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Citations for Lonza :
201 - 239 of 239 citations for PB Cadherin Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... D-PBS was gently aspirated from the T cell pellet and then resuspended in 15 μL of buffer P3 (Lonza). The cell suspension was then transferred to the RNP mix and thoroughly triturated ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed once in PBS and then resuspended in 1 ml of ACK Red Blood Cell lysis buffer (Lonza). After washing with PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were washed twice with PBS and 2 × 106 cells were resuspended in 100 μl of Solution V (Lonza) and then mixed with 5 μg purified sgRNA expression vector or the empty vector as a negative control before nucleofection using program Q-001 of a Nucleofector™ 2b (Lonza) ...
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bioRxiv - Immunology 2023Quote: ... isolated WT P14 CD8+ T cells were washed with PBS and mixed with RNPs by using P3 Primary Cell 4D-NucleofectorTM X Kit (Lonza) immediately prior to electroporation (Lonza 4D-nucleofactorTM core unit ...
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bioRxiv - Cell Biology 2023Quote: ... T cells were washed with PBS and resuspended at 100x106 cells/mL in Lonza P3 Primary Cell 4D Nucleofector Kit buffer (Lonza). 1μg/1x106 cells of sgRNA (Agilent ...
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bioRxiv - Microbiology 2023Quote: Primary monocytes (3 × 106 cells/transfection) were washed with 1X PBS and resuspended in 100 μl of P3 Primary Cell Nucleofector solution (Lonza) containing either 1000 ng empty vector (EV ...
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bioRxiv - Immunology 2023Quote: Cells were then washed with PBS and resuspended in 20 µL of SF (K562) or SG (Raji and Ramos) buffer (Lonza) and mixed with Cas9 RNPs ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5-1 μL of a solution containing 1 μg/μL of pCAG-PBase plasmid and 1.5 μg/μl of pPbCag-GCaMP6s plasmid in 1X phosphate buffered saline (PBS; Lonza, Basel) and 0.0025% of the dye Fast Green (Sigma-Aldrich Co. ...
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bioRxiv - Bioengineering 2023Quote: ... washed three times in PBS and cultured on collagen-coated 6-well plates in endothelial growth medium (EGM-2, Lonza) composed of endothelial basal medium supplemented with 2% fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: ... 10 mL of exponential culture was centrifuged (3250 g, 25 min) and the pellet was resuspended in the same volume of PBS (Lonza) with addition of 10 µM CFSE (Sigma ...
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bioRxiv - Immunology 2023Quote: ... A total of 5×106 cells per transgene were washed with phosphate-buffered saline (PBS) and resuspended in 100µl NucleofectorTM Solution V (Lonza, Germany) containing the expression vector (Fig ...
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bioRxiv - Immunology 2023Quote: ... To this end T cells were washed once in PBS and resuspended in complete P3 nucleofection buffer (Lonza, #V4XP-3032). 1e6 cells were then mixed with either Cas9 or base editor mRNA (4 - 4.5 µg ...
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bioRxiv - Genetics 2023Quote: ... 2 day pre-stimulated CD34+ HSPCs were washed in PBS before being resuspended in P3 nucleofector solution (Lonza, V4XP-3032). An equivalent of 2.5×105 CD34+ HSPCs were mixed with gRNA:Cas9 complexes and 3 µg ssDNA donor template (20 μL ...
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bioRxiv - Cancer Biology 2024Quote: ... by flushing one femur with PBS 2% FBS followed by erythrocyte elimination with ACK Lysing Buffer (10-548E, BioWhittaker, Lonza) (Figure 4BC) ...
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bioRxiv - Neuroscience 2021Quote: ... was pressure-injected from a single barrel glass micropipette into the brain tissue or dilute agarose gel (0.3% w/v in PBS; NuSieve GTG agarose, Lonza, Cat. # 50081) and a sequence of images were taken using a charged-coupled device (CCD ...
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bioRxiv - Microbiology 2019Quote: ... the antibody-virus mixture was aspirated and Vero cells were washed with PBS and overlaid with DMEM containing 2% heat-inactivated FBS and 1% SeaPlaque Agarose (Lonza, 50501). After 4–6 days ...
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bioRxiv - Bioengineering 2022Quote: ... The latter prints were post-treated by soaking in sterile 1x phosphate-buffered saline without calcium and magnesium (PBS, Prod. No. 17-516F, Lonza, USA) for 24 hours at 37°C for BV007a or at 50°C (BioMed and Clear resins ...
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bioRxiv - Immunology 2022Quote: ... in 100 μl PBS (GE Lifesciences, Marlborough, MA) and were transfected by electroporation using a 4D-Nucleofector System (Lonza, Walkersville, MD) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Buffy coat cells were washed three times in 2.5 mM EDTA-PBS at 4°C before dilution and maintenance in RPMI-1640 supplemented with 10% FCS (PAA, AT or Lonza, CH) 2 mM glutamine ...
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bioRxiv - Physiology 2022Quote: ... 1 million cells were washed with PBS and electroporated with 5-10 μg of appropriate plasmid using Amaxa cell nucleofector kit T (Lonza laboratories). Cells were allowed to recover for 48 h ...
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bioRxiv - Microbiology 2022Quote: ... Sections attached to slides were fixed with cold acetone/ethanol (1:1) solution and washed in PBS buffer (Lonza, Morristown, NJ). Primary antibodies and isotype controls (Supplementary Table 9 ...
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bioRxiv - Physiology 2022Quote: ... (5 g/L trypsin and 2 g/L EDTA diluted 1 in 5 in calcium- and magnesium-free PBS) (Lonza, UK).
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bioRxiv - Physiology 2022Quote: ... 5 million cells were washed with phosphate buffered saline (PBS) and electroporated with 4-6 μg of appropriate plasmid construct using an Amaxa cell nucleofector (Lonza laboratories) and nucleofection reagent (362.88 mM ATP-disodium salt ...
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bioRxiv - Microbiology 2023Quote: ... 2 ×□106 unstimulated CD4+ T cells or 0.2 × 106 CD34+ cells were washed with PBS and resuspended in 20□μl buffer P3 (Lonza #V4XP-3032). To deliver two specific sgRNAs targeting one gene ...
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bioRxiv - Genetics 2023Quote: ... The pellet was washed with 10 ml of 1 x PBS and resuspended in 20 µl of nucleofection SF buffer (Lonza Biosciences), in which 600 ng of the DsRed2-N1 plasmid was added to the suspension ...
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bioRxiv - Bioengineering 2024Quote: ... The day of the electroporation cells were collected and washed twice with phosphate buffered saline (PBS) and resuspended in nucleofection buffer (SF/SE Cell Line 96-well Nucleofector™ Kit; Lonza) containing 2 μg of PEn mRNA and 100 pmol of synthetic springRNA or spaceRNA ...
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bioRxiv - Cancer Biology 2019Quote: ... Animals were sacrificed by cervical dislocation four days after electroporation and resected xenografts were fixed in 4% (w/v) paraformaldehyde (PFA) in phosphate buffered saline (PBS) (Lonza, Basel, Switzerland) at 4°C for approximately one week ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.1% SDS) supplemented with protease inhibitors (MilliporeSigma, 11836170001) was added to each well after rinsing with PBS (Phosphate Buffered Saline; Lonza, 17-517Q). Cells were scraped into RIPA buffer on ice using 1 mL pipette tips ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were washed 1x in RT PBS and transfected with ∼15μg of DNA plasmid using Amaxa nucleofector 2b (Lonza, program O-005). After transfection ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels were fabricated using a solution consisting of lyophilized GelMA (5 wt%) dissolved at 37°C in phosphate buffered saline (PBS; Lonza 17-516F) and combined with 0.1% w/v lithium acylphosphinate (LAP ...
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bioRxiv - Immunology 2022Quote: 1 × 105 HLFs were mixed with 2 μg of 100 μg/ml of the above expression plasmids in 100 μl PBS (GE Lifesciences, Marlborough, MA) and were transfected by electroporation using a 4D-Nucleofector System (Lonza, Walkersville, MD) following the manufacturer’s protocol CZ-167 ...
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bioRxiv - Microbiology 2023Quote: Colons from infected mice were open longitudinally and gently washed with ice-cold 1X PBS before being placed in Hanks’ balanced salt solution (HBSS) with phenol red and without calcium and magnesium (Lonza, Basel, Switzerland) on ice ...
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bioRxiv - Genomics 2023Quote: ... were washed twice with PBS and resuspended in a solution containing a 4.5:5 mixture of nucleofection buffer (Buffer SE, Lonza Lot #: S-09279) and supplement (Lonza ...
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bioRxiv - Cell Biology 2021Quote: ... endotoxin-free phosphate buffered saline (PBS) and verified to be free of detectable lipopolysaccharide contamination by Limulus lysate assay (Lonza, Walkersville, Maryland, USA), MCSF (10 ng/mL ...
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bioRxiv - Cell Biology 2020Quote: ... Conditioned media (containing EVs) was removed and the cells washed x 2 with PBS before trypsinisation using 1 × Trypsin-EDTA (Lonza, UK cat: T3924). Cell counts and viability were checked at the time of EV harvest using the trypan blue exclusion assay (0.4% Trypan blue solution ...
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bioRxiv - Immunology 2024Quote: ... including the use of 2 mM EDTA in PBS as the wash buffer and red blood cell lysis with ACK lysis buffer (Lonza by Thermo Fisher). Subsequently ...
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bioRxiv - Synthetic Biology 2023Quote: ... CD22-deleted clones modified to express PD-L1 (LZRS PD-L1) or CD200 (PB-EF-1α-intron CD200, pCMV-hyPBase, SF buffer kit V4XC-2032, Lonza 4D Nucleofector, code DN100). Transposition reactions used 1-2 μg of vector DNA and 0.5-1.0 μg of pCMV-hyPBase transposase DNA ...
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bioRxiv - Cell Biology 2023Quote: ... to aid complete removal of PBS before resuspension in P3 Nucleofection Solution (room temperature; P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza; cat. no., V4XP-3032). A total of 20 μl solution was used to resuspend 80,000 cells ...
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bioRxiv - Microbiology 2022Quote: ... The cells were finally washed and resuspended in PBS (1 × 106 cells per ml) and molten 1% InCert agarose (Lonza Rockland, Inc., Rockland, ME, USA) in PBS (1:1 vol/vol) ...