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Citations for Lonza :
1 - 50 of 2283 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Between 1×10^3 – 5×10^4 cells were re-suspended in 20μl Buffer P3 (Lonza) per reaction and quickly added to the Eppendorf tube containing the CRISPR/Cas9 gRNA RNP complex ...
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bioRxiv - Molecular Biology 2021Quote: Inguinal white adipose tissue from 3-4 WT-C57Bl/6 male mice was collected and placed in Dulbecco’s modified eagle’s medium (DMEM; Lonza) supplemented with 1% Penicillin/Streptomycin (PS ...
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bioRxiv - Cell Biology 2022Quote: ... followed by 3-4 days of culture in SmGM complete media (Lonza) supplemented with 1% penicillin-streptomycin to expand cell numbers ...
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bioRxiv - Cancer Biology 2023Quote: ... Every 3-4 weeks the medium was additionally supplemented with MycoZap (Lonza) to prevent mycoplasma contamination ...
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bioRxiv - Neuroscience 2023Quote: ... split into 3-4 wells each and cultured in DMEM-F12 (Lonza) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... were isolated from healthy donors (n=3) as described[4] and seeded on 6 cm dishes within BEGM media (Lonza) before transduction with lentivirus (empty vector (Origene ...
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bioRxiv - Biochemistry 2020Quote: One straw of passage 4 HUVECs (Lonza, USA) was thawed in a 35mm dish and cultured in EGM-2 medium until almost 80% confluence is reached ...
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bioRxiv - Neuroscience 2023Quote: ... iPS cells were passaged every 3-4 days with Accutase (Lonza, Basel, Switzerland) and seeded on new plates with density 1:5 – 1:10 in iPS medium with ROCK inhibitor Y-27632 (Sigma-Aldrich) ...
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bioRxiv - Genetics 2019Quote: ... Between 1×10^4 – 5×10^4 cells per condition were resuspended in 20μl of Buffer P3 (Lonza) per reaction and quickly added to the Eppendorf tube containing the Cas9 gRNA RNP complex ...
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bioRxiv - Immunology 2019Quote: ... B cells were cultured for 2 to 4 days in RPMI 1640 with UltraGlutamine (Lonza) containing 10% fetal calf serum (FCS ...
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bioRxiv - Genetics 2020Quote: ... ∼1×106 cells were transfected with pairs of 5 µg gRNA plasmid and 4 µg ssODN (Supplementary Table 3) using AmaxaTM Human CD34 Cell NucleofectorTM Kit (Lonza) in the 2B-NucleofectorTM on the U-08 setting ...
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Synthetic lethal targeting of TET2-mutant hematopoietic stem and progenitor cells by XPO1 inhibitorsbioRxiv - Cancer Biology 2022Quote: ... or either of two different gRNAs targeting exon 3 coding sequences of human TET2 (TET2-gRNA #1: 5’-TGGAGAAAGACGTAACTTC-3’ and TET2-gRNA #2: 5’-TCTGCCCTGAGGTATGCGAT-3’) were transfected with Nucleofector (Lonza). After transduction ...
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bioRxiv - Neuroscience 2021Quote: ... Tissues were washed 3-4 times over 1 hour in PBS (calcium- and magnesium-free; Lonza BioWhittaker #17-517Q) containing 0.1% Triton X-100 (PBT ...
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bioRxiv - Neuroscience 2019Quote: ... After 3 to 4 passages cell were electroporated via Nucleofection with the AMAXA nucleofection device (LONZA). The Neurospheres were dissociated with Accutase (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were passaged 1:10 every 3-4 days by trypsinisation and were regularly screened for mycoplasma contamination (Mycoalert, Lonza).
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bioRxiv - Microbiology 2024Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) 25 mM (Lonza, 17-737F), gentamicin 25 μg/ml (Lonza ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... supplemented with Ultraglutamine-1 (4 mM; Lonza) and antibiotic-antimycotic (1% ...
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bioRxiv - Systems Biology 2021Quote: ... and routinely passaged every 4∼6 days with Versene (EDTA) solution (Lonza). Selected iPSC clones were further validated for expression of pluripotency markers with immunostaining (Supplementary Fig ...
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bioRxiv - Systems Biology 2021Quote: ... and routinely passaged every 4~6 days with Versene (EDTA) solution (Lonza). Selected iPSC clones were further validated for expression of pluripotency markers with immunostaining (Chen et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Regular testing for mycoplasma contamination was conducted every 3-4 weeks using the MycoAlert PLUS mycoplasma detection kit (Lonza).
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bioRxiv - Immunology 2020Quote: ... Sorted B cells were cultured for 4 days in RPMI 1640 with UltraGlutamine (Lonza) containing 10% FBS (Dominique Dutscher) ...
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bioRxiv - Molecular Biology 2020Quote: ... at Day 3-4 of phase I culture were electroporated using P3 Primary Cell 4D NucleofectorTM X Kit (from Lonza) with program DZ100 (Bak et al. ...
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bioRxiv - Immunology 2021Quote: ... 2.106 T cells were resuspended in 20 µl of nucleofection solution with 3 µl or 4 µl RNP and transferred to Nucleofection cuvette strips (4D-Nucleofector X kit S; Lonza). Murine T cells were electroporated using the DN110 program of 4D nucleofector (4D-Nucleofector Core Unit ...
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bioRxiv - Bioengineering 2021Quote: ... HUVECs (Lonza, Passage <4) were cultured in an endothelial growth medium (C-22111 ...
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bioRxiv - Microbiology 2022Quote: ... for 24 h then interleukin-2 (50U/ml) for 4-6 days before nucleofection (as recommended by the manufacturer, Lonza) or infection by HIV-1.
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bioRxiv - Bioengineering 2021Quote: ... were used between passages 3-5 for paxillin experiments and culture medium was changed every 2-3 days (Lonza FBM Basal Medium supplemented with 2 v/v% fetal bovine serum (FBS) ...
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bioRxiv - Bioengineering 2020Quote: ... passage 3-5 AFC were dissociated and resuspended in EGM-2 (Lonza) at 4×105 cells/mL ...
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bioRxiv - Cell Biology 2021Quote: ... Human primary hepatic stellate cells from donors 3 and 4 were purchased as isolated hepatic stellate cells from Lonza (cat# HUCLS). Donor information is listed below.
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bioRxiv - Genomics 2020Quote: ... donors (passage 2-3; Lonza) were continuously passaged to replicative exhaustion in complete Endopan-2 supplemented with 2% FBS under 5% CO2 ...
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bioRxiv - Bioengineering 2020Quote: ... For transfection 4 × 106 cells/mL cells were resuspended in ProCHO-4 Medium (Lonza) supplemented with 4 mM Ultraglutamine (Lonza) ...
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bioRxiv - Genomics 2022Quote: ... donors (passage 4-9; Lonza) were grown in complete Endopan-3 medium kit (PAN-Biotech ...
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bioRxiv - Cell Biology 2023Quote: ... or 4-D nucleofector (LONZA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Transfection of primary T cells with Cas9 RNP complexes and TCRβα HDR templates was performed 3-4 days following activation using the 4D-Nucleofector and a 20 uL format P3 Primary Cell kit (Lonza, V4XP-3032). Briefly ...
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bioRxiv - Immunology 2021Quote: Commercially available human primary PTs from 6 donors (3 males and 3 females, Lonza Walkersville Inc) were expanded at passage 4 ...
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bioRxiv - Molecular Biology 2022Quote: ... separated on a 2% or 4% agarose gel (Seakem LE agarose, Lonza #50004) by electrophoresis (120 V ...
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bioRxiv - Molecular Biology 2023Quote: ... HPAECs were cultured for 4 hours in endothelial basal medium (EBM-2, Lonza) with 2% FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... 2-4 x 105 cells were resuspended in 100uL of P2 medium (Lonza) containing 10 µg of Cas9 or Cas9/guide plasmidic DNA and transferred to a cuvette (Lonza) ...
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bioRxiv - Bioengineering 2020Quote: ... supplemented with 4 mM Ultraglutamine (Lonza). 0.625 μg of plasmid DNAs per million cells followed by 2.5 μg polyethyleneimine per million cells (Polysciences ...
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bioRxiv - Microbiology 2023Quote: ... with a final concentration of 2% formaldehyde and 2% NuSieve 3:1 agarose (Lonza). 5 µg RNA was denatured for 10 min at 70 °C with 1 X MOPS buffer (20 mM MOPS ...
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bioRxiv - Genomics 2020Quote: ... Human umbilical vein endothelial cells were isolated from umbilical cords of healthy donors after cesarean sections (HUVECS; n = 4; RWTH Aachen) (55) or obtained from Lonza (n = 3, Basel, Switzerland) (8) ...
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bioRxiv - Cancer Biology 2022Quote: ... NHEM-Ad cells were grown in MGM-4 Melanocyte Growth Medium-4 BulletKit (Lonza, Cat# CC-3249). We added penicillin (50 U/mL ...
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bioRxiv - Immunology 2022Quote: ... for 4 days with complete EGM-2 media (Lonza CC-4147, Lonza CC-3156). Upon reaching 80-90% confluence ...
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bioRxiv - Immunology 2022Quote: ... for 4 days with complete EGM-2 media (Lonza CC-4147, Lonza CC-3156). Upon reaching 80-90% confluence ...
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bioRxiv - Cell Biology 2021Quote: ... immature RPE cells were seeded at a density of 1 × 105 cells/cm2 onto growth factor reduced ECMH (Corning)-coated transwells and allowed to be mature for 4-6 weeks in RPE medium (XVIVO 10, Lonza). Matured RPE at P1 or P2 were used in downstream experiments.
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bioRxiv - Microbiology 2023Quote: ... The RNA was loaded onto a 2% NuSieve 3:1 agarose (Lonza), 1X MOPS ...
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bioRxiv - Cancer Biology 2019Quote: ... were transfected using 4-D electroporator (LONZA), following manufacturer instruction ...
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bioRxiv - Immunology 2022Quote: ... and 4 mM of L-glutamine (Lonza). HLA-E expressing K562 cells were sorted and maintained in complete RPMI supplemented with 2 µg/mL blasticidin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... EMEM (ATCC; MRC-5 and Calu-3 cells) (Lonza; Tb-1 Lu cells) or MEM (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of eGFP-Centrin1 was achieved by electroporating 4.106 B lymphoma cells with 4 μg of plasmid using the Amaxa Cell Line Nucleofector Kit R (T-016 program, Lonza). Cells were cultured in CLICK medium for 5 to 16 h before imaging.
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bioRxiv - Cell Biology 2023Quote: ... 4 μM Alt-R Cas9 Electroporation enhancer (IDT) and 4 μM single-strand DNA homology template (Ultramer DNA Oligonucleotide, Lonza). After electroporation ...