Labshake search
Citations for Lonza :
251 - 300 of 343 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... were cultured at 27°C in Insect-XPRESS protein-free insect medium with L-Glutamine (Lonza) in shaking suspension ...
-
bioRxiv - Microbiology 2023Quote: ... in infection medium (Eagles’ Minimum Essential Medium (MEM) with L-glutamine (Lonza, Cat. No. BE12-611F) containing 2x antibiotic-antimycotic (Gibco ...
-
bioRxiv - Cell Biology 2023Quote: ... Human SH-SY5Y dopaminergic neuroblastoma cells were cultured in Dulbecco’s modified Eagle’s medium:F-12 (DMEM:F-12; 1:1) containing high glucose (3.151 g/L) (Lonza), supplemented with 10% (v/v ...
-
bioRxiv - Developmental Biology 2023Quote: ... using the Amaxa™ P3 Primary Cell 4D-Nucleofector™ X Kit L (Lonza, #V4XP-3024), in 100μl nucleofection buffer according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: Cryopreserved PBMC (5 × 106/sample) were thawed in prewarmed RPMI-1640 with L-glutamine (Lonza, Basel, Switzerland) + 10% FCS ...
-
bioRxiv - Genomics 2019Quote: ... TetO-CDC6 mESCs were cultured on 0.1% gelatin-coated plates in Dulbecco’s modified Eagle’s medium (DMEM) with Ultraglutamine 1 and 4.5 g/L glucose (Lonza) supplemented with 15% FBS (Sigma) ...
-
bioRxiv - Immunology 2019Quote: ... Hybridoma cells were electroporated using the SF Cell Line 4D-Nucleofector X Kit L (Lonza, V4XC-2024) with the program CQ-104 ...
-
bioRxiv - Cancer Biology 2022Quote: Nucleofection experiments of ALL-B cells were carried out using Amaxa 4D-Nucleofector X kit L (Lonza) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: Spinner adapted murine L929 (L) cells were grown at 37°C in Joklik’s minimal essential medium (Lonza) supplemented with 5% fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... LNCaP-ABL and LNAI cells were cultured in RPMI 1640 with L-glutamine (Lonza, Switzerland #12-702Q) supplemented with 10% charcoal-stripped fetal bovine serum (Biowest ...
-
bioRxiv - Molecular Biology 2023Quote: ... using the CRISPR/Cas9 system and the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza, Switzerland). Oligonucleotides for gRNA were selected by Сrispor (Tefor Infrastructure ...
-
bioRxiv - Immunology 2024Quote: ... Wien-133 (human CD52-positive Burkitt’s lymphoma) cells were cultured in Iscove’s Modified Dulbeco’s Medium (IMDM) with HEPES and L-Glutamine (Lonza), supplemented with 10% (v/v ...
-
bioRxiv - Cell Biology 2024Quote: ... HMVEC-L were cultured in EGMTM-2MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM (Lonza, CC-3202) at 37°C ...
-
bioRxiv - Synthetic Biology 2019Quote: ... using the SF Cell Line 4D-Nucleofector® × Kit L or × Kit S (Lonza, V4XC-2024, V4XC-2032) with the program CQ-104 ...
-
bioRxiv - Microbiology 2021Quote: ... and 2mM L-glutamine. Spinner-adapted L929 cells (obtained from T. Dermody’s laboratory) were maintained in Joklik’s MEM (Lonza) supplemented to contain 5% FBS ...
-
bioRxiv - Microbiology 2019Quote: ... and 2 mM L-glutamine. Spinner-adapted L929 cells (obtained from T. Dermody’s laboratory) were maintained in Joklik’s MEM (Lonza) supplemented to contain 5% FBS ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... D Drucker) were routinely cultured in Dulbecco’s modified eagle medium (DMEM) (5.5 mmol/L D-glucose) (Lonza, UK), supplemented with 10% (v/v ...
-
bioRxiv - Immunology 2019Quote: ... To image T cells migrating randomly on immobilized ICAM-1 cells were resuspended in L-15 media (Lonza) supplemented with 6mM Glucose and allowed to adhere to ICAM-1 coated chambers ...
-
bioRxiv - Cell Biology 2021Quote: ... was cultured in Corning Medium F-12K with L-glutamine (BioWhittaker® Medium F12K Medium, Lonza, Maryland, USA), and 10% fetal calf serum ...
-
bioRxiv - Cell Biology 2020Quote: Jurkat T cells (clone E6.1; ATCC TIB-152) were grown in RPMI 1640 medium w/ L-Glutamine (Lonza) supplemented with 10% Fetal Bovine Serum and 1% penicillin/streptomycin ...
-
bioRxiv - Cancer Biology 2022Quote: Culture medium tested in this study was E-media prepared by adding 1:1 ratio of Iscove’s Modified Dulbecco’s Medium (IMDM) with HEPES without L-Glutamine (purchased from Lonza), and Dulbecco’s Modified Eagle Medium (EMDM)/F12 1:1 with 1.0 g/L glucose ...
-
bioRxiv - Cell Biology 2022Quote: ... BUB-1 and CLS-2::GFP production was performed in 2 L SF9 cells in Insect-XPRESS (Lonza) medium (1×10^6 cells/mL) ...
-
bioRxiv - Immunology 2022Quote: ... used for baculovirus preparation were maintained in Insect-XPRESSTM Protein-free Insect Cell Medium with L-Glutamine (Lonza) at 28°C under agitation ...
-
bioRxiv - Synthetic Biology 2024Quote: ... using SF Cell line 4D-Nucleofector X Kit L or X Kit S (Lonza, V4XC-2024, V4XC-2032) with the program CQ-104 ...
-
bioRxiv - Molecular Biology 2021Quote: ... siRNA transfections were performed using the 4D-Nucleofector and the SE cell line 4D-Nucleofector X kit L (Lonza) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2021Quote: ... Cells from the FaDu cell line (a hypopharyngeal cancer cell line gifted by Dr Craig Murdoch (Sheffield University)) were cultured in complete medium (Dulbecco’s minimum essential medium Eagle with L-glutamine (DMEM) (Lonza) + 10% fetal bovine serum (Invitrogen) ...
-
bioRxiv - Genetics 2020Quote: ... 5μg vector (in 5μl) transfected into 5 million CD4 T cells in 100μl 1M nucleofection solution67) using a Nucleofector 2b device (Lonza; program V024 for resting T cells and T023 for stimulated T cells) ...
-
bioRxiv - Immunology 2019Quote: ... Pre-activated B cells were transfected using the P4 Primary Cell 4D-Nucleofector X Kit L (Lonza, V4XP-4024) in combination with the program DI-100 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1,000,000 cells were nucleofected with 3.33 µg of SQT1665 and 1.66 µg of plasmid expressing gRNA using SE Cell Line 4D-NucleofectorTM X Kit L (Lonza) according to manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... using SF Cell line 4D-Nucleofector® X Kit L or X Kit S (Lonza, V4XC-2024, V4XC-2032) with the program CQ-104 ...
-
bioRxiv - Bioengineering 2022Quote: ... embryos were anesthetized by 30 mg/L tricaine-S (Western Chemical) and mounted in 2% low melting agarose (LONZA) for dorsal or lateral view in 35 mm Petri dishes with a glass bottom ...
-
bioRxiv - Cell Biology 2022Quote: ... OCI-LY-1 cells were transfected using the Amaxa® Cell Line Nucleofector® Kit L (Lonza, Basel, Switzerland), program C-05 as previously described(46) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 33 μL cell suspension was mixed with 66 μL of solution 1 (0.83% low-melting-point agarose SeaPlaque GTG (Lonza), 170 mM sorbitol ...
-
bioRxiv - Microbiology 2021Quote: ... were electroporated with 20 ug of guide plasmid and 60 ug of linearized repair plasmid using an Amaxa 4D electroporator and P3 Primary cell 4D Nucleofector X Kit L (Lonza) using programme FP158 as described (Collins et al. ...
-
bioRxiv - Immunology 2022Quote: ... a total of 1.6 x 108 cells were transfected with 2 μM of the corresponding ASO using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza) and the EO-115 program ...
-
bioRxiv - Cell Biology 2022Quote: ... 90 μL of nucleofection solution (16.2 μL of Supplement solution mixed with 73.8 μL of SF solution from SF Cell Line 4D-Nucleofector™ X Kit L) (Lonza) was mixed thoroughly with the cell pellet ...
-
bioRxiv - Neuroscience 2019Quote: ... the neurons were resuspended in DMEM/F12 and electroporated using a P3 Primary Cell 4D-Nucleofector X Kit L (cat. no. V4XP-3012, Lonza) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... the transfected cells were co-cultured with BHK-21 cells (ATCC CCL-10) transfected with VSV-G using the SE cell Line 4D-Nucleofector X Kit L (Lonza) in DMEM without glutamine (Life Technologies) ...
-
bioRxiv - Biochemistry 2020Quote: ... COS7 cells were cultured in DMEM with 4.5g/l glucose supplemented with 10% DMEM and antibiotics (Lonza Biologics, Slough, UK). transiently transfected with expression plasmids using TransIT-2020 (Mirus ...
-
bioRxiv - Microbiology 2021Quote: ... Both resuspensions were mixed in a 100 μl cuvette (P3 Primary cells 4D-Nucleofector X kit L, V4XP-3024, Lonza). The programme FI-158 was used for electroporation ...
-
bioRxiv - Biochemistry 2022Quote: ... and 18-36 μl ribonucleoprotein complexes were added along with 4 μM Alt-R Cas9 electroporation enhancer (IDT) to a nucleofection chamber (Lonza). Cells were electroporated using the nucleofector program EN-138 and gently resuspended in DMEM ...
-
bioRxiv - Cell Biology 2022Quote: ... we used Cas9-RNP and the Lonza 4D-Nucleofector System together with the P4 Primary Cell 4D-Nucleofector X Kit L (Lonza) to transfect and create Ndrg2-KO DCs ...
-
bioRxiv - Cell Biology 2022Quote: ... For nucleofection we used the 4D-Nucleofector System and P3 Primary Cell 4D Nucleofector X Kit L (Lonza, Basel, Switzerland). We optimized the RNP electroporation for DCs by using multiple-guide mixtures and different number of cells in a 100uL total volume ...
-
bioRxiv - Molecular Biology 2020Quote: ... Gel electrophoresis after visual read-out of the LAMP assay was done by loading 5 µl of the lamp reaction with 5 µl 2x loading dye on a 1.5 % agarose (Seakem LE Agarose, Lonza #50004) together with 5 µl of a 1kB DNA Ladder (Roche) ...
-
bioRxiv - Immunology 2020Quote: ... HIV-1-infected subjects and multiple myeloma patients were thawed at 37°C and washed twice with RPMI 1640 medium with L-Glutamine (Lonza). Then ...
-
bioRxiv - Genetics 2020Quote: ... hESCs were nucleofected with 2 μg of CRISPR and 3 μl ssODN (100 μm) using the P3 Primary Cell Kit L (Lonza). hESCs were then plated onto puromycin-resistant (DR4 ...
-
bioRxiv - Microbiology 2021Quote: ... Tightly synchronized schizonts were transfected using the Amaxa 4-D electroporator and P3 Primary Cell 4D Nucleofector X Kit L (Lonza) according to the protocol described by Moon et al ...
-
bioRxiv - Immunology 2020Quote: Targeted gene’s expression was knocked-down in up to 5 x 106 cells using the P3 Primary Cell 4D-Nucleofector X Kit L (V4XP-3024, Lonza) with 90 µl P3 Primary cell solution and 100 pmol of corresponding si_RNA (resuspended in 10 ul RNAse-free H2O) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2×107 epimastigotes were resuspended in 100μl of Tb-BSF buffer (Schumann Burkard et al., 2011) and submitted to the program U033 in AMAXA Nucleofector (Lonza). Twenty-four hours after transfection ...
-
bioRxiv - Immunology 2021Quote: ... according to the manufacturer instructions and seeded at 5×105 cells/mL in RPMI-1640 media with L-glutamine (Lonza) supplemented with 10% fetal bovine serum (FBS ...