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Citations for Lonza :
2251 - 2300 of 2693 citations for Mouse EGF Latrophilin Seven Transmembrane Domain Containing Protein 1 ELTD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Human Umbilical Vein Endothelial Cells (HUVEC) and THP-1 cells were purchased from Lonza Walkersville Inc ...
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bioRxiv - Genomics 2022Quote: ... supplemented with 10% Fetal Bovine Serum (FB12999102, FisherScientific) and 1% Penicillin-Streptomycin (Lonza,17602E). To extract sufficient poly-A RNA ...
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bioRxiv - Microbiology 2024Quote: ... 10 μg of RNA were fractionated on a 2% NuSieve 3:1 agarose (Lonza), 1X MOPS ...
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bioRxiv - Neuroscience 2024Quote: ... The electroporation buffer used was P3 primary cell Nucleofector Solution with Supplement 1 (Lonza). 2.5 × 10 5 phNPCs cells were used per electroporation reaction in one cuvette of the 16-well Nucleocuvette Stripe (Lonza) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 × 106 iPSCs were resuspended in 100 μl of P3 buffer (V4XP-3024, Lonza) containing 20 μg Alt-R® S.p ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg of RNA were fractionated on a 2% NuSieve 3:1 agarose (Lonza), 1X MOPS ...
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bioRxiv - Microbiology 2023Quote: ... with a final concentration of 2% formaldehyde and 2% NuSieve 3:1 agarose (Lonza). 5 µg RNA was denatured for 10 min at 70 °C with 1 X MOPS buffer (20 mM MOPS ...
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bioRxiv - Neuroscience 2023Quote: ... Individual larvae were mounted in a droplet of 1% low melting agarose (50100, Lonza) in the middle of a 60 mm x 15 mm Petri dish and immersed in system water after the agar solidified ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (P/S, Lonza). For both cell types ...
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bioRxiv - Cell Biology 2023Quote: HUVECs were grown on 1% gelatin-coated plates in EGM-2 medium (Lonza: CC3156) for 48 hrs ...
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bioRxiv - Developmental Biology 2023Quote: ... and were separated by electrophoresis in 1-1.2% (w/v) agarose gel (Lonza 50004) supplemented with 0.03μl/ml DNA staining dye (Serva ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 12.5% fetal bovine serum (FBS) and 1% penicillin-streptomycin (P/S, Lonza). Human SH-SY5Y neuroblastoma cells (ATCC ...
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bioRxiv - Molecular Biology 2023Quote: ... Obtained spheroplasts were next embedded into 1 % low melting agarose (InCert Agarose 50123, Lonza) plugs and incubated overnight at 55 °C in a digestion buffer with 1 mg/mL of proteinase K (Euromedex EU0090) ...
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bioRxiv - Biophysics 2021Quote: ... for all imaging experiments involving exogenous expression we used the Lonza Amaxa II Nucleofector System with Cell Line Nucleofector Kit V reagent (Lonza VCA-1003). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Constructs were transfected into 3T3-F442A preadipocytes or OP9 preadipocytes via electroporation using the Amaxa Cell Line Nucleofector Kit V (Lonza, Cologne, Germany). Afterwards cells were grown two days in DMEM ...
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bioRxiv - Developmental Biology 2021Quote: Human isogenic ESCs containing different lengths of CAG repeats in the HTT Exon1 locus (HD-RUES2 6) were nucleofected using the Cell Line Nucelofector II (Kit L from Lonza, Walkersville, MD) by applying the B-016 program ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... Electroporation of K562 cells was performed using a SF Cell Line 4D-Nucleofector kit and 4D-X Nucleofector using pulse code FF-120 (Lonza, Basel, Switzerland), per the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... and CD31+Rspo3+ cells were centrifuged at 500 g for 5 min and resuspended in 500 μl endothelial cell media: EGM-2 MV Bullet Kit (Lonza, cc-3202) supplemented with 50 ng/mL VEGF-C (R&D ...
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bioRxiv - Molecular Biology 2020Quote: ... 4×106 cells were electroporated with 10 μg DNA of pBluescript II SK(+) carrying a human U6 promoter and EGFP-sgRNA using Nucleofector® V Kit (Lonza, Switzerland) as described above.
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: The siRNA knockdown was performed by electroporation using the Amaxa nucleofector I in combination with the Amaxa Cell Line Nucleofector Kit V (Lonza, VCA-1003). SiRNA constructs with the siRNA cloned in the pLKO.1-Puro vector were obtained from the MISSION shRNA library (Sigma product SHGLY) ...
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bioRxiv - Microbiology 2020Quote: ... using the AMAXA Nucleofector 4D system (U-033 setting) and the P3 primary cell 4D-nucleofector X kit with the 16-well nucleocuvette strip (Lonza, V4XP-3032). Clones were obtained by FACS using the FACS Aria II sorter at the Stanford Shared FACS Facility for the brightest red parasites and single cloning the TdTomato+ enriched population by limiting dilution into 96-well plates.
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bioRxiv - Bioengineering 2019Quote: Nucleofection of the HDR template and CRISPR-Cas9 vectors was performed with SF Cell Line 4D-Nucleofection X Kit L (Lonza, V4XC-2024). Before nucleofection hybridoma cells were assessed for viability and centrifuged (90g ...
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bioRxiv - Neuroscience 2019Quote: ... were provided under a MTA and maintained in Clonetics® endothelial cell growth medium-2 MV Bullet Kit (Cat# CC-3162 Lonza, UK) containing the endothelial basal medium ...
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bioRxiv - Neuroscience 2019Quote: ... C57BL/6J: L315 control construct or L315 Nrxn triple knockdown construct) just before plating using AMAXA Nucleofector kit (Lonza, Cat #VPG-1001) and plated at 2500/cm2 on micro islands of mouse (CD-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines identities were validated by STR profiling and tested negative for mycoplasma with MycoAlert Mycoplasma Detection Kit (Lonza, Cat#LT07-418) prior to experimental use.
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bioRxiv - Cell Biology 2020Quote: ... 1×106 cells (grown in 6-well dishes) were subjected to nucleofection with Amaxa® Cell Line Nucleofector® kit V (VCA-1003, Lonza), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... MDA-MB-231 bulk or stable populations overexpressing Nluc-CD63 or Nluc-CD9 constructs were obtained using an electroporation-based transfection protocol optimized for this cell line (Amaxa® Cell Line Nucleofector® Kit V, Lonza). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... subjected to red blood cell lysis with ACK lysis buffer and electroporated with P3 Primary Cell 4D-NucleaofactorTM X -kit (V4XP-3032, Lonza, Basel, Switzerland), as previously described (Freund et al. ...
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bioRxiv - Cell Biology 2021Quote: ... OC activity was measured by releasing the europium conjugated human Type I collagen-coated on the bottom of the OsteoLyse™ Assay Kit (Lonza Biosciences) at each time point ...
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bioRxiv - Cell Biology 2021Quote: ... We transfected the KCNQ1 constructs and the empty vector into our established mouse pancreatic β cells (referred to as SJ β cells (Jia et al., 2015)) using AmaxaTM Cell Line NucleofectorTM Kit V and AmaxaTM NucleofectorTM 2b device (Lonza, Switzerland). The glucose-sensitive mouse β-cell line SJ-β was cultured and an insulin-secretion assay performed as reported earlier (Jia et al. ...
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bioRxiv - Systems Biology 2021Quote: ... freshly isolated neurons (5 million cells per nucleofection reaction) were transfected using the 4D-Nucleofector™ X Unit and the P3 Primary Cell 4D Nucleofector X kit (Lonza, Switzerland), as indicated ...
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bioRxiv - Cell Biology 2020Quote: ... These plasmids were transfected into NHDFs and 7SPs by electroporation using the Amaxa nucleofector kit and nucleofector 2b (Lonza, Walkersvill, MD, USA). Two days later ...
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bioRxiv - Cancer Biology 2021Quote: ... All human cell lines were authenticated by Short Tandem Repeat analysis and tested for Mycoplasma contamination with a MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland).
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bioRxiv - Genomics 2022Quote: ... 8 million cells were transfected with 20ug of plasmid DNA in one 100- uL cuvette using the Amaxa® Cell Line Nucleofector® Kit V (Lonza), program X-001 ...
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bioRxiv - Genomics 2022Quote: ... 5 million cells were transfected with 10ug of plasmid DNA in one 100- uL cuvette using the Amaxa® Cell Line Nucleofector® Kit T (Lonza), program X-001 ...
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bioRxiv - Molecular Biology 2022Quote: ... Electroporation of 5×106 HL-60 cells was performed with AIO-GFP HAX1 LCRC1 and AIO-GFP HAX1 LCRC2 using CLB-Transfection™ Kit (Lonza, Austria) and CLB-Transfection™ System (Lonza ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lines were cultures for no more than 30 passages and routinely tested for mycoplasma contamination using MycoAlert Mycoplasma Detection Kit (Lonza, Slough, UK).
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bioRxiv - Microbiology 2022Quote: ... 1.2E6 BHK21 cells were nucleofected with 2 µg of Spike plasmid using an Amaxa Nucleofector II with cell line kit L (Lonza #VCA-1005) and program A-031 ...
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bioRxiv - Neuroscience 2022Quote: ... All cell lines were verified to be mycoplasma free by first culturing the cells in antibiotic-free media and then subjected to a mycoplasma tested using MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza, UK).
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bioRxiv - Cell Biology 2022Quote: ... 1uM siRNA was electroporated into cells using the Amaxa Nucleofector II device and Cell Line Nucleofector Kit V (Lonza Bioscience, VCA-1003). B16-F10 cells were electroporated with program P-020 ...
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bioRxiv - Microbiology 2020Quote: ... cell lines were authenticated using STR profiling (Eurofins Genomics) and monitored for mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza Rockland, USA). Cells were cultured at 37 °C and 5 % CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Immunology 2020Quote: ... 106 cells per condition were nucleofected with 4 µg of DNA using the Amaxa Nucleofector II (Lonza, Kit R; program R-024). Nucleofected cells were allowed to recover for 24-48 hours before sorting for GFP positivity and lack of CD56 expression.
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bioRxiv - Microbiology 2021Quote: ... the Sleeping Beauty transposase system [37] was adapted to electroporate Jurkat cells using the Lonza SE Cell Line kit (Lonza, V4SC-1960). The pSBbi-RP plasmid was a gift from Eric Kowarz (Addgene plasmid #60513 ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lines are cultivated at 37°C with 5% CO2 and were tested every 3 months for mycoplasma contamination using Universal Mycoplasma detection kit (ATCC, 30-1012K) or MycoAlert Plus Mycoplasma Detection Ki (Lonza, LT07-701). Cell lines were used no more than 30 passages.
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bioRxiv - Systems Biology 2022Quote: ... All cells were grown at 37°C in 5% CO2 and regularly checked for mycoplasma (MycoAlert mycoplasma detection kit, Lonza, Basel, Switzerland). Identity of all cell lines was confirmed by STR analysis (Leibniz Institute DSMZ GmbH ...
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bioRxiv - Immunology 2019Quote: ... as a control two days prior to adoptive T cell therapy using the P3 Primary Cell 4D-Nucleofector X™ Kit (Lonza Bioscience) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... 2×105 cells were co-transfected with 2ug of the Cas9/sgRNA vector PxHF1* and 4 ul of ssODN HDR template (20 uM) using a Lonza X-Unit Nucleofector with P3 buffer kit (Lonza #V4XP-3032). Four days following transfection ...
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bioRxiv - Cell Biology 2020Quote: ... and the GFP-K17ΔNLS (Hobbs et al., 2015) were nucleofected into HeLa cells using SE Cell Line 4D X nucleofector Kit S (Lonza #V4XC-1032) with setting DS-138 ...
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bioRxiv - Neuroscience 2021Quote: ... Single-cell suspension of hiPSCs was nucleofected with 5 μg of the generated SpCas9-sgRNA plasmid using the P3 Primary Cell 4D-Nucleofector Kit (Lonza, #V4XP-3024) in combination with the 4D Nucleofector Unit X (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... All cell lines were routinely tested for the absence of mycoplasma using the MycoAlert Plus Mycoplasma Detection Kit from Lonza (Walkersville, MD).