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Citations for Lonza :
2201 - 2250 of 2465 citations for Rat Claudin 1 CLDN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... Horizontal electrophoresis of DNA was carried out using 1% Seakem LE agarose gels (Lonza, Basel, Switzerland). All cloning and molecular biology experiments were carried out according to established protocols ...
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bioRxiv - Bioengineering 2020Quote: Liver-Chips were stained in the upper channel with AdipoRed (1:40 dilution in PBS, Lonza) to visualize lipid droplet accumulation ...
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bioRxiv - Cancer Biology 2020Quote: ... Between 1×10^3 – 5×10^4 cells were re-suspended in 20μl Buffer P3 (Lonza) per reaction and quickly added to the Eppendorf tube containing the CRISPR/Cas9 gRNA RNP complex ...
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bioRxiv - Immunology 2022Quote: ... 1 × 106 cells were transfected with 8 μg ScaI-linearized plasmid using the Amaxa Nucleofector (Lonza) SF kit on program DS-113 ...
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bioRxiv - Synthetic Biology 2020Quote: ... then embedded in a lateral orientation in 1% low melting point agarose (NuSieve GTG agarose, Lonza), and allowed to polymerize in with cover glass (no ...
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bioRxiv - Immunology 2019Quote: ... This vector was electroporated into 1×10 B16.F10.Ova cells suspended in Solution V (Lonza) using the Amaxa 2B nucleofector (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... Chromosomes were separated by pulsed-field electrophoresis for 260 hours in 1% Seakem Gold agarose (Lonza) at 1.5 V/cm in a CHEF-DRII system (Biorad ...
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bioRxiv - Genetics 2019Quote: 1 liter of Sf9 insect cells grown in Spinner Cultures in BioWhitaker Insect-XPRESS media (Lonza) supplemented with 5% FBS (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: VEGF treatment of HdLECs: Confluent HdLEC monolayers were starved overnight in 1% FBS in EBM2 (Lonza) or in human endothelial SFM (Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 2μl of this was then used to transfect 1×106 promastigotes using a 4D nucleofector (Lonza). Parasites used for transfections were ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were plated on 1% gelatin-coated plates and maintained in plating medium [68% DMEM (Lonza), 17% 199 (Sigma) ...
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bioRxiv - Physiology 2022Quote: ... Cleared lysates were loaded into wells of the SDS-agarose gels (1% SeaKem Gold Agarose [Lonza, Basel ...
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bioRxiv - Microbiology 2022Quote: ... Human bronchial epithelial cells (NuLi-1) were cultured as previously described (19) in BEGM (Lonza, USA) with 1.15 mM calcium chloride and maintained as for hTCEpi ...
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bioRxiv - Molecular Biology 2022Quote: ... ASO (20 µM) was delivered into cells (1 × 106) by electroporation in a Nucleofector device (Lonza) using the Amaxa Cell Line Nucleofector Kit V (Lonza #VCA-1003 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The human monocyte THP-1 cells were cultured in RPMI 1640 media from Lonza (NSW, Australia), containing 4.5 g/L D-glucose and supplemented with 2 mM GlutaMax ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% FBS and 1% antibiotic-antimycotic and endothelial cell growth supplement (#CC-4176, Lonza) to 80% confluency before passaging (up to passage number 8) ...
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Starvation resistant cavefish reveal conserved mechanisms of starvation-induced hepatic lipotoxicitybioRxiv - Cell Biology 2024Quote: ... and mounted in 1% Low-Melt Agarose containing 0.02% Tricaine MS-222 (50080; Lonza, Basel, Switzerland) and imaged on a glass-bottomed FluoroDish (FD3510-100 ...
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: HM-1 ES cells were transfected with the resulting vector using a Nucleofector®-4D (Lonza) with the P3 Primary Cell X kit (Lonza ...
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bioRxiv - Genomics 2023Quote: ... The gDNA integrity was evaluated with pulsed-field gel electrophoresis SeaKem® GOLD Agarose 1% (Lonza), using the Pippin Pulse (Sage Science) ...
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bioRxiv - Bioengineering 2023Quote: ... Electroporation in Hepa 1-6 cells was carried out with a 4D Nucleofector X Unit (Lonza) using the CM-138 program as described in (34) ...
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bioRxiv - Molecular Biology 2024Quote: ... ∼300 pairs of ovaries were dissected from 3-6 day old females in 1× PBS (Lonza) with 1 mM DTT (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... at 1:50 dilution and cultured in a mixture of endothelial cell growth medium (EGM, Lonza) and hepatocyte culture media (HCM ...
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bioRxiv - Neuroscience 2020Quote: ... 6 µg of total plasmid DNA (2 µg for each of the three episomal vectors) were electroporated into 5×10^5 fibroblasts with the Amaxaμ Human Dermal Fibroblast Nucleofectorμ Kit (Lonza, Basel, Switzerland; VDP-1001). The fibroblasts were further cultured for 6 days to allow them to recover ...
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bioRxiv - Cell Biology 2021Quote: MLIV patient fibroblasts were grown for 2-3 days until 80-90% confluent at 37°C and prepared for electroporation with approximately 50nM of ON-TARGETplus siRNA SMARTpool reagents from Horizon Cell Solutions (Dharmacon) according to manufacturer instructions using the Amaxa P2 Primary Cell 4D-Nucleofector X Kit (Lonza, Cat. No. V4XP-2032) with nucleocuvette strips in a Lonza 4D-Nucleofector X Unit ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were also transfected with electroporation using Amaxa™ Nucleofector Kit R (Cat.no.VCA-100) and the Nucleofector™II device (program W-001, Lonza Group AG, Cologne, Germany) as suggested by the manufacturer.
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bioRxiv - Developmental Biology 2022Quote: Generation of Foxa2 knockout cell lines was performed by electroporating two different guides cloned into the pX458 vector (Ran et al., 2013) with the AMAXA nucleofector kit (Lonza Cat no. VPH-1001). Cells were sorted for GFP as single cells one day after electroporation ...
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bioRxiv - Immunology 2020Quote: ... 1.3×10^5 C25cl48 cells/reaction were electroporated with a ribonucleoprotein consisting of 125 pmol TrueCut Cas9 Protein v2 (A36498) + 150 pmol gRNA + 120 pmol ssDNA using P5 Primary Cell 4D-Nucleofector X Kit S (Lonza, Walkersville, MD, V4XP-5032) in a 16-well Nucleocuvette Strip and the Amaxa 4D-Nucleofector X Unit (Lonza ...
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bioRxiv - Cancer Biology 2019Quote: ... and 3-4 hours later were transfected with 2 μg of pBabe KRAS WT and pBabe KRAS G12D or pBabe KRAS Q61H plasmid DNA by electroporation using the Amaxa® Human T Cell Nucleofector® Kit (Lonza, Basel, Switzerland) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The cell line was authenticated through short tandem repeat analysis and tested for mycoplasma contamination every 10 passages using the MycoAlert Mycoplasma Detection Kit (Lonza Group Ltd, Basel, Switzerland). PDGFR-BiFC and myr-Venus stable cells were cultured in RPMI growth medium [RPMI 1640 (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: All cell lines were cultured at 37°C and 5% CO2 and tested for mycoplasma contamination using a MycoAlert® mycoplasma detection kit (Lonza, cat: LT07-218). Only mycoplasma-negative cells were used.
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bioRxiv - Bioengineering 2023Quote: The murine myoblast cell line C2C12 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and was tested for mycoplasma (MycoAlert™ Mycoplasma Detection Kit, Lonza AG, Basel, Switzerland). Cells were cultured in monolayer at 37 °C in a 5% CO2-containing humidified atmosphere in complete growth medium (GM) ...
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bioRxiv - Cancer Biology 2023Quote: NPE cells were transfected by electroporation using a Lonza® Nucleofector 2b device and the Lonza® Mouse Neural Stem Cell Nucleofector Kit (Lonza; #VPG-1004) according to the manufacturer’s instructions using the T-030 pulse code.
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bioRxiv - Cancer Biology 2023Quote: CD4 SMARTA T cells were isolated from naïve mice and were washed and resuspended in 20 μl buffer P3 (P3 Primary Cell 4D-Nucleofector X Kit S, Lonza, cat. no. V4XP-3032). Between 105 to 107 cells were used per nucleofection ...
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bioRxiv - Synthetic Biology 2023Quote: ... CD22-deleted clones modified to express PD-L1 (LZRS PD-L1) or CD200 (PB-EF-1α-intron CD200, pCMV-hyPBase, SF buffer kit V4XC-2032, Lonza 4D Nucleofector, code DN100). Transposition reactions used 1-2 μg of vector DNA and 0.5-1.0 μg of pCMV-hyPBase transposase DNA ...
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bioRxiv - Developmental Biology 2023Quote: ... mESCs were transfected with the pre-assembled fRNP pool by electroporation (program: OP315 or CD112) using an SE Cell line 4D-Nucleofector™ X kit (Lonza, Catalog#: V4XC-1024). The electroporated cells were plated in Nunc Glass Bottom Dishes (Thermo Fisher ...
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bioRxiv - Genomics 2023Quote: ... All iPSCs were karyotypically validated via g-banded karyotyping (WiCell) and are regularly screened for mycoplasm via MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza Cat # 75860-362). Two major approaches to generating neurons in a dish are based on overexpression of proneural factors combined with chemicals from iPSCs differentiation or directly converted from fibroblasts ...
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bioRxiv - Cell Biology 2023Quote: ... to aid complete removal of PBS before resuspension in P3 Nucleofection Solution (room temperature; P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza; cat. no., V4XP-3032). A total of 20 μl solution was used to resuspend 80,000 cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were maintained at 37°C with 5% CO2 and were regularly tested for Mycoplasma contamination using the MycoAlert PLUS kit (Lonza, Cat. No. LT07 −705).
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bioRxiv - Molecular Biology 2024Quote: ... counted and 1×106 cells electroporated using the CB-150 program of the 4D-Nucleofector™ System and the P3 Primary Cell 4D-Nucleofector® X Kit L (Lonza, #V4XP-3024) according the manufactures protocol and plated in E8+RI ...
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bioRxiv - Immunology 2024Quote: ... For transient transfection 1e6 Jurkat cells were transfected with 2 μg DNA using the LONZA electroporator (program X-001) and Nucleofector Kit V (Lonza, catalog no. VCA-1003) 24 h prior to imaging ...
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bioRxiv - Cancer Biology 2022Quote: ... where 1×106 4T1 cells in 50 µL of phosphate-buffered saline (PBS, Lonza, cat. 17-512F) were subcutaneously (s.c. ...
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bioRxiv - Cell Biology 2022Quote: ... HCFs were then cultured in supplemented serum-free media (SSFM; 1 mM sodium pyruvate (Lonza; Basel, Switzerland), 2mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... 75 ng of these substrates were electroporated into 200,000 cells with 1 ug of pMAX-GFP (Lonza) using the Neon system (Invitrogen ...
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bioRxiv - Genomics 2019Quote: ... TetO-CDC6 mESCs were cultured on 0.1% gelatin-coated plates in Dulbecco’s modified Eagle’s medium (DMEM) with Ultraglutamine 1 and 4.5 g/L glucose (Lonza) supplemented with 15% FBS (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... 1-10 million activated P14 cells were re-suspended in 20 μL primary cell nucleofection solution (Lonza), then mixed and incubated with the crRNA/Cas9 mix for 2 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The overlay medium contained either DMEM with 2% FBS and 1% sea-plaque agarose (Lonza, Walkersville, MD) in the case of in vitro samples or Opti-MEM with 2% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... After 1 h the medium was carefully replaces with supplemented hepatocyte maintenance medium (HMM) (#MP250, Lonza, Swizerland) until stimulations ...
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bioRxiv - Cell Biology 2020Quote: ... were cultured at 1000-1500 cells/mm2 on 1% gelatin-coated dish in EGM-2 medium (Lonza) and used before passage 9 ...
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bioRxiv - Developmental Biology 2019Quote: ... supplemented with 10% (v/v) fetal bovine serum (HyClone) and 1% (v/v) antibiotic antimycotic solution (Lonza). The cells were transfected with plasmid (pCMV6-entry from Origene ...
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bioRxiv - Biophysics 2019Quote: ... Approximately 1 to 1.5 × 106 hPSCs were washed in DPBS and resuspended in P3 primary buffer (Lonza) with 1 μg gRNA ...