed by ribonucleotide-protein (RNP) nucleofection using the 4D-Nucleofector™ System (Lonza), following the electroporation conditions already optimized in the laboratory ...
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bioRxiv - Immunology 2022Quote: ... used for baculovirus preparation were maintained in Insect-XPRESSTM Protein-free Insect Cell Medium with L-Glutamine (Lonza) at 28°C under agitation ...
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bioRxiv - Cell Biology 2023Quote: ... with a blue fluorescent protein (BFP)-tag were cultured in smooth muscle cell growth medium-2 (SMGM2) (Lonza). Human umbilical cord vein endothelial cells (HUVEC ...