Labshake search
Citations for Lonza :
51 - 100 of 284 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... proteins were separated by SDS-PAGE using 14% Prosieve (Lonza) polyacrylamide gels and electroblotted to Immobilon-P membranes (Millipore) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... with Cas9 ribonucleic protein (RNP) complexes in SF buffer (Lonza), using the pulse codes CM-150 or CV-104 ...
-
bioRxiv - Microbiology 2020Quote: ... DNA yield and quality was assessed by electrophoresis using a 0.75% agarose gel containing the DNA stain GelStar (Lonza, Rockland, ME, USA) in 1X Tris Borate EDTA (TBE ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA was imaged by staining with SYBR green (LONZA). All visual bands as well as the bands at the same locations in the other lanes were excised from the gel ...
-
bioRxiv - Cell Biology 2023Quote: ... Neurons were transfected with plasmid DNA using Nucleofector (Lonza) before plating as described (Minegishi et al. ...
-
bioRxiv - Cancer Biology 2020Quote: ... consisting of 100 pmol of chemically modified sgRNA (Synthego) and 35 pmol of Cas9 protein (St. Jude Protein Production Core) via nucleofection (Lonza, 4D-Nucleofector™ X-unit) using solution P3 and program CA-137 in small cuvettes according to the manufacturers recommended protocol ...
-
bioRxiv - Immunology 2021Quote: ... the final saponin adjuvant was characterized by Limus Amebocyte Lystae assay (QCL-1000, Lonza) for low endotoxin levels ...
-
bioRxiv - Immunology 2021Quote: Endotoxin level in the AF was determined by Limulus amebocyte lysate assay (LAL; Lonza) according to the test procedure recommended by the manufacturer’s instructions.
-
bioRxiv - Bioengineering 2023Quote: The PyroGene Recombinant Factor C Endpoint Fluorescent Assay (Lonza, Walkersville, MD, cat # 50-658U) was performed as recommended ...
-
bioRxiv - Cancer Biology 2023Quote: ... The cells were regularly tested for Mycoplasma by either MycoAlert assay (Lonza, #LT07-418) or MycoStrip (InvivoGen ...
-
bioRxiv - Molecular Biology 2023Quote: ... Intracellular lipid accumulation was quantified by the Adipored Adipogenesis assay reagent (Lonza, Basel, Switzerland). Briefly ...
-
bioRxiv - Microbiology 2020Quote: ... the medium was removed and replaced by Ultradoma Protein-Free (LONZA). The cultured medium was harvested and replenished on the second ...
-
bioRxiv - Genetics 2022Quote: ... membranes were stained with SYPRO-Ruby Protein Gel Stain (Lonza Rockland) to confirm equal loading ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cell lines were routinely tested and verified to be free of mycoplasma (MycoAlert Assay, Lonza). All lines were maintained in RPMI 1640 media (Hyclone or Gibco ...
-
bioRxiv - Cell Biology 2020Quote: ... penicillin and streptomycin and were routinely checked for mycoplasma contamination (LT07-518 Mycoalert assay, Lonza).
-
bioRxiv - Bioengineering 2021Quote: ... The cells tested negative for mycoplasma contamination in the MycoAlert assay (Lonza, Walkersville, MD, USA). This cell line was chosen because of its extensive prior use and validation for lentivirus production(Campeau et al. ...
-
bioRxiv - Bioengineering 2020Quote: ... Endotoxin levels were evaluated by the Limulus amebocyte lysate assay (Endotoxin Testing Services, Lonza Europe), and the amount expressed was negligible ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cell lines were routinely tested and verified to be free of mycoplasma (MycoAlert Assay, Lonza). All lines were maintained in RPMI 1640 media (Hyclone or Gibco ...
-
bioRxiv - Microbiology 2022Quote: ... Cell lines were confirmed to be mycoplasma-negative by MycoAlert PLUS Assay (Lonza #LT07-710) performed by the Koch Institute High Throughput Sciences Facility (Cambridge ...
-
bioRxiv - Developmental Biology 2020Quote: ... The cell lines were routinely checked for mycoplasma contamination using LT07-518 Mycoalert assay (Lonza). For the immunolabeling experiment HeLa cells were transfected with plasmids using FuGENE 6 (Promega) ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were routinely tested for Mycoplasma contamination by luminescence assay (Lonza MycoAlertTM PLUS, LT07-703). For information on cell lines used see Table 1.
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were confirmed to be mycoplasma-negative using the MycoAlert Mycoplasma detection assay (Lonza, LT07218) and maintained in a humidified incubator at 37°C with 5% CO2.
-
bioRxiv - Neuroscience 2020Quote: ... we used 2.2 % agarose gel electrophoresis (FlashGel DNA System, Lonza) and Bioanalyzer High-Sensitivity DNA Assay (Supplementary Figure 2) ...
-
bioRxiv - Systems Biology 2020Quote: ... Sheared DNA was run on a 1% agarose gel (Lonza) to verify that sheared DNA was between 100-1000 bp ...
-
bioRxiv - Cell Biology 2023Quote: ... S2Xpress cells were cultured in Insect-XPRESSTM protein-free medium (LONZA, Belgium) supplemented with 100 U/ml penicillin and 100 mg/ml streptomycin (GIBCO) ...
-
bioRxiv - Immunology 2023Quote: ... Protein expression was induced with 5µM CdCl2 in Insect-XPRESS medium (Lonza) for 5-7 days and supernatants were concentrated and purified using strep-tacting affinity chromatography columns in an AKTA FPLC system ...
-
bioRxiv - Cell Biology 2021Quote: In vitro transendothelial migration (transwell) assay was performed using human umbilical vein endothelial cells (HUVECs; Lonza) grown at 37°C ...
-
bioRxiv - Neuroscience 2022Quote: ... and tested for endotoxin in duplicate using a Kinetic-QCL™ LAL Assay (Lonza, 50-650U) according to manufacturer’s instructions and including a third ‘spiked’ sample from each tissue (5EU/mL endotoxin added ...
-
bioRxiv - Immunology 2020Quote: ... Antibodies which contained endotoxin levels above 3 EU/ml (Kinetic-QCL Kinetic Chromogenic LAL Assay, Lonza) were depleted of endotoxin with the ToxinEraser Endotoxin Removal kit (GenScript ...
-
bioRxiv - Cell Biology 2022Quote: Cell viability was assessed by measuring adenylate kinase (AK) levels using ToxiLight™ assay kit (Lonza). Briefly ...
-
bioRxiv - Genomics 2022Quote: ... and were monitored periodically by the MycoAlert Mycoplasma detection assay (Lonza, cat# LT07-118, NJ, USA) for mycoplasma ...
-
bioRxiv - Immunology 2022Quote: ... Absence of endotoxins (< 1EU/mL) was confirmed using the PyroGene™ Recombinant Factor C Assay (Lonza) according to manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: ... Plasma LPS levels were quantitated with the QCL-1000 Endpoint Chromogenic LAL Assay (Lonza, Basel, Switzerland). Plasma UA levels were determined by the Vistro DT60 II Analyzer at the University of Massachusetts Medical School MMPC (National Mouse Metabolic Phenotyping Center).
-
bioRxiv - Genetics 2022Quote: ... Routine testing for Mycoplasma was performed using a luminescence-based mycoplasma detection assay (Lonza #LT07-705).
-
bioRxiv - Genetics 2023Quote: ... SN4741 cells were confirmed to be mycoplasma free using a MycoAlert® Mycoplasma Detection Assay (Lonza) and were maintained in high glucose Dulbecco’s Modified Eagle Medium (DMEM ...
-
bioRxiv - Immunology 2020Quote: ... both grown in Insect-XPRESS Protein-free Insect Cell Medium (Lonza, BE12-730Q) supplemented with L-glutamine (to 1%) ...
-
bioRxiv - Microbiology 2021Quote: Leukocyte transmigration assay was carried out by addition of 5×106 cells/mL hPBMC (#CC-2702 Lonza) to DENV2-infected HUVEC monolayers at 24 hpi following different treatments ...
-
bioRxiv - Bioengineering 2022Quote: ... termed assay medium (EBM-2 medium containing hFGF, hydrocortisone, GA-1000, 2% FBS, all from Lonza Biosciences). Cells were allowed to settle for 2 hours ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were checked for mycoplasma at the point of cryo-storage using a luminescence-based assay (Lonza).
-
bioRxiv - Immunology 2022Quote: ... 2003) and were free of endotoxin contamination as judged by the QCLTM Kinetic Chromogenic LAL Assay (Lonza). The ADP-heptoses ...
-
bioRxiv - Microbiology 2022Quote: ... All cells were routinely tested and confirmed to be free of mycoplasma (MycoAlert™ PLUS Assay, Lonza).
-
bioRxiv - Cell Biology 2023Quote: Adipocyte differentiation was evaluated by staining cellular lipid droplets with the AdipoRed assay reagent (Lonza, Basel, Switzerland). The fluorescence intensity (excitation/emission = 485/535 nm ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were verified as negative for mycoplasma by testing with the MycoAlert Mycoplasma Detection Assay kit (Lonza) per the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2021Quote: ... phosphodiester linkages in the DNA backbone were cleaved by DNAse I (Lonza).
-
bioRxiv - Developmental Biology 2019Quote: ... The DNA constructs were introduced into the cells with Amaxa Nucleofector (Lonza).
-
bioRxiv - Molecular Biology 2024Quote: ... DNA samples were loaded on 3% NusieveTM GTGTM Agarose gel (Lonza, 50081), and electrophoresis was run at 100V in 1X TBE buffer for ∼50 min ...
-
bioRxiv - Cell Biology 2019Quote: ... Endotoxin was measured with the Limulus Amoebocyte Lysate (LAL) chromogenic end-point assay (Lonza®, Allendale, NJ, USA).
-
bioRxiv - Cancer Biology 2021Quote: ... fixed after 12 days and stained according to the OsteoImage™ Mineralisation Assay Lonza kit (PA-1503, Lonza) protocol ...
-
bioRxiv - Bioengineering 2023Quote: PDLSCs mineralization on the scaffolds after 21 days of osteogenic differentiation was assessed using OsteoImage Mineralization Assay (Lonza). This assay’s staining reagent binds specifically to the hydroxyapatite portion of bonelike nodules deposited by cells ...
-
bioRxiv - Microbiology 2023Quote: ... Cell lines were confirmed to be mycoplasma-negative in a MycoAlert PLUS assay (Lonza catalog number LT07-710) performed by the Koch Institute High-Throughput Sciences Facility (Cambridge ...