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Citations for Lonza :
401 - 450 of 608 citations for BN80933 CAS 214348 10 4 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 1% 10 mM HEPES in 0.85% NaCl (17-737E, Lonza, BioWhittaker®, Walkersville, MD, USA), 1% antibiotic-antimycotic ...
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bioRxiv - Microbiology 2024Quote: ... 6 - 10 µg RNA per lane was first resolved on a 1.2% agarose gel (Lonza) in 1 x MOPS buffer (Quality Biological ...
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bioRxiv - Cancer Biology 2024Quote: ... p.Val560Asp) (RRID:CVCL_7041) (Bauer et al, 2006) cells were maintained in Ham’s F-10 media (Lonza) supplemented with 15% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were electroporated with enhancer (IDT, #1075915, final concentration 4 μM) in a nucleofector device (Lonza, Nucleofector 2; program D-032). Cells were incubated for 24 h to allow them to recover and then detached and cloned by serial dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Human primary hepatic stellate cells from donors 3 and 4 were purchased as isolated hepatic stellate cells from Lonza (cat# HUCLS). Donor information is listed below.
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bioRxiv - Genomics 2021Quote: The MPRA libraries were electroporated in 4 to 6 million cells each using the Nucleofector 2b or 4D (Lonza, Basel, Switzerland) with 6 µg of plasmid DNA and program T-030 or Y-001 and DS-132 ...
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bioRxiv - Physiology 2022Quote: ... 5 million cells were washed with phosphate buffered saline (PBS) and electroporated with 4-6 μg of appropriate plasmid construct using an Amaxa cell nucleofector (Lonza laboratories) and nucleofection reagent (362.88 mM ATP-disodium salt ...
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bioRxiv - Neuroscience 2023Quote: ... Nucleofection was performed using the 4-D nucleofector system (AMAXA) and the P3 Primary Cell 4D-Nucleofector Kit (Lonza, V4XP-3024) following manufactures’ instructions (program CB-150) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were grown under sterile conditions for no longer than 4 weeks after thawing and were frequently tested for Mycoplasma using the MycoAlert® Assay kit (Lonza).
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bioRxiv - Cell Biology 2024Quote: ... DCs derived from progenitor cells were transfected with 4 μg of DNA using the nucleofector kit for primary T cells (Amaxa, Lonza Group) following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2020Quote: ... 106 cells per condition were nucleofected with 4 µg of DNA using the Amaxa Nucleofector II (Lonza, Kit R; program R-024). Nucleofected cells were allowed to recover for 24-48 hours before sorting for GFP positivity and lack of CD56 expression.
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bioRxiv - Immunology 2020Quote: ... Transfection of primary T cells with Cas9 RNP complexes and TCRβα HDR templates was performed 3-4 days following activation using the 4D-Nucleofector and a 20 uL format P3 Primary Cell kit (Lonza, V4XP-3032). Briefly ...
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bioRxiv - Immunology 2022Quote: ... puromycin containing medium were changed and every 4 days cells were fed EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ (LONZA) supplemented with GlutaMax (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were electroporated at 5 million cells/100 mL cells per cuvette using a Lonza 4-D nucleofector with pulsecode DP-148 (Lonza, VVPA-1002). Cells were co-cultured for 5 additional days with human astrocyte before transferring cells to homeostatic culture conditions (MGdM media ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... droplets in 6 well plates and treated with retinoic acid for 4 days followed by hepatocyte growth media (Hepatocyte Culture Medium BulletKit (Lonza, CC-3198) supplemented with 10 ng/mL hepatocyte growth factor (PeproTech ...
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bioRxiv - Bioengineering 2024Quote: ... RNP’s and DNA were electroporated (EP) into T-cells in 16 well EP cuvettes on a 4-D Nucleofector X unit (Cat # V4XP-3032, Lonza, Walkersville, VA) using pulse code EH-115 ...
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bioRxiv - Microbiology 2021Quote: ... and 10^6 cells were transfected into cells using Amaxa 96-well Nucleofector (Lonza, Basel, Switzerland) SF kit on program DS-138 ...
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bioRxiv - Immunology 2021Quote: ... NIH) in 100 µl IMDM/Glutamax/10% FBS containing 2× MycoZap Plus-PR (Lonza #VZA-2021), 100 ng/ml human IL-2 (GoldBio #1110-02-50) ...
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bioRxiv - Bioengineering 2020Quote: ... cells were cultured in human T cell media comprised of X-VIVO 10 (Lonza #04-380Q), 5% human AB serum (Valley Biomedical #HP1022) ...
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bioRxiv - Bioengineering 2021Quote: ... 100M PBMCs were thawed in 100 mL X-VIVO™ 10 media from Lonza (#04-380Q) with recombinant human IL-2 protein from R&D Systems (#202-IL) ...
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bioRxiv - Cancer Biology 2022Quote: ... RBCs were lysed in 1X red blood cell lysis buffer ACK Lysis Buffer (Lonza 10-548E). Either CD45 magnetic beads or T cell isolation kit (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2020Quote: Single cells were suspended in X-VIVO 10 Serum-free Hematopoietic Cell Medium (#04-380Q, Lonza) in six-well Ultra-Low Attachment Plates (Corning) ...
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bioRxiv - Immunology 2021Quote: ... Isolated neutrophils were cleared off residual red blood cells by ACK lysis buffer (10-548E, Lonza) and resuspended in mHBSS buffer (20mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: ... and the cell pellet resuspended in 2 ml of ACK lysing buffer (Lonza, Cat# 10-548E). After centrifugation ...
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bioRxiv - Systems Biology 2024Quote: ... supplemented with 10% fetal bovine serum (ATCC) and 0.2% MycoZap Plus-CL antibiotics (Lonza, catalog # 195261). Cell lines were routinely tested for mycoplasma and were periodically authenticated to its early passage using the GenePrint 10 System (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... containing 10 µg of Cas9 or Cas9/guide plasmidic DNA and transferred to a cuvette (Lonza). Cells were nucleofected with the DT-130 program using the 4D-Nucleofector system with Core and X unit (Lonza) ...
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bioRxiv - Immunology 2024Quote: ... NIH) in 100 µL IMDM GlutaMAX/ 10% FBS containing 2× MycoZap Plus-PR (Lonza #VZA-2021), 100 ng/mL human IL-2 (GoldBio #1110-02-50) ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% FBS and 1% antibiotic-antimycotic and endothelial cell growth supplement (#CC-4176, Lonza) to 80% confluency before passaging (up to passage number 8) ...
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bioRxiv - Immunology 2023Quote: ... NIH) in 100 µl IMDM/Glutamax/ 10% FBS containing 2× MycoZap Plus-PR (Lonza #VZA-2021), 100 ng/ml human IL-2 (GoldBio #1110-02-50) ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 x 106 hiPSCs were resuspended in 100 µl P3 solution with supplement (Lonza, #V4XP-3024) and transfected with 2.5 µg linearized RUNX1 HDR template and 4 µg each of pCW-Cas9 plasmid (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... and transfected into 10 million cells using a NucleofectorTM 2b Device and program T-029 (Lonza). Cells were plated into 12-well plates and the media was changed after 24 hr ...
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bioRxiv - Cancer Biology 2024Quote: ... cell pellets were resuspended in two to three volumes of ACK lysis buffer (Lonza: #10-548E) according to the manufacturers’ specifications ...
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bioRxiv - Immunology 2024Quote: ... red blood cells were removed using Ammonium-Chloride-Potassium (ACK) lysing buffer (10-548E; Lonza Bioscience). The cell pellet was resuspended in separation buffer (PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... and delivered into 10 million vAbl cells using 4D-nucleofector system (Lonza, Core plus X unit).
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bioRxiv - Neuroscience 2024Quote: BMDM were differentiated in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with heat-inactivated FBS (10%, Lonza), penicillin/streptomycin (100 U/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% fetal bovine serum (FBS; Dutscher, Bernolsheim, France) and antibiotics (penicillin and streptomycin; LONZA). The cultures were maintained at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... MCF-10A human mammary gland cells were used as the non-tumorigenic control and were cultured in MEBM basal medium supplemented with components included in the MEGMTM Mammary Epithelial Cell Growth Medium SingleQuotsTM Kit (Lonza, Hayward, CA). A549 human lung carcinoma cells were cultured in Dulbecco’s Modified Eagles Medium (DMEM ...
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bioRxiv - Cell Biology 2024Quote: All eight guide plasmids (4 for Sun1 and 4 for Sun2) were transfected into A549 cells using the Amaxa Cell Line Nucleofector Kit T (Lonza Bioscience, VCA-1002) according to the manufacturer’s instructions and plated into a 10cm plate in A549 media ...
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bioRxiv - Immunology 2021Quote: ... 1-10 million activated P14 cells were re-suspended in 20 μL primary cell nucleofection solution (Lonza), then mixed and incubated with the crRNA/Cas9 mix for 2 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... CMPs and MEPs were cultured for 36-48 hours in serum-free X-VIVO 10 media (Lonza) supplemented with 1% Bovine Serum Albumin Fraction V (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... U2OS and vinculin-/- MEFs were cultured in Dulbecco’s modified Eagles medium (DMEM) supplemented with 10% FCS (Lonza), 1% L-glutamine (Sigma) ...
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bioRxiv - Genetics 2022Quote: ... 10 μg of Cas9 twin guide construct was nucleofected in GC1 cells using 4D-Nucleofector (LONZA, Switzerland) and after growing them for 24 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were passaged 5-10 times and periodically tested for contamination using MycoAlert™ Mycoplasma Detection (Lonza) kit.
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bioRxiv - Microbiology 2021Quote: ... knowlesi parasites were enriched using Histodenz and 10 μl of schizonts mixed in a transfection cuvette (Lonza) with 100 μl of P3 solution (Lonza ...
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bioRxiv - Cancer Biology 2023Quote: ... Both bone marrow and mammary gland were treated with ACK Lysing Buffer (10-548E; Lonza, Basel, Switzerland) to lyse red blood cells ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% FBS (Hyclone) and Microvascular Endothelial Growth Medium growth factor kits (Lonza Cat# CC-4147). HLMVECs between passages 5 and 9 were used for experiments ...
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bioRxiv - Bioengineering 2024Quote: ... Suspensions were centrifuged for 10 minutes at 300g and digested with ACK lysing buffer (Lonza, Walkerville, VA). The cells were then washed with PBS ...
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bioRxiv - Microbiology 2023Quote: ... BHK-21 Clone 13 (ATCC number CCL-10) were cultured in Glasgow Minimum essential medium (GMEM, Lonza) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.3) containing 10 μg DNA and transfected by electroporation using an AMAXA Nucleofector® Device (Lonza) with program “X-001 free choice” ...
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bioRxiv - Neuroscience 2022Quote: ... and 10 μg plasmid DNA in a volume of 25-30 μL of AD1 Solution (Lonza Bioscience) was put as a drop on the slice ...