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Citations for Lonza :
51 - 100 of 1021 citations for HLA A*0201 HPV16 E7 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Human neonatal keratinocytes (Lonza) were grown according to manufacturer’s recommendations ...
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bioRxiv - Physiology 2020Quote: ... Normal human astrocytes (Lonza) were cultured in astocytic basal medium (ABM ...
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bioRxiv - Neuroscience 2022Quote: Primary human astrocytes (Lonza) were maintained in a complete astrocyte growth medium (ScienCell ...
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bioRxiv - Neuroscience 2022Quote: Primary human astrocytes (Lonza) were maintained in complete astrocyte growth medium (ScienCell ...
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bioRxiv - Bioengineering 2023Quote: Human BMSC (Lonza, 19TL155677) were thawed and plated at 6000 cells/cm2 in an expansion culture medium containing a basal alpha minimum essential medium (α-MEM,22571) ...
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bioRxiv - Developmental Biology 2023Quote: Human aortic SMCs (Lonza) were cultured up to passage 6 in M199 medium supplemented with 10% FBS and growth factors (PeproTech ...
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bioRxiv - Bioengineering 2023Quote: ... Human lung fibroblasts (Lonza) at passage 7 were maintained in FibroLife S2 Fibroblast Medium (Lifeline Cell Technology ...
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bioRxiv - Neuroscience 2024Quote: Primary human astrocytes (Lonza) were cultured in a complete astrocyte growth medium (ScienCell ...
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bioRxiv - Neuroscience 2024Quote: Fetal human astrocytes (Lonza) were cultured in Astrocyte Media (ScienCell ...
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bioRxiv - Molecular Biology 2020Quote: ... 1×106 EBdCas9 cells were transfected with the above RNP complex and 100μM of ssDNA (HDR)(Sup Table 8) using AMAXA electroporation kit (LONZA) and plated in TeSR media (supplemented with 5μM ROCKi) ...
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bioRxiv - Cancer Biology 2023Quote: ... 3uL of the total Cas9-RNP complex was added to 200,000 K562 cells resuspended in 18uL SF cell solution (Lonza) and subjected to nucleofection with the Lonza 4D nucleofectorsystem using manufactured specified conditions for K562 cells ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were washed twice with PBS and 2 × 106 cells were resuspended in 100 μl of Solution V (Lonza) and then mixed with 5 μg purified sgRNA expression vector or the empty vector as a negative control before nucleofection using program Q-001 of a Nucleofector™ 2b (Lonza) ...
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bioRxiv - Bioengineering 2020Quote: Human MSCs (hMSCs) were isolated from fresh unprocessed bone marrow from human donors (Lonza) as previously described 63 ...
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bioRxiv - Biophysics 2019Quote: The HEK293 adherent culture cells were transfected with pcDNA3.4-PCV2d plasmid using Amaxa Nucleofector II instrument by Lonza (program A-23). The transfected cell were plated on 6-well plate ...
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bioRxiv - Immunology 2022Quote: ... IFNAR KO A549 cells were generated by CRISPR-Cas9 ribonucleoprotein (RNP) complex (IDT) transfection using the Nucleofector system (Lonza Bioscience). Specifically ...
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bioRxiv - Genetics 2022Quote: ... Cells were dissociated using accutase and 1 x 106 cells in P3 buffer were mixed with the RNP/template complex and nucleofected in large cuvettes (V4XP-3024 Lonza) using 4D-Nucleofector on program CA137 ...
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bioRxiv - Immunology 2022Quote: ... containing RNP complex and enhancer DNA (IDT, Cat.1075915) and under electroporation with EO-115 program of 4D-nucleofactor X Unit (Lonza). Electroporated T cells recovered overnight in IL7 and Il15 medium were then activated by anti-human CD3/CD28 beads (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 pmol of the dsODN was mixed together with 100 µl homemade nucleofection solution to the RNP complex and electroporated using Nucleofector 2b (Lonza) with A23 program and 2 mm electroporation cuvettes.
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bioRxiv - Immunology 2022Quote: ... 4.4 μl gRNA-Cas9 complex was added to a 20 μl cell suspension in P3 buffer and transferred into a nucleovette (Lonza), placed on Amaxa Nucleofector and 96 well Shuttle (Lonza ...
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bioRxiv - Immunology 2022Quote: ... containing RNP complex and enhancer DNA (IDT, Cat.1075915) and under electroporation with CM-137 program of 4D-nucleofactor X Unit (Lonza). Electroporated T cells recovered overnight in IL7 medium were then activated by anti-mouse CD3/CD28 beads (at 1:1 ratio for two days and expanded in culture medium containing 5ng/ml hIL2 ...
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bioRxiv - Immunology 2023Quote: CRISPR–Cas9 gene knockout was performed by transient Cas9/gRNA (RNP) complex electroporation using the P3 Primary Cell 4D-Nucleofector X Kit S (Lonza). On day 4 of culture ...
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bioRxiv - Genomics 2023Quote: ... 2.5e5 RPE1 CRISPRi cells were nucleofected with 3μM RNP complex using an SE Cell Line 4D X Kit S (Lonza Bioscience) on a 4D-Nucleofector (Lonza Bioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... were resuspended in 4D-Nucleofector Solution and then mixed with the Cas9/sgRNA complex and electroporated using the 4D-Nucleofector X Unit (Lonza). All tumor cell lines were maintained in high-glucose DMEM supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: CRISPR-mediated mutagenesis was carried out by transiently introducing CRISPR/Cas9 RNA-nucleoprotein complex into cultured NB4 cells with the 4D-Nucleofector system (Lonza). The sample preparation kit was Lonza SF Cell Line 4D-Nucleofector Kit S (Cat# V4XC-2024) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected with RNP complex and HDR templates by nucleofection with SF Cell Line 4D-Nucleofector X Kit (Lonza) using 20-ml Nucleocuvette Strips ...
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bioRxiv - Microbiology 2023Quote: ... or 1×106 CEM-M7 Cas9 cells were transfected with the HiFi Cas9 Nuclease V3 (IDT)/gRNA complex (80 pmol/300 pmol) (Lonza) using a non-targeting or a GRN(5’-GCGATCCTGCTTCCAAAGATC-3’) ...
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bioRxiv - Immunology 2021Quote: Human normal dendritic cells (Lonza) were cultured in 50 ng/ml GM-CSF and 50 ng/ml IL-4 for 5 days ...
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bioRxiv - Bioengineering 2021Quote: Cryopreserved primary human hepatocytes (Lonza) were thawed according to manufacturer’s protocol and the cells were directly used in experiments ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Normal human lung fibroblasts (Lonza) and Primary Human IPF Lung Parenchymal Fibroblasts (Donor2 ...
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bioRxiv - Bioengineering 2019Quote: ... Human BM CD34+ cells (Lonza) were expanded for 5 days in Stemline II (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Normal human astrocytes (NHA)(Lonza) were thawed at P5 and cultured for 5-10 days prior to cell seeding ...
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bioRxiv - Bioengineering 2023Quote: Human bone marrow aspirates (Lonza) were purchased to isolate primary hMSCs ...
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bioRxiv - Bioengineering 2023Quote: Primary human hepatocytes (HUCPG, Lonza) were cultured following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Human Intestinal Myofibroblasts (HIMF) (Lonza) were trypsinized ...
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bioRxiv - Microbiology 2024Quote: ... normal human bronchial epithelial (Lonza), and porcine primary nasal epithelial (24 ...
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bioRxiv - Bioengineering 2024Quote: ... and recombinant human insulin 0.5%) (Lonza). Human cervical cancer cell line SiHa from ATCC (ATCC® HTB-35™ ...
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bioRxiv - Biophysics 2024Quote: Human umbilical vein ECs (Lonza) were cultured using standard protocols in Endothelial Growth Medium (EGM2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Untransformed Normal Human Astrocytes (NHA) and Human Neural progenitor cells (NPC) were purchased from Lonza Bioscience and maintained using respective basal medium supplemented with growth factors (Lonza Biosciences) ...
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bioRxiv - Bioengineering 2021Quote: Human bone marrow mesenchymal stromal cells (hBMSCs) were isolated from human bone marrow (Lonza, USA) and characterized as previously described [32] ...
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bioRxiv - Cancer Biology 2019Quote: ... Normal human osteoblasts (NHOs) and primary human umbilical vein cells (HUVECs) were purchased from Lonza. All cell lines were grown and maintained in accordance with standard cell culture techniques ...
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bioRxiv - Neuroscience 2019Quote: ... Co-transfection of 500 ng sgRNA plasmid and 1 µl of 10 µM ssODN template into 2×105 HEK293 cells was performed using nucleofection on the 4D-Nucleofector system (LONZA, Basel, Switzerland), as described previously38 ...
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bioRxiv - Cancer Biology 2022Quote: ... and mixed by pipetting with the full gRNA-Cas9 complex mix previously assembled then added in the electroporation chamber well (Lonza, V4XP3032). Cells were electroporated with the program EO-100 using the Lonza Nucleofector ...
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bioRxiv - Systems Biology 2024Quote: ... MT2 and MDA-MB-231 cells with HLA-A,B,C CRISPR-Cas9 knockout were generated through electroporation of Cas9-sgRNA ribonucleoprotein (RNP) complex using 4D-Nucleofector (Lonza Bioscience). Three sgRNAs targeting HLA gene (HLA sgRNA#1 ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... MDA-MB-231 and PC3 cells with FBXO22 CRISPR-Cas9 knockout were generated through electroporation of Cas9-sgRNA ribonucleoprotein (RNP) complex using 4D-Nucleofector (Lonza Bioscience). Two sgRNAs targeting FBXO22 gene (FBXO22 sgRNA#1 ...
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bioRxiv - Microbiology 2020Quote: ... Human embryonic lung fibroblasts MRC-5 and human melanoma MeWo cells were cultured in DMEM (Lonza), supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2021Quote: Human umbilical vein endothelial cells (HUVEC) and normal human lung fibroblasts (HLF) were purchased from Lonza and cultured in EGM-2MV and FGM-2 ...
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bioRxiv - Bioengineering 2021Quote: ... Human umbilical vein endothelial cells (HUVECs) and normal human lung fibroblasts (NHLFs) were purchased from Lonza and cultured in EGM-2 and FGM-2 (Lonza ...
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bioRxiv - Cell Biology 2020Quote: ... cells were resuspended in 100 μl Human MSC Nucleofector Solution (Human MSC Nucleofector® Kit - Lonza) and the transfection was carried out according to the manufacturer’s instructions using different amount of gRNA plasmids or pmaxGFP plasmid ...
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bioRxiv - Bioengineering 2024Quote: ... Human microvascular endothelial cells (hMVEC) and human umbilical vein endothelial cells (hUVEC) were obtained from Lonza (hMVEC CC-2543 ...
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bioRxiv - Cell Biology 2020Quote: ... Human DPSCs were obtained from Lonza Walkersville ...