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Citations for Lonza :
151 - 200 of 1661 citations for Protein Dialysis kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... using the Amaxa Human T Cell Nucleofector Kit (Lonza) and the Nucleofector 2b Device (Lonza ...
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bioRxiv - Cell Biology 2021Quote: ... Renal Cell Growth Medium (REGM) SingleQuot kit supplements (Lonza), 2.5μg/ml amphotericin B ...
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bioRxiv - Cancer Biology 2022Quote: ... SF Cell Line 4D-Nucleofector™ x Kit (Lonza) were used ...
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bioRxiv - Cancer Biology 2022Quote: ... All cells were routinely tested (MycoAlert Detection Kit, Lonza) and confirmed to be free of mycoplasma contamination.
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bioRxiv - Molecular Biology 2022Quote: ... and P3 primary kit (Lonza, catalog no. V4XP-3024) as per manufacturer’s instructions and plated out on matrigel coated 6-well plate using mTeSR with CloneR supplement ...
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bioRxiv - Microbiology 2022Quote: ... and the P3 primary cells kit (Lonza; V4XP-3024) as previously described (81 ...
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bioRxiv - Developmental Biology 2023Quote: ... using P3 Primary Cell 4D-Nucleofector X kit (Lonza). Transfected cells were plated onto irradiated DR4 MEFs (the Cell Bank of the Chinese Academy of Sciences ...
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bioRxiv - Developmental Biology 2023Quote: ... using P3 Primary Cell 4D-Nucleofector X kit (Lonza). Transfected cells were plated onto irradiated DR4 MEFs (the Cell Bank of the Chinese Academy of Sciences ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... using RBL-specific Amaxa Nucleofector transfection kit T (Lonza). Some samples ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... using RBL-specific Amaxa Nucleofector transfection kit T (Lonza). In this experiment ...
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bioRxiv - Cancer Biology 2023Quote: ... using the MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza). MDA-MB-231 cells were maintained in RPMI 1640 media supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... supplemented with the EGM-2 MV SingleQuots kit (Lonza). For culturing of primary lymphocytes ...
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bioRxiv - Biochemistry 2023Quote: ... MCF-10A [MEBM supplemented with Kit CC-3150 (Lonza) and 100 ng/mL cholera toxin (Sigma)] ...
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bioRxiv - Cell Biology 2023Quote: — MycoAlert Mycoplasma Detection Kit (Lonza cat. no. LT07-118)
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bioRxiv - Cancer Biology 2023Quote: ... with an SF kit (Catalog: V4CX-2032, Lonza Biosciences) as per manufacturer instructions using pulse code CM-120 ...
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bioRxiv - Cell Biology 2023Quote: ... using the MycoAlert™ Detection Kit (Lonza, #LT07-418) and MycoAlert™ Control Set (Lonza ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were mycoplasma negative (MycoAlert kit, Lonza). Experiments were performed using charcoal-stripped serum (CTS) ...
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bioRxiv - Cell Biology 2023Quote: ... in combination with Nucleofector Kit V (Lonza, VCA-1003) according to the manufacturer’s recommended protocol.
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bioRxiv - Cancer Biology 2023Quote: ... and supplemented with Fibroblast Growth Kit Serum-Free (Lonza) to improve growth and viability in dialyzed FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... checked monthly using the MycoAlert Mycoplasma Detection Kit (Lonza). Tumour-derived cell lines were confirmed to match original samples by STR fingerprinting ...
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bioRxiv - Immunology 2024Quote: ... with an Amaxa Mouse Dendritic Cell Nucleofection Kit (Lonza). The abovementioned pIRES2-AcGFP1-series plasmids were used for transient expression ...
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bioRxiv - Cell Biology 2024Quote: ... with the P3 Nucleofector kit (Lonza, Cat. # V4SP-3096). Naïve murine CD8+ T cells (2 x 106 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell Line NucleofectorTM Kit V (Catalog #: VCA-1003) (Lonza) was used for transfection procedure according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... using the MycoAlert Mycoplasma Detection Kit (Lonza LT07-318).
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bioRxiv - Biochemistry 2019Quote: ... Sf21 cells (2 × 106 cells mL−1) were infected with the P3 viral stock in Insect-XPRESS protein-free medium (Lonza). The infected insect cells were grown at 27 °C with shaking for 66 hours ...
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bioRxiv - Microbiology 2020Quote: ... major promastigotes expressing Cas9 and T7 RNA polymerase proteins [30] were co-transfected with donor DNA and sgRNA templates using V-033 program of Amaxa Nucleofector System (Lonza). Transfected parasites were selected with 50 μg/ml neomycin and cloned in semi-solid culture medium ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with pcDNA3 HA eIF4GI plasmid or control green fluorescent protein (GFP)-expressing plasmid (pMAX-GFP plasmid, Lonza). pcDNA3 HA eIF4GI (1–1599 ...
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bioRxiv - Bioengineering 2021Quote: Primary human umbilical vein endothelial cells (HUVECs) and red fluorescent protein-expressing HUVECs (RFP-HUVECs) were maintained in EMG-2 medium (complete EGM-2 BulletKitTM, Lonza). We obtained human umbilical cords from Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine ...
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bioRxiv - Cell Biology 2020Quote: ... The purity of CyaA batches is higher than 90% as judged by SDS PAGE analysis and contained less than 1 EU of LPS/μg of protein as determined by a standard LAL assay (Lonza). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... Western blots were conducted by running equivalent amounts of protein on 4-20% Tris-Glycine SDS/polyacrylamide gradient gels (Lonza) after reduction with 2-mercaptoethanol and heating to 95°C for 5m ...
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bioRxiv - Immunology 2021Quote: ... for RNP formation 180 pmol crRNA-tracrRNA duplex was mixed with 60 pmol Cas9 protein in Nucleofection Buffer P3 (Lonza) and incubated for at least 10 min at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... This cocktail was provided to the cells together with a ribonucleoprotein complex consisting of crRNA, tracrRNA and Cas9 protein (IDT, refer to “Oligonucleotides” for crRNA sequence) via nucleofection using a 4D nucleofector (Lonza). Next ...
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bioRxiv - Molecular Biology 2023Quote: ... complexes for each peak to be deleted were prepared individually by mixing 120 pmol of sgRNA with 20 pmol of Cas9 protein (QB3 MacroLab, University of California, Berkeley) in 5 ul of P3 primary cell nucleofection buffer (Lonza) and incubated at room temperature for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5-2.0 x 105 cells were electroporated with 200 pmol of protein in 20 µl cuvettes using a 4D nucleofector device (Lonza) according to the manufacturer’s recommendations ...
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bioRxiv - Physiology 2023Quote: ... 2 μl of KCNQ2/3 cDNA plasmid (0.9 g/l) and 1 μl of pmax green fluorescent protein vector (0.5 g/l) (Lonza, Cologne, Germany) were diluted in 125 μl of Opti-MEM medium (Life Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... Sf21 cells seeded at 500,000 cells/mL were transfected with recombinant bacmid DNA to generate the initial baculovirus (P1) in Insect-XPRESSTM Protein-free Insect Cell Medium (Lonza). The P1 virus was amplified two times (P2 and P3 ...
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bioRxiv - Immunology 2024Quote: ... The reaction contained 400 pmol of Cas9 protein and 1 × 107 of the transduced NK cells in 100 µL of P3 solution (Lonza). Immediately after electroporation ...
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bioRxiv - Pathology 2020Quote: ... The purity of the final recombinant proteins was determined to be more than 99% by sodium dodecyl sulfate and polyacrylamide gel (SDS-PAGE) with an endotoxin concentration lower than 2 units/mg protein measured by Limulus Amebocyte Lysate PYROGENT™ 125 Plus (Lonza). In previous experiments we demonstrated that these recombinant proteins were functionally devoid of significant endotoxin contamination ...
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bioRxiv - Immunology 2020Quote: ... Six micrograms of plasmid DNA encoding the cDNA of the desired proteins was used for a single transfection reaction using program T-019 on the Amaxa Nucleofector II (Lonza, MD).
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bioRxiv - Neuroscience 2023Quote: ... TG trigeminal ganglion neurons were transfected with 3 µg of NaV1.7-PEPCy3 plasmid and 0.75 µg green fluorescent protein (GFP) using the rat neuron 4D-Nucleofector solution (P3 Primary Cell Solution, program DR 114; Lonza Biosciences). Neurons were plated on round bottom glass dishes (cat ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines were periodically tested for mycoplasma using the EZ-PCR™ Mycoplasma Detection Kit (Biological Industries, Cromwell, CT, USA) and the MycoAlert™ Mycoplasma Detection Kit (Lonza, Basal, Switzerland) and were confirmed to be mycoplasma free ...
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bioRxiv - Cancer Biology 2021Quote: ... using buffer R (Amaxa Nucleofector Kit R VCA-1003; Lonza) and program R-001 ...
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bioRxiv - Cell Biology 2020Quote: ... siRNA delivery was performed using either Nucleofector Kit V (Lonza) or Neon transfection system (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... contamination with the MycoAlert Mycoplasma Detection kit (Lonza LT-07). Cell lines were not authenticated.
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bioRxiv - Neuroscience 2020Quote: The adenylate kinase assay (ToxiLightTM bioassay kit LT07-217 Lonza) to assess toxicity was performed following the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 days after nucleofection with the AMAXA Nucleofector Kit (Lonza), we applied puromycin selection until we observed the appearance of green colonies ...
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bioRxiv - Cell Biology 2019Quote: ... MCF 10A cells were cultured in the MEGM kit (Lonza) at 37°C with 5% CO2 ...
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bioRxiv - Bioengineering 2020Quote: ... with a 20 μl P3 solution kit (Lonza, #V4XP-3032) with 600k PGP1 WT cells in each 20 μl reaction well as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Mycoplasma contamination was tested using MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Immunology 2021Quote: ... Cells were transfected using the Amaxa HUVEC Nucleofector kit (Lonza) according to the manufacturer’s protocol (2-5 µg plasmid DNA ...