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Citations for Lonza :
151 - 200 of 1203 citations for 6 Chlorotetrazolo 1 5 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The purified CD19+ B cells were cultured in the chemical-defined and serum-free X’VIVO 15 medium (Lonza, Walkersville, MD, USA), in the absence or presence of 100 U/ml penicillin ...
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bioRxiv - Systems Biology 2024Quote: ... MT2 and MDA-MB-231 cells with HLA-A,B,C CRISPR-Cas9 knockout were generated through electroporation of Cas9-sgRNA ribonucleoprotein (RNP) complex using 4D-Nucleofector (Lonza Bioscience). Three sgRNAs targeting HLA gene (HLA sgRNA#1 ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions were pooled and buffer-exchanged to phosphate-buffered saline (PBS; 140 mM NaCl, 5 mM NaH2PO4, 5 mM Na2HPO4, pH 7.4; Lonza, UK) using a Sephadex G-25 column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions were pooled and buffer-exchanged to phosphate-buffered saline (PBS; 140 mM NaCl, 5 mM NaH2PO4, 5 mM Na2HPO4, pH 7.4; Lonza, UK) using a Sephadex G-25 column (GE Healthcare ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were dissociated into single cells with accutase and 1 million cells were used for nucleofection with 5 μg of gRNA/Cas9 plasmid and 5 μg of each donor plasmid (WT and G12D) using Nucleofector II (Lonza) and program B-16 ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions were pooled and buffer-exchanged to phosphate-buffered saline (PBS; 140 mM NaCl, 5 mM NaH2PO4, 5 mM Na2HPO4, pH 7.4; Lonza, UK) using a Sephadex G-25 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Fractions were pooled and buffer-exchanged to phosphate-buffered saline (PBS; 140 mM NaCl, 5 mM NaH2PO4, 5 mM Na2HPO4, pH 7.4; Lonza, UK) using Sephadex G-25 media (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Fractions were pooled and buffer-exchanged to phosphate-buffered saline (PBS; 140 mM NaCl, 5 mM NaH2PO4, 5 mM Na2HPO4, pH 7.4; Lonza, UK) using a Sephadex G-25 column (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... the mammary glands were digested overnight at 37 ºC and 5% CO2 in a loosely capped 50 ml falcon with 5 ml of DMEM/F12 (Lonza) supplemented with 25 mM HEPES ...
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bioRxiv - Neuroscience 2024Quote: ... 2.5 × 10 5 phNPCs cells were used per electroporation reaction in one cuvette of the 16-well Nucleocuvette Stripe (Lonza). After trypsinization ...
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bioRxiv - Developmental Biology 2020Quote: ... completed with 5% fetal bovine serum (FBS, Lonza) and 2mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... 2% glutamine and 5% human serum AB (Lonza) for seven days ...
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bioRxiv - Cell Biology 2021Quote: ... 5% C02 in EGM-2 (Lonza, #CC-3162), media was changed every two days and cultured to passage 6 ...
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bioRxiv - Microbiology 2021Quote: ... and 10^6 cells were transfected into cells using Amaxa 96-well Nucleofector (Lonza, Basel, Switzerland) SF kit on program DS-138 ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... 6 × 106 macrophages were resuspended in 200 μL of Hams F10 complete media (Lonza, 12-618F) containing 10% horse serum (Gibco ...
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bioRxiv - Immunology 2021Quote: Commercially available human primary PTs from 6 donors (3 males and 3 females, Lonza Walkersville Inc) were expanded at passage 4 ...
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bioRxiv - Cancer Biology 2019Quote: ... while HUVEC cells were cultured for up to 6 passages in EGM-2 media (Bulletkit, Lonza) comprising all components ...
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bioRxiv - Biophysics 2020Quote: ... ∼300 pairs of ovaries were harvested from 3-6 day old females in 1X PBS (Lonza) with 1 mM DTT (Sigma ...
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bioRxiv - Immunology 2020Quote: ... Lymph nodes were embedded in 6 % w/v low melting point agarose (Lonza, Walkersville MD, USA) in 1X PBS ...
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bioRxiv - Biochemistry 2022Quote: ... For dual sgRNA gene editing 100 pmols of Cas9 RNP with cut site A sgRNA and 100 pmols of Cas9 RNP with cut site B sgRNA were nucleofected (Lonza 4D nucleofector) with CA137 pulse code ...
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bioRxiv - Cell Biology 2021Quote: ... and CRX +/tdtomato were maintained in reduced growth factor Matrigel (50ug/cm^2) in mTeSR1 plus with 1x Pen/Strep Amphotericin B (Lonza, 17-745E).
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bioRxiv - Immunology 2020Quote: ... 1 × 106 BLaER1 cells were transfected with 2 μg plasmid DNA using the Human B Cell Nucleofector Kit and Nucleofector I device (program U-15; both Lonza, Basel, Schweiz). On day 2 post transfection ...
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bioRxiv - Bioengineering 2023Quote: ... MCF-10A cells were cultured using an MEGM™ Mammary Epithelial Cell Growth Medium BulletKit™ without gentamycin-amphotericin B mix (Lonza) following ATCC’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... for 1.5 h at 37°C and 5% CO2 before seeded with human umbilical vein endothelial cells (HUVECs, pooled donor, Lonza, Switzerland). Endothelial Cell Growth Medium-2 (EGM-2 ...
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bioRxiv - Bioengineering 2019Quote: ... and 6 µg optoWnt donor plasmid in 200 µl room temperature PBS -/- using a NucleofectorTM 2b (Lonza) with program B-016 ...
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bioRxiv - Cell Biology 2021Quote: ... immature RPE cells were seeded at a density of 1 × 105 cells/cm2 onto growth factor reduced ECMH (Corning)-coated transwells and allowed to be mature for 4-6 weeks in RPE medium (XVIVO 10, Lonza). Matured RPE at P1 or P2 were used in downstream experiments.
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bioRxiv - Cancer Biology 2021Quote: ... Lack of mycoplasma contamination was confirmed every 6 months using MycoAlert mycoplasma detection kit (Lonza, #LT07-418).
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bioRxiv - Neuroscience 2023Quote: ... Four-day EBs were then differentiated in 6-well plates (15 EBs/well) in X-VIVO15 (Lonza) supplemented with 100 ng/ml M-CSF (Miltenyi Biotec) ...
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Cyborg islets: implanted flexible electronics reveal principles of human islet electrical maturationbioRxiv - Bioengineering 2024Quote: ... USA) were maintained in 6-well plates using MSCGM BulletKit medium (cat # PT-3238 & PT-4105, Lonza).
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bioRxiv - Biophysics 2021Quote: ... The WT and mutant genes were then amplified from these expression plasmids using the PCR primers 5’-GCGCTAGCAGCACCCATATGCCTGAG-3’and 5’-ACGAAGCTTTCAGTGGTGGTGGTGGT-3’and subcloned into the pMaxCloning vector (Lonza, VDC-1040) via NheI and BamHI sites to generate the pMax_WT_NEIL1 and pMax_Mutant_NEIL1 expression plasmids that were utilized throughout this analysis.
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bioRxiv - Bioengineering 2019Quote: ... supplemented with 5% fetal bovine serum (FBS) (Lonza, USA), EGF 20 ng/mL (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... treated with 5 ml of ACK lysing buffer (Lonza), and frozen with 3ml of Bambanker freezing media ...
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bioRxiv - Cell Biology 2022Quote: ... 5 μL of 200× SYBR Green I (Lonza, Switzerland) was added to 200 μL of the diluted culture for a final concentration of 5× SYBR Green I ...
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bioRxiv - Microbiology 2021Quote: ... treated with 5 ml of ACK lysing buffer (Lonza), and frozen with 3ml of Bambanker freezing media ...
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bioRxiv - Genetics 2022Quote: ... 5% CO2 in EGM-2 media (Lonza, Allendale, NJ). Cells were sub-cultured every 3-4 days based on confluence estimates of 70-90% with media exchanges occurring every 2 days ...
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bioRxiv - Microbiology 2022Quote: ... treated with 5 ml of ACK lysing buffer (Lonza), and frozen with 3 ml of Bambanker freezing media ...
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bioRxiv - Cancer Biology 2023Quote: ... incubated with 5 ml of 0.5 mM EDTA (Lonza) at 37° C for 2 minutes to detach the cells ...
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bioRxiv - Biophysics 2023Quote: ... and in 5 mL culture medium (SmGM-2, Lonza) at last ...
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bioRxiv - Bioengineering 2023Quote: ... 1X (5 mL) of L-glutamine (Lonza, 17-605E), and 1X (5 mL ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were seeded in 6 well plates and grown to ∼80% confluency in DMEM (Lonza, 12-614F) with P/S ...
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bioRxiv - Biochemistry 2021Quote: ∼300 pairs of ovaries per replicate were harvested from 3-6 day old females in 1X PBS (Lonza) with 1 mM DTT (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: Inguinal white adipose tissue from 3-4 WT-C57Bl/6 male mice was collected and placed in Dulbecco’s modified eagle’s medium (DMEM; Lonza) supplemented with 1% Penicillin/Streptomycin (PS ...
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bioRxiv - Molecular Biology 2022Quote: ... HUVECs were prepared in 6-well plates with 2.8.105 cells/well in EGM-2 medium (Endothelial Cell Growth Medium-2, Lonza). Cells were transduced in duplicate with 20 MOI (Multiplicity of Infection ...
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bioRxiv - Molecular Biology 2023Quote: ... 6-well dishes were seeded with 1.0 × 106 Sf9 cells per well in 2.0 mL InsectExpress medium (Lonza). Cells were transfected with 20 mL of bacmid per well ...
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bioRxiv - Neuroscience 2020Quote: ... 6 µg of total plasmid DNA (2 µg for each of the three episomal vectors) were electroporated into 5×10^5 fibroblasts with the Amaxaμ Human Dermal Fibroblast Nucleofectorμ Kit (Lonza, Basel, Switzerland; VDP-1001). The fibroblasts were further cultured for 6 days to allow them to recover ...
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bioRxiv - Bioengineering 2021Quote: ... 5 % CO2 incubator in SmGM− growth medium (Lonza, Basel, Switzerland). The SMCs were passaged at 70 – 80 % confluency ...
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bioRxiv - Bioengineering 2020Quote: ... and P3-5 human dermal fibroblasts (HDFs, Lonza CC-2509) were dissociated ...
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bioRxiv - Developmental Biology 2020Quote: Passage 5 Human Umbilical Vein ECs (HUVECS) (Lonza, CC-2517) were used for the in vitro experiments ...
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bioRxiv - Immunology 2021Quote: ... or an electroporation cartridge (100 µl = 5×106 cells, Lonza). Cells were carefully transferred onto the electroporation strips using 200 µl tips to avoid trapping air in the solution ...
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bioRxiv - Bioengineering 2022Quote: ... 5% CO2 in EGM-2 Bullet Kit (Lonza CC-3162) medium and used between passages 4-8.