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Citations for Lonza :
1 - 50 of 1888 citations for Rat Small Nuclear Ribonucleoprotein U5 Subunit 200 SNRNP200 ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Ribonucleoprotein (RNP) complexes were delivered by Human T Cell NucleofectorTM Kit (Lonza, Basel, Switzerland). Both TCR chains were targeted by two crRNAs ...
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bioRxiv - Microbiology 2021Quote: ... were gel purified and the linear fragment flanked by the small subunit rRNA (SSU) homology regions was transfected into log-phase promastigotes using an AMAXA Nucleofector 4D (Lonza). Parasites (8 × 106 cells ...
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bioRxiv - Neuroscience 2024Quote: ... ReN cells VM were electroporated with ribonucleoprotein complex using the nucleofector P3 kit (Lonza, V4XP-3032). Single cell colonies were generated by limited dilution in 96-well plates ...
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bioRxiv - Cell Biology 2024Quote: ... the Ribonucleoprotein (RNP) complexes (9:1 sgRNA to Cas9 ratio) were assembled in Nucleofector Solution plus Supplement (Lonza Amaxa HMEC Nucleofector Kit) to a total volume of 100µL ...
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bioRxiv - Immunology 2023Quote: ... a ribonucleoprotein (RNP) complex was first formed by incubating 5µg of TrueCut Cas9 Protein V2 (Lonza) with 10µg of sgRNA for 10 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... sgRNA and recombinant CAS9 were delivered as ribonucleoprotein (RNP) complexes using a 4D-Nucleofector X-Unit (Lonza). Briefly ...
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bioRxiv - Genomics 2021Quote: ... Primary small airway cultures were maintained in Small Airway Growth Media (Lonza, Basel, Switzerland) supplemented with 10ng/mL keratinocyte growth factor (KGF) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.5 uL of 2ug/uL ABE8e-NRCH ribonucleoprotein editor,83 1uL of base editor primer (50uM stock) and 3.6uL of Nucleofector supplement (Lonza). The ABE8e-NRCH base editor was a kind gift from Dr ...
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bioRxiv - Bioengineering 2024Quote: Ribonucleoprotein (RNP) complexes were delivered into HEK293T cells via a 4D-Nucleofector® X Unit (Lonza, Cat# AAF-1003X) in a strip format using SF Cell Line 4D-Nucleofector™ X Kit S (Lonza ...
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bioRxiv - Cell Biology 2022Quote: ... Large and small airway epithelial cultures were maintained in Small Airway Growth Media (CC-4124, Lonza) supplemented with 5% FBS and 10 ng/ml KGF (keratinocyte growth factor) ...
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bioRxiv - Microbiology 2020Quote: ... SAEC (Small Airway Epithelial cells, Lonza) were grown in Small Airway Epithelial Cell Growth Basal Medium (SABM ...
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bioRxiv - Immunology 2021Quote: ... Small airway epithelial cells (SAECs; Lonza) were cultured and maintained according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... was combined with 15 pmol total synthetic sgRNA (5 pmol each sgRNA) (Synthego) to form ribonucleoproteins (RNPs) in 20uL total volume with SE Buffer (Lonza). The RNP assembly reaction was mixed by pipetting up and down and incubated at room temperature for 10 minutes.
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bioRxiv - Neuroscience 2022Quote: ... of CLN3 iPSCs was electroporated with Cas9-sgRNA ribonucleoprotein (IDT) and 966 bp donor plasmid using Amaxa 4D Nucleofector (Lonza) with program CB 150 ...
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bioRxiv - Biochemistry 2022Quote: ... 2×105 143B or HEK293T cells were resuspended in the resulting solution containing ribonucleoprotein complexes (RNPs) and electroporated using a 4D-Nucleofector (Amaxa, Lonza) programs FP-133 (143B ...
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bioRxiv - Immunology 2022Quote: ... IFNAR KO A549 cells were generated by CRISPR-Cas9 ribonucleoprotein (RNP) complex (IDT) transfection using the Nucleofector system (Lonza Bioscience). Specifically ...
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bioRxiv - Biochemistry 2022Quote: ... and 18-36 μl ribonucleoprotein complexes were added along with 4 μM Alt-R Cas9 electroporation enhancer (IDT) to a nucleofection chamber (Lonza). Cells were electroporated using the nucleofector program EN-138 and gently resuspended in DMEM ...
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bioRxiv - Immunology 2022Quote: ... T cells were then electroporated with ribonucleoprotein (RNP) complexes of sgRNA and SpCas9 in 100 μL cuvettes using pulse code EH111 of the 4D nucleofector (Lonza). RNP complexes were generated by incubating 5 ug of chemically modified sgRNA with 10 ug HiFi SpCas9 for 15 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: Tumour cells were genetically edited by electroporating the Cas9/crRNA/tracrRNA or Cas9/sgRNA ribonucleoprotein (RNP) complexes into cells using the 4D-nucleofector system (Lonza). To prepare the guide RNA complex ...
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bioRxiv - Immunology 2022Quote: ... 1×106 cells were electroporated (pulse code EH100) with Cas9 ribonucleoprotein and DNA templates in 20 ul Nucleofector Solution P3 (Lonza) with a 4D Nucleofector X unit (Lonza) ...
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bioRxiv - Biochemistry 2023Quote: ... 200,000 H1299 cells were resuspended in the resulting solution containing ribonucleoprotein complexes (RNPs) and electroporated using a 4D-Nucleofector (Amaxa, Lonza). Nucleofected cells were then expanded and single-cell cloned by limiting dilution by plating 0.5 cells/well in a 96 well plate ...
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bioRxiv - Genetics 2023Quote: ... the assembled ribonucleoprotein complexes were added to non-attached primary hepatocytes in suspension at 1x106 cells / mL in HCM media (Lonza). Cells were incubated with rocking at 37°C for 2 hours before transplantation ...
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bioRxiv - Immunology 2023Quote: ... of target genes in primary human T cells were generated by Cas9 ribonucleoprotein (RNP) electroporation using a 4D Nucleofector (Lonza) and P3 primary cell nucleofection reagents (Lonza ...
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bioRxiv - Cell Biology 2023Quote: Pooled knockout cell lines were generated by electroporating Cas9-guide RNA ribonucleoprotein complexes into reporter K562 cells using an Amaxa 4D Nucleofector X Unit (Lonza) and the SF Cell Line 4D-Nucleofector X Kit S (Lonza) ...
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bioRxiv - Cancer Biology 2024Quote: ... were annealed to Cas9 protein (IDT) and the ribonucleoprotein complex was transfected into SKOV-3 cells using a 4D-Nucleofector (Lonza). Bulk transfected cells were checked by flow cytometry for successful knock-down ...
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bioRxiv - Cell Biology 2024Quote: ... Cell clumps were electroporated using Mouse/Rat Hepatocyte NucleofectorTM Kit (Lonza) and Amaxa Nucleofector® 1 device in the presence or absence of flagellin (1μg ...
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bioRxiv - Systems Biology 2024Quote: ... MT2 and MDA-MB-231 cells with HLA-A,B,C CRISPR-Cas9 knockout were generated through electroporation of Cas9-sgRNA ribonucleoprotein (RNP) complex using 4D-Nucleofector (Lonza Bioscience). Three sgRNAs targeting HLA gene (HLA sgRNA#1 ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... MDA-MB-231 and PC3 cells with FBXO22 CRISPR-Cas9 knockout were generated through electroporation of Cas9-sgRNA ribonucleoprotein (RNP) complex using 4D-Nucleofector (Lonza Bioscience). Two sgRNAs targeting FBXO22 gene (FBXO22 sgRNA#1 ...
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bioRxiv - Cancer Biology 2024Quote: ... The media is comprised of small airway basal media (SABM) with selected components from SAGM bullet kit (Lonza) including Insulin ...
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bioRxiv - Microbiology 2024Quote: ... adult jejunum (J2) and adult small intestine (Lonza), were maintained at 37°C with 5% CO2 in extracellular matrix (Matrigel ...
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bioRxiv - Cell Biology 2021Quote: Exocyst subunits and PIP5K1C were expressed in SF9 cells cultured in Insect-EXPRESS (Lonza) by baculovirus mediated expression ...
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bioRxiv - Neuroscience 2022Quote: ... Nucleofected striatal neurons were transfected using manufacturer’s instructions (Rat Neuron Nucleofector Kit, Lonza) for rat hippocampal neurons before plating ...
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bioRxiv - Developmental Biology 2024Quote: ... The cell suspension was transferred into the electroporation cuvette (Lonza rat nucleofector kit) while avoiding bubbles ...
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bioRxiv - Bioengineering 2021Quote: ... Small Airway Epithelial Cell Growth Basal Media (SABM, Lonza) was mixed with Insulin/Transferrin ...
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bioRxiv - Cell Biology 2020Quote: ... Human small airway epithelial cells (SAECs, Lonza, CC-2547) were cultured in PneumaCult-Ex Plus medium (Stemcell Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... Fluorescent nuclear counterstaining was performed using DAPI (Lonza, cat#: PA3013).
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bioRxiv - Cell Biology 2020Quote: ... Modified MTEC culture media is comprised of small airway basal media (SABM) with selected components from SAGM bullet kit (Lonza) including Insulin ...
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bioRxiv - Neuroscience 2022Quote: ... cell pellet was resuspended in 100 ml Nucleofector™ (Rat Neuron Nucleofector kit, Lonza) transfection reagent and electroporated with 2 μg of cDNA mix according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2024Quote: Small Airway Epithelial Cells were purchased from Lonza (CC-2547). To expand ...
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bioRxiv - Cell Biology 2024Quote: ... and 50% Small Airway Epithelial Cell Growth Media (SAGM; Lonza) and plated in a 24-well Falcon Cell Culture Insert (Falcon) ...
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bioRxiv - Developmental Biology 2022Quote: ... SABM Small Airway Epithelial Cell Growth Basal Medium (Lonza, CC-3119) was combined with the following additives ...
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bioRxiv - Bioengineering 2023Quote: ... Primary small airway epithelial cells were purchased from Lonza (CC-2547) and expanded in Lonza’s small airway growth medium with BulletKit supplements as instructed (CC-3118) ...
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bioRxiv - Cell Biology 2023Quote: ... cultured in small airway basal medium (SABM, cat. no.CC-3118, Lonza) with BPE low protein ...
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bioRxiv - Cell Biology 2024Quote: Rat primary hepatocytes (Lonza, #RWCP01) were thawed and plated in a 24-well plate at a density of 0.16 million cells per well in Hepatocyte Plating Medium (Lonza ...
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bioRxiv - Molecular Biology 2020Quote: Primary human small airway epithelial cells were obtained from Lonza (Walkersville, MD) and PromoCell (Heidelberg ...
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bioRxiv - Microbiology 2020Quote: ... re-suspended in complete small airway epithelial cell growth media (SAGM; Lonza), 100 U/mL penicillin-streptomycin (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... Stable pools of cells were maintained in small airway growth medium (Lonza).
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bioRxiv - Microbiology 2020Quote: ... were grown in Small Airway Epithelial Cell Growth Basal Medium (SABM, Lonza) and Small Airway Epithelial Cell Growth Medium SingleQuots Supplements and Growth Factors (Lonza).
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bioRxiv - Systems Biology 2023Quote: ... and grown in SAGM small airway growth medium (Lonza, Walkersville, MD, USA). All cells were incubated at 37 °C ...
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bioRxiv - Genetics 2023Quote: ... approximately 75,000 U2OS cells were nucleofected into small cuvettes (V4SP-3096, Lonza) with 20µl of P3 Primary Cell line solution (V4SP-3096 ...