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Citations for Lonza :
251 - 300 of 377 citations for Mouse ZNF583 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... This plasmid and plasmid pSPCas9(BB)-2A-puro(PX459)-hMyo10 above were transfected together into HeLa cells using an Amaxa nucleofection apparatus (Lonza). Five days later the cells were subjected to single-cell sorting into 96-well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... containing a combination of the px458 plasmid targeting each gene and the ssODN and then electroporated using B-016 program of Nucleofector 2b (Lonza). The electroporated GSCs were cultured for 48 h followed by Single cell sorting of GFP-positive cells (SH800 ...
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bioRxiv - Physiology 2023Quote: ... 2 μl of KCNQ2/3 cDNA plasmid (0.9 g/l) and 1 μl of pmax green fluorescent protein vector (0.5 g/l) (Lonza, Cologne, Germany) were diluted in 125 μl of Opti-MEM medium (Life Technologies) ...
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bioRxiv - Developmental Biology 2023Quote: ... The RFP hESC line was generated following introduction of a pCAG-H2B-RFP plasmid (Price et al., 2021) into H9 hESCs using a 4D-Nucleofector (Lonza). After puromycin selection (1µg/ml) ...
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bioRxiv - Cancer Biology 2022Quote: ... An iCasp9-CD20/CD19-CAR transposon minicircle carrying CD20 scFv[17] and CD19 scFv[18] and a qPBase plasmid were delivered into activated T cells using 4D-Nucleofector device (Lonza) and Quantum Nufect Kit (GenomeFrontier Therapeutics ...
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bioRxiv - Genomics 2023Quote: ... and 20 μg of pDC2 plasmid with the required cloned guide DNA and electroporated using an Amaxa 4D-Nucleofactor (Lonza), using program FP158 as previously described45 ...
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bioRxiv - Cell Biology 2023Quote: ... Five million BJAB or OCI-LY1 cells were transfected with 2 μg plasmid DNA using the SF Cell Line 4D-Nucleofector X Kit L (Lonza) and the AMAXA Nucleofector biosystem (BJAB ...
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bioRxiv - Microbiology 2023Quote: ... Stable cell lines were generated by nucleofecting 2 µg of pBud-PCID2-Flag plasmid or empty pBud as control using Amaxa Nucleofector (Lonza) and Nucleofector Kit R (Lonza ...
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bioRxiv - Cell Biology 2023Quote: WT cells were transfected with the sgRNA-containing plasmids by electroporation with a Nucleofector device (Lonza; in Mirus transfection solution). Cells were plated in culture dishes and maintained for three days ...
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bioRxiv - Neuroscience 2023Quote: ... reprogramming was initiated by nucleofection of 1×105 fibroblast with 1 µg of each episomal plasmid (pCXLE-hUL, pCXLE-hSK and pCXLE-hOCT4) using the Nucleofector 2b (Lonza). Initially ...
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bioRxiv - Cell Biology 2024Quote: ... Two million E14 ESCs were nucleofected with paired Kdm3b targeting and donor plasmids (5 µg each) using the Amaxa 4D-Nucleofector protocol (Lonza) and selected with blasticidin S hydrochloride (Research Products International ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were nucleofected with plasmids of interest using the Lonza 2b Nucleofector and Rat cardiomyocyte Nucleofector Kit (Lonza, VAPE-1002) according to the manufacturer’s instruction.
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bioRxiv - Neuroscience 2023Quote: ... Neurons were nucleofected with plasmids of interest using the Lonza 2b Nucleofector and Rat Neuron Nucleofector Kit (Lonza, VPG-1003) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... They were then transfected by nucleofection with 3 μg of plasmid DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza) using the V-001 program (Amaxa Biosystems) ...
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bioRxiv - Biochemistry 2022Quote: ... CD-1 mouse embryonic fibroblasts (MEFs) were obtained from Lonza (Cat#M-FB-481) and inactivated with 10 μg ml−1 mitomycin C (Sigma-Aldrich ...
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bioRxiv - Genomics 2022Quote: ... Nucleofection solutions and cuvette were from Mouse ES Cell Nucleofector kit (Lonza, VPH-1001). Nucleofector (Lonza 2b ...
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bioRxiv - Neuroscience 2021Quote: ... An Amaxa Nucleofector 2b Device and a Mouse Embryonic Fibroblast Nucleofector Kit 1 (Lonza) were used to transfect MEFs with βCTF-3xFLAG plasmid using program N-024 of the Nucleofector (Supplementary Figure 3).
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bioRxiv - Genetics 2022Quote: CRISPR plasmid constructs (2 μg) were electroporated into 2 × 106 Raji cells using the Amaxa Cell Line Nucleofector Kit V (Lonza Bioscience) (Program M-013) ...
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bioRxiv - Molecular Biology 2022Quote: ... Percoll-purified (82) late-schizont-stage parasites were transfected with 50 µg of plasmid DNA using Amaxa Nucleofector 2b (Lonza, Switzerland) as previously described(83) ...
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bioRxiv - Neuroscience 2020Quote: Primary rat hippocampal neurons were prepared as described previously39) Cultured neurons were transfected with pCAG-FR-GECO1a and pCAG-FR-GECO1c plasmids using electroporation (Lonza Nucleofector) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Transient transfection of pp150 in HFF was performed by transfecting pp150 expressing plasmid (kindly provided by Edward Mocarski) using Amaxa Nucleofection kit V (Lonza inc.). The pp71-expressing plasmid (pCGN-pp71 ...
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bioRxiv - Biophysics 2019Quote: The HEK293 adherent culture cells were transfected with pcDNA3.4-PCV2d plasmid using Amaxa Nucleofector II instrument by Lonza (program A-23). The transfected cell were plated on 6-well plate ...
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bioRxiv - Neuroscience 2019Quote: ... or a non-silencing plasmid immediately before plating (Amaxa™ P3 Primary Cell 4D-Nucleofector X Kit L; Lonza, CU133 program). The generation and knockdown efficiencies of shRNA plasmids were described previously (Guner et al. ...
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bioRxiv - Genomics 2021Quote: ... K562 cells were grown to a density of 1×106 cells and electroporated with 5μg of plasmid DNA (Amaxa® Cell Line Nucleofector® Kit V, Lonza). Cells from each cell line were cultured for an additional 48 hours until total RNA isolation (Maxwell RSC simplyRNA kit ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RBD-Fc stable clone was obtained by electroporation with 2×106 cells and 5 μg endotoxin-free plasmids using Amaxa kit V and program U24 with Amaxa Nucleofector 2B (Lonza, Switzerland). Electroporated cells were subsequently plated in 96-wells at 500 cells/well in Plating Medium containing 80% EX CELL® CHO Cloning Medium (Cat.no C6366 ...
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bioRxiv - Developmental Biology 2022Quote: ... 400,000 H9 cells for each condition were electroporated with 1 μg plasmid via a human stem cell nucleofector™ kit (VPH-5012) by Lonza AMAXA Nucleofector 2B.
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bioRxiv - Genomics 2022Quote: ... We used 1.0 × 106 HCT116 or U2OS cells to transfect the base editor plasmids (2600 ng) using SE solution and 4D-nucleofector (Lonza, Switzerland) as per the manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: ... 1 million cells were washed with PBS and electroporated with 5-10 μg of appropriate plasmid using Amaxa cell nucleofector kit T (Lonza laboratories). Cells were allowed to recover for 48 h ...
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bioRxiv - Physiology 2022Quote: ... 5 million cells were washed with phosphate buffered saline (PBS) and electroporated with 4-6 μg of appropriate plasmid construct using an Amaxa cell nucleofector (Lonza laboratories) and nucleofection reagent (362.88 mM ATP-disodium salt ...
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bioRxiv - Neuroscience 2022Quote: Bulk electroporations were performed with endotoxin-free plasmid preparations (>0.9 μg/μl) using the Y-unit of the 4D-Nucleofector® (Lonza Bioscience), both HcKCR1 and WiChR were subcloned into a pAAV-backbone and expressed as fusion proteins tagged with a fluorophore under a human syn1 promoter ...
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bioRxiv - Molecular Biology 2022Quote: ... Percoll-purified (70) parasites at late schizont stage were transfected with 50 μg plasmid DNA using Amaxa Nucleofector 2b (Lonza, Switzerland) as previously described (78) ...
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bioRxiv - Cell Biology 2023Quote: ... Percoll-purified [131] parasites at late schizont stage were transfected with 50 µg plasmid DNA using Amaxa Nucleofector 2b (Lonza, Switzerland) as previously described [135] ...
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bioRxiv - Physiology 2024Quote: The gRNA plasmid (1µg) and donor template plasmid (3µg) were introduced into 3T3-L1 cells (1X 10 6 cell) by electroporation (LONZA, Basel, Switzerland). After 48h of electroporation ...
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bioRxiv - Cell Biology 2023Quote: ... brucei 13-90 cells were electroporated with 10 µg of linearised plasmid DNA using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (program X-001) (Lonza, Switzerland).
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bioRxiv - Immunology 2024Quote: ... JurkatE6-1 cells and CD3+ T cells isolated from tumors or PBMCs were transfected with the OSA plasmid using the preset program DS104 of the 4D-Nucleofector™ system (Lonza). Electroporation was performed following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: HeLa and Mouse Embryonic Fibroblast (MEF) cells were grown in Dulbecco’s modified Eagle’s medium (Lonza) supplemented with 10% heat-inactivated fetal bovine serum (Atlanta Biologicals) ...
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bioRxiv - Neuroscience 2024Quote: ... Transfections of primary cells were all performed with the Amaxa Mouse Neuron Nucleofector Kit (Lonza), using the O-005 nucleofection program ...
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bioRxiv - Biophysics 2021Quote: ... The WT and mutant genes were then amplified from these expression plasmids using the PCR primers 5’-GCGCTAGCAGCACCCATATGCCTGAG-3’and 5’-ACGAAGCTTTCAGTGGTGGTGGTGGT-3’and subcloned into the pMaxCloning vector (Lonza, VDC-1040) via NheI and BamHI sites to generate the pMax_WT_NEIL1 and pMax_Mutant_NEIL1 expression plasmids that were utilized throughout this analysis.
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bioRxiv - Cell Biology 2020Quote: 1 million Jurkat cells were electroporated with the 2.5 μg of sgRNA plasmid and GFP control plasmid at a 10:1 ratio using an Amaxa Cell Line Nucleofector Kit V and an Amaxa™ Nucleofector™ II (Lonza). HeLa ...
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bioRxiv - Microbiology 2021Quote: ... Excysted sporozoites were resuspended in transfection buffer supplemented with a total of 100 µg DNA (comprised of 50 µg of Cas9/gRNA plasmid and 50 µg of repair template generated by PCR) and nucleofected using an Amaxa 4D nucleofector (Lonza, Basel, Switzerland). Transfected parasites were resuspended in PBS and administered to Ifng-/- mice ...
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bioRxiv - Developmental Biology 2020Quote: ... about 8 × 105 iPSCs were transfected with 5 μg of plasmids with Lonza Human Stem Cell Nucleofector Kit 1 (Lonza, VPH-5012) on a Nucleofector 2b device (Lonza ...
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bioRxiv - Cell Biology 2020Quote: ... These plasmids were transfected into NHDFs and 7SPs by electroporation using the Amaxa nucleofector kit and nucleofector 2b (Lonza, Walkersvill, MD, USA). Two days later ...
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bioRxiv - Cell Biology 2021Quote: ... 500μg of gRNA and 500μg of donor plasmids were electroporated in 1×106 mESC using P3 Primary Cell 96-well Nucleofector™ Kit (Lonza, PBP3-22500) according to manufacturer’s instruction ...
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bioRxiv - Genomics 2022Quote: ... 8 million cells were transfected with 20ug of plasmid DNA in one 100- uL cuvette using the Amaxa® Cell Line Nucleofector® Kit V (Lonza), program X-001 ...
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bioRxiv - Genomics 2022Quote: ... 5 million cells were transfected with 10ug of plasmid DNA in one 100- uL cuvette using the Amaxa® Cell Line Nucleofector® Kit T (Lonza), program X-001 ...
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bioRxiv - Microbiology 2022Quote: ... 1.2E6 BHK21 cells were nucleofected with 2 µg of Spike plasmid using an Amaxa Nucleofector II with cell line kit L (Lonza #VCA-1005) and program A-031 ...
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bioRxiv - Microbiology 2022Quote: ... schizonts purified from an overnight culture of PbDiCre parasites were transfected with 5–10 µg of linearised plasmid by electroporation using the AMAXA Nucleofector device (Lonza, program U033), as previously described (Janse et al. ...
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bioRxiv - Biophysics 2022Quote: ... 106 U2OS cells were transfected with 1 μg of px330 plasmid and 1 μg of the HDR donor plasmid using the Nucleofector 2b device and cell line nucleofector kit V (Lonza, VCA-1003) per manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... and either the HSP-Luc or the NF-κB-HSP-Luc plasmids by nucleofection using Amaxa Nucleofector (Amaxa AG-Lonza, Cologne, Germany) as previously described 59 ...
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bioRxiv - Neuroscience 2021Quote: ... Single-cell suspension of hiPSCs was nucleofected with 5 μg of the generated SpCas9-sgRNA plasmid using the P3 Primary Cell 4D-Nucleofector Kit (Lonza, #V4XP-3024) in combination with the 4D Nucleofector Unit X (Lonza ...