Labshake search
Citations for Lonza :
1 - 50 of 137 citations for DNA Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2021Quote: ... were cultured using standard protocols in Endothelial Growth Medium (EGM2; Lonza) and used up to passage 7 ...
-
bioRxiv - Biophysics 2024Quote: ... were cultured using standard protocols in Endothelial Growth Medium (EGM2; Lonza) and used up to passage 7 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Residual endotoxin was assessed by using an Endotoxin Standards kit (Lonza, Anaheim, USA) to ensure there was no LPS contamination in the SEA extracted from control and AChE-KI eggs) ...
-
bioRxiv - Cell Biology 2023Quote: HBE cells were previously established and expanded in standard BEGM Bulletkit (BEGM; Lonza). Passage 1 BEGM-expanded HBE cells were subsequently added to collagen I coated flasks and co-cultured with an equal amount of irradiated NIH/3T3 feeder cells in F-media containing the ROCK inhibitor Y-27632 as described previously(5 ...
-
bioRxiv - Cell Biology 2023Quote: ... All cell lines were routinely monitored for mycoplasma contamination using standard mycoplasma monitoring kits (Lonza).
-
bioRxiv - Microbiology 2021Quote: ... as well as the standard LdBPK_282 cl4 using the Basic Parasite Nucleofector™ Kit 1 (Lonza) with the U-033 program ...
-
bioRxiv - Immunology 2024Quote: ... using standard procedures including red blood cell lysis with ACK lysis buffer (Lonza by Thermo Fisher). Before cell staining ...
-
bioRxiv - Microbiology 2021Quote: ... All cells were kept under standard culture conditions and regularly checked for mycoplasma contamination (MycoAlert Kit, Lonza).
-
bioRxiv - Immunology 2023Quote: ... cells were grown in standard keratinocyte growth medium TM 2 (KGMTM-2) containing 0.15 mM CaCl2 (low Ca– KGM-2; Lonza) without gentamycin ...
-
bioRxiv - Cell Biology 2023Quote: ... The DNA in the libraries were visualized using the FlashGelTM DNA Cassette (Lonza, 57032) together with the Gel Loading Buffer II (Invitrogen ...
-
bioRxiv - Molecular Biology 2020Quote: ... MBP-tagged TRIM21 (pFastBac-MBP-TRIM21) was expressed in SF9 insect cells in Lonza Insect-XPRESS according to standard protocols (Lonza). Cleared cell lysates were prepared by sonication of cell pellets in 50 mM Tris pH 8 ...
-
bioRxiv - Bioengineering 2020Quote: ... were cultured under standard incubation conditions at 37 °C and 5% CO2 in endothelial growth media (EGM BulletKit CC-3124, Lonza). For all imaging experiments ...
-
bioRxiv - Cell Biology 2020Quote: ... The purity of CyaA batches is higher than 90% as judged by SDS PAGE analysis and contained less than 1 EU of LPS/μg of protein as determined by a standard LAL assay (Lonza). Finally ...
-
bioRxiv - Biochemistry 2023Quote: ... at 5% CO2 at 37 °C in accordance with standard mammalian tissue culture protocols and periodically tested for mycoplasma contamination via the MycoAlert Mycoplasma Detection kit (Lonza).
-
bioRxiv - Molecular Biology 2024Quote: ... 10 µg donor DNA and 10 µg Cas12a crRNA template DNA were diluted in either 20 µl (Lonza transfection) or 50 µl (Tb-BSF transfection ...
-
bioRxiv - Genomics 2023Quote: ... Neurons were nucleofected with 10 µ total DNA plasmid DNA using the AD1 4D-Nucleofector Y kit (Lonza V4YP-1A24) with the ED158 (iCell GlutaNeurons ...
-
bioRxiv - Cell Biology 2023Quote: ... 4 μM Alt-R Cas9 Electroporation enhancer (IDT) and 4 μM single-strand DNA homology template (Ultramer DNA Oligonucleotide, Lonza). After electroporation ...
-
bioRxiv - Microbiology 2024Quote: ... with the FlashGelTM DNA Marker Ladder (Lonza, 50473) for size confirmation ...
-
bioRxiv - Microbiology 2020Quote: ... DNA yield and quality was assessed by electrophoresis using a 0.75% agarose gel containing the DNA stain GelStar (Lonza, Rockland, ME, USA) in 1X Tris Borate EDTA (TBE ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA was imaged by staining with SYBR green (LONZA). All visual bands as well as the bands at the same locations in the other lanes were excised from the gel ...
-
bioRxiv - Cell Biology 2023Quote: ... Neurons were transfected with plasmid DNA using Nucleofector (Lonza) before plating as described (Minegishi et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... we used 2.2 % agarose gel electrophoresis (FlashGel DNA System, Lonza) and Bioanalyzer High-Sensitivity DNA Assay (Supplementary Figure 2) ...
-
bioRxiv - Systems Biology 2020Quote: ... Sheared DNA was run on a 1% agarose gel (Lonza) to verify that sheared DNA was between 100-1000 bp ...
-
bioRxiv - Immunology 2023Quote: The 3 kbp DNA plasmid encoding green fluorescent protein (GFP) (Lonza) was used to assess transfection efficiency ...
-
bioRxiv - Molecular Biology 2021Quote: ... phosphodiester linkages in the DNA backbone were cleaved by DNAse I (Lonza).
-
bioRxiv - Developmental Biology 2019Quote: ... The DNA constructs were introduced into the cells with Amaxa Nucleofector (Lonza).
-
bioRxiv - Molecular Biology 2024Quote: ... DNA samples were loaded on 3% NusieveTM GTGTM Agarose gel (Lonza, 50081), and electrophoresis was run at 100V in 1X TBE buffer for ∼50 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... Purified DNA was separated on a 3% NuSieve GTG agarose gel (Lonza #50081). The gel band corresponding to the size of dinucleosomal DNA (∼250 to 350 bp ...
-
bioRxiv - Genomics 2020Quote: ... Purified DNA was separated on a 3% NuSieve GTG agarose gel (Lonza #50081). The gel band corresponding to the size of dinucleosomal DNA (∼250 to 350bp ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with ~5 μg plasmid DNA using nucleofection (Amaxa, Lonza AG) according to manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2021Quote: DNA was delivered by nucleofection using the Nucleofector 2b system (Lonza VPH-1001). 1.5 million A17iCre mESCs were plated on gelatin-coated 10 cm dishes 2 days before delivery ...
-
bioRxiv - Genomics 2020Quote: ... The first consisted of 2 μl/well pmaxGFP DNA (LONZA,1 μg/ul) diluted in 250 μl/well Opti-MEM® medium (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: ... DNA from supernatants and pellets were analyzed on 1% agarose (Lonza cat. # 50000) gels in TAE buffer at 3 V/cm for 40 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... was resuspended in 110 µl P3+DNA solution (Lonza Cat. No. V4Xp-3024). Transfection of either free merozoites carried out in a 4D-Nucleofector System (Lonza) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cells were transfected with 2.26µg total plasmid DNA in SF buffer (Lonza, V4SC-2096), using program CM134 and incubated for 24 hours prior to luminescence measurement ...
-
bioRxiv - Biophysics 2022Quote: CH27 cells were transiently transfected with plasmid DNA encoding membrane anchor constructs by Lonza Nucleofector electroporation (Lonza ...
-
bioRxiv - Cell Biology 2022Quote: ... The cell/DNA mixture was then nucleofected using the 4-D nucleofector system (Lonza) code CB-150 and densely replated on Matrigel coated 6-well plates at 2.5×106 cells per well ...
-
bioRxiv - Microbiology 2021Quote: ... Parasites were transfected with 50-100 µg DNA using either the Amaxa system (Lonza) or the Biorad system as previously described (Moon et al. ...
-
bioRxiv - Cell Biology 2023Quote: ... and the 50 bp – 1.5 Kb FlashGel™ DNA Marker as ladder (Lonza, 57033). The 2100 Bioanalyzer of Automated Droplet Generator (Agilent ...
-
bioRxiv - Molecular Biology 2023Quote: ... All PCR products and double-stranded DNAs were purified with agarose gels (Lonza, 50004) and the GeneJet Gel Extraction Kit (Thermo Fisher ...
-
Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... 1 μg plasmid DNA was added and cells electroporated in a 4D-NucleofectorTM System (Lonza) following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2019Quote: ... plasmid DNA (50 µg per pool) was resuspended in 100 µl of buffer P3 (Lonza) with 4 µl ATP (625 mM) ...
-
bioRxiv - Molecular Biology 2020Quote: ... HeLa cells were transfected with the gRNAs and ss donor DNA using a nucleofector (Lonza, solution SE ...
-
bioRxiv - Microbiology 2024Quote: ... The DNA constructs in TE buffer were mixed with 90µl P3 Primary cell solution (Lonza) and used to resuspend 20µl segmented schizonts which were subsequently transferred to a transfection cuvette ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were electroporated with the corresponding DNA vector using a 4D-Nucleofector (Lonza, program DS-112) according to protocol of manufacturer and re-plated in plastic dish (Costar ...
-
bioRxiv - Microbiology 2020Quote: ... 25 μg linearized DNA were added to the parasites and then nucleofected using 4D-NucleofectorTM (Lonza) with setting EO-115 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Horizontal electrophoresis of DNA was carried out using 1% Seakem LE agarose gels (Lonza, Basel, Switzerland). All cloning and molecular biology experiments were carried out according to established protocols ...
-
bioRxiv - Biophysics 2022Quote: ... Jurkat cells were electroporated with 1μg of DNA plasmid pcDNA3.1_mLck_GFP (produced in the lab, AM Lellouch) with Nucleofector 2b device (Lonza), and selected by antibiotic G418 ...
-
bioRxiv - Neuroscience 2022Quote: ... neurons were transfected with a maximum of 0.6 μg total plasmid DNA using a Nucleofector (Lonza) and following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Dinucleosome-sized DNA fragments were purified and excised from a 3% NuSieve GTG agarose gel (Lonza) using the Zymoclean Gel DNA Recovery Kit (Zymo Research) ...