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Citations for Lonza :
51 - 87 of 87 citations for PhotoCol Methacrylated Type I Collagen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... American Type Culture Collection (ATCC)] were grown in Eagle’s minimal essential medium (EMEM; Lonza) supplemented with 8% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2021Quote: HepG2 hepatoma cells were cultured in collagen-coated T75 flasks in minimum essential medium eagle with Earle’s BSS (MEM Eagle EBSS) from Lonza (Walkersville, MD) supplemented with 10% FBS and 4.4 mM sodium pyruvate at 37 °C with 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... and electroporated with program X-01 on the Nucleofector I system (Lonza). Cells were used 48 h after siRNA treatment ...
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bioRxiv - Molecular Biology 2021Quote: ... phosphodiester linkages in the DNA backbone were cleaved by DNAse I (Lonza).
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bioRxiv - Microbiology 2020Quote: ... 0.1 µL of a 100 X stock of SYBR Green I (Lonza), and 500 U Hot Start Taq DNA Polymerase (New England Biolabs) ...
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bioRxiv - Biochemistry 2022Quote: Normal bronchial epithelial cells (BEAS-2B / ATCC-CRL-9609) were cultured in collagen coated flasks using the bronchial epithelial growth medium supplemented with Lonza Bullet Kit (BEGM, Lonza CC-3170), as described by ATCC culture instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Fixed cells were then stained with 2X SYBR® Green I (Lonza, 50512) and parasitemia was determined by flow cytometry using the Guava® EasyCyte™ HT flow cytometer (Luminex).
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bioRxiv - Bioengineering 2021Quote: ... Then cells were cultured in differentiation medium I consisting EBM-2 (Lonza, #CC-3156), 0.1% FBS ...
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bioRxiv - Immunology 2022Quote: ... The nucleofection was then performed by AMAXA Nucleofector I device X-01 program (Lonza). The nucleofected cells were rested in the cuvette at RT for 10min before being transferred out for downstream applications ...
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bioRxiv - Microbiology 2023Quote: ... containing 0.3 μl/ml SYBR Green I (10,000× in DMSO; Lonza, Cat. No. 50513). Plates were incubated in the dark for at least 1 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... 100 µL aliquots (4 × 106 cells) were electroporated with an Amaxa Nucleofector I (Lonza) using the HEK293 pulse sequence ...
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bioRxiv - Molecular Biology 2023Quote: One million Aire wild-type mTEC 3.10 cells were suspended in 100 μL of nucleofector solution (Lonza) containing 20 pmol of spCas9-GFP ...
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bioRxiv - Genomics 2022Quote: ... Wild type K562 cells were transfected with both the donor and guide plasmids by nucleofection (Lonza, program T016). Cells were selected 3 days after transfection with puromycin (2µg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa Myo10 KO1 cells were nucleofected using an Amaxa nucleofection apparatus (Lonza; program I-013) with Piggybac plasmid and either WT Xlone-mScarlet-Myo10 or mutant Xlone-mScarlet-Myo10 ...
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bioRxiv - Bioengineering 2022Quote: NK-92 cell line was acquired from the American Type Culture Collection and maintained in X-VIVO 10 (Lonza) supplemented with 20% FBS and 0.02 mM folic acid (Millipore Sigma ...
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bioRxiv - Molecular Biology 2019Quote: Wild type SV40-immortalized human fibroblasts (MRC5) were cultured in a 1:1 mixture of Ham’s F10 and DMEM (Lonza) supplemented with 1% antibiotics (penicillin and streptomycin ...
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bioRxiv - Molecular Biology 2019Quote: ... and wild-type J1 male mouse embryonic stem cells were grown on 0.1% gelatin-coated dishes in DMEM (Lonza) complemented with 10% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... then the ventricles were dissected and dissociated by serial digestion at 37°C with 0.44 mg/ml (6800 U) Worthington Type II collagenase (supplied by Lonza) and 0.6 mg/ml pancreatin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: Human retinal pigmented epithelium ARPE-19 cells [American Type Culture Collection (ATCC) CRL-2302] were grown in a 1:1 (v/v) mixture of DMEM (Lonza) and Ham’s F12 (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 μg of linearized plasmid containing wild-type or Ku70 3A cDNA was transfected using Amaxa nucleofector solution V (Lonza) and program T-020 ...
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bioRxiv - Biochemistry 2021Quote: ... Transfections of full-length human EGFR (wild-type or noted variants) into Ba/F3 or eHAP cells were performed by electroporation using a Nucleofector 2b device (Lonza) as described previously (Kiyatkin et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Both cell types were cultured and used in TOC devices for up to seven passages in complete growth medium from Lonza (FGM-2 for NHLFs and EGM-2 for ECFC-ECs) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 × 106 wild-type K562 cells were electroporated in 100 μl of Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1 µg of PiggyBac expression vector (PB200A-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells generated by transfection with pcDNA3 plasmid containing wild-type BRCA1 (27) were both grown in 50% RPMI-1640 and 50% Mammary Epithelial Growth Medium (Lonza, Basel, Switzerland). Medium for UWB+ B1 cells was supplemented with 200 μg/ ml G418 S (Merck ...
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bioRxiv - Cell Biology 2024Quote: The NK-92 cell line was acquired from the American Type Culture Collection and cultured in X-VIVO 10 (Lonza, 04-380Q) supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2021Quote: ... 10 pmol of the cholesterol-modified reverse primer and 1μL 20X SYBR® Green I Nucleic Acid Stain (Lonza) were mixed with the KAPA2G Fast HotStart ReadyMix in a total volume of 20 μL ...
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bioRxiv - Immunology 2021Quote: ... washed in PBS by centrifugation for 5 min at 1300 rpm at 4°C and resuspended at 4.0 × 106 cells/ml in complete medium (IMDM 2 mM glutamax I supplemented with 8% heat-inactivated FCS (Lonza), 2% heat-inactivated chicken serum ...
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bioRxiv - Immunology 2019Quote: ... two PX330-GFP vectors coding different CD80 sgRNA were electroporated into wild-type Raji cells (CD80+/CD86+/PD-L1-) using Cell Line Nucleofector Kit V (LONZA, catalog VACA-1003). Electroporated cells were recovered for two days at 37 °C/5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Vero cells were acquired from the American Type Culture Collection (ATCC #CCL-81; Manassas, VA, USA) and cultured in DMEM (Lonza, Walkersville, MD, USA) containing 2% FBS (SAFC Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... at Day 3-4 of phase I culture were electroporated using P3 Primary Cell 4D NucleofectorTM X Kit (from Lonza) with program DZ100 (Bak et al. ...
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bioRxiv - Microbiology 2023Quote: ... 190 μL was mixed with 10 μL of 20X SYBER Green I nucleic acid stain (Lonza Walkersville Inc, Walkersville, MD) in a well of a transparent 96-well plate and incubated in the dark for 15 minutes before being placed in the Guava easyCyte HT flow cytometer (488 nm ...
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bioRxiv - Pathology 2022Quote: ... using a Mitotracker® Deep RedFM (molecular probes, 50μg, reference M22426) and SYBR™ Green I (10,000x concentrate in DMSO, Lonza) staining ...
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bioRxiv - Cell Biology 2023Quote: ... hydrogels were prepared by diluting collagen I stock to a final concentration of 2.5 mg/mL with 10% v/v 10x MEM-α (BE12-684F, Lonza), 10% v/v foetal bovine serum ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative real-time PCR reactions with Taq polymerase prepared following (Pluthero, 1993) and SYBR Green I Nucleic Acid Gel Stain (Lonza) was performed in triplicates using the CFX96 real-time PCR detection system (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: The murine myoblast cell line C2C12 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and was tested for mycoplasma (MycoAlert™ Mycoplasma Detection Kit, Lonza AG, Basel, Switzerland). Cells were cultured in monolayer at 37 °C in a 5% CO2-containing humidified atmosphere in complete growth medium (GM) ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: The siRNA knockdown was performed by electroporation using the Amaxa nucleofector I in combination with the Amaxa Cell Line Nucleofector Kit V (Lonza, VCA-1003). SiRNA constructs with the siRNA cloned in the pLKO.1-Puro vector were obtained from the MISSION shRNA library (Sigma product SHGLY) ...
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bioRxiv - Immunology 2020Quote: ... 1 × 106 BLaER1 cells were transfected with 2 μg plasmid DNA using the Human B Cell Nucleofector Kit and Nucleofector I device (program U-15; both Lonza, Basel, Schweiz). On day 2 post transfection ...