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Citations for Lonza :
3701 - 3750 of 4689 citations for Dengue Virus Serotype 1 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... HMLER Snail-ER and HLMER-Snail (a gift from Robert Weinberg) cells were grown in 50% MEGM (Lonza) and 50% DMEM/F12 50:50 (Corning ...
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bioRxiv - Immunology 2021Quote: ... The virus was propagated in the human neuroblastoma cell line SK-N-SH in Eagle’s Minimum Essential Medium (EMEM) with Earle’s Balanced Salt Solution (EBSS) (Lonza), supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2020Quote: ... using the Cell Line Nucelofector Kit V and the Amaxa Nucleofector II Device (Lonza Group AG, Basel, CH). Stable cells were selected for with 1mg/mL hygromycin B (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: A human breast MCF10A cell line (CRL10317, ATCC) was grown with completed growth medium: MEGM Kit (Lonza CC3150) without gentamycin-amphotericin B mix (GA1000 ...
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bioRxiv - Cell Biology 2020Quote: ... EB3-mCherry construct (Stepanova et al., 2003) was transiently transfected using Amaxa Cell Line Nucleofector kit V (Lonza), program X-001.
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bioRxiv - Cell Biology 2020Quote: Jurkat T cells (clone E6.1; ATCC TIB-152) were grown in RPMI 1640 medium w/ L-Glutamine (Lonza) supplemented with 10% Fetal Bovine Serum and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... Both cell lines were regularly tested for mycoplasma contamination by MycoAlert PLUS Mycoplasma Detection Kit (Lonza, LT07-703). REF52 cells were transiently transfected with mEos3.2-Actin expression vector (Michael W ...
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bioRxiv - Bioengineering 2020Quote: Human bone marrow-derived mesenchymal stromal cells (MSCs) from a single donor were purchased from Lonza (Walkersville, MD). The trilineage potential of the cells was confirmed through induction in lineage-specific media ...
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bioRxiv - Microbiology 2021Quote: ... The human lung epithelial cell line Calu-3 (ATCC HTB-55) was maintained in DMEM F-12 (Lonza) supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Vero E6 cells were obtained from the ATCC (ATCC CRL-1586) and cultured in DMEM (Lonza, Basel, Switzerland) supplemented with 5% (FBS (EuroClone ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... the siRNAs were transfected into human CD4+ T cells using the Amaxa Nucleofector following the manufacturer’s protocols (Lonza). The siRNAs used in this study were purchased from GenePharma and the sequences are as below:
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bioRxiv - Molecular Biology 2020Quote: HEK293 cells were passaged in poly-D-Lysine treated plates and incubated overnight in OPTI-MEM medium (Lonza) at 37°C ...
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bioRxiv - Immunology 2022Quote: ... All cell lines were confirmed to be free of mycoplasmas using the MycoAlert detection kit (Lonza, Basel, Switzerland). Cells were detached in PBS EDTA 5mM without enzymatic solution to avoid HVEM cleavage.
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bioRxiv - Physiology 2022Quote: ... Media is removed and the cell pellet is resuspended in 3mL of ACK lysis buffer (Lonza 10-548E) for 2 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... All cells were negative for Mycoplasma upon regular testing with the MycoAlert Mycoplasma Detection Kit (Lonza; LT07-318). LARP1 knockout cell line generation was performed using Edit-R™ CRISPR-Cas9 Gene Engineering ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were routinely tested for mycoplasma contamination using MycoAlert PLUS mycoplasma detection kit (Lonza, MD, USA) and molecularly characterised using an “in house” panel of cellular and molecular markers to check that cell lines have not been cross contaminated (every 3-6 month) ...
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bioRxiv - Biochemistry 2022Quote: ... Cell lines were regularly tested and verified to be mycoplasma negative using MycoAlert PLUS Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were regularly tested for mycoplasma contamination using a biochemical test kit (#LT07-318, Lonza, Switzerland) and were free of mycoplasma contamination.
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bioRxiv - Cell Biology 2022Quote: ... 25 μg mRNA was transfected into 2.5 × 106 freshly thawed fibroblasts using Cell Line Nucleofector Kit V (Lonza) and AMAXA program X-001 following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Transient transfections of these dominant-negative Rab11 a and siRab11 a into mouse MΦs (RAW 264.7 cell line) were performed with Amaxa mouse macrophage nucleofector kit (VPA-1009, Lonza) and DharmaFECT 4 Transfection Reagent (T-2004-01 ...
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bioRxiv - Cell Biology 2022Quote: ... Cell cultures were washed with Dulbecco’s phosphate buffered saline without calcium or magnesium (DPBS-; Lonza, Walkersville, MD, USA) and detached with 0.25% trypsin with 0.53 mM EDTA (ATCC ...
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bioRxiv - Cell Biology 2022Quote: ... expression constructs were transfected into cells using the Amaxa™ 4D-Nucleofector (10μg plasmid cDNA; Lonza, Basel, Switzerland) or JetPrime (2μg plasmid cDNA ...
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bioRxiv - Cell Biology 2022Quote: ... All cell lines were routinely tested for mycoplasma contamination by using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza).
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bioRxiv - Microbiology 2022Quote: ... Both HeLa M and 293T cells and their derivatives were maintained in Dulbecco’s modified Eagle medium (DMEM) (Lonza) supplemented with 10% of fetal calf serum (FBS ...
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bioRxiv - Systems Biology 2022Quote: Human Dermal blood microvascular endothelial cells (DMECs) isolated from a single donor were purchased from Lonza (CC-2183). DMECs were cultured in EGM2-MV (Lonza ...
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bioRxiv - Molecular Biology 2024Quote: ... siRNA transfections were performed using the 4D-Nucleofector and the SE cell line 4D-Nucleofector X kits (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... were added to ABCs and EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ (Lonza, CC-3162) with iMatrix was added to HUVECs and then incubated in 37°C at 5% O2 ...
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bioRxiv - Molecular Biology 2024Quote: ... iMEPM cells were tested for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza Group Ltd, Basel, Switzerland). Packaged lentiviruses containing pLV[shRNA]-EGFP:T2A:Puro-U6>Scramble_shRNA (vectorID ...
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bioRxiv - Synthetic Biology 2024Quote: ... and Jurkat cell lines tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza Cat #LT07-318).
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bioRxiv - Neuroscience 2024Quote: ... we used the 4D-Nucleofector system and Amaxa P3 primary Cell 4D-Nucleofector X Kit S from Lonza. The electroporation buffer used was P3 primary cell Nucleofector Solution with Supplement 1 (Lonza) ...
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bioRxiv - Immunology 2024Quote: ... All recovered cell fractions were rested overnight in EP-TICLE culture media consisting of X Vivo-15 (Lonza) supplemented with 2% KnockOut serum replacement (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... All cell lines used were confirmed to be negative for mycoplasma by annual testing using MycoAlert Plus (Lonza). Last testing was performed on 14 August 2019.
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bioRxiv - Microbiology 2024Quote: ... Cell lines were confirmed to be mycoplasma-negative in a MycoAlert PLUS assay (Lonza catalog number LT07-710) performed by the Koch Institute High-Throughput Sciences Facility (Cambridge ...
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bioRxiv - Immunology 2024Quote: ... 16.4µl P3 and 3.6µl Supplement Reaction solutions from P3 Primary Cell 4D X Kit S (Lonza V4XP-3032) were added ...
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bioRxiv - Bioengineering 2023Quote: ... were cultured using the EGM™-2 MV Microvascular Endothelial Cell Growth Medium BulletKit™ (Lonza, CC-3202), which contains hydrocortisone ...
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bioRxiv - Cell Biology 2023Quote: Human mammary epithelial cells (HMECs) were cultured at 37°C with 5% CO2 in MEBM basal medium (Lonza) supplemented with MEGM SingleQuots (Lonza ...
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bioRxiv - Genetics 2023Quote: ... 2−106 lymphoblastoid cells were suspended in 100 μL of Nucleofector C solution (VCA-1004, Lonza Cologne AG) with 0.6 μM RNAi and transfected with the Z-001 program according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were loaded in these wells in human T cell media (X-VIVO media, Lonza, Cat# 04-418Q) supplemented with 5% human AB serum ...
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bioRxiv - Cell Biology 2023Quote: ... All human cell lines were tested for Mycoplasma contamination using a MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland).
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines were periodically tested to confirm Mycoplasma negativity using the MycoAlert mycoplasma Detection Kit (Lonza, Visp, Switzerland). Detailed methods for additional cell lines derived from solid tumors and leukemia are provided in Supplementary Materials and Methods.
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bioRxiv - Immunology 2023Quote: Electroporation was performed to introduce siRNA into BMDCs using a mouse dendritic cell nucleofector kit (Lonza, Basel, Switzerland) and a Nucleofector 2b (Lonza) ...
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bioRxiv - Microbiology 2023Quote: ... Parasites and host cells were regularly tested for Mycoplasma contamination using the Mycoalert detection kit (Lonza; Basel, Switzerland). For all assays ...
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bioRxiv - Molecular Biology 2023Quote: ... All cell lines were frequently tested for mycoplasma using MycoAlert Plus Mycoplasma Detection Kit (cat# LT07-218, Lonza).
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An indocyanine green-based liquid biopsy test for circulating tumor cells for pediatric liver cancerbioRxiv - Cancer Biology 2023Quote: ... cells were kept in culture for 96 hours in complete hepatocyte basal medium (cat. no. CC-3198, Lonza). Cells were then fixed in 4% paraformaldehyde (cat ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were tested negative for mycoplasma contamination with MycoAlert® Mycoplasma Detection Kit (Lonza, LT07-118).
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bioRxiv - Genetics 2023Quote: ... The cell suspension was transferred to the nucleofection cuvete and nucleofection was performed on the 4D-Nucleofector (Lonza) on the program for hESC ...
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bioRxiv - Cell Biology 2023Quote: ... Two days after transfection of WT HeLa cells with pSpCas9(BB)-2A-GFP-ghMyo10 using Amaxa nucleofection (Lonza), GFP-positive cells were subjected to single-cell sorting into 96-well plates using a BD FACS cell sorter ...
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bioRxiv - Genomics 2023Quote: 2 × 105 HCT116-Cas9 and iPSC-iCas9 were resuspended in 20 µl SE cell line nucleofection solution (Lonza) (HCT116-Cas9 ...
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bioRxiv - Molecular Biology 2023Quote: ... or Vero-hACE2-TMPRSS2 cells (Vero AT) (BEI NR-54970) in Eagle’s Minimum Essential Medium (Lonza, 12-125Q) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼2-4 million cells were electroporated using the T-020 method of the Nucleofector™ 2b device (Lonza). Immediately after electroporation ...