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Citations for Lonza :
1 - 50 of 127 citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... sperm fraction was diluted 1:10 in Phosphate Buffered Saline (PBS, Lonza) and CellROX reagents (1 μM/μl ...
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bioRxiv - Developmental Biology 2021Quote: ... AnnexinV reagent was added to sperm fraction diluted 1:10 in PBS (LONZA) and the solution incubated on ice for 15 minutes ...
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bioRxiv - Biophysics 2022Quote: ... Fish were subsequently transferred into 1.5% low-melt agarose (Lonza, 50100), and transferred to a clear dish suspended in low-melt agarose for imaging ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA in the libraries were visualized using the FlashGelTM DNA Cassette (Lonza, 57032) together with the Gel Loading Buffer II (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 µg donor DNA and 10 µg Cas12a crRNA template DNA were diluted in either 20 µl (Lonza transfection) or 50 µl (Tb-BSF transfection ...
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bioRxiv - Genomics 2023Quote: ... Neurons were nucleofected with 10 µ total DNA plasmid DNA using the AD1 4D-Nucleofector Y kit (Lonza V4YP-1A24) with the ED158 (iCell GlutaNeurons ...
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bioRxiv - Cell Biology 2023Quote: ... 4 μM Alt-R Cas9 Electroporation enhancer (IDT) and 4 μM single-strand DNA homology template (Ultramer DNA Oligonucleotide, Lonza). After electroporation ...
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bioRxiv - Microbiology 2024Quote: ... with the FlashGelTM DNA Marker Ladder (Lonza, 50473) for size confirmation ...
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bioRxiv - Microbiology 2020Quote: ... DNA yield and quality was assessed by electrophoresis using a 0.75% agarose gel containing the DNA stain GelStar (Lonza, Rockland, ME, USA) in 1X Tris Borate EDTA (TBE ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was imaged by staining with SYBR green (LONZA). All visual bands as well as the bands at the same locations in the other lanes were excised from the gel ...
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bioRxiv - Cell Biology 2023Quote: ... Neurons were transfected with plasmid DNA using Nucleofector (Lonza) before plating as described (Minegishi et al. ...
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bioRxiv - Neuroscience 2020Quote: ... we used 2.2 % agarose gel electrophoresis (FlashGel DNA System, Lonza) and Bioanalyzer High-Sensitivity DNA Assay (Supplementary Figure 2) ...
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bioRxiv - Systems Biology 2020Quote: ... Sheared DNA was run on a 1% agarose gel (Lonza) to verify that sheared DNA was between 100-1000 bp ...
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bioRxiv - Immunology 2023Quote: The 3 kbp DNA plasmid encoding green fluorescent protein (GFP) (Lonza) was used to assess transfection efficiency ...
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bioRxiv - Molecular Biology 2021Quote: ... phosphodiester linkages in the DNA backbone were cleaved by DNAse I (Lonza).
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bioRxiv - Developmental Biology 2019Quote: ... The DNA constructs were introduced into the cells with Amaxa Nucleofector (Lonza).
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bioRxiv - Molecular Biology 2024Quote: ... DNA samples were loaded on 3% NusieveTM GTGTM Agarose gel (Lonza, 50081), and electrophoresis was run at 100V in 1X TBE buffer for ∼50 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified DNA was separated on a 3% NuSieve GTG agarose gel (Lonza #50081). The gel band corresponding to the size of dinucleosomal DNA (∼250 to 350 bp ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was separated on a 3% NuSieve GTG agarose gel (Lonza #50081). The gel band corresponding to the size of dinucleosomal DNA (∼250 to 350bp ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with ~5 μg plasmid DNA using nucleofection (Amaxa, Lonza AG) according to manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: DNA was delivered by nucleofection using the Nucleofector 2b system (Lonza VPH-1001). 1.5 million A17iCre mESCs were plated on gelatin-coated 10 cm dishes 2 days before delivery ...
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bioRxiv - Genomics 2020Quote: ... The first consisted of 2 μl/well pmaxGFP DNA (LONZA,1 μg/ul) diluted in 250 μl/well Opti-MEM® medium (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA from supernatants and pellets were analyzed on 1% agarose (Lonza cat. # 50000) gels in TAE buffer at 3 V/cm for 40 min ...
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bioRxiv - Molecular Biology 2023Quote: ... was resuspended in 110 µl P3+DNA solution (Lonza Cat. No. V4Xp-3024). Transfection of either free merozoites carried out in a 4D-Nucleofector System (Lonza) ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were transfected with 2.26µg total plasmid DNA in SF buffer (Lonza, V4SC-2096), using program CM134 and incubated for 24 hours prior to luminescence measurement ...
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bioRxiv - Biophysics 2022Quote: CH27 cells were transiently transfected with plasmid DNA encoding membrane anchor constructs by Lonza Nucleofector electroporation (Lonza ...
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bioRxiv - Cell Biology 2022Quote: ... The cell/DNA mixture was then nucleofected using the 4-D nucleofector system (Lonza) code CB-150 and densely replated on Matrigel coated 6-well plates at 2.5×106 cells per well ...
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bioRxiv - Microbiology 2021Quote: ... Parasites were transfected with 50-100 µg DNA using either the Amaxa system (Lonza) or the Biorad system as previously described (Moon et al. ...
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bioRxiv - Cell Biology 2023Quote: ... and the 50 bp – 1.5 Kb FlashGel™ DNA Marker as ladder (Lonza, 57033). The 2100 Bioanalyzer of Automated Droplet Generator (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... All PCR products and double-stranded DNAs were purified with agarose gels (Lonza, 50004) and the GeneJet Gel Extraction Kit (Thermo Fisher ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... 1 μg plasmid DNA was added and cells electroporated in a 4D-NucleofectorTM System (Lonza) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... plasmid DNA (50 µg per pool) was resuspended in 100 µl of buffer P3 (Lonza) with 4 µl ATP (625 mM) ...
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bioRxiv - Molecular Biology 2020Quote: ... HeLa cells were transfected with the gRNAs and ss donor DNA using a nucleofector (Lonza, solution SE ...
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bioRxiv - Microbiology 2024Quote: ... The DNA constructs in TE buffer were mixed with 90µl P3 Primary cell solution (Lonza) and used to resuspend 20µl segmented schizonts which were subsequently transferred to a transfection cuvette ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were electroporated with the corresponding DNA vector using a 4D-Nucleofector (Lonza, program DS-112) according to protocol of manufacturer and re-plated in plastic dish (Costar ...
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bioRxiv - Microbiology 2020Quote: ... 25 μg linearized DNA were added to the parasites and then nucleofected using 4D-NucleofectorTM (Lonza) with setting EO-115 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Horizontal electrophoresis of DNA was carried out using 1% Seakem LE agarose gels (Lonza, Basel, Switzerland). All cloning and molecular biology experiments were carried out according to established protocols ...
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bioRxiv - Biophysics 2022Quote: ... Jurkat cells were electroporated with 1μg of DNA plasmid pcDNA3.1_mLck_GFP (produced in the lab, AM Lellouch) with Nucleofector 2b device (Lonza), and selected by antibiotic G418 ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were transfected with a maximum of 0.6 μg total plasmid DNA using a Nucleofector (Lonza) and following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Dinucleosome-sized DNA fragments were purified and excised from a 3% NuSieve GTG agarose gel (Lonza) using the Zymoclean Gel DNA Recovery Kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2024Quote: ... containing 10 µg of Cas9 or Cas9/guide plasmidic DNA and transferred to a cuvette (Lonza). Cells were nucleofected with the DT-130 program using the 4D-Nucleofector system with Core and X unit (Lonza) ...
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Ultra-high-throughput microbial single-cell whole genome sequencing for genome-resolved metagenomicsbioRxiv - Microbiology 2022Quote: ... The supernatant solution was collected and the DNA library was confirmed by Flashgel (Lonza, catalog no. 57023), and then purified using a Zymo DNA Clean& Concentrator™-5 kit (Zymo Research ...
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bioRxiv - Neuroscience 2022Quote: ... and 10 μg plasmid DNA in a volume of 25-30 μL of AD1 Solution (Lonza Bioscience) was put as a drop on the slice ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.3) containing 10 μg DNA and transfected by electroporation using an AMAXA Nucleofector® Device (Lonza) with program “X-001 free choice” ...
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bioRxiv - Immunology 2022Quote: ... 2×106 B cells were then nucleofected with 2 μg of plasmid DNA using Nucleofector Kit V (Lonza) and rested for at least 16-24 hours using complete media containing 5 ng/ml BAFF and lacking LPS ...
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bioRxiv - Genomics 2019Quote: ... The DNA fragments of 150–200 bp length were gel-extracted on 3% NuSieve 3:1 Agarose (Lonza). Both samples were sequenced by GATC Biotech AG (Konstanz ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was prepared using ethanol precipitation and electroporation of purified schizonts was carried out using Nucleofactor technology (Lonza). Transfection mixtures were then injected intravenously into a mouse ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA substrates were separated by gel electrophoresis using 1% SeaKem LE Agarose gel (Lonza Group AG, Basel, Switzerland). The bands were excised and purified using QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... program A-30 with 10 µg of plasmid DNA and 1 µg of eGFP control plasmid (Amaxa, Lonza) was used ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 × 106 cells were co-transfected with 5 μg of pSpCas9(BB)– 2A–Puro:sgRNA #4 plasmid and 5 μg of pCSII–CMV:A3B–3×FLAG–IRES–EGFP donor DNA plasmid using the Amaxa Nucleofector (Lonza) with nucleofection solution R ...