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Citations for Lonza :
101 - 150 of 315 citations for 6 TERT BUTYLQUINOLINE 98.0+% GC T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... T cell pellets were resuspended in 20 uL per editing condition in P3 Buffer (Lonza) and then mixed with prepared RNP and DNA HDRT templates ...
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bioRxiv - Microbiology 2020Quote: ... or 6 days in growth factor-free Keratinocyte Basal Medium (Lonza) supplemented to achieve 1.5 mM [final] CaCl2.
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bioRxiv - Neuroscience 2021Quote: ... 6 hours post transfection cells were switched to Ultraculture medium (Lonza) and grown for another 24-48hrs ...
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bioRxiv - Immunology 2023Quote: ... and embedded in 6% w/v low melting point agarose (Lonza) in 1x PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... according to the manufacturer’s protocol using the program T-20 and the Amaxa kit R (Lonza). Cells were transfected with 1-3 nM non-targeting siRNA as a control (D-001810-10-05 ...
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bioRxiv - Bioengineering 2020Quote: ... cells were cultured in human T cell media comprised of X-VIVO 10 (Lonza #04-380Q), 5% human AB serum (Valley Biomedical #HP1022) ...
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bioRxiv - Biochemistry 2021Quote: ... and transiently transfecting with Amaxa® Cell Line Nucleofector® Kit T (Ref: VCA-1002, Lonza) two sets of sgRNAs (Hs.Cas9.ORAI1.1.AA Ref ...
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bioRxiv - Cell Biology 2020Quote: ... Jurkat T cells were transfected using the Amaxa Cell Line Nucleoefector Kit V (Lonza, VCA-1003). Transient transfections were performed as follows ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were electroporated using the T-028 program of a Nucleofactor™ 2b transfection device (Lonza). For the IFA analysis approximately 1/10th of the transfected cells were seeded in each well of a 24 well-plate ...
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bioRxiv - Bioengineering 2022Quote: ... 20 μL of T cells were resuspended in P3 buffer at 5 × 107 cells/mL (Lonza) and added to the electroporation mixture ...
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bioRxiv - Immunology 2022Quote: ... Cells were electroporated using program T-020 on the Amaxa 4D-Nucleofector (Lonza, Walkersville, MD, USA). After nucleofection ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were washed with PBS and rested in X-VIVO 15 (#BE02-060F, Lonza, Switzerland) at a density of 1 × 106 cells per mL at 37°C ...
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bioRxiv - Immunology 2023Quote: ... suspended in a concentration of 1.5×106 in 100 µl cell line nucleofector solution T (Lonza) and nucleofected with 5 μg of a plasmid using program X-001 in the Nucleofector IIb device (Lonza ...
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bioRxiv - Immunology 2023Quote: ... and T cells were electroporated and transfected with TRAC RNP using a 4D-Nucleofector device (Lonza) and P3 Primary Cell Solution according to manufacturer protocol (Lonza) ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated naïve CD8+ T-cells were cultured in X-Vivo 15 medium (Lonza Cat. #04-418Q) supplemented with 5% human serum ...
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bioRxiv - Systems Biology 2021Quote: ... and routinely passaged every 4∼6 days with Versene (EDTA) solution (Lonza). Selected iPSC clones were further validated for expression of pluripotency markers with immunostaining (Supplementary Fig ...
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bioRxiv - Systems Biology 2021Quote: ... and routinely passaged every 4~6 days with Versene (EDTA) solution (Lonza). Selected iPSC clones were further validated for expression of pluripotency markers with immunostaining (Chen et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... This mixture was nucleofected with 2×106 stimulated human primary T cells using the 4D-Nucleofector (Lonza) with the program EO-115 ...
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bioRxiv - Bioengineering 2021Quote: Landing pad cell lines were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with the promoterless mCherry donor containing the matching attP at a dose of either 1000 ...
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bioRxiv - Bioengineering 2021Quote: ... 1.0×106 WT K562 were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1 μg of PiggyBac expression vector (PB200A-1 ...
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bioRxiv - Bioengineering 2021Quote: 1.2×106 K562 cells were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 2000 ng of a pEF-1α-PuroR-P2A-mCherry donor plasmid containing an upstream Cp36 attD site (pJT371) ...
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bioRxiv - Bioengineering 2021Quote: ... 1.2×106 K562 cells were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 2000 ng of the same Cp36 PuroR-P2A-mCherry donor ...
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bioRxiv - Biophysics 2020Quote: ... were cultured in T-75 flasks in mammary epithelial basal medium (MEGM basal medium CC-3151; Lonza) that was supplemented with bovine pituitary extract ...
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bioRxiv - Microbiology 2019Quote: ... brucei using the Human T-cell nucleofection® kit and an Amaxa® Nucleofector™ (Lonza AG) set to program X-001 ...
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bioRxiv - Immunology 2020Quote: ... transferred to an electroporation cuvette and electroporated using program T-023 on an Amaxa Nucleofector (Lonza Bioscience). Cell were then left to recover for 24 to 48 hours in fresh RPMI-1640 media ...
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bioRxiv - Immunology 2019Quote: ... T cells were centrifuged for 10 min at 90x g and resuspended in electroporation buffer P3 (Lonza) at a concentration of 750,000 cells/ 20 μL ...
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bioRxiv - Immunology 2019Quote: ... 10×106 Jurkat T cells were electroporated using the P2 primary Cell 4D-nucleofector kit from Lonza with program EH-100 ...
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bioRxiv - Bioengineering 2021Quote: ... 1.2×106 K562 cells were electroporated in 100 μl Amaxa solution (Lonza Nucleofector 2b, program T-016), with 300 ng of the 11.6 kb LSR plasmid ...
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bioRxiv - Immunology 2021Quote: ... RNPs were electroporated immediately after complexing into Tregs and T cells resuspended in supplemented P3 buffer (Lonza).
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bioRxiv - Molecular Biology 2023Quote: ... according to the manufacturer’s instructions and the T-018 program of the Amaxa Nucleofector II device (Lonza). After nucleofection ...
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bioRxiv - Bioengineering 2023Quote: ... and CD8 T cells were purchased from the StemCell and cultured in X-VIVOTM 15 media (Lonza) supplied with 5 % human AB serum (MP Biomedicals ...
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bioRxiv - Cancer Biology 2023Quote: ... and T cells were added at different concentrations for 48 h in X-VIVOTM 15 media (Lonza) supplemented with 5 % human AB serum (Gemini Bio ...
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bioRxiv - Bioengineering 2023Quote: Nucleofection in T cells were performed using P3 Primary Cell 4D-Nucleofector™ X Kit S (Lonza) according to the manufacturer’s instruction with minor modifications ...
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bioRxiv - Immunology 2023Quote: ... 1e6 T cells were resuspended in 20 µl P3 electroporation buffer with P3 supplement (Lonza. #V4XP-3032) as per manufacturer instructions and combined with 3 µl RNP ...
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bioRxiv - Immunology 2024Quote: ... cells were washed once with PBS and resuspended in T cell nucleofection solution (Lonza, Cat: VPA-1002) containing plasmids of choice and nucleofected using the T-023 program on the Amaxa IIb nucleofector ...
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bioRxiv - Bioengineering 2024Quote: ... Electroporation kit V and Electroporation kits for Primary T-Cell/HSPC were purchased from Lonza (Basel, Switzerland) and used on the Lonza Nucleofector 2b system ...
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bioRxiv - Cancer Biology 2020Quote: ... Human umbilical vein endothelial cells (HUVECs) (up to passage 6; Lonza, Wokingham, UK) were cultured in EGM-2 medium (Lonza ...
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bioRxiv - Paleontology 2019Quote: ... and rib bones of a 6-year-old female (NHOst-Osteoblasts by Lonza Pharma ...
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bioRxiv - Cell Biology 2019Quote: ... and routinely (every 6 months) tested negative to mycoplasma (MycoAlert, LT07-218, Lonza).
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bioRxiv - Plant Biology 2023Quote: ... They were then embedded in 6% SeaKem® LE agarose (Lonza, Basel, Switzerland), and sections of 100 µm were realized using a vibrating blade microtome (Microm 650V ...
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bioRxiv - Cell Biology 2022Quote: Human embryonic kidney 293 T-antigen (HEK293T) cells (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Lonza) and supplemented with 10% fetal bovine serum (Thermo Fischer Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... and 2mM L-glutamine. Spinner-adapted L929 cells (obtained from T. Dermody’s laboratory) were maintained in Joklik’s MEM (Lonza) supplemented to contain 5% FBS ...
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bioRxiv - Microbiology 2019Quote: ... and 2 mM L-glutamine. Spinner-adapted L929 cells (obtained from T. Dermody’s laboratory) were maintained in Joklik’s MEM (Lonza) supplemented to contain 5% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were isolated from PBMCs as described and cultivated overnight in X-Vivo 20 (Lonza, Basel, Switzerland) supplemented with 10% human serum ...
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bioRxiv - Immunology 2019Quote: ... Lifeact-GFP was transiently transfected into primary T cell blasts using the Amaxa 96-well shuttle system (Lonza) and the recommended protocol for activated human T cells.
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bioRxiv - Immunology 2019Quote: ... To image T cells migrating randomly on immobilized ICAM-1 cells were resuspended in L-15 media (Lonza) supplemented with 6mM Glucose and allowed to adhere to ICAM-1 coated chambers ...
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bioRxiv - Microbiology 2020Quote: ... Nucleofection was performed using the program X-001 for Jurkat T-cells (Amaxa™ Nucleofector™ II, Lonza). Cells were cultured in RPMI-1640 medium media supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... 1 million Jurkat T-cells were resuspended in 100 μL Nucleofector solution (Cell Line Nucleofector™ Kits, Lonza) and combined with 2 μg of the linearized dual-fluorophore vector and 2 μg of the corresponding gRNA/Cas9 plasmid ...
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bioRxiv - Cell Biology 2020Quote: Jurkat T cells (clone E6.1; ATCC TIB-152) were grown in RPMI 1640 medium w/ L-Glutamine (Lonza) supplemented with 10% Fetal Bovine Serum and 1% penicillin/streptomycin ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... the siRNAs were transfected into human CD4+ T cells using the Amaxa Nucleofector following the manufacturer’s protocols (Lonza). The siRNAs used in this study were purchased from GenePharma and the sequences are as below: