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Citations for Lonza :
351 - 400 of 408 citations for Persistent Pesticide Calibration Solution Cs1 Cs8 Unlabeled 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... The complex was then transferred to a 20 μl single-cell suspension of 2 × 105 hESCs in P3 nucleofection solution and electroporated using Amaxa 4D-Nucleofector™ (Lonza) with program CA137 ...
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bioRxiv - Bioengineering 2021Quote: ... we nucleofected HEK293FT-RfxCas13d cells with 225 μmol of synthetic crRNAs in 20μl reaction of SF Cell Line Nucleofector Solution (Lonza V4XC-2032) using the Lonza Nucleofector 4D (program CM-130) ...
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bioRxiv - Cancer Biology 2020Quote: ... dissociated into single cell suspension with trypsin-EDTA (Pan-Biotech, Germany) for 3 min followed by trypsin neutralizing solution (Lonza, Germany). Single cell suspensions of secondary mammospheres were obtained as described for day 7-first generation mammospheres.
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bioRxiv - Neuroscience 2022Quote: We prepared thin agar pad for the samples where 8 µL of semi-cooled 1.5% agarose solution (add low melting temperature NuSieve GTG Agarose (Lonza, Cat# 50081) to 2% galactose media (0.77 g of CSM-URA (Sunrise # 1004-010) ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended with 10µL of primary cell nucleofection solution (P3 primary Cell 4D-Nucleofector X kit S (Lonza, # V4XP-3032), and mixed with RNP complexes ...
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bioRxiv - Microbiology 2022Quote: ... Dissociated cells were aspirated using a wide-bore pipette tip and to a tube containing ice-cold Trypsin Neutralization Solution (Lonza, CC5002); this was repeated to maximize cell collection ...
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bioRxiv - Biochemistry 2019Quote: ... 1.5-2×106 cells were pelleted for each condition and resuspended in 100 μL complete nucleofector solution (SE Cell Line 4D-Nucleofector™ X Kit, Lonza) to which 1μg of pCR2.1-mClover-LMNAdonor ...
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bioRxiv - Developmental Biology 2020Quote: ... or a PMO Control at 100 µM in 100 µL solution from the P3 Primary Cell 4D-Nucleofector® X Kit L (V4XP-3024, Lonza) using the CB150 program on the 4D-Nucleofector™ System (Lonza) ...
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bioRxiv - Immunology 2021Quote: ... were washed in PBS/0.5%BSA and resuspended in 100 µl Nucleofector solution (Amaxa™ P3 Primary Cell 4D Nucleofector TM X Kit L, Lonza) with 4-6 µl of the appropriate siRNA (20-40 µM ...
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bioRxiv - Genomics 2022Quote: ... We used 1.0 × 106 HCT116 or U2OS cells to transfect the base editor plasmids (2600 ng) using SE solution and 4D-nucleofector (Lonza, Switzerland) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Sections attached to slides were fixed with cold acetone/ethanol (1:1) solution and washed in PBS buffer (Lonza, Morristown, NJ). Primary antibodies and isotype controls (Supplementary Table 9 ...
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bioRxiv - Molecular Biology 2022Quote: ... collected by centrifugation at 500xg for 5 min and resuspended in SF solution (SF cell line 4D-Nucleofector™ X Kit, Lonza). After addition of the RNP ...
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bioRxiv - Genomics 2023Quote: ... and 120 pmol of in vitro synthesized gRNA were electroporated into 250,000 MCF7 cells with the P3 primary nucleofection solution (Lonza, V4XP-3024), using the DN-100 Lonza 4D-Nucleofector program ...
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bioRxiv - Genetics 2023Quote: All iPSC lines were passaged as clumps or single cells upon reaching 80-85% confluency using Versene (0.02% EDTA solution, Lonza #17-711E) or StemPro Accutase (Gibco #A1110501) ...
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bioRxiv - Biophysics 2023Quote: ... 1.5mL for a T75 flask for 5 minutes at 37° C and resuspended in 80 μL of SF cell line solution (Lonza; Basel) per flask ...
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bioRxiv - Microbiology 2019Quote: ... Cells were resuspended in 25 uL of CRISPR/Cas9 crRNP complexes that were pre-assembled in P3 Primary Cell Nucleofector Solution (Lonza #V4SP-3096) before electroporation in a single well of a 96-well Nucleocuvette Plate using program EH-115 ...
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bioRxiv - Physiology 2021Quote: ... were mixed with 1.49 μL of ssODN (100 μM) and Cas9 complex consisting of 18 μL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 μL of sgRNA (30 pmol/μL) ...
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bioRxiv - Microbiology 2022Quote: ... Cells that were harvested at 48 hours were first washed by dispensing and aspirating 37°C HEPES buffered saline solution (Lonza, CC-5022), and then trypsinized with 0.025% Trypsin/EDTA (Lonza ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% (v/v) fetal bovine serum (FBS, Biochrom AG, Berlin, Germany) and 1% (v/v) antibiotic–antimycotic solution (AAS, Lonza, Basel, Switzerland) was used for culturing human dermal fibroblasts (HDF ...
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bioRxiv - Immunology 2020Quote: ... 50 pmol sgRNA and 20 pmol Cas9 were combined and diluted to 20 µL with nucleofection solution P1 or P3 (with supplement added, Lonza, Basel, Switzerland). The mixture was incubated at 25 °C for 10 min or up to 2 h and immediately used to electroporate moDCs ...
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bioRxiv - Genomics 2021Quote: ... mESCs in 6-cm dishes were transfected with 7.2 μg pX330-Cas9 plus 1.8 μg GFP expression vector by 4D-nucleofector X (Lonza, solution Cytomix, program GC104), then harvested for genomic DNA 3 days after transfection.
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bioRxiv - Neuroscience 2020Quote: ... dissociated ganglia were pelleted at 100 x g for 5 min and resuspended in 100 µl ‘Nucleofector solution’ (Rat Neuron Nucleofector kit; Lonza, Alpharetta, GA). 4-6 μg of plasmid was electroporated using the AMAXA Nucleofector device (Neurons Rat DRG ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels were fabricated using a solution consisting of lyophilized GelMA (5 wt%) dissolved at 37°C in phosphate buffered saline (PBS; Lonza 17-516F) and combined with 0.1% w/v lithium acylphosphinate (LAP ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 × 105 single cells were resuspended with Lonza P3 Primary Cell Nucleofector® Solution and mixed with the pre-formed RNP complexes and transferred to a Nucleocuvette™ (Lonza). Nucleofection was performed using program EO-115 on the 4D Nucleofector™ X unit (Lonza) ...
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bioRxiv - Molecular Biology 2022Quote: ... 480 pmol L2 gRNA targeting BFP and 500 pmol BFP to GFP ssODN template using CM-189 and SF solution (Lonza, 4D electroporator) per replicate ...
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bioRxiv - Cell Biology 2023Quote: ... The linearized construct and the purified schizonts were mixed with Nucleofector solution from an Amaxa human T cell Nucleofector Kit and electroporated using the Amaxa Nucleofector II device (Lonza, Köln, Germany). Directly after transfection 50 µl RPMI-1640 complete was added to the transfection reaction followed by intravenous injection into one SWISS mouse ...
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bioRxiv - Systems Biology 2024Quote: ... Dissociated cells were aspirated using a wide-bore pipette tip and placed in a tube containing ice-cold Trypsin Neutralization Solution (Lonza, CC-5002); this was repeated to maximize cell collection ...
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bioRxiv - Microbiology 2023Quote: Colons from infected mice were open longitudinally and gently washed with ice-cold 1X PBS before being placed in Hanks’ balanced salt solution (HBSS) with phenol red and without calcium and magnesium (Lonza, Basel, Switzerland) on ice ...
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bioRxiv - Genomics 2023Quote: ... were washed twice with PBS and resuspended in a solution containing a 4.5:5 mixture of nucleofection buffer (Buffer SE, Lonza Lot #: S-09279) and supplement (Lonza ...
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bioRxiv - Molecular Biology 2023Quote: ... were mixed with 1.49 µL of ssODN (100 µM) and Cas9 complex consisting of 18 µL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 µL of sgRNA (30 pmol/µL) ...
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bioRxiv - Cell Biology 2023Quote: ... RPE1 cell pellets containing 1×10^6 cells were resuspended in Nucleofection Solution for Primary Mammalian Epithelial Cells (Lonza cat# VPI-1005) in the presence of nucleofection enhancer (cat# 1075915 ...
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bioRxiv - Immunology 2023Quote: ... T cells were then rinsed with PBS and 10 × 106 cells were resuspended in 20 µl of P4 primary cell nucleofection solution (P4 Primary Cell 4D-Nucleofector X Kit S, Lonza V4XP-4032). 20 μL of resuspended T cells were then gently mixed with 5 µL of RNP complex and incubated for 2 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then passaged as previously described and 8×105 cells were resuspended in 100 µl of nucleofector solution (Lonza, VPH-5012). Depending on the experiment ...
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bioRxiv - Biochemistry 2024Quote: 1 µL of 63 µM Alt-R™ S.p.Cas9 V3 (IDT: 10007807) was diluted with 18 µL of SE Cell Line Nucleofector® Solution (Lonza V4XC-1032), followed by the addition of 1.8 µL of 100 µM sgRNA (3:1 sgRNA:Cas9) ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 1.0*106 iPSCs were gathered as single cells as described under ‘cell lines and cell culture’ and resuspended in 100 μL NucleofectorTM Solution V (Lonza, Basel, Switzerland) and added to the reaction mixture ...
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bioRxiv - Developmental Biology 2024Quote: ... cell pellets of 200x103 cells were resuspended with 20 µL of supplemented nucleofector solution P3 Primary Cell 4D-Nucleofector® S (Lonza, V4XP3032) with indicated amounts of multi-guide sgRNAs (Synthego ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were resuspended in 20 µl nucleofection buffer (16.4 µl Nucleofector® Solution + 3.6 µl Supplement) provided in P3 Primary Cell 4D-Nucleofector™ X Kit S (Lonza, V4XP-3032). After the addition of 3 µl RNP and 0.5 µl of Alt-R Cas9 Electroporation Enhancer (IDT ...
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bioRxiv - Cell Biology 2024Quote: ... and sgRNAs (see Key Resources Table) were delivered by nucleofection to a single cell suspension of 105 iBCs in Primary Cell P3 solution (Lonza, #V4XP-3032). Cells were re-cultured and the resulting clonally-derived spheroids were isolated ...
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bioRxiv - Microbiology 2024Quote: ... 5 million parasites were electroporated with 5-10ug of digested plasmid with an AMAXA Nucleofector II using X-001 in Human T-cell Nucleofector Solution (Lonza VPA-1002).
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bioRxiv - Cell Biology 2021Quote: ... The solution was transferred into a certified cuvette and cell electroporation was conducted using Nucleofector® program X-013 (Amaxa® Nucleofector®, Lonza). Electroporated cells were immediately resuspended in pre-warmed phenol-free Leibovitz’s-L-15 medium (Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... 1 × 106 cells were resuspended in 100 μl of the nucleofection hESC solution 1 (Human Stem Cell Nucleofector® Kit 1/ Lonza #VPH-5012) where 5 μg of plasmids were added previously ...
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bioRxiv - Cancer Biology 2020Quote: ... A total of 1.5×106 tumor cells (td-tomato MDA-MB-231 SORE6>GFP) were transfected with 10 μl of 20 μM siRNA stock solution using Nucleofector Kit V from Lonza (cat# VCA-1003) for 48 h ...
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bioRxiv - Genomics 2021Quote: ... Growing CH12F3 cells were transfected with 1.5 μg pX330-Cas9 or pX330-Cas9-mCherry expression vector per million by 4D-nucleofector X (Lonza, solution M1, procedure DN100) and seeding at 0.5 million cells/mL in fresh medium with 1μg/mL anti-CD40 ...
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bioRxiv - Microbiology 2022Quote: ... Each pellet was carefully resuspended in 20 μL of SF Cell Line Nucleofector Solution with Supplement 1 (Lonza Cat. #V4XC-2032, Basel, Switzerland) and 1.5 μL of pX458 plasmids (150 ng each of BAX g2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell pellets of 1E6 cells were resuspended with 20ul supplemented nucleofector solution of the P3 Primary Cell 4D-nucleofector® X Kit S (Lonza, V4XP-3032) and 2ug plasmid (1ug/ul) ...
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bioRxiv - Cell Biology 2023Quote: ... Next 1 × 106 cells resuspended in 93 μL of P3 Primary Cell Nucleofector Solution (P3 Primary Cell 4D-Nucleofector X Kit, Lonza, Catalog #: V4XP-3024) were mixed with 7 μL of RNP and 12 μg of single-stranded oligodeoxynucleotide (ssODN ...
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bioRxiv - Cancer Biology 2023Quote: ... The RNP complex was mixed with 8 x 10e5 GBM1 cells resuspended in 100 μl of SG solution from the SG Cell Line 4D-NucleofectorTM X Kit (Lonza, Cat# V4XC-3024). This entire mix was then placed in a NucleocuvetteTM and was nucleofected using a Lonza Amaxa 4D-Nucleofector TM and the EN138 nucleofection programme ...
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bioRxiv - Bioengineering 2021Quote: ... We then electroporated 4.0×105 of wildtype or the stably integrated mCherry K562 cell lines in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with pJT396 in combination with an equimolar amount of either pUC19 or a Cp36 expression vector ...
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bioRxiv - Bioengineering 2021Quote: ... were subsequently integrated into the AAVS1 locus of cells expressing rTetR-VP48 using TALEN-mediated homology-directed repair as follows: 1.0×106 K562 cells were electroporated in Amaxa solution (Lonza Nucleofector 2b, setting T0-16) with 1000 ng of reporter and 500 ng of each TALEN-L (Addgene #35431 ...
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bioRxiv - Cell Biology 2021Quote: MLIV patient fibroblasts were grown for 2-3 days until 80-90% confluent at 37°C and prepared for electroporation with approximately 50nM of ON-TARGETplus siRNA SMARTpool reagents from Horizon Cell Solutions (Dharmacon) according to manufacturer instructions using the Amaxa P2 Primary Cell 4D-Nucleofector X Kit (Lonza, Cat. No. V4XP-2032) with nucleocuvette strips in a Lonza 4D-Nucleofector X Unit ...