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Citations for Lonza :
2551 - 2600 of 2664 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The media was removed from the cell pellet and cells were resuspended according to the protocol provided for the P3 Primary Cell 4D-Nucleofector Kit (Lonza, V4XP-3024). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lines were cultured in a humidified incubator at 37°C and 5% CO2 and regularly tested for mycoplasma using the MycoAlert mycoplasma detection kit (Lonza, LT07-418).
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were authenticated by STR profiling (Eurofins, Val Fleuri, Luxembourg) and were tested negative for mycoplasma (MycoAlert PLUS mycoplasma detection kit, Lonza, Basel, Switzerland).
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bioRxiv - Immunology 2023Quote: ... Constructs were transiently transfected into KRT17 null A431 cells using the SF Cell Line 4D-Nucleofector™ X Kit (Lonza #V4XC-2032) and Lonza 4D-nucleofector X unit “A431 cell” program ...
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bioRxiv - Cancer Biology 2024Quote: ... Mycoplasma testing was performed on all cells when they were thawed and semi-regularly thereafter using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza LT07-703). Independent experiments were performed on cells treated with siRNA and/or compounds from separate passages of each cell line ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lines were authenticated by short tandem repeat analysis and routinely tested for mycoplasma using the MycoAlert mycoplasma detection kit (Lonza, #LT07-710).
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bioRxiv - Cancer Biology 2024Quote: ... after thaw for about 1 week and tested for mycoplasma regularly using the MycoAlertTM PLUS Mycoplasma Detection Kit (Lonza, CAT#: LT07-318). Cells were always used within 10 passages of authentication via STR analysis with CellCheck9 by IDEXX laboratories.
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bioRxiv - Genetics 2024Quote: ... 3x105 Daudi cells were nucleofected with 20 picomole of sgRNA-Cas9 complex and 100 picomole of DNA donor template using program CA137 of Amaxa 4D-Nucleofector and SF cell line kit S (Lonza, V4XC-2032). The edited single-cell clones were sorted into 96-well plate by BD Aria II sorter and expanded for 4 weeks ...
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bioRxiv - Cell Biology 2024Quote: ... was transfected into 106 U2OS cells using the Amaxa Nucleofector System with the Cell Line Nucleofector Kit V (catalog no: VCA-1003, Lonza, Cologne, Germany) utilizing program X-001 according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... and 1.2 μl of Alt-R® Cas9 Electroporation enhancer (100 μM, IDT) using P3 Primary Cell Nucleofector® 4D Kit and Nucleofector 4D system (Lonza). After electroporation ...
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bioRxiv - Systems Biology 2024Quote: ... HiFi Cas9 Nuclease V3 protein (IDT, 1081058) were introduced into low-passage dual-reporter ESCs using the Mouse Embyronic Stem Cell Nucleofector Kit (Lonza VPH-1001). ESCs were treated with 0.025% trypsin/1% EDTA (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were authenticated and routinely tested to be mycoplasma-free using the MycoAlert Mycoplasma Detection Kit (Lonza, Cat # LT07-710) every two months ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were maintained at 37°C and 5% CO2 in 2D monolayer culture and confirmed as Mycoplasma negative (MycoAlert Mycoplasma Detection Kit, Lonza #LT07-701). Cells were maintained in DMEM medium (Corning #10-013-CV ...
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bioRxiv - Neuroscience 2024Quote: ... following a previously described protocol.43-44 Cultures underwent testing every other month using the MycoAlert Mycoplasma Detection Kit (Lonza LT07-318) and consistently tested negative for mycoplasma throughout the study.
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bioRxiv - Cell Biology 2024Quote: ... the Ribonucleoprotein (RNP) complexes (9:1 sgRNA to Cas9 ratio) were assembled in Nucleofector Solution plus Supplement (Lonza Amaxa HMEC Nucleofector Kit) to a total volume of 100µL ...
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bioRxiv - Cell Biology 2024Quote: ... In microscopy experiments we used the same media but without phenol red to reduce background fluorescence (Lonza CC-3153 phenol-red free basal media supplemented with growth factors and other components from the Lonza CC4136 kit). T98G cells were purchased from ATCC ...
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bioRxiv - Microbiology 2024Quote: ... The resuspended cells were combined with crRNP complexes and were immediately transferred into the cuvette of the P3 Primary Cell Nucleofector Kit (Lonza; V4SP-3096) and electroporated using code CM-137 on the Lonza 4D-Nucleofector ...
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bioRxiv - Microbiology 2024Quote: ... Cells were treated with mycoplasma removal agent (MP Biomedical, #3050044) and tested monthly for mycoplasma contamination using the MycoAlert Plus mycoplasma testing kit (Lonza, #LT07-318).
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bioRxiv - Microbiology 2024Quote: ... with an overnight digestion prior to phenol-chloroform purification and transfection with the P3 Primary Cell 4D-Nucleofector kit (Lonza V4XP-3032) in a Amaxa 4D Nucleofector (Lonza AAF-1003X ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lines were authenticated using short tandem repeat (STR) analysis and tested negative for mycoplasma using the MycoAlert mycoplasma detection kit (Lonza, Basel, Switzerland). Sigma Mission shRNAs targeting Maf1 (TRCN0000182935 ...
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bioRxiv - Neuroscience 2024Quote: ... RNPs were then nucleofected into 1.2-5X106 monocytes isolated the prior day using the P3 Primary Cell 4D-Nucleofector® LV Kit (Lonza #V4LP-3520) on program CM137 ...
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bioRxiv - Immunology 2024Quote: CYLD was knocked down by the CRISPR/Cas9 method using SG cell line 4D-nucleofector X kit (#V4XP-4024, Lonza, Basel, Switzerland). For 1x106 cells ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 3 × 106 cells were centrifuged and nucleofected using the P3 Primary Cell 4D-Nu-cleofector X Kit L (Lonza, V4XP-3024), a 4D-nucleofector core unit ...
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bioRxiv - Physiology 2024Quote: ... These cells were cultured in complete endothelial cell growth medium-2 (EGM-2) supplemented with the Bullet Kit (Lonza Biologics, Basel, Switzerland) and a 1% v/v penicillin-streptomycin antibiotics cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... All cell lines were authenticated through ATCC STR profiling and tested regularly for mycoplasma using the Lonza MycoAlert detection kit (Lonza LT07-318).
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bioRxiv - Microbiology 2024Quote: ... The resuspended cells together with RNP complexes were immediately transferred into the cuvette of the P2 Primary Cell Nucleofector Kit (Lonza; V4SP-2096) and electroporated using the program code ...
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bioRxiv - Cancer Biology 2024Quote: ... which was introduced into CMK cells using a 4D nucleofector system and P3 primary cell 4D-Nucleotransfector X Kit L (Lonza; Basel, Switzerland). Post transfection cells were cultured in 12-well plates ...
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bioRxiv - Immunology 2024Quote: ... Cell cultures were regularly analyzed for mycoplasma contamination using the MycoAlert™ Mycoplasma detection Kit following the manufacturer’s protocol (Lonza, #LT07-318) and were always tested negative for mycoplasma infections
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bioRxiv - Bioengineering 2024Quote: CHO cells were transiently transfected by electroporation using the Amaxa Nucleofector system in combination with the SG Cell Line 96-well Nucleofector® Kit (Lonza, Switzerland). 1.86 x 106 cells per well were transfected with 750 ng plasmid DNA or 400 ng mRNA following manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... All the cells were grown with 5% CO2 at 37°C and verified mycoplasma-free using the MycoAlert Mycoplasma Detection Kit (Lonza, LT07-218). The differentiation of WTC11 i3N iPSCs into excitatory neurons was performed using a two-step differentiation protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 20uL prepared electroporation buffer (16.4uL SE or P3 + 3.6uL supplement buffer per reaction) provided by the Lonza 4D-Nucleofector X kit (SE, Fisher Scientific, #NC0828118; or P3, Lonza; #V4XP-3032.) 20uL of the cell solution was then transferred to the 5uL RNP solution and gently mixed by pipetting ...
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bioRxiv - Genomics 2024Quote: ... 200,000 cells were nucleofected with either 500 ng pJK028A and 500 ng pJK029A or 1 μg pJKEB01 using the Lonza SF Cell Line 4D-Nucleofector X Kit S (Lonza, V4XC-2032) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Approximately 12 million cells per replicate were spun down and resuspended in nucleofection solution from the Lonza SF Cell Line 4D-Nucleofector X Kit L (Lonza, V4XC-2012) and nucleofected in 12 reactions with 5 μg pLJK07 or pLJK08 per 1 million cells according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... All iPSC-derived cell lines were tested and negative for mycoplasma contamination using the MycoAlert Mycoplasma test kit from Lonza (#LT07-318).
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bioRxiv - Immunology 2024Quote: ... Human primary fetal tissue was only used after it was confirmed negative for mycoplasma contamination using the MycoAlert Mycoplasma test kit from Lonza (#LT07-318).
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bioRxiv - Bioengineering 2024Quote: Sequence confirmed validation plasmids were nucleofected into the PB Tet-On dCas9 K562 cell lines using SF Cell Line 96-well Nucleofector kit (Lonza, V4SC-2096). The manufacturer’s instructions were followed using 800 ng plasmid DNA per nucleofection ...
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bioRxiv - Bioengineering 2024Quote: CHO-S cells were stably transfected by electroporation using the Amaxa Nucleofector system in combination with the Cell Line Nucleofector® Kit V (Lonza, Switzerland). 5.0 x 106 cells per cuvette were transfected with 2 µg plasmid and incubated in T75 flasks at 37 °C under 5% CO2 in 13 ml CD-CHO medium supplemented with 8 mM L-glutamine ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lines were authenticated by short tandem repeat DNA profiling (Genetica DNA laboratories) and tested for mycoplasma contamination routinely using the MycoAlert PLUS detection kit (Lonza #LT07–710) and Mycoplasma detection kit from Southern Biotech ...
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bioRxiv - Cell Biology 2024Quote: ... and immediately transfected by Nucleofection into MCF-10A or HaCaT cells using the Amaxa SF Cell Line 4D-Nucleofector X kit S (Lonza, PBC2-00675) and the program DS-138 (MCF-10A and HaCaT ...
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bioRxiv - Bioengineering 2024Quote: ... 1,000,000 NIH/3T3 cells were centrifuged (200× g, 3 minutes) and resuspended in 100 µl of the SG Cell Line nucleofection Kit (Lonza, V4XC-3024). After the addition of 500 ng plasmid DNA encoding the hyperactive PiggyBac transposase and 1000 ng of the NTVE knock-in plasmid ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lines were routinely tested and shown to be negative for Mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza, Cat# LT07-118). Cells were passaged by detachment with 1 x Trypsin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... K562 cells were nucleofected with 600 ng of pMH224 or pMH260 and 200 ng of a separate PiggyBac transposase plasmid using SF Cell Line 96-well Nucleofector kit and protocol (Lonza, V4SC-2096) (Supplementary Table 8 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 × 105 OVCAR8 landing pad cells were resuspended in 20 µl of nucleofector solution combined with supplement at 4.5:1 from the Amaxa SE Cell Line 4D-Nucleofector X Kit S (Lonza, Cat #V4XC-1032), before addition of 5 µl RNP complex and 1 µl of Electroporation enhancer ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 × 106 cells were electroporated with 2 μg of each plasmid using program A23 of Nucleofector II (Amaxa) and mouse stem cell Nucleofector kit (Lonza DPH-1001) or human stem cell Nucleofector I kit (Lonza VPH-5012) ...
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bioRxiv - Cell Biology 2024Quote: RAW 264.7 cells and MDA-MB-231 cells were transitly transfected by nucleofection in an Amaxa Nucleofector II device using Amaxa Cell line kit V (Lonza, VACA-1003) following a pre-existing protocol [101] ...
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bioRxiv - Cancer Biology 2021Quote: ... All cell lines used in this study tested negative for Mycoplasma contamination via MycoAlert™ PLUS Mycoplasma Detection Kit (LT07-710, Lonza, Basel, Switzerland).
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bioRxiv - Genomics 2020Quote: ... 2*106 TX1072 mESCs were electroporated with 2µg of each guide plasmid and 30pmol of the single stranded repair oligo using the P3 Primary Cell 4D-Nucleofector X Kit (V4XP-3024) with the Amaxa 4D Nucleofector system (Lonza, program CP-106) and plated on gelatin-coated 10cm dishes ...
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bioRxiv - Cancer Biology 2020Quote: ... All cells were tested bimonthly – every 2 months – to ensure mycoplasma-free cell culture using MycoAlert™ mycoplasma detection kit (Lonza, #LT07-418) and MycoAlert™ assay control set (#LT07-518) ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were routinely authenticated using short tandem repeat profiling and verified to be free of mycoplasma using the MycoAlert mycoplasma detection kit (Lonza Cat# LT07-418), by the QIMR Berghofer Medical Research Institute analytical facility (Brisbane ...
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bioRxiv - Cancer Biology 2020Quote: ... A total of 1.5×106 tumor cells (td-tomato MDA-MB-231 SORE6>GFP) were transfected with 10 μl of 20 μM siRNA stock solution using Nucleofector Kit V from Lonza (cat# VCA-1003) for 48 h ...