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Citations for Lonza :
351 - 400 of 568 citations for L Phenylalanine N T Boc 13C9 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... UK) were cultured in RPMI 1640 medium with 2mM L-Glutamine (Lonza, Belgium), supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... pulse CB-150 and Primary Cell 4D-Nucleofector Kit L (V4XP-3024, Lonza). Transfected cells were seeded at clonal density in dishes coated with hESC-qualified Matrigel (Corning Life Sciences ...
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bioRxiv - Microbiology 2023Quote: Atlantic salmon kidney (ASK) cells [51] were maintained in L-15 medium (Lonza) supplemented with foetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... cells were cultured in 4.5 g/L glucose containing DMEM (BE12-614F, LONZA), supplemented with 10% fetal bovine serum (10500-064 ...
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bioRxiv - Genomics 2023Quote: ... using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza, V4XP-3034) and the CA-137 program ...
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bioRxiv - Cancer Biology 2024Quote: ... Protocols according to the SG Cell Line 4D-Nucleofector X Kit L (Lonza) were followed ...
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bioRxiv - Biophysics 2023Quote: ... BSC-1 l cell lines were tested for potential mycoplasma contamination (MycoAlert, Lonza), and all tests showed negative results ...
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bioRxiv - Microbiology 2021Quote: ... while HO-1-N-1 (JCRB0831) cells were cultured in DMEM/F-12 (Dulbecco’s Modified Eagle Medium:F12; Lonza) medium ...
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bioRxiv - Immunology 2021Quote: ... The virus was propagated in the human neuroblastoma cell line SK-N-SH in Eagle’s Minimum Essential Medium (EMEM) with Earle’s Balanced Salt Solution (EBSS) (Lonza), supplemented with 10% (v/v ...
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bioRxiv - Immunology 2021Quote: ... Murine T cells were electroporated using the DN110 program of 4D nucleofector (4D-Nucleofector Core Unit: Lonza, AAF-1002B), human CAR T cells using the program E0115 ...
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bioRxiv - Microbiology 2021Quote: ... Pre-assembled Cas9-gRNA RNPs were electroporated into cells using Lonza Human T cell Nucleofector kit (Lonza, VPA-1002) and the Nucleofector2b equipment ...
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bioRxiv - Bioengineering 2019Quote: ... Transfection of transposon and transposase vectors was performed on day 2 with 0,5 µg transposon MC and 2 µg SB100X transposase RNA per 1×106 T cells on a 4D-Nucleofector according to the manufacturer’s instructions (Lonza). Transfected CD8+ and CD4+ T cells were expanded using the G-Rex culture system (Wilson-Wolf) ...
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bioRxiv - Immunology 2021Quote: ... On day 7 cells were nucleofected using the Amaxa Mouse T Cell Nucleofector Kit (Lonza, Cat no. VVPA-1006) per the manufacturer’s instructions with 5x106 cells and 1.5µg plasmid DNA per cuvette ...
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bioRxiv - Genetics 2020Quote: Vero E6 cells were cultured in T-175 flasks in Dulbecco’s modified Eagle’s medium (DMEM; Lonza, cat. 12-604F), supplemented with 8% fetal calf serum (FCS ...
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bioRxiv - Genetics 2019Quote: PBT cells were transfected with 5 μg DNA for 5.106 cells in 100 μl of Human T Cell Nucleofector Solution (Lonza) with the program U-014 (Amaxa Biosystems).
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bioRxiv - Immunology 2021Quote: ... Naive CD4+ T cells were cultured at 5% CO2/37°C in serum-free X-Vivo 15 medium (Lonza).
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bioRxiv - Cancer Biology 2022Quote: ... Both B-ALL and CAR-T cells were prepared for transplantation by resuspending in 200 μL Hank’s balanced salt solution (Lonza) and loaded in 27.5-gauge syringes (Becton Dickinson) ...
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bioRxiv - Immunology 2022Quote: ... 1 x 106 T-cells were pelleted and re-suspended in 100 μL nucleofection solution (Lonza, Walkersville, MD, USA). T-cell suspensions were mixed with 5 μl RNP complex and cell/RNP mix transferred to Nucleofector cuvette ...
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bioRxiv - Immunology 2023Quote: moDCs were cultured with allogenic naïve CD4+ T cells in a 1:10 ratio in complete IMDM (IMDM (BE12- 722F, Lonza) with 100 U/mL penicillin ...
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bioRxiv - Synthetic Biology 2024Quote: ... Isolated T cells were cultured at an initial density of 106 cells/mL in X-Vivo 15 medium (Lonza) supplemented with human serum (5% ...
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bioRxiv - Immunology 2023Quote: ... The precomplexed HDR-RNP complex was electroporated into primary human T cells as described above using P3 buffer (Lonza). The T cells were cultured in T cell media with 300 IU/ml IL-2 for 7-10 days ...
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bioRxiv - Microbiology 2020Quote: ... Infected CD4 T cells were washed with PBS and 3 million cells per condition were resuspended in 20uL buffer P2 (Lonza) with 4μM IDT electroporation enhancer ...
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bioRxiv - Bioengineering 2021Quote: ... 1.0×106 clonal Kp03 landing pad cells were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1 μg of PiggyBac expression vector (PB200A-1 ...
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bioRxiv - Genetics 2020Quote: ... All nucleofections were performed using 106 freshly-purified CD4 T cells in 100 μl nucleofection buffer using a Nucleofector 2b device (Lonza), based on previous optimisation studies (data not shown) ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of eGFP-Centrin1 was achieved by electroporating 4.106 B lymphoma cells with 4 μg of plasmid using the Amaxa Cell Line Nucleofector Kit R (T-016 program, Lonza). Cells were cultured in CLICK medium for 5 to 16 h before imaging.
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bioRxiv - Cancer Biology 2022Quote: ... siControl and siIRAK4 were purchased from Horizon Discovery and transfected into human AML samples using Amaxa Nucleofector Kit T (Lonza) (program number G-016 ...
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bioRxiv - Immunology 2022Quote: ... was used to enrich in CD4+ or CD8+ T cells from PBMCs that were cultured in X-vivo 15 media (Lonza) supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... Knockdown of Vps4a/b was performed using Amaxa Nucleofector II and the Mouse T-Cell Nucleofector Kit (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stimulated by CD3/CD28 Abs for 2 days and nuclofected with 100 μM RORC or non-targeting (NT1) siRNA (ON-TARGETplus SMART pool, Dharmacon) using the Amaxa Human T cell Nucleofector Kit (Amaxa, Lonza), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µg RNA was electroporated into BHK-21 cells using an Amaxa nucleofector 2b (program A-031) and Nucleofection T solution kit (Lonza). Transfected BHK-21 cells were mixed in a 1:1 ratio with cells susceptible to CoV infection ...
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bioRxiv - Microbiology 2020Quote: ... 5 μg of RNA was electroporated into 5×106 BHK-21 cells using the Amaxa nucleofector 2b (program A-031) and Nucleofection T solution kit (Lonza). Transfected BHK-21 cells were mixed with HuH7 or Vero cells in a 1:1 ratio and plated for harvesting supernatants ...
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bioRxiv - Genomics 2020Quote: ... bulk PBMCs obtained from healthy donors were thawed and cultured in a T-cell growth media (TCGM) consisting of X-VIVO15 (Lonza) supplemented with 5% human serum ...
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bioRxiv - Immunology 2020Quote: ... Activated cells were transfected using the Human T cell Nucleofector Kit (Amaxa Biosystem, #VPA-1002) and the Amaxa Nucleofector II system (Lonza), Program T-023 with ribonucleoprotein complexes ...
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bioRxiv - Immunology 2020Quote: ... Transfection of PBMCs: ASOs were electroporated into PBMCs using the Human T cell nucleofector kit (VPA-1002, Lonza, Basel, Switzerland). Transfection of TILs ...
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bioRxiv - Immunology 2021Quote: ... the T cell / RNP / HDRT mixture was transferred onto a 16-well electroporation strip (20 µl = 106 cells per well, Lonza) or an electroporation cartridge (100 µl = 5×106 cells ...
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bioRxiv - Molecular Biology 2022Quote: ... D-PBS was gently aspirated from the T cell pellet and then resuspended in 15 μL of buffer P3 (Lonza). The cell suspension was then transferred to the RNP mix and thoroughly triturated ...
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bioRxiv - Microbiology 2022Quote: ... transfection was performed with 3.5 μg of PF16eYFPNeo introduced into 108 cells using a Human T-cell kit and IIb Nucleofector set to program X-001 (Lonza). Other transfections described in this work used the same conditions except with Tb-BSF buffer (90 mM sodium phosphate ...
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bioRxiv - Cancer Biology 2022Quote: ... and irradiated allogeneic PBMC (50 Gy) pooled from three donors as feeder cells in T-cell medium RPMI 1640 plus (Lonza):AIM-V (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured in individual wells of a 24-well plate in T-cell media consisting of RPMI 1640 plus (Lonza) supplemented with 10% human AB serum (BST) ...
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bioRxiv - Immunology 2023Quote: ... isolated WT P14 CD8+ T cells were washed with PBS and mixed with RNPs by using P3 Primary Cell 4D-NucleofectorTM X Kit (Lonza) immediately prior to electroporation (Lonza 4D-nucleofactorTM core unit ...
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bioRxiv - Biophysics 2023Quote: ... the ASMC_1 and ASMC_2 cells were transferred into a T-75 flask for an entire week in the growth medium (SmGM-2, Lonza). The cells were incubated at 37°C and 5% CO2 to maintain the pH at 7.2-7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... HMLE cells were generated by introducing SV40 large T-antigen and human telomerase reverse transcriptase into a primary culture of normal mammary epithelial cells (Lonza). HMLE cells were cultured in MEGM ...
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bioRxiv - Immunology 2023Quote: ... Transfections were performed with an Amaxa Nucleofector device: 2×106 cells were resuspended in 100 μL Nucleofector solution T (Lonza) and added to 2 µg of pcDNA3.1 expression vector encoding full-length IL-2Rβ ...
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bioRxiv - Bioengineering 2023Quote: ... iPSC and T cell electroporation were performed using the Amaxa™ 96-well Shuttle™ with the 4D Nucleofector (Lonza). Cas9 nucleases and sgRNAs were precomplexed in supplemented Nucleofector® Solution for 20 min at room temperature and the RNP solution was made up to a final volume of 2.5 µL (10X) ...
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bioRxiv - Microbiology 2023Quote: ... amazonensis promastigotes using the Human T-Cell Nucleofector kit and the Amaxa Nucleofector electroporator (program U-033, Lonza, Basel, Switzerland), for integration into the 18S rRNA locus within the nuclear DNA ...
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bioRxiv - Cancer Biology 2022Quote: ... An iCasp9-CD20/CD19-CAR transposon minicircle carrying CD20 scFv[17] and CD19 scFv[18] and a qPBase plasmid were delivered into activated T cells using 4D-Nucleofector device (Lonza) and Quantum Nufect Kit (GenomeFrontier Therapeutics ...
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bioRxiv - Bioengineering 2022Quote: ... B and T cell electroporations were performed using the Amaxa™ 96-well Shuttle™ with the 4D Nucleofector (Lonza). HSPC electroporations were performed in MaxCyte ATx ...
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bioRxiv - Immunology 2023Quote: CD4+T cells were cultured immediately after isolation in 24-well plates (106 cells/well) containing RPMI 1640 medium (Lonza) with 10% FCS ...
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bioRxiv - Cancer Biology 2024Quote: ... resuspended in 20 μL of P3 nucleofection/solution plus cas9/sgRNA mixture (P3 Primary Cell 4D-NucleofectorTM X Kit S) for primary T cells or SF Cell Line 4D-NucleofectorTM X Kit S (Lonza) for MM cell lines ...
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bioRxiv - Synthetic Biology 2024Quote: ... negative selection of T cells was performed using the EasySep human T cell isolation kit (Stemcell) and cultured in X-VIVO 15 medium (Lonza) supplemented with 5% fetal bovine serum (FBS) ...