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Citations for Lonza :
1 - 50 of 80 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... the porcine fibroblasts were cultured at 37°C in DMEM (#BE12-604F, Lonza Biowhittaker) supplemented with 15% heat-inactivated fetal calf serum ...
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bioRxiv - Immunology 2022Quote: ... Lysate were pellet (500 g 8min) and red blood cells were lysate with 500 µl of ACK lysing buffer (Lonza) for 5min ...
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bioRxiv - Molecular Biology 2020Quote: ... Limulus Amebocyte Lysate Assay (Lonza, Walkersville, MD, USA), 10% neutral buffered formalin (CellPath Ltd ...
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bioRxiv - Bioengineering 2023Quote: ... BOECs were generated from peripheral porcine blood as previously described.13 BOECs were maintained in endothelial growth medium-2 (EGM-2) (#CC-3162, Lonza, Portsmouth, NH) supplemented with 10% fetal bovine serum (#F08BB22A1 ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were loaded on 12% Prosieve 50 gels (Lonza) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were loaded on 10% ProSieve gel (LONZA, LON50618), then subjected to electrophoresis ...
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bioRxiv - Microbiology 2020Quote: ... LPS level was determined using QCL-1000 Limulus amebocyte lysate system (Lonza) and found to be >5 pg/ μg of rfhSP-D.
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bioRxiv - Neuroscience 2024Quote: ... Dissected Drosophila tissues were washed with PBS (Lonza) and fixed with 4% paraformaldehyde (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... the endotoxin levels were measured via the QCL-1000 Limulus amoebocyte lysate system (Lonza) and found to be <5 pg/μg of rfhSP-D ...
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bioRxiv - Immunology 2021Quote: Endotoxin level in the AF was determined by Limulus amebocyte lysate assay (LAL; Lonza) according to the test procedure recommended by the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... the tissue was trypsinized (0.05% Trypsin-EDTA (Lonza, HiMedia) at 37°C for 15-20 minutes and then resuspended in Optimem® (Gibco ...
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bioRxiv - Genomics 2023Quote: ... the tissues were covered with Gel Slick (Lonza #50640)-treated circular coverslips of 18 mm diameter (Ted Pella ...
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bioRxiv - Bioengineering 2020Quote: ... Endotoxin levels were evaluated by the Limulus amebocyte lysate assay (Endotoxin Testing Services, Lonza Europe), and the amount expressed was negligible ...
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bioRxiv - Biochemistry 2020Quote: ... Tissues were embedded in 6% low-melting point agarose (Lonza) for two minutes on ice ...
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bioRxiv - Neuroscience 2022Quote: ... The dissected tissue was placed in HBSS (Lonza, 10-508F) and weighed ...
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bioRxiv - Cell Biology 2024Quote: ... derived from human dental pulp tissues were purchased from Lonza for use in this study ...
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bioRxiv - Microbiology 2021Quote: ... The levels of endotoxin were measured using the Limulus Amebocyte Lysate (LAL) Kinetic-QCL kit (Lonza). Phage or AAVP preparations containing endotoxin levels < 0.05 EU/mL were used in this study.
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bioRxiv - Physiology 2022Quote: ... Cleared lysates were loaded into wells of the SDS-agarose gels (1% SeaKem Gold Agarose [Lonza, Basel ...
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bioRxiv - Cell Biology 2023Quote: ... kidney tissues were washed on ice in HBSS (Lonza, Walkersville, MD) after removal from DMEM ...
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bioRxiv - Immunology 2023Quote: ... IPF tissue was injected with 2% low melting agarose (50111, Lonza) and then submerged in ice-cold PBS to allow solidification of agarose ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was embedded in 4% low-melting agarose in PBS (SeaPlaque, Lonza), mounted on a vibratome with cold PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Immediately after surgery adipose tissue was place into Medium 199 (M199) (Lonza) with penicillin (100 U/l) ...
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bioRxiv - Cell Biology 2019Quote: ... Endotoxin was measured with the Limulus Amoebocyte Lysate (LAL) chromogenic end-point assay (Lonza®, Allendale, NJ, USA).
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bioRxiv - Immunology 2019Quote: ... Endotoxin concentrations were measured in all protein samples using the Limulus Amebocyte Lysate Kit – QCL-1000 (Lonza, Basel, Switzerland). The rMIC1 ...
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bioRxiv - Immunology 2021Quote: ... and the levels of endotoxins were measured using the Chromogenic Limulus Amebocyte Lysate QCL-1000 Assay (Lonza, Walkersville, MD). Fh12 was considered endotoxin free when endotoxin levels gave similar to background levels (<0.1 EU/ml) ...
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bioRxiv - Microbiology 2023Quote: ... and the absence of endotoxin was confirmed using the Chromogenic Limulus Amebocyte Lysate QCL-1000 Assay (Lonza, Walkersville, MD). nFhGST was concentrated by AMICON Ultra-15 Centrifugal filter unit and adjusted to 1 mg/ml as determined using the Pierce protein bicinchoninic acid (BCA ...
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bioRxiv - Genetics 2019Quote: ... Lysates were resolved by electrophoresis (Mini-PROTEAN TGX Precast Gels, BioRad; or PAGEr EX Gels, Lonza; or home-made gels), proteins transferred onto PVDF membranes (Immobilon P ...
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bioRxiv - Immunology 2020Quote: ... Endotoxin concentrations ranged from 150-175 EU/mL as determined using the limulus amebocyte lysate assay (Lonza, Walkersville, MD, USA). This concentration of ODE has been previously shown to produce optimal experimental effects and is well-tolerated in mice (11 ...
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bioRxiv - Cell Biology 2020Quote: Microspheres were tested for LPS contamination using the Limulus Amebocyte Lysate (LAL) QCL-1000™ kit (Lonza Walkersville, Inc., Olten, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Residual endotoxins in the purified phage preparations were determined by the Limulus Amebocyte Lysate (LAL) Kinetic-QCL Kit (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... The purity of the final recombinant proteins was determined to be more than 99% by sodium dodecyl sulfate and polyacrylamide gel (SDS-PAGE) with an endotoxin concentration lower than 2 units/mg protein measured by Limulus Amebocyte Lysate PYROGENT™ 125 Plus (Lonza). In previous experiments we demonstrated that these recombinant proteins were functionally devoid of significant endotoxin contamination ...
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bioRxiv - Immunology 2019Quote: ... Mucin proteins was prepared as previously described45 and confirmed to be endotoxin free using a Limulus Amebocyte Lysate (LAL) assay kit (Lonza, USA). Full details of mucin protein purification and preparation are shown in supplementary methods ...
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bioRxiv - Immunology 2023Quote: ... acetylated chitin oligomers were suspended in endotoxin free water and tested for endotoxin level by using the limulus amebocyte lysate (LAL) assay (Lonza, CH). Levels below 0.25 EU/ml (<25 pg/ml LPS ...
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bioRxiv - Neuroscience 2020Quote: ... fresh human foetal brain tissue was chopped mechanically and dissociated with Trypsin (LONZA; BE17-161E) 1:5 in growth medium for 5-10 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... after which the tissue left standing at room temperature in 10ml RPMI (Lonza, BE12-702F) supplemented with 5% (v/v ...
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bioRxiv - Immunology 2023Quote: ... The levels of endotoxin in the purified protein samples were assessed using the QCL-1000 Limulus amebocyte lysate system (Lonza, Basel, Switzerland), and it was found that the recombinant proteins had endotoxin levels of ∼ 4 pg/µg ...
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bioRxiv - Immunology 2020Quote: ... Human ventricular cardiac fibroblasts that were harvested from normal adult ventricular tissue were obtained from Lonza, maintained at low passage number (<12) ...
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bioRxiv - Cell Biology 2023Quote: ... Macroscopically healthy tissue was immediately washed with sterile Hank’s balanced salt solution (HBSS; Lonza, Walkersville, MD), and portions were ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Tissues were either commercially available (MucilAir™, Epithelix) or prepared in-house from hAECs (Epithelix, Lonza). Measured tissues originated from six F508del homozygous patients (CF patient #1 ...
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bioRxiv - Pathology 2020Quote: ... The purity of the final recombinant proteins was more than 99% with an endotoxin concentration lower than 2 units/mg protein by Limulus Amebocyte Lysate PYROGENT™ 125 Plus (Lonza; Walkersville, MD).
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bioRxiv - Cell Biology 2021Quote: ... endotoxin-free phosphate buffered saline (PBS) and verified to be free of detectable lipopolysaccharide contamination by Limulus lysate assay (Lonza, Walkersville, Maryland, USA), MCSF (10 ng/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... placed on the tissue culture plates with a small drop of LONZA culture medium (LONZA, Walkersville, MD) supplemented with SmGM-2 or FGM-2 media kit for PAVSMC and PAAF isolation ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were cultured on laminin-coated tissue culture flasks in SKGM-2 medium (Lonza®, Valkersville, MD). Medium was replaced every 3 days ...
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bioRxiv - Immunology 2021Quote: ... were expanded in 75 cm2 tissue culture flasks using human endothelial cell growth medium (Lonza, CC-3202) until 70-80% confluent.
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bioRxiv - Immunology 2021Quote: ... The remaining tissue was minced and digested with stirring for 60 min in complete RPMI (RPMI1640; Lonza; supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Neuroscience 2020Quote: ... Ipsilateral and contralateral brain tissues were placed in cold Hank balanced salt solution (HBSS + Ca/Mg; Lonza) and mechanically dissected through a 100 µm cell strainer ...
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bioRxiv - Microbiology 2020Quote: ... thymus lobes were cut in small pieces and cleaned for blood and connective tissue in RPMI (Lonza) supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were detached from tissue culture flasks using PBS containing 0.25% trypsin (Lonza, Gaithersburg, MD, USA). The isolated endMSCs were characterized by flow cytometry and differentiation assay ...
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bioRxiv - Cancer Biology 2023Quote: Human breast adipose tissue was incubated in mammary epithelial cell growth base medium (MEBM) (Lonza #cc-3151) supplemented with 0.5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2019Quote: ... Minced tissue were put into a 15-mL falcon tube filled with 3mL EBM-2MV (Lonza, Cologne, Germany) supplemented with 0.5% FBS ...