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Citations for Lonza :
1 - 50 of 102 citations for Protein Arginine Methyltransferase 8 PRMT8 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... the total protein was size-separated on an 8% polyacrylamide gel and stained with SYPRO Ruby (Lonza). When the contents of the protein preparation were analyzed by western blotting ...
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bioRxiv - Microbiology 2022Quote: ... pH 8 (Lonza), stained with SyberGreen (Sigma Aldrich) ...
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bioRxiv - Systems Biology 2019Quote: ... supplemented with 8 mM L-Glutamine (Lonza) and 1 μL per mL anti-clumping agent (Life Technologies) ...
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bioRxiv - Bioengineering 2019Quote: ... and 8 mM L-glutamine (Lonza, Basel, Switzerland). Fed-batch cultivation was performed in a DASGIP Mini bioreactor (Eppendorf ...
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bioRxiv - Microbiology 2021Quote: An 8% SeaPlaque GTG Agarose (Lonza, Basel, Switzerland) solution in Ultra Pure Water (Genesee Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies were validated with Western blot analysis using protein from Neuronal Human Astrocytes (Lonza, CC-2565) as previously reported in Knight and Serrano (2017a).
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 8 mM ultraglutamine (Lonza, #BE17-605E/U1), 1 X HT supplement (Gibco ...
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bioRxiv - Bioengineering 2020Quote: ... HUVEC cells were cultured to <8 passages in EBM-2 (Lonza) supplemented with EGM-2 Single Quots (Lonza) ...
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bioRxiv - Biophysics 2021Quote: ... in passages 4-8 were cultured in EGM2-MV medium (Lonza) at 37°C in a humidified atmosphere of 95% air and 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... 8 and 14 of culture with 70 µL of BEGM** (Lonza). On day 21 ...
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bioRxiv - Cell Biology 2022Quote: ... in passages 4-8 were cultured in EGM2-MV medium (Lonza) at 37°C in a humidified atmosphere of 95% air and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... in passages 4-8 were cultured in EGM2-MV medium (Lonza).
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bioRxiv - Cell Biology 2022Quote: ... 8 and 14 post printing according to the manufacturer’s protocol (Lonza, Basal, Switzerland). See Method supplement for details
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bioRxiv - Microbiology 2023Quote: ... 8 µg RNA per lane was first resolved on a 1.2% agarose gel (Lonza) and transferred for 20 hours to a nylon membrane by capillary action ...
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bioRxiv - Immunology 2022Quote: ... 1 × 106 cells were transfected with 8 μg ScaI-linearized plasmid using the Amaxa Nucleofector (Lonza) SF kit on program DS-113 ...
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bioRxiv - Cell Biology 2023Quote: Drosophila ovaries (5-8 pairs per sample) were dissected into room temperature Grace’s insect medium (Lonza). Ovaries were fixed for 10 min using 4% paraformaldehyde diluted in Grace’s medium ...
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bioRxiv - Microbiology 2020Quote: ... Integrity and purify of SyAMs were assessed by SDS-PAGE analysis using 8-16 % gradient gel (Lonza).
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bioRxiv - Neuroscience 2022Quote: ... 8 wells were transfected using Amaxa nucleofection with P3 Primary Cell 4D-Nucleofector 16-well Strips (Lonza). Each well contained a single-cell suspension of 1.6 × 105 cells in 20 µl of Primary Cell P3 buffer with supplement (Lonza ...
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bioRxiv - Bioengineering 2021Quote: ... (8 x 102 cells for H460) in ultra-low adherent round-bottom 96-well plates (Lonza, Australia) for a total of 72 hours ...
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bioRxiv - Bioengineering 2023Quote: ... in passages 5-8 were cultured at 37°C and 5% CO2 in EGM2 cell medium (Lonza). Cells were detached from the adhering surface using TrypLE (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... using antibiotic-free endothelial media and were lifted for seeding at passage 5-8 (Lonza, Walkersville, MD). After lifting ...
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bioRxiv - Genomics 2021Quote: ... 1.6×105 cells were suspended in P3 Primary Cell Solution with 50pmol Cas9 protein (St. Jude Protein Production Core) and 150pmol sgRNA (Synthego) with pMaxGFP plasmid at 200ng/ul (Lonza) in a total volume of 20ul with program DS130 ...
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bioRxiv - Genomics 2021Quote: ... and 50 pmole of spCas9 protein (St. Jude Protein Production Core) with pMaxGFP plasmid as a transfection control at 200ng/ul (Lonza) using the 4D-Nucleofector™ X-unit (Lonza ...
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bioRxiv - Molecular Biology 2019Quote: ... were obtained from Lonza (CC-2581) and maintained for no more than 8 passages in Smooth Muscle Cell Growth Media (SM-GM2, Lonza) supplemented with 5% FBS and 1X Penicillin/Streptomycin (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1×106 EBdCas9 cells were transfected with the above RNP complex and 100μM of ssDNA (HDR)(Sup Table 8) using AMAXA electroporation kit (LONZA) and plated in TeSR media (supplemented with 5μM ROCKi) ...
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bioRxiv - Immunology 2021Quote: ... washed in PBS by centrifugation for 5 min at 1300 rpm at 4°C and resuspended at 4.0 × 106 cells/ml in complete medium (IMDM 2 mM glutamax I supplemented with 8% heat-inactivated FCS (Lonza), 2% heat-inactivated chicken serum ...
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bioRxiv - Bioengineering 2020Quote: ... 8 volumes of collagen I gel solution (10mg/ml) were mixed with 1 volume of 10X EMEM (Lonza: 12-684F) and 1 volume of 10X Reconstruction buffer(Anacker and Moody 2012 ...
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bioRxiv - Cell Biology 2020Quote: ... and 8 × 105 cells were electroporated using 5 mg of total DNA (Ratio 1:1) and the Amaxa Nucleofector kit (Lonza) as described (Ran et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Kp27MYCER and B95-8) and adherent cell lines (HeLa, HEK293T) were grown in RPMI-1640 or DMEM basal media (Lonza) respectively supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... were isolated from 8 to 16-week-old Rptorfl/fl or Tsc2fl/fl mice and maintained in EGM-2 medium (Lonza), as previously described71–75 ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow cells were extracted from long bones of C57BL/6J mice 6 to 8 weeks of age as described (Guimbal et al.. 2019) and cultured in α-minimal essential medium (αMEM. Lonza) containing 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... The concentration of IL-2 was increased to 1000 IU/ml on day 7 and the expanded T cells were electroporated with the indicated mRNA at a concentration of 2 pg mRNA/cell on day 8 using 4D Nucleofector™ System (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: We prepared thin agar pad for the samples where 8 µL of semi-cooled 1.5% agarose solution (add low melting temperature NuSieve GTG Agarose (Lonza, Cat# 50081) to 2% galactose media (0.77 g of CSM-URA (Sunrise # 1004-010) ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were separated by SDS-PAGE using 14% Prosieve (Lonza) polyacrylamide gels and electroblotted to Immobilon-P membranes (Millipore) ...
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bioRxiv - Synthetic Biology 2023Quote: ... with Cas9 ribonucleic protein (RNP) complexes in SF buffer (Lonza), using the pulse codes CM-150 or CV-104 ...
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bioRxiv - Developmental Biology 2020Quote: ... about 8 × 105 iPSCs were transfected with 5 μg of plasmids with Lonza Human Stem Cell Nucleofector Kit 1 (Lonza, VPH-5012) on a Nucleofector 2b device (Lonza ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then passaged as previously described and 8×105 cells were resuspended in 100 µl of nucleofector solution (Lonza, VPH-5012). Depending on the experiment ...
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bioRxiv - Cancer Biology 2020Quote: ... consisting of 100 pmol of chemically modified sgRNA (Synthego) and 35 pmol of Cas9 protein (St. Jude Protein Production Core) via nucleofection (Lonza, 4D-Nucleofector™ X-unit) using solution P3 and program CA-137 in small cuvettes according to the manufacturers recommended protocol ...
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bioRxiv - Cancer Biology 2019Quote: All tumouroids were fabricated using monomeric Type I rat-tail collagen (First Link, Birmingham, UK) and the RAFT™ protocol pages 8-9 (Lonza, Slough, UK) as previously described21 ...
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bioRxiv - Microbiology 2020Quote: ... the medium was removed and replaced by Ultradoma Protein-Free (LONZA). The cultured medium was harvested and replenished on the second ...
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bioRxiv - Genetics 2022Quote: ... membranes were stained with SYPRO-Ruby Protein Gel Stain (Lonza Rockland) to confirm equal loading ...
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bioRxiv - Immunology 2023Quote: The 3 kbp DNA plasmid encoding green fluorescent protein (GFP) (Lonza) was used to assess transfection efficiency ...
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bioRxiv - Cell Biology 2023Quote: ... S2Xpress cells were cultured in Insect-XPRESSTM protein-free medium (LONZA, Belgium) supplemented with 100 U/ml penicillin and 100 mg/ml streptomycin (GIBCO) ...
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bioRxiv - Immunology 2023Quote: ... Protein expression was induced with 5µM CdCl2 in Insect-XPRESS medium (Lonza) for 5-7 days and supernatants were concentrated and purified using strep-tacting affinity chromatography columns in an AKTA FPLC system ...
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bioRxiv - Immunology 2020Quote: ... both grown in Insect-XPRESS Protein-free Insect Cell Medium (Lonza, BE12-730Q) supplemented with L-glutamine (to 1%) ...
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bioRxiv - Microbiology 2020Quote: ... cultured in Insect-XPRESS Protein-free Insect Cell Medium supplemented with L-glutamine (Lonza). A Sf21 cell culture of ∼1.8 × 106 cells/ml was inoculated with the third passage stock of virus and incubated for three days at 28 °C ...
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bioRxiv - Immunology 2022Quote: ... the protein extraction was done with IP buffer (50 mM Hepes pH 7,5 (Lonza), 150 mM NaCl (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained with surface antibodies in PBS (Lonza) + 2% FCS ...
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bioRxiv - Immunology 2021Quote: ... antibodies in serum-free X-Vivo 20 medium (Lonza), in the absence (Th0 ...
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bioRxiv - Immunology 2022Quote: Cells were stained with surface antibodies in PBS (Lonza) + 2% FCS ...