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Citations for Lonza :
401 - 450 of 3868 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Cells were transfected using the P3 Primary Cell 4D-Nucleofector™ X Kit S (Lonza-BioResearch, Cat #: V4XP-3032) and Amaxa™ 4D-Nucleofector™ (Lonza-BioResearch) ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were then mixed with 100uL of SE Cell Line 4D-Nucleofector® X Kit (Lonza, Basel, Switzerland) and transfected with 65nM siRNA diluted in MilliQ sterile-filtered water using the 4D-Nucleofector (Lonza ...
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bioRxiv - Immunology 2019Quote: ... Pre-activated B cells were transfected using the P4 Primary Cell 4D-Nucleofector X Kit L (Lonza, V4XP-4024) in combination with the program DI-100 ...
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bioRxiv - Immunology 2021Quote: ... The siRNA duplexes were used to transfect purified human naïve B cells using the Human B Cell NucleofectorTM Kit (VPA-1001, LONZA). Transfected B cells were then stimulated with CpG ODN 2395 plus IL-2 and IL-21 for 96 h before genomic DNA extraction for analysis of Sμ-σδand Sμ-Sγ1 DNA recombination ...
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bioRxiv - Biochemistry 2020Quote: ... Large-scale suspension cultures (300 mL) of High Five insect cells at 0.5 × 106 cells/ml were grown in Insect-Xpress media (Lonza) and inoculated with P2 baculovirus solution containing the Rpc5 constructs ...
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bioRxiv - Neuroscience 2021Quote: ... High Five cells were seeded 1 day prior to infection at 1×106 cells/mL in InsectXpress medium (Lonza). Cells were co-infected with low-passage recombinant baculovirus for COL6A3WT or COL6A3ΔC4 along with P4HA1/P4HB at a MOI of 2 and 0.2 respectively ...
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bioRxiv - Cell Biology 2021Quote: ... PBMCs were seeded onto collagen I-coated wells at a cell density of 1.5 - 2 × 106 cells/cm2 in endothelial growth media 2 (EGM-2) medium (Lonza) supplemented with 16% defined fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: R22 cells were transfected using the Amaxa™ 4D-Nucleofector™ Optimization Protocol for primary cells (Lonza, Morristown, NJ). R22 cells were grown in culture plates containing DMEM/High glucose supplemented with 10% FBS in a humidified 37’C/5% CO2 incubator ...
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bioRxiv - Immunology 2020Quote: Transfection of TnT cells and Jurkat-derived cell lines was performed by electroporation using the 4D-Nucleofector device (Lonza) and the SE cell line kit (Lonza ...
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bioRxiv - Cancer Biology 2020Quote: ... pCAGIG-hA3A-BE3 plasmid was delivered into cells using SF Cell Line 4D-Nucleofector X Kit (Lonza, #V4XC-2032) using programme CA-137 ...
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bioRxiv - Cancer Biology 2020Quote: ... primary glioma cells were isolated and red blood cells were lysed using ammonium-chloride-potassium (ACK) lysing buffer (Lonza). Tumor cells were resuspended in flow buffer (Hank’s Balanced Salt Solution with Ca2+ and Mg2+ ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were routinely tested for mycoplasma every 6 months to ensure cell quality (MycoAlert™ Mycoplasma Detection Kit; Lonza).
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bioRxiv - Cancer Biology 2022Quote: ... 5 μg of DNA was used to transfect the cells using the Amaxa Cell Line Nucleofector kit (Lonza Bioscience) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Two million cells were resuspended in 100 μl of P3 Primary Cell Nucle-ofector™ solution (V4XP-3024, Lonza). The RNP complex and Alt-R® Cas9 Electroporation Enhancer (1075916 ...
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Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiationbioRxiv - Developmental Biology 2022Quote: ... and re-seeded onto 0.2% gelatin-coated 10-cm dishes with 5.0 × 105 cells per dish in Endothelial Cell Growth Medium (EGM; Lonza) containing 20 ng/mL VEGF ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cells (1.6 × 106 cells* ml) were grown in 50 ml of Insect-Xpress medium (Lonza, Walkersville, MD, USA) with 15 mg/ml gentamycin (Roth ...
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bioRxiv - Immunology 2022Quote: ... primary human CD8+ T cells were thawed into complete human T cell media: X-VIVO 15 (Lonza # 04-418Q), 5% Human AB serum (Valley Biomedical #HP1022 ...
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bioRxiv - Microbiology 2021Quote: ... Pre-assembled Cas9-gRNA RNPs were electroporated into cells using Lonza Human T cell Nucleofector kit (Lonza, VPA-1002) and the Nucleofector2b equipment ...
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bioRxiv - Biochemistry 2021Quote: ... For each nucleofection 3 x 106 cells were resuspended in 80 μl of mouse ES cell nucleofection solution (Lonza). Equimolar (0.32 nmol ...
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bioRxiv - Cell Biology 2022Quote: ... the genetic transformation of RAW 264.7 cells was obtained by nucleofection using the Amaxa Cell Line Nucleofector Kit V (Lonza) for RAW 264.7 and following the protocol for Amaxa Nucleofector ...
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bioRxiv - Immunology 2022Quote: ... Five hundred thousand cells were pelleted and resuspended in RNP solution and 20ml of primary cell P3 buffer (Lonza), and were transferred to an electroporation curvette (Lonza) ...
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bioRxiv - Immunology 2022Quote: ... In vitro activated or naïve P14 cells were resuspended in 20 μl of P3 (P3 Primary Cell 4D-Nucleofector X Kit; Lonza), mixed with sgRNA/Cas9 RNP and electroporated using a Lonza 4D-Nucleofector system (CM137 ...
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bioRxiv - Immunology 2022Quote: RAW 264.7 macrophage-like cells were transiently transfected by nucleofection using Amaxa cell line kit V (Lonza; VACA-1003) as previously described115 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were transfected by nucleofection using the Lonza Cell Line Nucleofector® Kit V (Lonza, Basel, Switzerland, #VVCA-1003) and the Amaxa Nucleofector II device ...
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bioRxiv - Synthetic Biology 2019Quote: INS-1E cells were transfected with Cas9 RNP and ssODN using the SF Cell Line 4D-Nucleofector kit (Lonza) following the pulse program of DE-130 ...
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bioRxiv - Microbiology 2020Quote: A549 cells (human lung carcinoma cell line, ATCC CCL-185) were grown in Dulbecco’s modified eagle medium (DMEM) (Lonza) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... On day 7 cells were nucleofected using the Amaxa Mouse T Cell Nucleofector Kit (Lonza, Cat no. VVPA-1006) per the manufacturer’s instructions with 5x106 cells and 1.5µg plasmid DNA per cuvette ...
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bioRxiv - Genetics 2021Quote: Parallel nucleofections of Cas9 RNP and ssODN in KOLF2.1J cells were carried out in P3 Primary Cell buffer in 16-well cuvettes (Lonza) using an Amaxa 4D-Nucleofector (Lonza) ...
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bioRxiv - Biophysics 2021Quote: ... and resuspended at a density of ∼1.5x104 cells/μL in SF Cell Line 4D-Nucleofector Solution SF with supplement according to manufacturer instructions (Lonza). 2 μg of each Pcdh expression construct were transfected into 20 μL of the K562 cell suspension by electroporation using an Amaxa 4D- Nucleofector (Lonza) ...
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bioRxiv - Biophysics 2020Quote: SARS-CoV-2 was isolated from the oropharyngeal swab of a laboratory-confirmed COVID-19 patient in Hungary and passed two times in VeroE6 cell line (European Collection of Authenticated Cell Culture, Salisbury, U.K.) in Dulbecco’s Modified Eagle’s Medium (Lonza, Basel, Switzerland) supplemented with 5% fetal bovine serum (EuroClone ...
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bioRxiv - Genetics 2019Quote: PBT cells were transfected with 5 μg DNA for 5.106 cells in 100 μl of Human T Cell Nucleofector Solution (Lonza) with the program U-014 (Amaxa Biosystems).
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bioRxiv - Immunology 2021Quote: ... Cells were centrifuged for 5 min at 1200 rpm at 20°C and resuspended at 2.5×106 cells/ml in X-VIVO 15 cell culture medium (Lonza) with 50 U/ml penicillin–streptomycin ...
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bioRxiv - Immunology 2021Quote: Endothelial cells were transfected using the Nucleofector primary mammalian endothelial cell kit (Cat. #VAPI-1001, Lonza Biosciences, Durham, CA), according to manufacturer’s instructions ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... The resuspended cells were immediately transferred into the cuvette of the P3 Primary Cell Nucleofector Kit (Lonza; V4SP-3096) and electroporated using code EH-100 on the Lonza 4D-Nucleofector ...
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bioRxiv - Cell Biology 2022Quote: ... OCI-LY-1 cells were transfected using the Amaxa® Cell Line Nucleofector® Kit L (Lonza, Basel, Switzerland), program C-05 as previously described(46) ...
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bioRxiv - Immunology 2023Quote: ... Enriched naïve PbTII GFP+ cells were resuspended in 20μl of P3 Primary buffer (P3 Primary Cell 4D-Nucleofector X Kit, Lonza) and mixed with sgRNA/Cas9 RNP ...
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bioRxiv - Cancer Biology 2023Quote: ... 3uL of the total Cas9-RNP complex was added to 200,000 K562 cells resuspended in 18uL SF cell solution (Lonza) and subjected to nucleofection with the Lonza 4D nucleofectorsystem using manufactured specified conditions for K562 cells ...
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bioRxiv - Bioengineering 2023Quote: ... and 40 pmol of single-stranded oligonucleotide donor DNA (ssODN) were nucleofected to 0.25 × 106 HEK293T cells using the SF Cell Line 4D-Nucleofector kit (Lonza) following the pulse program of CM-130 ...
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bioRxiv - Cell Biology 2023Quote: ... and mN designates the 2’-O-methylribonucleotides) was transfected into HAP1 cells (1.0 × 106 cells) by Nucleofector Kit V (Lonza) with a Nucleofector device (Lonza ...
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bioRxiv - Immunology 2023Quote: ... and red blood cells co-sedimented with the intrahepatic immune cells (IHICs) were lysed using ACK lysis buffer (Lonza) for 5 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were pulsed with protocol EO115 using the P3 Primary Cell 4D-Nucleofector Kit and 4D-Nucleofector System (Lonza). Cells were recovered immediately with warm media for 6 hours before transduction with CAR ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T cells (1.7 x 105 cells/well in 12-well plates) were grown for 24 h in supplemented DMEM (Lonza), before to be incubated with 20 μg/mL 55Fe-transferrin for 24 h and transiently transfected with wild-type or mutated FPN1-V5 plasmid constructs ...
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bioRxiv - Bioengineering 2024Quote: ... and SK-BR-3 cells (Korean Cell Line Bank, Korea) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Lonza, Switzerland) supplemented with 10 % (v/v ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were targeted by nucleofection using the SE Cell Line 4D-Nucleofector™ X Kit S (Lonza #V4XC-1032). Cells were allowed to recover from nucleofection before sib-selection-based subcloning to isolate pure clonal cultures ...
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bioRxiv - Synthetic Biology 2024Quote: ... Isolated T cells were cultured at an initial density of 106 cells/mL in X-Vivo 15 medium (Lonza) supplemented with human serum (5% ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293T cells (1.7 × 105 cells/well in 12-well plates) were grown for 24 h in supplemented DMEM (Lonza), before to be incubated with 20 µg/mL 55Fe-transferrin for 24 h and transiently transfected with wild-type or mutated FPN1-V5 plasmid constructs ...
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bioRxiv - Molecular Biology 2024Quote: ... 400 pmol of siRNA duplex was introduced into 3.0 × 106 cells using the Cell Line 96-well Nucleofector Kit SF (Lonza) with program DG150 of the 96-well Shuttle Device (Lonza) ...
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bioRxiv - Cancer Biology 2024Quote: ... and (iv) the NDF-2 and NDF-3 cell lines (human neonatal dermal fibroblast cell lines, #CC-2509; Lonza Bioscience ...
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bioRxiv - Microbiology 2020Quote: ... Infected CD4 T cells were washed with PBS and 3 million cells per condition were resuspended in 20uL buffer P2 (Lonza) with 4μM IDT electroporation enhancer ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 500,000 cells were transfected using the using the mouse neural stem cell nucleofection kit (Lonza, VPG-1004) with 0.66 μg of the construct and 0.33 μg of transposase using the Lonza Amaxa Nucleofector I with protocol A-33 ...