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Citations for Lonza :
201 - 250 of 3898 citations for Rat Carboxypeptidase A3 Mast Cell CPA3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... cells were resuspended in 20 µL of P3 Primary Cell Nucleofector solution (Lonza #V4SP-3096) and mixed gently with 4 µL of NTC complexes ...
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bioRxiv - Cell Biology 2023Quote: ... Electroporation mix was prepared resuspending RPE1 cells in P3 Primary Cell Full Electroporation Buffer (Lonza), and adding previously prepared RNPs ...
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bioRxiv - Microbiology 2023Quote: ... HeLa cells (human epithelial cell line) were cultured in DMEM - Dulbecco’s Modified Eagle Medium (Lonza) supplemented with 10% FBS (Gibco ...
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bioRxiv - Genetics 2023Quote: ... Cells were electroporated using the SF Cell Line 4D-Nucleofector X Kit (V4XC-2012; Lonza) with 8 μg of each guide plasmid and the K562 cell line recommended protocol (FF-120) ...
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bioRxiv - Bioengineering 2023Quote: ... the MC3T3-E1 osteoblast cell line (ATCC) and human umbilical vein endothelial cells (HUVECs, Lonza). MLO-Y4 cells were maintained in α-MEM supplemented with 5% fetal bovine serum (FBS) ...
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bioRxiv - Bioengineering 2023Quote: ... Human umbilical vein endothelial cells (HUVECs) were expanded in Endothelial Cell Growth Medium-2 (Lonza), harvested for use between passage P4- P6 ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid transfection in U2OS cells was done using Amaxa Cell Line Nucleofector Kit V (Lonza) according to manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... and primary T cells were electroporated using the P3 Primary Cell 4-D Kit (Lonza). For Cas9 and sgRNA delivery ...
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bioRxiv - Microbiology 2023Quote: RAW264.7 cells (murine macrophage cell line) were cultured in DMEM - Dulbecco’s Modified Eagle Medium (Lonza) supplemented with 10% FBS (Gibco ...
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bioRxiv - Genomics 2023Quote: ... The cells were washed once with PBS and resuspended in P3 Primary Cell solution (Lonza). RNP complexes were formed by mixing Cas9 protein (IDT ...
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bioRxiv - Developmental Biology 2024Quote: FSPS13b hiPS cells were nucleofected using the P3 Primary Cell NucleofectorTM Solution (Lonza #V4XP-3024) with pulse code CA137 ...
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bioRxiv - Microbiology 2020Quote: ... major promastigotes expressing Cas9 and T7 RNA polymerase proteins [30] were co-transfected with donor DNA and sgRNA templates using V-033 program of Amaxa Nucleofector System (Lonza). Transfected parasites were selected with 50 μg/ml neomycin and cloned in semi-solid culture medium ...
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bioRxiv - Bioengineering 2021Quote: Primary human umbilical vein endothelial cells (HUVECs) and red fluorescent protein-expressing HUVECs (RFP-HUVECs) were maintained in EMG-2 medium (complete EGM-2 BulletKitTM, Lonza). We obtained human umbilical cords from Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine ...
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bioRxiv - Cell Biology 2020Quote: ... The purity of CyaA batches is higher than 90% as judged by SDS PAGE analysis and contained less than 1 EU of LPS/μg of protein as determined by a standard LAL assay (Lonza). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... Western blots were conducted by running equivalent amounts of protein on 4-20% Tris-Glycine SDS/polyacrylamide gradient gels (Lonza) after reduction with 2-mercaptoethanol and heating to 95°C for 5m ...
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bioRxiv - Immunology 2021Quote: ... for RNP formation 180 pmol crRNA-tracrRNA duplex was mixed with 60 pmol Cas9 protein in Nucleofection Buffer P3 (Lonza) and incubated for at least 10 min at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... This cocktail was provided to the cells together with a ribonucleoprotein complex consisting of crRNA, tracrRNA and Cas9 protein (IDT, refer to “Oligonucleotides” for crRNA sequence) via nucleofection using a 4D nucleofector (Lonza). Next ...
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bioRxiv - Physiology 2023Quote: ... 2 μl of KCNQ2/3 cDNA plasmid (0.9 g/l) and 1 μl of pmax green fluorescent protein vector (0.5 g/l) (Lonza, Cologne, Germany) were diluted in 125 μl of Opti-MEM medium (Life Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... and 2000 p mol of a degron-tagged control protein (mTagBFP2-RxxG) in a 100 µl nucleofection reaction (4D nucleofector kit SE plus supplement SF1, Lonza) 14 hours following nucleofection ...
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bioRxiv - Microbiology 2021Quote: ... and 10^6 cells were transfected into cells using Amaxa 96-well Nucleofector (Lonza, Basel, Switzerland) SF kit on program DS-138 ...
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bioRxiv - Bioengineering 2020Quote: ... cells were cultured in human T cell media comprised of X-VIVO 10 (Lonza #04-380Q), 5% human AB serum (Valley Biomedical #HP1022) ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured in human T cell medium (hTcm) consisting of X-VIVO 15 (Lonza) and 5% Human AB serum (Gemini ...
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bioRxiv - Cancer Biology 2020Quote: All plasmids were transfected into 106 cells using Cell Line Nucleofector Kit V (Lonza, Basel, Switzerland) and the T-013 program of a Nucleofector 2b Device (Lonza ...
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bioRxiv - Cell Biology 2020Quote: ... Jurkat T cells were transfected using the Amaxa Cell Line Nucleoefector Kit V (Lonza, VCA-1003). Transient transfections were performed as follows ...
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bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMCs) and peripheral blood CD4-positive cells were purchased from Lonza (product numbers CC-2702 and 2W-200 ...
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bioRxiv - Immunology 2022Quote: NK cells were centrifuged and resuspended in P3 Primary Cell Nucleofector© Solution (Lonza, V4XP-3032) at 3.33×108 cells/mL ...
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bioRxiv - Biochemistry 2022Quote: ... Exponentially growing Spodoptera frugiperda 9 cells (2E06 cells/mL in Lonza Insect-XPRESS medium #BELN12-730Q) were infected with high-titer Baculovirus suspension ...
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bioRxiv - Synthetic Biology 2022Quote: ... ∼1.0×106 cells were transfected by Amaxa Cell Line Nucleofector Kit V (VCA-1003, Lonza, Switzerland) following the manufacturer’s instructions (2D ...
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bioRxiv - Cancer Biology 2020Quote: Single cells were suspended in X-VIVO 10 Serum-free Hematopoietic Cell Medium (#04-380Q, Lonza) in six-well Ultra-Low Attachment Plates (Corning) ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were electroporated in cuvettes (electroporation volume of 100 μL) using Human CD34+ Cells kit (Lonza) and U08 electroporation program ...
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bioRxiv - Neuroscience 2021Quote: ... Pelleted cells were resuspended in 100 μL of complete P3 Primary Cell Solution (Lonza #V4XP-3024), then mixed with the RNP/ssODN by trituration ...
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bioRxiv - Immunology 2020Quote: ... Cells were filter through a 100 μm nylon cell strainer (Falcon) and cultured in RPMI (Lonza) supplemented with 20% L929-cell conditioned medium ...
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bioRxiv - Genetics 2021Quote: ... Cells were transfected using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza, V4XP-3024) with pulse code DS138 ...
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bioRxiv - Cancer Biology 2020Quote: ... Endothelial cells (104 cells/well) were treated with compounds in fully supplemented EGM-2 medium (LONZA) for 48 h and then analyzed as above.
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bioRxiv - Bioengineering 2022Quote: ... 20 μL of T cells were resuspended in P3 buffer at 5 × 107 cells/mL (Lonza) and added to the electroporation mixture ...
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bioRxiv - Microbiology 2022Quote: ... Pelleted cells were resuspended in SF Cell Line Nucleofector Solution with added supplement (Lonza catalog # V4XC2024). 25 µg of the plasmid to be transfected (Aldevron ...
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bioRxiv - Microbiology 2022Quote: ... BEAS-2B cells were cultured in Bronchial Epithelial Cell Growth Medium (BEGM, Lonza, Kingston, ON, Canada) on plates prepared with coating media (0.01 mg/ml fibronectin ...
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bioRxiv - Microbiology 2023Quote: ... for 20h and fibroblasts cells were nucleofected using the SE Cell Line AD-Nucleofector Kit (Lonza). Knock-down procedures were performed with siRNA purchased from Riboxx (non-targeting siRNA ...
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bioRxiv - Immunology 2023Quote: Human Umbilical Vein Cells (HUVECs) were cultured in EBM Endothelial Cell Growth Basal Medium (Lonza, Swiss) supplemented with EGMTM Endothelial Cell Growth Medium SingleQuots (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids and siRNAs were introduced into chromaffin cells (5 × 106 cells) by Amaxa Nucleofactor systems (Lonza) according to manufacturer’s instructions and as described previously [46] ...
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bioRxiv - Cancer Biology 2023Quote: Human peripheral blood mononuclear cells (PBMCs) and peripheral blood CD4-positive cells were purchased from Lonza (product numbers CC-2702 and 2W-200 ...
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bioRxiv - Bioengineering 2023Quote: ... This mixture was electroporated into cells using the P3 Primary Cell 4D-Nucleofector X kit (Lonza) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells (200,000 cells per each experimental group) were nucleofected with 4D-Nucleofector (Amaxa-Lonza, Basel, Switzerland) in 20 µl of SG solution ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were trypsinized and resuspended in endothelial cell growth basal medium-2 (EBM-2, Lonza, Switzerland). Cell seeding density into wells was 0.6 × 105 cells in 300μl medium ...
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bioRxiv - Bioengineering 2024Quote: ... Human microvascular endothelial cells (hMVEC) and human umbilical vein endothelial cells (hUVEC) were obtained from Lonza (hMVEC CC-2543 ...
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bioRxiv - Bioengineering 2024Quote: ... 5 million cells were suspended in 74 μl of P4 Primary Cell solution (Lonza, VSOP-4096). In parallel ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were transfected using Nucleofection (Lonza) as recommended by the manufacturer (Amaxa) ...
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bioRxiv - Biochemistry 2020Quote: ... cell culture reagents were from Lonza and Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: Human mesenchymal stromal cells (hMSCs, Lonza) were used at passage 7 for all experiments ...
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bioRxiv - Bioengineering 2020Quote: Bone marrow stromal cells (BMSC, Lonza) were cultured in Myelocult 5100 (StemCell Technologies ...