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Citations for Lonza :
151 - 200 of 1192 citations for 7 Fluoro 6 amino 2H 1 4 benzoxazin 3 4H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: K562 cells were transfected with two gRNAs targeting exon 1 and 3 of NGLY1 and Cas9 plasmid (lentiCas9-Blast) by Nucleofection according to the manufacturer’s protocol (Nucleofector, Lonza). LentiGuide-Puro (Addgene plasmid #52963 ...
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bioRxiv - Immunology 2022Quote: ... -coated 6-well plates in X-VIVO 15 media (Lonza), supplemented with rhActivin A ...
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bioRxiv - Biochemistry 2020Quote: ... Tissues were embedded in 6% low-melting point agarose (Lonza) for two minutes on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5% (V/V) FCS (LO CC-3202/6, Lonza) up to 70-75% confluency ...
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bioRxiv - Immunology 2024Quote: ... and used at passage 6 (Lonza, CC-3156 & CC-4147). Primary human brain pericytes were obtained from Cell Systems (ACBRI 498) ...
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bioRxiv - Bioengineering 2020Quote: ... For transfection 4 × 106 cells/mL cells were resuspended in ProCHO-4 Medium (Lonza) supplemented with 4 mM Ultraglutamine (Lonza) ...
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bioRxiv - Genomics 2022Quote: ... donors (passage 4-9; Lonza) were grown in complete Endopan-3 medium kit (PAN-Biotech ...
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bioRxiv - Cell Biology 2023Quote: ... or 4-D nucleofector (LONZA) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... One million cells were electroporated using the Amaxa 4D Nucleofector kit (V4XC-9064, Lonza). Guide RNA and Cas9 complexes were pre-formed using 160μM crRNA annealed to 160μM tracrRNA (Dharmacon ...
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bioRxiv - Cell Biology 2020Quote: ... and 7 mM Na2HPO4 in nuclease-free water) using either the Amaxa nucleofector system II (Lonza) under nucleofection program T-005 or the Amaxa 4Dnucleofector system (Lonza ...
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bioRxiv - Neuroscience 2021Quote: ... 7 µg mRNA was transfected into 0.5× 106 fibroblasts using Cell Line Nucleofector Kit V (Lonza) and AMAXA program X-001 following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Measured tissues originated from six F508del homozygous patients (CF patient #1: CF-AB0609, female, 31 years-old; CF patient# 3: #28388-0000450918, Lonza, male ...
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bioRxiv - Cell Biology 2024Quote: ... together with a plasmid encoding the piggyBac transposase in a 3:1 ratio (vector:transposase) using the P3 Primary cell 4D-Nucleofector™ kit (Lonza). Prior to targeting ...
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bioRxiv - Bioengineering 2022Quote: ... and 3% FBS (all from Lonza Biosciences), termed astrocyte medium ...
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bioRxiv - Microbiology 2020Quote: ... or 6 days in growth factor-free Keratinocyte Basal Medium (Lonza) supplemented to achieve 1.5 mM [final] CaCl2.
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bioRxiv - Neuroscience 2021Quote: ... 6 hours post transfection cells were switched to Ultraculture medium (Lonza) and grown for another 24-48hrs ...
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bioRxiv - Immunology 2023Quote: ... and embedded in 6% w/v low melting point agarose (Lonza) in 1x PBS ...
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bioRxiv - Bioengineering 2021Quote: ... were used between passages 3-5 for paxillin experiments and culture medium was changed every 2-3 days (Lonza FBM Basal Medium supplemented with 2 v/v% fetal bovine serum (FBS) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Dry powder (2-3 mg) was loaded into an HPMC size 3 capsule (VCaps® Plus, Lonza, Morristown, NJ). The capsule was placed in a Plastiape high resistance RS00 DPI that was then attached to a Next Generation Impactor (NGI ...
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bioRxiv - Developmental Biology 2023Quote: ... were cultured in mEC medium (0.1% bovine brain extract (BBE)/10% FBS/1%penicillin-streptomycin/1% Non-Essential Amino Acid (NEAA)/Dulbecco Modified Eagle Medium (DMEM) (DMEM Cat#: 41966-029, Gibco; BBE Cat#: CC-4098, Lonza BioScience). All cells were cultured at 37 °C in a humidified atmosphere with 5% CO2 (HDLECs and HUVECs ...
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bioRxiv - Bioengineering 2019Quote: ... The plate containing the amplified genes or qPCR products was kept in ice and observed in a 2% agarose gel (NuSieve® 3:1 Agarose (Lonza)) to check and reinforce the identity of the amplicons ...
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bioRxiv - Microbiology 2021Quote: ... Vero cell cultures were seeded at 1-3 × 104 cells/cm2 in Dulbecco’s minimal essential medium (DMEM, LONZA, Alpharetta, GA, USA) supplemented with 9% foetal bovine serum (FBS ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and PX458-AAVS1 (3 µg) were electroporated into 1.3×106 H9 cells using the Human Stem Cell Nucleofector Kit 1 (Lonza, VPH-5012). Following electroporation the cells were seeded across 3-wells of a 6 well plate coated with Matrigel in StemFlex supplemented with RevitaCell supplement (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... supplemented with 4 mM Ultraglutamine (Lonza). 0.625 μg of plasmid DNAs per million cells followed by 2.5 μg polyethyleneimine per million cells (Polysciences ...
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bioRxiv - Immunology 2021Quote: ... suspension CHO cells expressing wildtype human CTLA-4 were plated at 1 × 106 cells/well in DPBS (Lonza, Basel, Switzerland) containing 0.5% BSA (MilliporeSigma ...
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bioRxiv - Cancer Biology 2019Quote: 1-4 × 106 dissociated cells were re-suspended in 100 µL of Amaxa Mouse NSC Nucleofector Solution (VPG-1004, Lonza) with 5 µg of plasmid DNA ...
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bioRxiv - Microbiology 2020Quote: ... The same number of cells (2 x 106 cells) were lysed in 100 µl of 1% CHAPS/PBS and analyzed on 4-20% SDS-PAGE gel (Lonza). The envelope proteins were detected by 2F5 (specific for gp41 ...
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bioRxiv - Immunology 2022Quote: ... supplemented with fetal calf serum (FCS, 3%, Lonza) and HEPES (1.8%) ...
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bioRxiv - Immunology 2022Quote: ... supplemented with fetal calf serum (FCS, 3%, Lonza) and HEPES (1.8% ...
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bioRxiv - Cell Biology 2019Quote: ... and separated on a 3% MetaPhor gel (Lonza).
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bioRxiv - Microbiology 2023Quote: ... and 3 ml of ACK lysis buffer (Lonza) was added to the cells and incubated for 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... NHEM-Ad cells were grown in MGM-4 Melanocyte Growth Medium-4 BulletKit (Lonza, Cat# CC-3249). We added penicillin (50 U/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... and 199 (M199) medium (3: 1) supplemented with 10% fetal bovine serum (FBS),10 mM glutamine and penicillin-streptomycin (all from Lonza, Basel, Switzerland) and their purity was verified with the fibroblast marker TE-7 (Merck ...
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bioRxiv - Cancer Biology 2023Quote: MCF-7 and MDA-MB-231 (ECACC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Lonza, Manchester, UK), supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2019Quote: ... NHAs were used within one passage and maintained in astrocyte growth medium (AGM, Lonza #CC-3187) supplemented with L-glutamine ...
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bioRxiv - Cancer Biology 2019Quote: ... were transfected using 4-D electroporator (LONZA), following manufacturer instruction ...
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bioRxiv - Immunology 2022Quote: ... and 4 mM of L-glutamine (Lonza). HLA-E expressing K562 cells were sorted and maintained in complete RPMI supplemented with 2 µg/mL blasticidin (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... Human umbilical vein endothelial cells (HUVECs) (up to passage 6; Lonza, Wokingham, UK) were cultured in EGM-2 medium (Lonza ...
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bioRxiv - Paleontology 2019Quote: ... and rib bones of a 6-year-old female (NHOst-Osteoblasts by Lonza Pharma ...
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bioRxiv - Cell Biology 2019Quote: ... and routinely (every 6 months) tested negative to mycoplasma (MycoAlert, LT07-218, Lonza).
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bioRxiv - Plant Biology 2023Quote: ... They were then embedded in 6% SeaKem® LE agarose (Lonza, Basel, Switzerland), and sections of 100 µm were realized using a vibrating blade microtome (Microm 650V ...
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bioRxiv - Biophysics 2021Quote: ... The WT and mutant genes were then amplified from these expression plasmids using the PCR primers 5’-GCGCTAGCAGCACCCATATGCCTGAG-3’and 5’-ACGAAGCTTTCAGTGGTGGTGGTGGT-3’and subcloned into the pMaxCloning vector (Lonza, VDC-1040) via NheI and BamHI sites to generate the pMax_WT_NEIL1 and pMax_Mutant_NEIL1 expression plasmids that were utilized throughout this analysis.
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bioRxiv - Immunology 2021Quote: ... On day 7 cells were nucleofected using the Amaxa Mouse T Cell Nucleofector Kit (Lonza, Cat no. VVPA-1006) per the manufacturer’s instructions with 5x106 cells and 1.5µg plasmid DNA per cuvette ...
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bioRxiv - Bioengineering 2023Quote: ... rinsed 3 times with 1x Dulbecco’s phosphate buffered saline (DPBS) (Formulation: 200mg/L KCl and KH2PO4, 8000mg/L NaCl, 2160mg/L Na2HPO4*7H2O, pH 7-7.6,) (BioWhittaker, Lonza) fixed with 3.7% paraformaldehyde solution prior to running in flow cytometer (Guava easyCyte 6HT ...
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bioRxiv - Genetics 2023Quote: ... IMR90 fetal lung fibroblasts at passage 7 were cultured in FGM-2 lung fibroblast basal media (Lonza, #CC-3131) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... embedded in 3% agarose (Lonza, # 50004, Rockland, ME, USA) and coronally sectioned (Leica VT1000S vibratome ...
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bioRxiv - Genomics 2022Quote: ... One million sorted Pro-E cells were resuspended with 100 μl solution P3 for primary cells (Lonza); preassembled RNP were then added to the cell suspension for nucleofection ...
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bioRxiv - Molecular Biology 2023Quote: One million Aire wild-type mTEC 3.10 cells were suspended in 100 μL of nucleofector solution (Lonza) containing 20 pmol of spCas9-GFP ...
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bioRxiv - Bioengineering 2020Quote: ... About 2–4 passages of primary keratinocytes were made in culture in 37°C in a 7 % CO2 incubator with KBM-Gold supplemented with KGM-Gold Single Quots (Lonza) medium to 80% confluence in 100 mm Petri dishes ...
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bioRxiv - Microbiology 2021Quote: ... Raw264.7 Ifit2 CRISPR cells were generated by electroporating low passage Raw264.7 cells with Ifit2 pSBtet-puro-Cas9-U6 using Amaxa Nucleofector II and Amaxa Cell Line Nucleofector Kit V (Lonza). Cells were selected with puromycin 3 days post-nucleofection ...