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Citations for Lonza :
51 - 100 of 104 citations for 7 bromomethyl benzo b phenanthrene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... and 2.5 μg/mL amphotericin B in Hank’s balanced salt solution (HBSS) (Lonza, UK). It was then finely minced and digested overnight on a rocker at 4 °C in 1 mg/mL protease XIV (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... were expanded to passage 6 or 7 in endothelial growth media (EGM-2 BulletKit, Lonza), and culture medium was changed every other day ...
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bioRxiv - Immunology 2023Quote: ... and cells were harvested on day 7 using 1 X PBS EDTA (Lonza, BE02-017F) for experiments.
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bioRxiv - Immunology 2019Quote: ... B cells were cultured for 2 to 4 days in RPMI 1640 with UltraGlutamine (Lonza) containing 10% fetal calf serum (FCS ...
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bioRxiv - Cancer Biology 2021Quote: ... Normal human bronchial epithelial (NHBE)-Bronchial Epi Cells for B-ALI from Lonza (CC-2540S) were cultured in PneumaCult-Ex Plus Medium (StemCell ...
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bioRxiv - Bioengineering 2020Quote: Bone marrow derived human mesenchymal stem cells (BM-hMSCs) (24yr old B female, Lonza, Switzerland) were used at passage 4 – 6 and cultured with complete hMSC media containing low glucose Dulbecco’s Modified Eagle Medium and glutamine (School of Chemical Sciences Cell Media Facility ...
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bioRxiv - Immunology 2022Quote: ... Purified B cells were then cultured at 0.5 ×106 cells/mL in RPMI 1640 (Lonza) containing penicillin (100 IU/ml) ...
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bioRxiv - Cell Biology 2020Quote: ... and 7 mM Na2HPO4 in nuclease-free water) using either the Amaxa nucleofector system II (Lonza) under nucleofection program T-005 or the Amaxa 4Dnucleofector system (Lonza ...
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bioRxiv - Neuroscience 2021Quote: ... 7 µg mRNA was transfected into 0.5× 106 fibroblasts using Cell Line Nucleofector Kit V (Lonza) and AMAXA program X-001 following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... B cells were cultured in X-VIVO™15 media (04-744Q, Lonza, Walkersville, MD, USA) with penicillin-streptomycin (100 units/mL ...
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bioRxiv - Cancer Biology 2022Quote: Nucleofection experiments of ALL-B cells were carried out using Amaxa 4D-Nucleofector X kit L (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Bone marrow derived DCs at day 7 (3×106) were transfected with 100μl of the Amaxa solution (Lonza) containing siRNA (control or target-specific ...
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bioRxiv - Cancer Biology 2023Quote: MCF-7 and MDA-MB-231 (ECACC) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Lonza, Manchester, UK), supplemented with 10% (v/v ...
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bioRxiv - Immunology 2022Quote: ... 2×106 B cells were then nucleofected with 2 μg of plasmid DNA using Nucleofector Kit V (Lonza) and rested for at least 16-24 hours using complete media containing 5 ng/ml BAFF and lacking LPS ...
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bioRxiv - Microbiology 2019Quote: ... and 25 ng/ml of amphotericin B. HEK293 cells (obtained from M. Marketon’s laboratory) were maintained in Dulbecco’s modified essential medium (DMEM) (Lonza) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... dPPM were stored at 4°C in PBS containing 1X penicillin/streptomycin/amphotericin B solution (100X PSA containing 10,000 units potassium penicillin/mL, 10,000 µg streptomycin sulphate/mL and 25µg amphotericin B/ml in 0.85% saline, Lonza, Switzerland) for two weeks before being transferred to −80°C ...
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bioRxiv - Immunology 2021Quote: ... On day 7 cells were nucleofected using the Amaxa Mouse T Cell Nucleofector Kit (Lonza, Cat no. VVPA-1006) per the manufacturer’s instructions with 5x106 cells and 1.5µg plasmid DNA per cuvette ...
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bioRxiv - Bioengineering 2023Quote: ... rinsed 3 times with 1x Dulbecco’s phosphate buffered saline (DPBS) (Formulation: 200mg/L KCl and KH2PO4, 8000mg/L NaCl, 2160mg/L Na2HPO4*7H2O, pH 7-7.6,) (BioWhittaker, Lonza) fixed with 3.7% paraformaldehyde solution prior to running in flow cytometer (Guava easyCyte 6HT ...
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bioRxiv - Genetics 2023Quote: ... IMR90 fetal lung fibroblasts at passage 7 were cultured in FGM-2 lung fibroblast basal media (Lonza, #CC-3131) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... Pre-activated B cells were transfected using the P4 Primary Cell 4D-Nucleofector X Kit L (Lonza, V4XP-4024) in combination with the program DI-100 ...
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bioRxiv - Microbiology 2022Quote: ... NHBE cells were infected with viruses at an MOI of 0.01 and cultured in B-ALI growth medium (Lonza) at 33°C ...
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bioRxiv - Developmental Biology 2019Quote: ... were nucleofected into 1×106 pluripotent RUES2 cells using the B-016 setting on an Amaxa Nucleofector II (Lonza). Nucleofected cells were then plated as per maintenance conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were nucleofected with 10ug lineage recorder DNA and 1ug Sleeping Beauty transposase following the manufacturer’s protocol and using the B-16 program of the Nucleofector 2b (Lonza) in cuvettes for 100μl Human Stem Cell nucleofection buffer (Lonza ...
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bioRxiv - Cell Biology 2021Quote: ... were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% (v/v) fetal bovine serum (FBS) and 1X penicillin/streptomycin/amphotericin B (Lonza) at 37°C with 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: ... 0.1% r-human fibroblast growth factor (rhFGF) and 0.1% Gentamicin sulfate amphotericin-B (GA-1000) (Lonza, Quakertown, PA, USA). HLFs were used from passages 1 to 10 ...
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bioRxiv - Biochemistry 2019Quote: ... at passages 4-7 were grown to confluence on gelatin-coated plates and maintained using complete EGM-2 media (Lonza). HKi002 was maintained at room temperature for 30 min rotating before use ...
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bioRxiv - Bioengineering 2020Quote: ... About 2–4 passages of primary keratinocytes were made in culture in 37°C in a 7 % CO2 incubator with KBM-Gold supplemented with KGM-Gold Single Quots (Lonza) medium to 80% confluence in 100 mm Petri dishes ...
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bioRxiv - Microbiology 2021Quote: ... Raw264.7 Ifit2 CRISPR cells were generated by electroporating low passage Raw264.7 cells with Ifit2 pSBtet-puro-Cas9-U6 using Amaxa Nucleofector II and Amaxa Cell Line Nucleofector Kit V (Lonza). Cells were selected with puromycin 3 days post-nucleofection ...
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bioRxiv - Microbiology 2020Quote: ... fumigatus collected from three 10-cm plates grown for 7 days at 37°C were germinated with shaking in RPMI 1640 (Lonza) liquid media supplemented with glucose to a final concentration of 2% (w/v ...
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bioRxiv - Developmental Biology 2022Quote: Primary HDLECs were isolated from neonatal human foreskins as described previously (74) and cultured from passages 5 – 7 on fibronectin-coated plates with EGM-2 MV growth media (Lonza) supplemented with human VEGF-C (R&D) ...
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bioRxiv - Molecular Biology 2023Quote: ... the paired RNPs were combined and nucleofected into 4×105 Raw 264-7 cells suspended in 10 ul of nucleofection buffer (Lonza) using the program DS-136 in a 4D Nucleofector X Unit (Lonza) ...
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bioRxiv - Cancer Biology 2019Quote: ... Human cerebral microvascular endothelial cells (hCMEC/D3 [hCMEC]; gift from Dr. B. Weksler, Cornell Medical College) were grown in endothelial basal medium-2 (Lonza) supplemented with 5% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of eGFP-Centrin1 was achieved by electroporating 4.106 B lymphoma cells with 4 μg of plasmid using the Amaxa Cell Line Nucleofector Kit R (T-016 program, Lonza). Cells were cultured in CLICK medium for 5 to 16 h before imaging.
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bioRxiv - Neuroscience 2022Quote: ... and 252 pmol Cas9-HiFi or Cpf1-Ultra (both IDT) using the B-16 program of the Nucleofector 2b Device (Lonza) in cuvettes for 100 µl Human Stem Cell nucleofection buffer (Lonza ...
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bioRxiv - Immunology 2022Quote: ... Knockdown of Vps4a/b was performed using Amaxa Nucleofector II and the Mouse T-Cell Nucleofector Kit (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... A20 and A20 D1.3 B cells (3 × 106cells) were transiently transfected using the AMAXA nucleofector kit V (Lonza, #VCA-1003) or the Ingenio electroporation kit (Mirus ...
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bioRxiv - Immunology 2021Quote: Spleens and cervical lymph nodes were isolated from 6-10 weeks old C57B6 mice and homogenized through a sieve in B cell culture medium (RPMI-1640 (Lonza), 10% fetal calf serum (FCS ...
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bioRxiv - Immunology 2022Quote: ... was prepared by mixing 250 nmol target gene sgRNA+ 50 nmol eGFP sgRNA+ 50 nmol SpCas9 2NLS Nuclease (Synthego) in 50 μL mouse B cell nucleofection buffer (Lonza) at RT for 30min ...
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bioRxiv - Cancer Biology 2023Quote: ... containing a combination of the px458 plasmid targeting each gene and the ssODN and then electroporated using B-016 program of Nucleofector 2b (Lonza). The electroporated GSCs were cultured for 48 h followed by Single cell sorting of GFP-positive cells (SH800 ...
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bioRxiv - Bioengineering 2022Quote: ... B and T cell electroporations were performed using the Amaxa™ 96-well Shuttle™ with the 4D Nucleofector (Lonza). HSPC electroporations were performed in MaxCyte ATx ...
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bioRxiv - Biochemistry 2022Quote: ... ALI culture conditions were initiated when cells reached confluence by removal of apical medium and replacement of basolateral growth medium with B-ALI differentiation medium (cat# 193517, Lonza) supplemented with SingleQuots (cat# 193515 ...
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bioRxiv - Neuroscience 2023Quote: ... we used the human iPSC line SFC840-03-01 (STBCi026-B) generated within the StemBANCC project (part of Innovative Medicines Initiative) based on a commercially available fibroblast line (Lonza) from a healthy 67-years old female donor (Morrison et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Differentiation medium consisted of 50 % DMEM with pyruvate and 50 % BEBM supplemented with BEGM singlequots (except amphotericin B, triiodothyronine, and retinoic acid) (Lonza). Medium was supplemented with 100 nM all-trans retinoic acid (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... NHDF cells were cultured in FBM fibroblast basal media (supplemented with FGM-2 SingleQuot supplements: insulin, hEGF-B, GA-1000, and FBS) (Lonza). NHEK cells were expanded in KBM gold basal medium (KGM gold supplemented with Lonza’s SingleQuot supplements ...
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bioRxiv - Cell Biology 2021Quote: ... the media was changed to α-MEM containing macrophage colony-stimulating factor (M-CSF; 10 ng/ml) and and 66ng/ml receptor activator of nuclear factor kappa-B ligand (RANKL, Lonza Biosciences). OC activity was measured by releasing the europium conjugated human Type I collagen-coated on the bottom of the OsteoLyse™ Assay Kit (Lonza Biosciences ...
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bioRxiv - Immunology 2021Quote: ... The purified CD19+ B cells were cultured in the chemical-defined and serum-free X’VIVO 15 medium (Lonza, Walkersville, MD, USA), in the absence or presence of 100 U/ml penicillin ...
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bioRxiv - Systems Biology 2024Quote: ... MT2 and MDA-MB-231 cells with HLA-A,B,C CRISPR-Cas9 knockout were generated through electroporation of Cas9-sgRNA ribonucleoprotein (RNP) complex using 4D-Nucleofector (Lonza Bioscience). Three sgRNAs targeting HLA gene (HLA sgRNA#1 ...
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bioRxiv - Biophysics 2019Quote: Zebrafish between 9 and 13 dpf were anesthetized with Tricaine-S (MS-222, Pentaire; .008% w/v, buffered to pH 7) and embedded in 2% low melting point agarose (Lonza SeaPlaque, #50100) with .004% w/v Tricaine ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultivated together with mixed glial cells plated on the bottom of 12-well plates for 7 days in EBM-2 medium (Lonza, Basel, Switzerland) containing 15% PDS ...
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bioRxiv - Synthetic Biology 2024Quote: Electroporation was done 7 days after stimulation by DynaBeads using a P3 Primary Cell 4D-Nucleofector™ X Kit (Lonza #V4XP-3012). 750 ng of HDR template was mixed with 50 pmol of RNP and incubated at room temperature for 10 minutes ...