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Citations for Lonza :
301 - 350 of 2536 citations for Protein Kinase A PKA Activity Assay Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: hMSC were plated at 15,000 cells/well in 24-well plates and grown overnight in a growth medium containing 10% FBS (Lonza). When cells reach 80% confluency ...
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bioRxiv - Immunology 2020Quote: ... The MycoAlert Mycoplasma Detection Kit (Lonza) was used to monitor mycoplasma contamination on a regular basis.
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bioRxiv - Bioengineering 2019Quote: ... and EGM-2 bullet kit (Lonza)) and maintained at ALI for three weeks.
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bioRxiv - Developmental Biology 2019Quote: ... using a HUVEC Nucleofector kit (Lonza) according to manufacturer’s instructions and the cells were further cultured for 72 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... using the MycoAlert Kit (Lonza, Switzerland). NIH3T3 mouse embryonic fibroblasts were stably transfected with pCMV_HER4 that encodes the JMa/CYT1 isoform ...
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bioRxiv - Immunology 2021Quote: ... and Nucleofector kits (Lonza, VPB-1002) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... MycoAlert Mycoplasma Detection Kit (Lonza, Bioscience). Each cell lines were used within passage 4/5 since their thawing from originally frozen vials.
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bioRxiv - Cancer Biology 2022Quote: ... Mycoplasma testing (MycoAlert Detection Kit, Lonza) was performed monthly.
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bioRxiv - Cell Biology 2022Quote: ... using MycoAlert detection kit (Lonza, LT07). Additionally ...
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bioRxiv - Cancer Biology 2022Quote: ... Regular testing with MycoAlert kit (Lonza) confirmed the absence of mycoplasma contamination ...
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bioRxiv - Cancer Biology 2020Quote: ... using MycoAlert™ kit (Lonza, Germany) and genetically authenticated using a STR-PCR fragments kit (Agilent Technologies ...
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bioRxiv - Immunology 2019Quote: ... Kit T solution (Lonza, Basel, Switzerland), and programs G-016 ...
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bioRxiv - Immunology 2021Quote: ... Nucleofector Kit V was from Lonza. The original vector pCasper-GR (#FP971 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with the SingleQuots kit (Lonza), 5 μg/ml transferrin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Nucleofector Kit V (Lonza, VCA-1003); paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2023Quote: ... with the full growth kit (Lonza) and passaged using TrypLE Express.
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bioRxiv - Molecular Biology 2023Quote: ... Mycoplasma detection kit (Lonza #LT07-318) was used as per the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: Cell□line□specific Nucleofector Kits (Lonza) were used for transfections which were performed according to manufacturer’s protocol on a Nucleofector® 2b Device (Lonza) ...
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bioRxiv - Cell Biology 2023Quote: ... using MycoAlert detection kit (Lonza, LT07). For co-immunoprecipitation experiments ...
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bioRxiv - Cell Biology 2023Quote: ... and verified mycoplasma free by Lonza MycoAlert mycoplasma detection kit (LT07, Lonza). Fibroblasts were synchronized by temperature cycles of 12 h 32°C followed by 12 h 37°C for 5 days ...
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bioRxiv - Microbiology 2024Quote: Toxilight Bioassay Kit (Lonza, LT17-127) or LDH-Glo Cytotoxicity Assay (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lines were routinely validated by functional assay and western blot as appropriate and tested monthly for mycoplasma contamination by MycoAlert (Lonza). Transfection of plasmid DNA was performed with Lipofectamine 3000 and P3000 reagent (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... and human ACTB (assay control, primer composition not disclosed by manufacturer) were loaded on a 2.5% agarose gel (Lonza, 50010) and stained with GelRed® (Merck ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines used in this study were authenticated using short tandem repeat genotyping (Labcorp) and confirmed to be negative for mycoplasma contamination using MycoAlertTM PLUS Assay (Lonza).
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bioRxiv - Immunology 2020Quote: ... Endotoxin concentrations ranged from 150-175 EU/mL as determined using the limulus amebocyte lysate assay (Lonza, Walkersville, MD, USA). This concentration of ODE has been previously shown to produce optimal experimental effects and is well-tolerated in mice (11 ...
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bioRxiv - Cell Biology 2023Quote: ... All sorted and parental cell lines were confirmed to be mycoplasma-negative in a MycoAlert PLUS assay (Lonza, LT07-710) performed by the Koch Institute High-Throughput Sciences Facility (Cambridge ...
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bioRxiv - Cell Biology 2023Quote: ... BM CD34+ cells from young healthy donors for functional assays were collected at San Raffaele Hospital or purchased from Lonza. For the phenotypic analyses the sample size was determined by the number of individuals for whom excess material was available ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were confirmed to be mycoplasma-free based on a luminescence-based enzymatic assay (MycoAlert®, Lonza, Cat# LT07-118) conducted within 1 week of the commencement and completion of the experiments ...
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bioRxiv - Developmental Biology 2020Quote: ... a 1:1 mix containing EGM2-Incomplete (Lonza) and macrophage media (v/v ...
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bioRxiv - Immunology 2019Quote: ... the ribonucleoprotein (RNP) complex was first formed by incubating 10μg of TrueCut Cas9 Protein V2 (Lonza) with 5μg of sgRNA for 10-30min at room temperature ...
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bioRxiv - Plant Biology 2020Quote: Ubiquitinated GST-SYP61 proteins were separated by SDS-PAGE and stained with SYPRO Ruby (Lonza, Switzerland) as described in the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies were validated with Western blot analysis using protein from Neuronal Human Astrocytes (Lonza, CC-2565) as previously reported in Knight and Serrano (2017a).
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bioRxiv - Bioengineering 2023Quote: ... 2×109 Freestyle CHO-S cells were resuspended in 500ml ProCHO 4 Protein-free Medium (Lonza, Cat#BEBP12-029 ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured at 27°C in Insect-XPRESS protein-free insect medium with L-Glutamine (Lonza) in shaking suspension ...
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bioRxiv - Immunology 2023Quote: ... a ribonucleoprotein (RNP) complex was first formed by incubating 5µg of TrueCut Cas9 Protein V2 (Lonza) with 10µg of sgRNA for 10 min at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were mixed at designated ratios in a 96-well transparent bottom plate in X-Vivo 15 without phenol red (Lonza) supplemented with FBS (Gibco ...
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bioRxiv - Biochemistry 2019Quote: ... at passages 4-7 were grown to confluence on gelatin-coated plates and maintained using complete EGM-2 media (Lonza). HKi002 was maintained at room temperature for 30 min rotating before use ...
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bioRxiv - Biochemistry 2019Quote: hCMEC/D3 cells at passages 30–37 were grown to confluence on collagen-coated plates and cultured using in EGM-2 MV medium and supplemented with complements obtained from the manufacturer (Lonza) as previously reported (37) ...
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bioRxiv - Cell Biology 2020Quote: ... The cell / Amaxa solution mixture was added to the RNP mix and then pipetted into the bottom of a 96-well nucleofection plate (Lonza). This sample was then nucleofected using the 4D-Nucleofector Core unit and 96-well shuttle device (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... fumigatus collected from three 10-cm plates grown for 7 days at 37°C were germinated with shaking in RPMI 1640 (Lonza) liquid media supplemented with glucose to a final concentration of 2% (w/v ...
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bioRxiv - Developmental Biology 2022Quote: Primary HDLECs were isolated from neonatal human foreskins as described previously (74) and cultured from passages 5 – 7 on fibronectin-coated plates with EGM-2 MV growth media (Lonza) supplemented with human VEGF-C (R&D) ...
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bioRxiv - Genomics 2022Quote: ... hiPSC-derived epicardial cells were dissociated with Accutase and plated at a density of 104 cells/cm2 in 6-well plates and cultured in fibroblast growth medium (Lonza) supplemented with 20 ng/ml FGF2 and 10 μM SB431542 ...
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bioRxiv - Immunology 2019Quote: ... 750,000 cells per well were mixed with 6 μL of RNP per well and electroporated in a 96-well electroporation plate (Lonza) using electroporation code EH115 on a 4D Nucleofector system with 96-well shuttle (Lonza) ...
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bioRxiv - Bioengineering 2021Quote: ... SK-N-BE(2) or H460 cells were seeded at densities mentioned above in ultra-low adherent round-bottom 96-well plates (Lonza) for 72 hours ...
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bioRxiv - Neuroscience 2020Quote: ... Effects of PI3K inhibition on Syk phosphorylation in mouse WT primary microglia was studied by plating 100 000 cells in 96-well plate in Dulbecco’s modified Eagle’s medium (DMEM, Lonza, Verviers, Belgium) supplemented with 2mM L-glutamine (Lonza ...
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bioRxiv - Cell Biology 2021Quote: ... 5,000 cells were plated in low-attachment 96-well plates containing 100 µL MEGM media (without BPE) (Lonza, CC-3150), 20 ng/mL FGF (Sigma Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... or media were cultured in 96 well flat bottom plates overnight then PBMC (2×105) from healthy donors were cultured at 37 °C in X-VIVO 15 media (Lonza) with 50-200 U/mL IL-2 (Peprotech) ...
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bioRxiv - Cell Biology 2022Quote: ... Three slices were placed in one well of a 12-well plate and incubated with 2 mL DMEM (Lonza, #42430) with 0.6% amphotericin B ...
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bioRxiv - Microbiology 2022Quote: ... Nuli-1 cells were cultured on Collagen IV coated plates as previously described in Bronchial Epithelial Cell Growth Media (BEGM) with supplements (Lonza) (Zabner et al. ...
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bioRxiv - Physiology 2022Quote: HUVEC were grown at 80% confluence (5×103 cells per well) on glass coverslips in a 24-well plate and starved overnight in serum-free medium (EBM-2, Lonza). They were then incubated for 24 hr in EGM2 containing 10μM BrdU ...