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Citations for Lonza :
51 - 100 of 2347 citations for Mouse Calcitonin Gene Related Peptide Type 1 Receptor CALCRL ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: The murine myoblast cell line C2C12 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and was tested for mycoplasma (MycoAlert™ Mycoplasma Detection Kit, Lonza AG, Basel, Switzerland). Cells were cultured in monolayer at 37 °C in a 5% CO2-containing humidified atmosphere in complete growth medium (GM) ...
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bioRxiv - Bioengineering 2019Quote: ... The plate containing the amplified genes or qPCR products was kept in ice and observed in a 2% agarose gel (NuSieve® 3:1 Agarose (Lonza)) to check and reinforce the identity of the amplicons ...
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bioRxiv - Physiology 2023Quote: Microvascular endothelial cells from healthy and Type II diabetic humans (Lonza) were cultured to passage 2-3 in EBM-2 media supplemented with microvascular endothelial cell growth medium SingleQuots supplements (EGM-2 MV ...
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bioRxiv - Genomics 2022Quote: ... Nucleofection solutions and cuvette were from Mouse ES Cell Nucleofector kit (Lonza, VPH-1001). Nucleofector (Lonza 2b ...
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bioRxiv - Cancer Biology 2022Quote: ... 3.6 Supplement 1 (Lonza kit, #V4XP-3032), and 1ug of plasmid DNA or 200pmol of chemically stabilized synthetic RNA (Synthego Corp.) ...
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bioRxiv - Immunology 2024Quote: 1-1.5 x106 triple reporter TCR-/- Jurkat cells were mixed with 2μg of pLVM containing gene of interest and electroporated using pulse code CL-120 according to manufacturer’s protocol (Lonza #V4XC-1024, SE Cell line kit). After 48-72hrs ...
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bioRxiv - Neuroscience 2024Quote: ... Transfections of primary cells were all performed with the Amaxa Mouse Neuron Nucleofector Kit (Lonza), using the O-005 nucleofection program ...
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bioRxiv - Cell Biology 2021Quote: ... the media was changed to α-MEM containing macrophage colony-stimulating factor (M-CSF; 10 ng/ml) and and 66ng/ml receptor activator of nuclear factor kappa-B ligand (RANKL, Lonza Biosciences). OC activity was measured by releasing the europium conjugated human Type I collagen-coated on the bottom of the OsteoLyse™ Assay Kit (Lonza Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: Xist knockdown was performed following manufacturer’s instructions for the Mouse Neural Stem Cell Nucleofector Kit (Lonza). 3-5×106 NSCs were collected and resuspended in 70 μL Nucleofector solution ...
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bioRxiv - Genetics 2021Quote: ... Transfection was carried out by electroporation using Mouse Embryonic Stem Cell Nucleofector™ Kit from Lonza. After 2 days of transfection GFP expressing cells were FACS sorted and sparsely seeded for clonal expansion on 15cm plate ...
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bioRxiv - Genomics 2021Quote: ... using a Nucleofector™ 2b device and the Mouse ES Cell Nucleofector Kit (Lonza, VAPH-1001) according to the manufacturer’s instructions and plated into two 10 cm dishes ...
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bioRxiv - Cell Biology 2024Quote: ... T47D cells were transiently transfected with the avian tumor virus receptor A (TVA) by using electroporation (preset “FF150” program, 4D-Nucleofector transfection system; Lonza, Cologne, Germany). The cell suspension was cultured with full medium at 37 °C and 5% CO2 for 24 hours ...
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bioRxiv - Biochemistry 2022Quote: ... CD-1 mouse embryonic fibroblasts (MEFs) were obtained from Lonza (Cat#M-FB-481) and inactivated with 10 μg ml−1 mitomycin C (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: The dissociated sensory neuron pellet was resuspended with 98 µL electroporation buffer (Mouse neuron nucleofector kit, Lonza) mixed with RNA oligos (0.2 nmol per transfection ...
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bioRxiv - Biochemistry 2022Quote: ... electroporation was carried out following the instructions of the Mouse Embryonic Stem Cell Nucleofector Kit from Lonza. After 2 days recovery ...
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bioRxiv - Immunology 2020Quote: ... American Type Culture Collection (ATCC)] were grown in Eagle’s minimal essential medium (EMEM; Lonza) supplemented with 8% fetal calf serum (FCS ...
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bioRxiv - Immunology 2021Quote: ... American Type Culture Collection (ATCC)] were grown in Eagle’s minimal essential medium (EMEM; Lonza) supplemented with 8% fetal calf serum (FCS ...
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bioRxiv - Bioengineering 2023Quote: ... (Click Chemistry Tools):Peptides for an hour at room temperature in Dulbecco’s Phosphate Buffered Saline (DPBS) (BioWhittaker, Lonza). The conjugates were further incubated with 1.1:1 Cyanine 5 trans-cyclooctene (AAT Bioquest):Met-NHS for a minute and run through NuPAGE 4-12% Bis-Tris premade polyacrylamide gel (Invitrogen by Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... 5 × 106 trypsinized cells were resuspended in 93.5 µl solution (mouse ES cell nucleofector kit, VPH-1001, Lonza). The solution includes 2 µg of each CRISPR/Cas9 vector (4 µg total ...
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bioRxiv - Pathology 2020Quote: ... Plasmid delivery through electroporation of passage three neurospheres was performed using Mouse neural stem cell nucleofector kit (Lonza).
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bioRxiv - Immunology 2023Quote: Electroporation was performed to introduce siRNA into BMDCs using a mouse dendritic cell nucleofector kit (Lonza, Basel, Switzerland) and a Nucleofector 2b (Lonza) ...
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bioRxiv - Genomics 2021Quote: ... were plated on type I collagen-coated 24-well plates in plating medium (MP100, Lonza) and maintained in hepatocyte growth medium (HCM ...
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bioRxiv - Developmental Biology 2021Quote: ... pPBCAG-hph and a PiggyBac Transposase vector using the AMAXA Mouse Embryonic Stem Cell Nucleofector Kit (LONZA, VPH-1001). Transfected cells were selected with 200 μg/ml hygromycin B Gold (Ibian tech. ...
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bioRxiv - Developmental Biology 2021Quote: ... 5×106 EL16.7 TST ESCs were nucleofected with the AMAXA Mouse Embryonic Stem Cell Nucleofector Kit (LONZA, VPH-1001) using program A-30 with 1.6 µg each of GFP and tdTomato circularised targeting vectors and 5 µg single gRNA vector PX459 (5’-TATACCTAATCATTATGCCG-3’ ...
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bioRxiv - Cancer Biology 2021Quote: ... The siRNA transfections for primary BMDMs and pancreatic fibroblasts were performed using the Mouse Macrophage Nucleofector™ Kit (Lonza) and Nucleofector™ 2b Device (Lonza ...
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bioRxiv - Immunology 2021Quote: ... On day 7 cells were nucleofected using the Amaxa Mouse T Cell Nucleofector Kit (Lonza, Cat no. VVPA-1006) per the manufacturer’s instructions with 5x106 cells and 1.5µg plasmid DNA per cuvette ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs were immortalized with pBabe-SV40Tag by electroporation using the Amaxa Mouse/Rat Hepatocyte Nucleofector Kit (Lonza, VPL-1004). Immortalized MEF lines were genotyped ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with pcDNA3.1 encoding full-length mouse PD-1 or its mutant using nucleofection (Lonza). Transfected cells were sorted for uniform PD-1 staining and culture in medium with 0.4 cells and reporter Jurkat cells stably expressing WT or MT chimeric PD-1 (mhPD-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 500,000 cells were transfected using the using the mouse neural stem cell nucleofection kit (Lonza, VPG-1004) with 0.66 μg of the construct and 0.33 μg of transposase using the Lonza Amaxa Nucleofector I with protocol A-33 ...
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bioRxiv - Neuroscience 2021Quote: ... the cell suspension was transfected with a Homer 1c::TdimerDsRed plasmid (Bats et al., 2007) by electroporation using the Amaxa Nucleofector 2b device and Amaxa mouse neuron nucleofector kit (Lonza). Neurons were then plated on poly-L-lysine-coated glass coverslips and grown at 37°C in a humidified 5% CO2-containing atmosphere in BME supplemented with KCl (25 mM final concentration) ...
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bioRxiv - Immunology 2022Quote: ... Knockdown of Vps4a/b was performed using Amaxa Nucleofector II and the Mouse T-Cell Nucleofector Kit (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Nucleofection was performed on an Amaxa nucleofector IIb using protocol O-003 with the mouse neuron kit (Lonza, VPG-1001). Cells were allowed to recover for 1 hour at 37 °C and followed by NCAM1 MACS and culture as described above.
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bioRxiv - Cell Biology 2021Quote: ... was generated by the introduction of a plasmid carrying the EGFP gene under the control of the CAG promoter to EStTA5-4 cells using the Amaxa Mouse ES cells Nucleofector Kit (VPH-1001, Lonza).
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bioRxiv - Cell Biology 2020Quote: ... 10 µg of plasmids were mixed with 5 millions ESC in buffer “Mouse ES cell nucleofector kit (Lonza, VPH-1001) per electroporation ...
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bioRxiv - Genetics 2021Quote: ... first excised floxed Neomycin resistance cassette from Oct4 (CiA:Oct4) mESCs 12 by transfecting Cre-mCherry plasmid using Mouse Embryonic Stem Cell Nucleofector™ Kit from Lonza. After 24 hours after transfection ...
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bioRxiv - Genetics 2021Quote: ... Cas9-sgRNA-Thy1.1 plasmid transfection was carried out by electroporation using Mouse Embryonic Stem Cell Nucleofector™ Kit from Lonza. After 36 hours of transfection Thy1.1 expressing cells were FACS sorted and sparsely seeded for clonal expansion on 15cm plate ...
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bioRxiv - Neuroscience 2023Quote: Transfection of dissociated neurons was performed using the AMAXA Nucleofector system (program O.005) with the mouse neuron Nucleofector kit (Lonza) following the manufacturer’s specifications ...
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bioRxiv - Immunology 2023Quote: Hepatocytes (7×105 cells) were transfected with various plasmids (at 6 μM or indicated concentrations) using the Mouse/ Rat hepatocyte Nucleofector kit (Lonza) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were nucleofected with 4 µg of the sgRNA-containing plasmid individually following the Amaxa Mouse ES cell Nucleofector kit recommendations (VPH-1001, Lonza). Later ...
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bioRxiv - Cell Biology 2023Quote: ... JAWS transfection was achieved using 20 μg DNA and 2 x 106 million cells per 100 μl nucleofection solution from the Amaxa Nucleofector Mouse Dendritic Cell kit (Lonza), and the Y-001 program ...
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bioRxiv - Systems Biology 2024Quote: The reporter cell line for the PTGR screen was created by nucleofection of haploid AN3-12 mESCs with 500ng of the reporter construct and 10 µg of a Tol2 transposase encoding plasmid using the Mouse ES Cell Nucleofector Kit (Lonza) according to the manufacturer’s protocol using an Amaxa Nucleofector (Lonza) ...
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bioRxiv - Cancer Biology 2022Quote: ... UWB1 and UWB1+BRCA1 cells were maintained in RPMI1640 (ATCC) and MEGM bullet kit (1:1; Lonza) with 3% FBS ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Human Stem Cell Nucleofector Kit 1 (Cat. No. VPH-5012) and mESC Nucleofector Kit (Lonza, Cat. No. VVPH-1001) were used for delivering DNA into human and mouse ESCs ...
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bioRxiv - Molecular Biology 2023Quote: One million Aire wild-type mTEC 3.10 cells were suspended in 100 μL of nucleofector solution (Lonza) containing 20 pmol of spCas9-GFP ...
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bioRxiv - Developmental Biology 2020Quote: ... and nucleofected with BioID constructs using an Amaxa Nucleofector II and the Mouse Neural Stem Cell Nucleofector Kit (Lonza; VPG-1004) according the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... 4 μg of the donor ssDNA and 3 μg of a puromycin resistance plasmid (pCAG-puroR) using the Mouse ES Cell Nucleofector™ Kit (Lonza) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... E14 cells (5 × 106) were transfected with 10 μg of the appropriate plasmid with the Mouse ES Cell Nucleofector™ Kit (Lonza) and allowed to grow for 48h after transfection ...
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bioRxiv - Genetics 2021Quote: ... complex and mix of 1:1 ratio WT/mutant donor single-stranded oligodeoxynucleotides carrying PAM mutation by electroporation using Mouse Embryonic Stem Cell Nucleofector™ Kit from Lonza. sgRNA and donor single-stranded oligodeoxynucleotide sequences can be found in supplementary table 5 ...
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bioRxiv - Immunology 2020Quote: ... and pmaxGFP (0.5 ug; Amaxa Noclecfector Kit V for THP-1, Lonza). All transfection experiments were performed with Amaxa Cell Line Nucleofector Kit V for monocytic cells(Lonza ...
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bioRxiv - Biochemistry 2023Quote: ... 400 000 MNT-1 cells were transfected using nucleofection (NHEM kit, Lonza) on Amaxa device 2 (program T20 ...