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Citations for Lonza :
1 - 50 of 132 citations for PD L1 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... CD22-deleted clones modified to express PD-L1 (LZRS PD-L1) or CD200 (PB-EF-1α-intron CD200, pCMV-hyPBase, SF buffer kit V4XC-2032, Lonza 4D Nucleofector, code DN100). Transposition reactions used 1-2 μg of vector DNA and 0.5-1.0 μg of pCMV-hyPBase transposase DNA ...
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bioRxiv - Immunology 2019Quote: ... two PX330-GFP vectors coding different CD80 sgRNA were electroporated into wild-type Raji cells (CD80+/CD86+/PD-L1-) using Cell Line Nucleofector Kit V (LONZA, catalog VACA-1003). Electroporated cells were recovered for two days at 37 °C/5% CO2 ...
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bioRxiv - Immunology 2020Quote: HEK293 cells (Lonza) were cultured in DMEM (Lonza ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with pcDNA3.1 encoding full-length mouse PD-1 or its mutant using nucleofection (Lonza). Transfected cells were sorted for uniform PD-1 staining and culture in medium with 0.4 cells and reporter Jurkat cells stably expressing WT or MT chimeric PD-1 (mhPD-1 ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells were grown in DMEM medium (Lonza) with 10% fetal bovine serum (Biowest ...
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bioRxiv - Molecular Biology 2020Quote: 3T3-L1 cells were cultured in 4.5 g/L glucose DMEM (Lonza) with 10% FBS ...
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bioRxiv - Biophysics 2021Quote: ... containing AMUPol and electroporated (HEK293 pulse sequence, Lonza 4D-nucleofactor) using manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... containing AMUPol and electroporated (HEK293 pulse sequence, Lonza 4D-nucleofactor) using manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cell line was grown in DMEM medium (Lonza™ BioWhittaker™) supplemented with 10% of heat-inactivated fetal bovine serum (Biowest™ ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells (ATCC CRL-1573) were cultured in DMEM (Lonza BE12–604F) supplemented with 10% fetal calf serum (GE Healthcare ...
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bioRxiv - Systems Biology 2022Quote: ... BJ5ta cells and HEK293 cells were transfected using the 4D nucleofector (Lonza) and Neon transfection system (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: HeLa and HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Lonza) containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were grown in DMEM containing high glucose and sodium pyruvate (Lonza) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Genomics 2023Quote: ... 0.5 µg of these constructs were nucleofected into HEK293 cells using 4D Nucleofector (Lonza) and luciferase activity was measured 24 h post transfection using Spectramax plate reader (Molecular Devices) ...
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bioRxiv - Neuroscience 2022Quote: HEK293 Sham and HEK293_GLT8D1-WT/R92C stable cell lines were cultured in 10mL DMEM (Lonza) supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2022Quote: ... the plasmids mEmerald-JUP-C-14 and mEmerald-Beta-Catenin-20 were transfected in 3T3-L1 pre-adipocytes by Nucleofection (Lonza). mEmerald-JUP-C-14 (Addgene plasmid # 54132 ...
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bioRxiv - Neuroscience 2023Quote: The modified pC13 dCas9-BFP-ZIM3 construct with CLYBL homology arms was co-nucleofected into 125Q iPSC with two additional constructs carrying TALENs targeting the CLYBL locus (pZT-C13-R1 and pZT-C13-L1, addgene #62196, #62197 (Cerbini et al., 2015)) in P3 solution using program CA137 in a 4D-Nucleofector (Lonza). Cells were seeded at clonal density and selection in G418 was initiated 48 hours later ...
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bioRxiv - Microbiology 2021Quote: HEK293 cells (obtained from M. Marketon’s laboratory) were maintained in Dulbecco’s modified essential medium (DMEM) (Lonza) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Physiology 2024Quote: The gRNA plasmid (1µg) and donor template plasmid (3µg) were introduced into 3T3-L1 cells (1X 10 6 cell) by electroporation (LONZA, Basel, Switzerland). After 48h of electroporation ...
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bioRxiv - Cell Biology 2021Quote: HEK293 (human embryonic kidney 293) cells were grown in Dulbeccos Modified Eagles Medium (DMEM, Lonza, Basel, Switzerland) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... 1·106 of HEK293 cells were resuspended in 100 μL of pre-warmed Nucleofector solution V (Lonza). The plasmid containing c17orf80 gRNA and Cas9 (4 μg ...
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bioRxiv - Immunology 2022Quote: ... Co-cultures were done in IMDM supplemented with 5% HI-HS (Lonza) and gentamycin (86 μg/mL ...
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bioRxiv - Bioengineering 2019Quote: Cell culture: Glut1 experiments were done on HEK293 (ATCC #CRL-1573) cells transfected using the Amaxa system (Lonza) and 1 µg DNA ...
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bioRxiv - Microbiology 2019Quote: ... and 25 ng/ml of amphotericin B. HEK293 cells (obtained from M. Marketon’s laboratory) were maintained in Dulbecco’s modified essential medium (DMEM) (Lonza) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: HeLa cells and HEK293 cells obtained from Melanie Marketon’s lab at Indiana University were maintained in Dulbecco’s MEM (Lonza) supplemented to 5% FBS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: HEK293 cells were passaged in poly-D-Lysine treated plates and incubated overnight in OPTI-MEM medium (Lonza) at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: Human embryonic kidney 293 (HEK293) cells (ATCC; CRL-1573) were grown in high-glucose DMEM (Lonza; BE12-604F) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2023Quote: Plasma cholesterol was depleted by treating HEK293 cells with 5 mM MβCD for 30 min in Pro293A-CDM (Lonza); this short period of MβCD treatment was deemed sufficient to removed 50% of endogenous cholesterol from cells (34) ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were washed twice with PBS and 2 × 106 cells were resuspended in 100 μl of Solution V (Lonza) and then mixed with 5 μg purified sgRNA expression vector or the empty vector as a negative control before nucleofection using program Q-001 of a Nucleofector™ 2b (Lonza) ...
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bioRxiv - Biophysics 2019Quote: The HEK293 adherent culture cells were transfected with pcDNA3.4-PCV2d plasmid using Amaxa Nucleofector II instrument by Lonza (program A-23). The transfected cell were plated on 6-well plate ...
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bioRxiv - Neuroscience 2019Quote: ... Co-transfection of 500 ng sgRNA plasmid and 1 µl of 10 µM ssODN template into 2×105 HEK293 cells was performed using nucleofection on the 4D-Nucleofector system (LONZA, Basel, Switzerland), as described previously38 ...
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bioRxiv - Cell Biology 2022Quote: ... Transient transfections of these dominant-negative Rab11 a and siRab11 a into mouse MΦs (RAW 264.7 cell line) were performed with Amaxa mouse macrophage nucleofector kit (VPA-1009, Lonza) and DharmaFECT 4 Transfection Reagent (T-2004-01 ...
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bioRxiv - Neuroscience 2020Quote: ... and the Mouse Neuron Nucleofector Kit (Lonza), following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... using the mouse ES Cell nucleofector kit (Lonza) and Amaxa Nucleofector II device (Lonza ...
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bioRxiv - Developmental Biology 2021Quote: ... using the mouse ES cell nucleofection kit (Lonza) using protocol “A24” ...
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bioRxiv - Bioengineering 2023Quote: Mouse C57 Cortex Neurons (Lonza, M-CX-300) were cultured in Primary Neuron Basal Medium (PNBM ...
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bioRxiv - Neuroscience 2023Quote: ... and mouse neuron nucleofectorTM kit (VPG-1001, Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... X-Unit (Lonza, program: unstimulated mouse T cells). Cells were washed immediately after nucleofection to remove nucleofection reagents and improve survival.
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bioRxiv - Immunology 2024Quote: ... with an Amaxa Mouse Dendritic Cell Nucleofection Kit (Lonza). The abovementioned pIRES2-AcGFP1-series plasmids were used for transient expression ...
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bioRxiv - Neuroscience 2021Quote: ... using an Amaxa Mouse Neuron Nucleofector kit (Lonza, VPG-1001) as described previously (Wuttke et al. ...
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bioRxiv - Systems Biology 2023Quote: ... and Mouse Embryonic Stem Cell NucleofectorTM Kit (#VPH-1001, Lonza). Three biological replicates were performed per library on different days ...
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bioRxiv - Immunology 2023Quote: ... using a mouse T cell nucleofection kit (Lonza, VPA-1006), and rested overnight in R10 medium ...
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bioRxiv - Molecular Biology 2023Quote: ... With the mouse ES Cell Nucleofector Kit (Lonza VPH-1001), PiggyBAC and pBASE plasmid are co-transfected into mESCs ...
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bioRxiv - Cancer Biology 2021Quote: ... expressing Cas9 and sgRNA targeting mouse p53 and pmaxGFP plasmid (Lonza). Similarly ...
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bioRxiv - Genetics 2021Quote: ... and Mouse Embryonic Stem Cell Nucleofector™ Kit (#VPH-1001, Lonza). Klf2 and Nanog loci Upstream assay libraries were mixed and transfected together ...
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bioRxiv - Neuroscience 2020Quote: ... France) by nucleofection using the AMAXA Mouse Neuron Nucleofector Kit (Lonza). Each transfection was performed with 1.5 × 106 hippocampal neurons and plated on three video microscopy dishes (ibidi).
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bioRxiv - Developmental Biology 2019Quote: ... Nucleofection was performed with the Nucleofector Kit for mouse ESC (Lonza), using 2.5 μg of DNA and the Amaxa A-013 program ...
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bioRxiv - Cell Biology 2024Quote: ... The Amaxa Mouse Macrophage Nucleofector Kit was from Lonza (Cologne, Germany). Gene-specific TaqMan primer-probe mixes used for quantitative real-time polymerase chain reaction (PCR ...
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bioRxiv - Cell Biology 2024Quote: ... Cell clumps were electroporated using Mouse/Rat Hepatocyte NucleofectorTM Kit (Lonza) and Amaxa Nucleofector® 1 device in the presence or absence of flagellin (1μg ...
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bioRxiv - Immunology 2024Quote: ... using the Amaxa Mouse Dendritic Cell Nucleofector Kit (#VPA-1011, Lonza) as previously described (12 ...