Labshake search
Citations for Lonza :
101 - 150 of 224 citations for Peptide T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... Cells were electroporated using the T-028 program of a Nucleofactor™ 2b transfection device (Lonza). For the IFA analysis approximately 1/10th of the transfected cells were seeded in each well of a 24 well-plate ...
-
bioRxiv - Bioengineering 2022Quote: ... 20 μL of T cells were resuspended in P3 buffer at 5 × 107 cells/mL (Lonza) and added to the electroporation mixture ...
-
bioRxiv - Immunology 2022Quote: ... Cells were electroporated using program T-020 on the Amaxa 4D-Nucleofector (Lonza, Walkersville, MD, USA). After nucleofection ...
-
bioRxiv - Cancer Biology 2023Quote: ... T cells were washed with PBS and rested in X-VIVO 15 (#BE02-060F, Lonza, Switzerland) at a density of 1 × 106 cells per mL at 37°C ...
-
bioRxiv - Immunology 2023Quote: ... suspended in a concentration of 1.5×106 in 100 µl cell line nucleofector solution T (Lonza) and nucleofected with 5 μg of a plasmid using program X-001 in the Nucleofector IIb device (Lonza ...
-
bioRxiv - Immunology 2023Quote: ... and T cells were electroporated and transfected with TRAC RNP using a 4D-Nucleofector device (Lonza) and P3 Primary Cell Solution according to manufacturer protocol (Lonza) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Isolated naïve CD8+ T-cells were cultured in X-Vivo 15 medium (Lonza Cat. #04-418Q) supplemented with 5% human serum ...
-
bioRxiv - Synthetic Biology 2021Quote: ... This mixture was nucleofected with 2×106 stimulated human primary T cells using the 4D-Nucleofector (Lonza) with the program EO-115 ...
-
bioRxiv - Bioengineering 2021Quote: Landing pad cell lines were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with the promoterless mCherry donor containing the matching attP at a dose of either 1000 ...
-
bioRxiv - Bioengineering 2021Quote: ... 1.0×106 WT K562 were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1 μg of PiggyBac expression vector (PB200A-1 ...
-
bioRxiv - Bioengineering 2021Quote: 1.2×106 K562 cells were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 2000 ng of a pEF-1α-PuroR-P2A-mCherry donor plasmid containing an upstream Cp36 attD site (pJT371) ...
-
bioRxiv - Bioengineering 2021Quote: ... 1.2×106 K562 cells were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 2000 ng of the same Cp36 PuroR-P2A-mCherry donor ...
-
bioRxiv - Biophysics 2020Quote: ... were cultured in T-75 flasks in mammary epithelial basal medium (MEGM basal medium CC-3151; Lonza) that was supplemented with bovine pituitary extract ...
-
bioRxiv - Microbiology 2019Quote: ... brucei using the Human T-cell nucleofection® kit and an Amaxa® Nucleofector™ (Lonza AG) set to program X-001 ...
-
bioRxiv - Immunology 2020Quote: ... transferred to an electroporation cuvette and electroporated using program T-023 on an Amaxa Nucleofector (Lonza Bioscience). Cell were then left to recover for 24 to 48 hours in fresh RPMI-1640 media ...
-
bioRxiv - Immunology 2019Quote: ... T cells were centrifuged for 10 min at 90x g and resuspended in electroporation buffer P3 (Lonza) at a concentration of 750,000 cells/ 20 μL ...
-
bioRxiv - Immunology 2019Quote: ... 10×106 Jurkat T cells were electroporated using the P2 primary Cell 4D-nucleofector kit from Lonza with program EH-100 ...
-
bioRxiv - Bioengineering 2021Quote: ... 1.2×106 K562 cells were electroporated in 100 μl Amaxa solution (Lonza Nucleofector 2b, program T-016), with 300 ng of the 11.6 kb LSR plasmid ...
-
bioRxiv - Immunology 2021Quote: ... RNPs were electroporated immediately after complexing into Tregs and T cells resuspended in supplemented P3 buffer (Lonza).
-
bioRxiv - Molecular Biology 2023Quote: ... according to the manufacturer’s instructions and the T-018 program of the Amaxa Nucleofector II device (Lonza). After nucleofection ...
-
bioRxiv - Bioengineering 2023Quote: ... and CD8 T cells were purchased from the StemCell and cultured in X-VIVOTM 15 media (Lonza) supplied with 5 % human AB serum (MP Biomedicals ...
-
bioRxiv - Cancer Biology 2023Quote: ... and T cells were added at different concentrations for 48 h in X-VIVOTM 15 media (Lonza) supplemented with 5 % human AB serum (Gemini Bio ...
-
bioRxiv - Bioengineering 2023Quote: Nucleofection in T cells were performed using P3 Primary Cell 4D-Nucleofector™ X Kit S (Lonza) according to the manufacturer’s instruction with minor modifications ...
-
bioRxiv - Immunology 2023Quote: ... 1e6 T cells were resuspended in 20 µl P3 electroporation buffer with P3 supplement (Lonza. #V4XP-3032) as per manufacturer instructions and combined with 3 µl RNP ...
-
bioRxiv - Immunology 2024Quote: ... cells were washed once with PBS and resuspended in T cell nucleofection solution (Lonza, Cat: VPA-1002) containing plasmids of choice and nucleofected using the T-023 program on the Amaxa IIb nucleofector ...
-
bioRxiv - Bioengineering 2024Quote: ... Electroporation kit V and Electroporation kits for Primary T-Cell/HSPC were purchased from Lonza (Basel, Switzerland) and used on the Lonza Nucleofector 2b system ...
-
bioRxiv - Cell Biology 2022Quote: Human embryonic kidney 293 T-antigen (HEK293T) cells (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Lonza) and supplemented with 10% fetal bovine serum (Thermo Fischer Scientific) ...
-
bioRxiv - Microbiology 2021Quote: ... and 2mM L-glutamine. Spinner-adapted L929 cells (obtained from T. Dermody’s laboratory) were maintained in Joklik’s MEM (Lonza) supplemented to contain 5% FBS ...
-
bioRxiv - Microbiology 2019Quote: ... and 2 mM L-glutamine. Spinner-adapted L929 cells (obtained from T. Dermody’s laboratory) were maintained in Joklik’s MEM (Lonza) supplemented to contain 5% FBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... T cells were isolated from PBMCs as described and cultivated overnight in X-Vivo 20 (Lonza, Basel, Switzerland) supplemented with 10% human serum ...
-
bioRxiv - Immunology 2019Quote: ... Lifeact-GFP was transiently transfected into primary T cell blasts using the Amaxa 96-well shuttle system (Lonza) and the recommended protocol for activated human T cells.
-
bioRxiv - Immunology 2019Quote: ... To image T cells migrating randomly on immobilized ICAM-1 cells were resuspended in L-15 media (Lonza) supplemented with 6mM Glucose and allowed to adhere to ICAM-1 coated chambers ...
-
bioRxiv - Microbiology 2020Quote: ... Nucleofection was performed using the program X-001 for Jurkat T-cells (Amaxa™ Nucleofector™ II, Lonza). Cells were cultured in RPMI-1640 medium media supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2020Quote: ... 1 million Jurkat T-cells were resuspended in 100 μL Nucleofector solution (Cell Line Nucleofector™ Kits, Lonza) and combined with 2 μg of the linearized dual-fluorophore vector and 2 μg of the corresponding gRNA/Cas9 plasmid ...
-
bioRxiv - Cell Biology 2020Quote: Jurkat T cells (clone E6.1; ATCC TIB-152) were grown in RPMI 1640 medium w/ L-Glutamine (Lonza) supplemented with 10% Fetal Bovine Serum and 1% penicillin/streptomycin ...
-
PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... the siRNAs were transfected into human CD4+ T cells using the Amaxa Nucleofector following the manufacturer’s protocols (Lonza). The siRNAs used in this study were purchased from GenePharma and the sequences are as below:
-
bioRxiv - Molecular Biology 2023Quote: ... ∼2-4 million cells were electroporated using the T-020 method of the Nucleofector™ 2b device (Lonza). Immediately after electroporation ...
-
bioRxiv - Immunology 2023Quote: ... beads were removed on day 3 followed by electroporation using the Human T Cell Nucleofector™ Kit (Lonza) and the Amaxa Nucleofactor® 2b (Lonza ...
-
bioRxiv - Immunology 2023Quote: ... PBMCs were loaded in these wells in human T cell media (X-VIVO media, Lonza, Cat# 04-418Q) supplemented with 5% human AB serum ...
-
bioRxiv - Immunology 2023Quote: ... T cells were subsequently transfected by electroporation of RNP using setting E0115 of the Nucleofector II device (Lonza). Cells were cultured overnight at density of 3×106 cells/mL in complete RPMI supplemented with 5 ng/mL IL-7 and IL-15 to account for cell loss after electroporation (∼33%) ...
-
bioRxiv - Immunology 2023Quote: ... PBMCs were loaded in these wells in human T cell media (X-VIVO media, Lonza, Cat# 04-418Q) supplemented with 5% human AB serum ...
-
bioRxiv - Immunology 2024Quote: ... 1 - 2.5 × 106 Jurkat T cells were resuspended in EP buffer from the Nucleofector® Kit V (Lonza). Cells were combined with 1.5 μg of donor plasmid in a reaction volume of 100 μL and electroporated on the Amaxa® Nucleofector® II using pulse code X-005.
-
bioRxiv - Immunology 2023Quote: ... CTLs derived from LifeAct-GFP expressing pmel-1 mice were incubated with peptide-loaded and IFNγ-treated B16 cells cultured in phenol red-free DMEM (Lonza) supplemented with 10% FBS on stage at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... and HLA-A2+ T2 cells (can be efficiently loaded with exogenous peptides as they lack the transporter associated with antigen processing) were cultured in RPMI 1640 media (Lonza) supplemented with 10% FCS (Merck) ...
-
bioRxiv - Genetics 2020Quote: ... 5μg vector (in 5μl) transfected into 5 million CD4 T cells in 100μl 1M nucleofection solution67) using a Nucleofector 2b device (Lonza; program V024 for resting T cells and T023 for stimulated T cells) ...
-
bioRxiv - Immunology 2021Quote: ... Murine T cells were electroporated using the DN110 program of 4D nucleofector (4D-Nucleofector Core Unit: Lonza, AAF-1002B), human CAR T cells using the program E0115 ...
-
bioRxiv - Microbiology 2021Quote: ... Pre-assembled Cas9-gRNA RNPs were electroporated into cells using Lonza Human T cell Nucleofector kit (Lonza, VPA-1002) and the Nucleofector2b equipment ...
-
bioRxiv - Bioengineering 2019Quote: ... Transfection of transposon and transposase vectors was performed on day 2 with 0,5 µg transposon MC and 2 µg SB100X transposase RNA per 1×106 T cells on a 4D-Nucleofector according to the manufacturer’s instructions (Lonza). Transfected CD8+ and CD4+ T cells were expanded using the G-Rex culture system (Wilson-Wolf) ...
-
bioRxiv - Immunology 2021Quote: ... On day 7 cells were nucleofected using the Amaxa Mouse T Cell Nucleofector Kit (Lonza, Cat no. VVPA-1006) per the manufacturer’s instructions with 5x106 cells and 1.5µg plasmid DNA per cuvette ...
-
bioRxiv - Genetics 2020Quote: Vero E6 cells were cultured in T-175 flasks in Dulbecco’s modified Eagle’s medium (DMEM; Lonza, cat. 12-604F), supplemented with 8% fetal calf serum (FCS ...