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Citations for Lonza :
2251 - 2300 of 2715 citations for Rat Vang Like Protein 2 VANGL2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... All cells used in this study were cultured in a humidified incubator at 37°C with 5% CO2 and routinely tested and certified as mycoplasma-free using the MycoAlert kit (Lonza). STR analysis (Idexx ...
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bioRxiv - Immunology 2021Quote: ... MICB or ULBP-1 genes after optimizing nucleofection conditions using primary cell 4D nucleofector kit and 4D nucleofector system (Lonza). After 48 hours of culture ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were typically maintained in culture for 1-2 months before thawing of a new batch and mycoplasma screening was performed routinely using the MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cancer Biology 2020Quote: ... All cells were confirmed to be mycoplasma-free by MycoAlert Mycoplasma Detection Kit (Lonza, Morristown, NJ USA; Cat #: LT07-118). Cells were steroid-starved in phenol red-free medium containing 10% charcoal stripped-FBS (5% CS-FBS for C4-2 cells ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines were maintained and passaged according to ATCC recommended procedures and regularly tested for mycoplasma using MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cancer Biology 2020Quote: ... All cells were grown at 37°C in a humidified atmosphere with 5% CO2 and tested for lack of mycoplasma contamination (MycoAlert Mycoplasma Detection Kit, Lonza) prior to experiments (initiated within the initial 1-4 passages).
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bioRxiv - Cancer Biology 2021Quote: ... Both cell lines were authenticated by amplification and Sanger sequencing of the BRCA1 locus harboring the original and secondary mutations and regularly tested for Mycoplasma contamination using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza). The two cell lines were maintained in Ham’s F-12 Nutrient Mixture medium with 5% (vol/vol ...
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bioRxiv - Cell Biology 2022Quote: ... were nucleofected with either PGK-mO-Dam or PGK-mO-Dam-Zfp266 plasmids using the P4 Primary Cell 4D-Nucleofector X Kit (Lonza). 5 replicates were performed in total ...
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bioRxiv - Cancer Biology 2022Quote: Cell lines were cultured according to standard aseptic mammalian tissue culture protocols in 5% CO2 at 37 °C with regular testing for mycoplasma contamination using the MycoAlertTM Mycoplasma Detection Kit (Lonza). HCT116 ...
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bioRxiv - Microbiology 2022Quote: ... All cell lines were regularly tested to check they were free of mycoplasma contamination using a commercially available system (MycoAlert Mycoplasma Detection kit; Lonza). HCV replication experiments were conducted using the highly permissive human hepatoma cell line Huh7 High Passage (28 ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 106 NSCs were mixed with 5 µg of corresponding DNA with the Amaxa P4 Primary Cell 4D-Nucleofector X Kit S (Lonza) and pulse was delivered using the CA137 programme in a 4D-Nucleofector X Unit (Lonza) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were transfected with 5μg of selected plasmid (control, empty vector (EV) or containing guide RNAs using Amaxa Cell Line Nucleofector kit (Lonza Bioscience) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 2×105 NSCs for essential gene expression were nucleofected with 750 ng each of the above-mentioned plasmids using SG Cell Line 4DNucleofector X Kit (Lonza), DN-100 program ...
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bioRxiv - Cell Biology 2022Quote: A GFP sgRNA vector was delivered into Cas9 and GFP expressing NSCs using nucleofection with the SG Cell Line 4DNucleofector X Kit (Lonza) as per manufacturer’s instructions51 ...
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bioRxiv - Bioengineering 2022Quote: ... and 1 μg of a plasmid encoding a hyperactive piggyBac transposase (hyPBase) 39 were co-electroporated into Im-HUVEC using the P5 Primary Cell Kit (Lonza) and a 4D-nucleofector (Lonza) ...
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bioRxiv - Microbiology 2022Quote: ... Ribonucleoproteins were introduced into 1 × 106 sub-confluent BlaER1 cells using 4D-Nucleofector X Unit and SF Cell line Kit (Lonza), applying program DN-100 ...
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bioRxiv - Bioengineering 2022Quote: ... All cells were routinely verified as free of mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza Group, Basel, Switzerland). MDA-MB-231 cells were first transduced with lentivirus (MOI=5 ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of eGFP-Centrin1 was achieved by electroporating 4.106 B lymphoma cells with 4 μg of plasmid using the Amaxa Cell Line Nucleofector Kit R (T-016 program, Lonza). Cells were cultured in CLICK medium for 5 to 16 h before imaging.
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bioRxiv - Immunology 2022Quote: ... Twenty μl of cells in buffer were transferred to each well of a 16-well Nucleofection cuvette strip (4D-Nucleofector X kit S; Lonza). Three μl of RNP were gently dispensed to each well ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines in culture were screened biweekly for mycoplasma contamination using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza, #LT07-703).
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bioRxiv - Cell Biology 2022Quote: ... we used Cas9-RNP and the Lonza 4D-Nucleofector System together with the P4 Primary Cell 4D-Nucleofector X Kit L (Lonza) to transfect and create Ndrg2-KO DCs ...
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bioRxiv - Cell Biology 2022Quote: ... For nucleofection we used the 4D-Nucleofector System and P3 Primary Cell 4D Nucleofector X Kit L (Lonza, Basel, Switzerland). We optimized the RNP electroporation for DCs by using multiple-guide mixtures and different number of cells in a 100uL total volume ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were introduced into HCT116 and MDCK cells using nucleofection with the 384-well HT Nucleofector and the SE cell line kit (Lonza) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... All tumor cell lines used in the in vivo experiments were routinely tested for mycoplasma contamination using Mycoalert plus mycoplasma detection kit (Lonza). Randomization of animals was not used in experiments and no blinding was done for the animal experiments.
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bioRxiv - Cancer Biology 2022Quote: ... siControl and siIRAK4 were purchased from Horizon Discovery and transfected into human AML samples using Amaxa Nucleofector Kit T (Lonza) (program number G-016 ...
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bioRxiv - Neuroscience 2021Quote: ... the cell suspension was transfected with a Homer 1c::TdimerDsRed plasmid (Bats et al., 2007) by electroporation using the Amaxa Nucleofector 2b device and Amaxa mouse neuron nucleofector kit (Lonza). Neurons were then plated on poly-L-lysine-coated glass coverslips and grown at 37°C in a humidified 5% CO2-containing atmosphere in BME supplemented with KCl (25 mM final concentration) ...
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bioRxiv - Neuroscience 2020Quote: ... control and C9orf72 iPSNs were nucleofected using the Lonza nucleofection system (program DC104) and a Lonza P3 Primary Cell 4D Nucleofector Kit (Lonza) as described above ...
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bioRxiv - Neuroscience 2021Quote: ... dissociated with Accutase and then transfected with one of the Tau or Tubulin pUCM vectors along with AAVS1 TALEN pairs using a Human Stem Cell Nucleofector Kit 1 (Lonza) with the Nucleofector 2b Device (Lonza) ...
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bioRxiv - Immunology 2022Quote: ... 1*106 purified naïve T-cells were resuspended in 15 μL buffer P3 + 5 μL Supplement 1 (P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza) and mixed with 2.7 μL RNP in the 16-well strips provided in the kit ...
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bioRxiv - Genetics 2022Quote: ... Libraries were electroporated into K562 cells in 100ul volumes using the Amaxa system (Program X-001, Neucleofactor kit V, Lonza). We performed 6 independent replicates with each replicate consisting of ∼2×108 cells ...
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bioRxiv - Immunology 2022Quote: ... 2 million B cells were then washed with PBS and resuspended in 15 uL resuspension buffer (4D-Nuclcofector X Kit S, #V4XP-4032; Lonza). Cells in resuspension buffer (12.5ul ...
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bioRxiv - Immunology 2022Quote: ... Knockdown of Vps4a/b was performed using Amaxa Nucleofector II and the Mouse T-Cell Nucleofector Kit (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... All cells were grown at 37°C and 5% CO2 in a humidified incubator and tested negative for mycoplasma using the MycoAlert Mycoplasma Detection Kit (LT07-318, Lonza).
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bioRxiv - Biochemistry 2022Quote: Generation of overexpressing POLIC variant cell lines was previously described.10 10 μg pLEW79IA-MHTAP was NotI linearized and transfected into 29-13 procyclic cells by electroporation.8 7.5 μg pLew100-PTPPuro plasmids encoding full length POLIB and POLID variants were NotI linearized and transfected into 29-13 cells via Amaxa Nucleofection (Parasite Kit, Lonza). Following selection with 1 μg/mL puromycin for pLew100 based constructs and 2.5 μg/mL phleomycin for cells transfected with pLEW79IA-MHTAP ...
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bioRxiv - Physiology 2022Quote: ... All human iPSC lines were negative for mycoplasma contamination as tested using the Mycoalert Mycoplasma testing kits (LT07-318, Lonza) and no karyotype abnormalities were found (KaryoStat+ ...
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bioRxiv - Developmental Biology 2020Quote: ... Nucleofection was performed on an Amaxa nucleofector IIb using protocol O-003 with the mouse neuron kit (Lonza, VPG-1001). Cells were allowed to recover for 1 hour at 37 °C and followed by NCAM1 MACS and culture as described above.
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bioRxiv - Cancer Biology 2019Quote: ... The cell lines were tested for the absence of mycoplasma using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Allendale, NJ). Cell lines were also authenticated by Genetica DNA Laboratories (Burlington ...
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bioRxiv - Synthetic Biology 2019Quote: U2OS cells stably expressing eGFP.PEST or MDA-MB-231 cells were transfected with Cas9 ribonucleoprotein (RNP) and ssODN using the SE Cell Line 4D-Nucleofector kit (Lonza) following the pulse program of DN-100 (U2OS ...
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bioRxiv - Developmental Biology 2020Quote: ... The guide RNA vector was then electroporated into the parent line containing the CRISPRi system using the Human Stem Cell Nucleofector Kit 1 solution with the Amaxa nucleofector 2b device (Lonza). Nucleofected cells were then seeded into a 6-well plate in mTeSR™-1 supplemented with Y-27632 (10 μM ...
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bioRxiv - Cell Biology 2021Quote: ... was generated by the introduction of a plasmid carrying the EGFP gene under the control of the CAG promoter to EStTA5-4 cells using the Amaxa Mouse ES cells Nucleofector Kit (VPH-1001, Lonza).
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bioRxiv - Microbiology 2021Quote: ... Cells were stimulated by CD3/CD28 Abs for 2 days and nuclofected with 100 μM RORC or non-targeting (NT1) siRNA (ON-TARGETplus SMART pool, Dharmacon) using the Amaxa Human T cell Nucleofector Kit (Amaxa, Lonza), according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2020Quote: ... Cell lines were confirmed to be mycoplasma-negative using the Mycoalert PLUS Mycoplasma detection kit (Lonza, Cat. No.: LT07-703).
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bioRxiv - Microbiology 2021Quote: ... Tightly synchronized schizonts were transfected using the Amaxa 4-D electroporator and P3 Primary Cell 4D Nucleofector X Kit L (Lonza) according to the protocol described by Moon et al ...
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bioRxiv - Immunology 2020Quote: Targeted gene’s expression was knocked-down in up to 5 x 106 cells using the P3 Primary Cell 4D-Nucleofector X Kit L (V4XP-3024, Lonza) with 90 µl P3 Primary cell solution and 100 pmol of corresponding si_RNA (resuspended in 10 ul RNAse-free H2O) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were maintained at 37 °C under 5% CO2 and the cell culture was routinely tested for Mycoplasma using a luminescence-based MycoAlert kit (Lonza).
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bioRxiv - Cancer Biology 2020Quote: ... DRGFP expressing cells were electroporated in reagent T for H520 and H2228 or reagent L (Amaxa Cell Line Nucleofector Kit L, Lonza) for Calu6 with 10ug I-SceI using program X-005 for Calu6 ...
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bioRxiv - Molecular Biology 2021Quote: ... 10,000,000 lymphoblastoid cells were nucleofected with 60 µg of RTW3027 and 20 µg of plasmid expressing gRNA and mCherry using Cell Line NucleofectorTM Kit V (Lonza) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µg RNA was electroporated into BHK-21 cells using an Amaxa nucleofector 2b (program A-031) and Nucleofection T solution kit (Lonza). Transfected BHK-21 cells were mixed in a 1:1 ratio with cells susceptible to CoV infection ...
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bioRxiv - Systems Biology 2020Quote: 5×105 HAP1 cells were transiently transfected with 300 ng of plasmid using the SE cell line 4D-Nucleofector kit S (Lonza), on a 4D nucleofection unit (Lonza ...
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bioRxiv - Genomics 2021Quote: ... were nucleofected with 2 µg of each sgRNA/Cas9 plasmid and 3 pmol or 30 pmol (for E14-STNΔTsixP) repair oligo (Supplementary Table 6) using the Lonza 4D-Nucleofector with the P3 Primary Cell 4D-NucleofectorTM kit (Lonza) and CP-106 (for TXΔXertP and E14-STNΔTsixP ...