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Citations for Lonza :
51 - 100 of 127 citations for Mouse Anti Zika Virus NS1 Antibody B4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: Mouse embryonic fibroblasts (MEFs) cells were grown in Dulbecco’s modified Eagle’s medium DMEM (12-614F, Lonza), supplemented with 5% fetal bovine serum and non-essential amino acids at 37°C and 5% CO2.
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bioRxiv - Molecular Biology 2021Quote: Xist knockdown was performed following manufacturer’s instructions for the Mouse Neural Stem Cell Nucleofector Kit (Lonza). 3-5×106 NSCs were collected and resuspended in 70 μL Nucleofector solution ...
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bioRxiv - Immunology 2019Quote: ... cells rested overnight in complete medium (human cells) or X-Vivo 15™ (mouse cells) (Lonza) were activated with IL-2 (100U/ml ...
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bioRxiv - Genetics 2021Quote: ... Transfection was carried out by electroporation using Mouse Embryonic Stem Cell Nucleofector™ Kit from Lonza. After 2 days of transfection GFP expressing cells were FACS sorted and sparsely seeded for clonal expansion on 15cm plate ...
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bioRxiv - Genomics 2021Quote: ... using a Nucleofector™ 2b device and the Mouse ES Cell Nucleofector Kit (Lonza, VAPH-1001) according to the manufacturer’s instructions and plated into two 10 cm dishes ...
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bioRxiv - Neuroscience 2020Quote: The dissociated sensory neuron pellet was resuspended with 98 µL electroporation buffer (Mouse neuron nucleofector kit, Lonza) mixed with RNA oligos (0.2 nmol per transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... Smarca4f/f mouse lines) or 20,000 AALE cells were plated on a 0.35% low melting agarose (Lonza) placed over a 0.7% agarose layer in a 6-well plate ...
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bioRxiv - Developmental Biology 2022Quote: ... Nucleofection experiments were performed following the manufactures’ protocol for mouse embryonic fibroblasts (Amaxa™ Nucleofector™, Lonza). To induce iFoxn1 expression ...
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bioRxiv - Neuroscience 2020Quote: ... mouse neurons were transfected before plating with 5 μg of MAP2-GFP plasmid using a Nucleofector (Lonza) according to the manufacturer’s specifications ...
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bioRxiv - Biochemistry 2022Quote: ... electroporation was carried out following the instructions of the Mouse Embryonic Stem Cell Nucleofector Kit from Lonza. After 2 days recovery ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with pcDNA3.1 encoding full-length mouse PD-1 or its mutant using nucleofection (Lonza). Transfected cells were sorted for uniform PD-1 staining and culture in medium with 0.4 cells and reporter Jurkat cells stably expressing WT or MT chimeric PD-1 (mhPD-1 ...
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bioRxiv - Molecular Biology 2021Quote: Human and mouse fibroblasts were cultured at 37°C and 5% CO2 in Dulbecco’s modified Eagle’s medium (Lonza) with high glucose supplemented with 10% foetal bovine serum ...
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bioRxiv - Neuroscience 2022Quote: ... 5 × 106 trypsinized cells were resuspended in 93.5 µl solution (mouse ES cell nucleofector kit, VPH-1001, Lonza). The solution includes 2 µg of each CRISPR/Cas9 vector (4 µg total ...
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bioRxiv - Developmental Biology 2022Quote: ... and pre-seeded with low density (8,000 cells/cm2) irradiated DR4 mouse embryonic fibroblast (MEF) feeder cells (Lonza) (Matrigel-MEF) ...
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bioRxiv - Pathology 2020Quote: ... Plasmid delivery through electroporation of passage three neurospheres was performed using Mouse neural stem cell nucleofector kit (Lonza).
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bioRxiv - Cell Biology 2020Quote: ... Tln1flox/flox (control) and Tln1flox/flox Tln2−/− mouse kidney fibroblasts36 were cultured in DMEM–F12 (12-719, Lonza) with 10% FBS and 100 U/ml penicillin–streptomycin at 37°C ...
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bioRxiv - Immunology 2023Quote: Electroporation was performed to introduce siRNA into BMDCs using a mouse dendritic cell nucleofector kit (Lonza, Basel, Switzerland) and a Nucleofector 2b (Lonza) ...
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bioRxiv - Immunology 2021Quote: ... Staining with primary and secondary antibodies was performed in 50 μl PBS (Lonza) containing 0.5% bovine serum albumin and 0.1% sodium azide (PBA) ...
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bioRxiv - Physiology 2023Quote: ... RNASelect dye and antibodies were suspended in 0.2 µm-filtered DPBS (Lonza, USA) and centrifuged at 21 000 × g for 20 min at 4°C to eliminate potential debris and aggregates ...
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bioRxiv - Cancer Biology 2019Quote: ... NSCs were harvested with Accutase and 2-3×106 cells were resuspended in 100µl mouse neural stem cell nucleofector buffer (Lonza) with 2µg plasmid DNA ...
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bioRxiv - Developmental Biology 2021Quote: ... pPBCAG-hph and a PiggyBac Transposase vector using the AMAXA Mouse Embryonic Stem Cell Nucleofector Kit (LONZA, VPH-1001). Transfected cells were selected with 200 μg/ml hygromycin B Gold (Ibian tech. ...
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bioRxiv - Developmental Biology 2021Quote: ... 5×106 EL16.7 TST ESCs were nucleofected with the AMAXA Mouse Embryonic Stem Cell Nucleofector Kit (LONZA, VPH-1001) using program A-30 with 1.6 µg each of GFP and tdTomato circularised targeting vectors and 5 µg single gRNA vector PX459 (5’-TATACCTAATCATTATGCCG-3’ ...
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bioRxiv - Cancer Biology 2021Quote: ... The siRNA transfections for primary BMDMs and pancreatic fibroblasts were performed using the Mouse Macrophage Nucleofector™ Kit (Lonza) and Nucleofector™ 2b Device (Lonza ...
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bioRxiv - Biochemistry 2021Quote: ... or ds-UM DNA oligos (IDT) (Table S4) were nucleofected into mouse ES cells using an Amaxa nucleofector (Lonza) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... For each nucleofection 3 x 106 cells were resuspended in 80 μl of mouse ES cell nucleofection solution (Lonza). Equimolar (0.32 nmol ...
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bioRxiv - Molecular Biology 2019Quote: ... and wild-type J1 male mouse embryonic stem cells were grown on 0.1% gelatin-coated dishes in DMEM (Lonza) complemented with 10% (v/v ...
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bioRxiv - Immunology 2021Quote: ... On day 7 cells were nucleofected using the Amaxa Mouse T Cell Nucleofector Kit (Lonza, Cat no. VVPA-1006) per the manufacturer’s instructions with 5x106 cells and 1.5µg plasmid DNA per cuvette ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs were immortalized with pBabe-SV40Tag by electroporation using the Amaxa Mouse/Rat Hepatocyte Nucleofector Kit (Lonza, VPL-1004). Immortalized MEF lines were genotyped ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse CCL206 lung fibroblasts (CCL-206; ATCC, Wesel, Germany, RRID: CVCL_0437) were cultured in DMEM (Lonza, Basel, Switzerland, #42430):Ham’s F12 (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: All cell lines were authenticated by Brca1/2-specific PCR-based genotyping (mouse)7,8 and they were regularly tested for mycoplasma contamination (Mycoalert, Lonza).
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bioRxiv - Cell Biology 2023Quote: ... Ki-67 depletion was performed by nucleofection with an siRNA against mouse Ki-67 (CGUUGAUAUCAGCAACUUU) using Amaxa Nucleofector (Lonza), in accordance with the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... A total of 500,000 cells were transfected using the using the mouse neural stem cell nucleofection kit (Lonza, VPG-1004) with 0.66 μg of the construct and 0.33 μg of transposase using the Lonza Amaxa Nucleofector I with protocol A-33 ...
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bioRxiv - Neuroscience 2019Quote: ... Ganglionic eminences and cortex were dissected and dissociated cortical neurons were nucleofected with ON-TARGET plus mouse Mecp2 siRNA or Non-targeting siRNA 1 (Dharmacon) according to the protocol of Amaxa Nucleofection (Lonza). Then neurons were plated into microchambers coated with poly-D-lysine (0.1 mg/ml ...
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bioRxiv - Cancer Biology 2019Quote: 1-4 × 106 dissociated cells were re-suspended in 100 µL of Amaxa Mouse NSC Nucleofector Solution (VPG-1004, Lonza) with 5 µg of plasmid DNA ...
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bioRxiv - Neuroscience 2021Quote: ... the cell suspension was transfected with a Homer 1c::TdimerDsRed plasmid (Bats et al., 2007) by electroporation using the Amaxa Nucleofector 2b device and Amaxa mouse neuron nucleofector kit (Lonza). Neurons were then plated on poly-L-lysine-coated glass coverslips and grown at 37°C in a humidified 5% CO2-containing atmosphere in BME supplemented with KCl (25 mM final concentration) ...
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bioRxiv - Immunology 2020Quote: ... Rinsed cells were resuspended in buffer P4 (mouse) or P2 (human) at 1-10 x 106 cells/20 μL (Lonza). Non-targeting Alt-R crRNA #1 ...
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bioRxiv - Microbiology 2022Quote: ... Blood schizont purification was performed using a Nycodenz (Axis Shield) gradient following the culture of infected mouse blood in RPMI 1640 (Lonza) supplemented with 50 µg/ml neomycin (Sigma ...
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bioRxiv - Immunology 2022Quote: ... Knockdown of Vps4a/b was performed using Amaxa Nucleofector II and the Mouse T-Cell Nucleofector Kit (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Nucleofection was performed on an Amaxa nucleofector IIb using protocol O-003 with the mouse neuron kit (Lonza, VPG-1001). Cells were allowed to recover for 1 hour at 37 °C and followed by NCAM1 MACS and culture as described above.
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bioRxiv - Cell Biology 2021Quote: ... was generated by the introduction of a plasmid carrying the EGFP gene under the control of the CAG promoter to EStTA5-4 cells using the Amaxa Mouse ES cells Nucleofector Kit (VPH-1001, Lonza).
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bioRxiv - Pathology 2020Quote: ... Male transgenic uPA+/+/SCID mice were transplanted as previously described.33 The following PHH donors were used: cryopreserved or mouse-passaged (mp)PHH1 (HUM4188, Lonza), cryopreserved or mpPHH2 (HUM4129 ...
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bioRxiv - Cell Biology 2020Quote: ... 10 µg of plasmids were mixed with 5 millions ESC in buffer “Mouse ES cell nucleofector kit (Lonza, VPH-1001) per electroporation ...
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bioRxiv - Genetics 2021Quote: ... first excised floxed Neomycin resistance cassette from Oct4 (CiA:Oct4) mESCs 12 by transfecting Cre-mCherry plasmid using Mouse Embryonic Stem Cell Nucleofector™ Kit from Lonza. After 24 hours after transfection ...
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bioRxiv - Genetics 2021Quote: ... Cas9-sgRNA-Thy1.1 plasmid transfection was carried out by electroporation using Mouse Embryonic Stem Cell Nucleofector™ Kit from Lonza. After 36 hours of transfection Thy1.1 expressing cells were FACS sorted and sparsely seeded for clonal expansion on 15cm plate ...
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bioRxiv - Immunology 2021Quote: ... Naïve T cells expressing Cas9 were washed once with 1 mL pre-warmed recovery medium (Mouse T Cell Nucleofector Medium, Lonza) before electroporation ...
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bioRxiv - Cell Biology 2021Quote: ... mouse embryonic fibroblasts (MEFs) or African green monkey kidney fibroblasts (COS7) were cultured (37°C, 5% CO2) in DMEM (Lonza), supplemented with 10% fetal bovine serum (Life Technologies) ...
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bioRxiv - Immunology 2022Quote: ... was prepared by mixing 250 nmol target gene sgRNA+ 50 nmol eGFP sgRNA+ 50 nmol SpCas9 2NLS Nuclease (Synthego) in 50 μL mouse B cell nucleofection buffer (Lonza) at RT for 30min ...
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bioRxiv - Neuroscience 2023Quote: Transfection of dissociated neurons was performed using the AMAXA Nucleofector system (program O.005) with the mouse neuron Nucleofector kit (Lonza) following the manufacturer’s specifications ...
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bioRxiv - Immunology 2023Quote: Hepatocytes (7×105 cells) were transfected with various plasmids (at 6 μM or indicated concentrations) using the Mouse/ Rat hepatocyte Nucleofector kit (Lonza) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were nucleofected with 4 µg of the sgRNA-containing plasmid individually following the Amaxa Mouse ES cell Nucleofector kit recommendations (VPH-1001, Lonza). Later ...