Labshake search
Citations for Lonza :
1 - 50 of 142 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... the protein extraction was done with IP buffer (50 mM Hepes pH 7,5 (Lonza), 150 mM NaCl (Sigma-Aldrich) ...
-
bioRxiv - Physiology 2023Quote: ... 2 μl of KCNQ2/3 cDNA plasmid (0.9 g/l) and 1 μl of pmax green fluorescent protein vector (0.5 g/l) (Lonza, Cologne, Germany) were diluted in 125 μl of Opti-MEM medium (Life Technologies) ...
-
bioRxiv - Bioengineering 2021Quote: ... beads were removed using a magnetic holder and washed with PBS once prior to resuspension in P3 electroporation buffer (Lonza). Samples were electroporated using the FI-115 program on the 4D-nucleofector (Lonza ...
-
bioRxiv - Neuroscience 2023Quote: ... iPS cells were passaged every 3-4 days with Accutase (Lonza, Basel, Switzerland) and seeded on new plates with density 1:5 – 1:10 in iPS medium with ROCK inhibitor Y-27632 (Sigma-Aldrich) ...
-
bioRxiv - Developmental Biology 2020Quote: ... iPS-macrophages were cultured in macrophage medium composed of X-VIVO™15 (Lonza), GlutaMax (Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... The forth iPS cell line was reprogrammed from human dermal fibroblasts (CC-2511, Lonza) of a 45 years old healthy donor in our lab using Cyto TuneTM iPS 2.0 sendai reprogramming kit (A16517 ...
-
bioRxiv - Neuroscience 2023Quote: ... TG trigeminal ganglion neurons were transfected with 3 µg of NaV1.7-PEPCy3 plasmid and 0.75 µg green fluorescent protein (GFP) using the rat neuron 4D-Nucleofector solution (P3 Primary Cell Solution, program DR 114; Lonza Biosciences). Neurons were plated on round bottom glass dishes (cat ...
-
bioRxiv - Molecular Biology 2024Quote: ... 0.5 g of agarose powder (Lonza) were mixed in 45 mL of Milli-Q H2O in the flask by swirling but thoroughly ...
-
bioRxiv - Cell Biology 2020Quote: ... iPS cells were then nucleofected with the Amaxa Nucleofection system using the following program (P3, CA-137) (Lonza). The cells were allowed to recover for 36-48 hours at high density in the presence of ROCK inhibitor and then isolated by FACS for GFPhi cells ...
-
bioRxiv - Pathology 2023Quote: ... was maintained in growth media comprised of high glucose (4.5 g/L) and L-glutamine (0.6 g/L) Dulbecco’s Modified Eagle’s Media (DMEM) (#BE12-741F, Lonza), 10% foetal bovine serum (FBS ...
-
bioRxiv - Pathology 2023Quote: Differentiated C2C12 myotubes were incubated in serum-free glucose (4.5 g/L) and L-glutamine (0.6 g/L) DMEM (#BE12-741F, Lonza) with 1% Penicillin-Streptomycin (10,000 U/ml ...
-
bioRxiv - Pathology 2023Quote: ... C2C12 myoblasts were differentiated into myotubes in a differentiation media comprised of high glucose (4.5 g/L) and L-glutamine (0.6 g/L) DMEM (#BE12-741F, Lonza), 2% horse serum (HS ...
-
bioRxiv - Cell Biology 2021Quote: ... Patients-derived iPS cells were electroporated with plasmids and single stranded DNA donor oligos using Human Stem Cell NucleofectorTM Kit 1 (Lonza). After 36 hours ...
-
bioRxiv - Neuroscience 2022Quote: ... This plasmid was nucleofected into the NGN2 iPS line (obtained as described above) using a nucleofection kit (Human Stem Cell Nucleofector Kit 1, VPH-5012, Lonza) containing 4 μg of RFP PB construct and 1 μg of dual helper plasmid ...
-
bioRxiv - Cell Biology 2020Quote: ... cell monolayers were washed twice in sterile D-PBS and cells were detached with Trypsin 0.5 g/L EDTA 0.2 g/L (Lonza, # BE17-161E) for 5 minutes ...
-
bioRxiv - Physiology 2022Quote: ... (5 g/L trypsin and 2 g/L EDTA diluted 1 in 5 in calcium- and magnesium-free PBS) (Lonza, UK).
-
bioRxiv - Biophysics 2020Quote: ... high glucose (4.5 g/l) with L-glutamine (BE12-604F, Lonza) supplemented with 10% (v/v ...
-
bioRxiv - Biophysics 2023Quote: ... high glucose (4.5 g/L) with L-glutamine (BE12-604F, Lonza) supplemented with 10% (v/v ...
-
bioRxiv - Biophysics 2023Quote: ... high glucose (4.5 g/l) with L-glutamine (BE12-604F, Lonza) supplemented with 10% (v/v ...
-
bioRxiv - Neuroscience 2021Quote: ... were cultured in DMEM with 4.5 g/L glucose & L-Glutamine (Lonza), 10% FBS (Sigma ...
-
bioRxiv - Molecular Biology 2020Quote: 3T3-L1 cells were cultured in 4.5 g/L glucose DMEM (Lonza) with 10% FBS ...
-
bioRxiv - Neuroscience 2024Quote: ... were cultured in DMEM with 4.5 g/L glucose & L-Glutamine (Lonza), 10% FBS (Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were cultured in 4.5 g/L glucose containing DMEM (BE12-614F, LONZA), supplemented with 10% fetal bovine serum (10500-064 ...
-
bioRxiv - Immunology 2021Quote: Peripheral blood mononuclear cells (PBMCs) were isolated using Histopaque density 1.077 g/mL (Lonza). CD14+ cells were sorted with the MACS system (Miltenyi Biotec) ...
-
bioRxiv - Microbiology 2021Quote: ... bone marrow (BM) or G-CSF mPB-CD34+ cells were directly purchased from Lonza or Hemacare ...
-
bioRxiv - Molecular Biology 2024Quote: The agarose solution was prepared by dissolving 2.5 g of Seakem LE agarose (Lonza) in 225 ml of Milli-Q water in a microwave ...
-
bioRxiv - Molecular Biology 2024Quote: The agarose solution was prepared by dissolving 2.5 g of Seakem LE agarose (Lonza) in 225 mL of Milli-Q water by microwave ...
-
bioRxiv - Developmental Biology 2020Quote: ... at a ratio of 400 cells per bead in EGM-2MV medium (Lonza) for 4 hours at 37°C with shaking every 20 minutes ...
-
bioRxiv - Neuroscience 2020Quote: ... Mononuclear cells were collected by density centrifugation using lymphocyte separation media (1.077 g/mL, Lonza) as previously described 96 ...
-
bioRxiv - Genomics 2021Quote: ... 1.6×105 cells were suspended in P3 Primary Cell Solution with 50pmol Cas9 protein (St. Jude Protein Production Core) and 150pmol sgRNA (Synthego) with pMaxGFP plasmid at 200ng/ul (Lonza) in a total volume of 20ul with program DS130 ...
-
bioRxiv - Genomics 2021Quote: ... and 50 pmole of spCas9 protein (St. Jude Protein Production Core) with pMaxGFP plasmid as a transfection control at 200ng/ul (Lonza) using the 4D-Nucleofector™ X-unit (Lonza ...
-
bioRxiv - Microbiology 2019Quote: ... BHK-T7 cells were grown in Glasgow complete medium [Glasgow minimum essential medium (G-MEM) (Lonza), 10% tryptone-peptide broth (Gibco) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were differentiated into bone marrow derived macrophages (BMDMs) in 4.5 g/L glucose DMEM (Lonza) with 20% fetal bovine serum (FBS) ...
-
bioRxiv - Cell Biology 2022Quote: ... Caco2 cells were cultured in DMEM 4.5 g/L Glucose with UltraGlutamine (Lonza: H3BE12-604F/U1) medium containing 20% fetal calf serum (Gibco ...
-
bioRxiv - Cell Biology 2022Quote: ... containing 4.5 g/L glucose and supplemented with L-glutamine with sodium pyruvate (Lonza, Basel, Switzerland), 10% fetal bovine serum (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2023Quote: ... Virus was concentrated using ultracentrifugation (95000 g for 3 hr) and suspended using OPTI-MEM (Lonza). Viral transfections assays were performed by plating target 293T (ACE2+ve ...
-
bioRxiv - Cell Biology 2023Quote: ... Human SH-SY5Y dopaminergic neuroblastoma cells were cultured in Dulbecco’s modified Eagle’s medium:F-12 (DMEM:F-12; 1:1) containing high glucose (3.151 g/L) (Lonza), supplemented with 10% (v/v ...
-
bioRxiv - Genomics 2019Quote: ... TetO-CDC6 mESCs were cultured on 0.1% gelatin-coated plates in Dulbecco’s modified Eagle’s medium (DMEM) with Ultraglutamine 1 and 4.5 g/L glucose (Lonza) supplemented with 15% FBS (Sigma) ...
-
bioRxiv - Cell Biology 2021Quote: ... were maintained in Dulbecco’s modified Eagle’s medium with 4.5 g/l glucose with L-Glutamin (DMEM; Lonza), supplemented with 8% fetal calf serum (FBS ...
-
bioRxiv - Immunology 2019Quote: ... T cells were centrifuged for 10 min at 90x g and resuspended in electroporation buffer P3 (Lonza) at a concentration of 750,000 cells/ 20 μL ...
-
bioRxiv - Genetics 2019Quote: ... were cultured in growth medium consisting of: DMEM containing L-Glutamine and 4.5 g/L Glucose (Lonza), with the addition of 10 % Foetal Bovine Serum (FBS) ...
-
bioRxiv - Molecular Biology 2022Quote: SH-SY5Y and HEK293T cells were grown in Dulbecco’s modified Eagle’s medium containing L-glutamine and 4.5 g/L glucose (DMEM; Lonza) and supplemented with 10% fetal calf serum (Gibco ...
-
bioRxiv - Cell Biology 2024Quote: ... The supernatant was centrifuged at 300 g for 5 min and cells resuspended in X-VIVO152 (Lonza), OXM4 or HPLM (A4899101)3 supplemented with 100 ng/ml hM-CSF and plated at 4×106 cells per 10 cm petri dish to differentiate over 7 days ...
-
bioRxiv - Pathology 2023Quote: ... Cells were stimulated with anti-CD3/CD28 beads (MiltenyiBiotec) for two days in cRF10: RPMI (Lonza) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2019Quote: DIvA cells were cultured at 37 °C in DMEM (Dulbecco’s Modified Eagle Medium 4.5 g/l glucose, supplemented with L-pyruvate; Lonza,) supplemented with 10 % foetal bovine serum (Lonza) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Dulbecco’s modified eagle media (DMEM) with 4.5 g/L glucose and L-glutamine was from Lonza (Basel, Switzerland). Minimal Essential Media (MEM ...
-
bioRxiv - Biochemistry 2021Quote: ... proteins were separated by SDS-PAGE using 14% Prosieve (Lonza) polyacrylamide gels and electroblotted to Immobilon-P membranes (Millipore) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... with Cas9 ribonucleic protein (RNP) complexes in SF buffer (Lonza), using the pulse codes CM-150 or CV-104 ...
-
bioRxiv - Cancer Biology 2020Quote: ... consisting of 100 pmol of chemically modified sgRNA (Synthego) and 35 pmol of Cas9 protein (St. Jude Protein Production Core) via nucleofection (Lonza, 4D-Nucleofector™ X-unit) using solution P3 and program CA-137 in small cuvettes according to the manufacturers recommended protocol ...
-
bioRxiv - Neuroscience 2019Quote: ... 8 × 105 cells were co-transfected with 3 µg plasmid-sgRNA construct and ssODN (0.6 µM final concentration) with Amaxa Human Stem Cell Nucleofector Kit 1 (Lonza) and program A-023 for the Amaxa Nucleofector Device II ...