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Citations for Lonza :
2101 - 2150 of 2363 citations for Mouse Muscleblind Like Protein 1 MBNL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... the cells were then additionally nucleofected with ribonuclease complex of ITGB2 sgRNA and Cas9 using P3 Primary Cell 4D-Nucleofector™ X Kit S (Lonza, V4XP-3032) using 4D-Nucleofector (Lonza ...
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bioRxiv - Molecular Biology 2022Quote: ... and the gRNA vector PX330-EN1082 using nucleofection with the Amaxa 4D-Nucleofector X-Unit and the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, V4XP-3024 KT) as described above ...
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bioRxiv - Molecular Biology 2022Quote: ... was transfected with the targeting vector pUC19-ITR-NeoR-ITR-3xCTCF-LacO and the gRNA vector pX330-chr15_LacO_gRNA/Cas9 using nucleofection with the Amaxa 4D-Nucleofector X-Unit and the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, V4XP-3024 KT) as described for the Tir1 integration ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected with the targeting vector pMK-3xCTCF-TetO-Rox-PuroR-Rox and the gRNA vector PX459-chr15_gRNA/Cas9 using nucleofection with the Amaxa 4D-Nucleofector X-Unit and the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, V4XP-3024 KT). 2×106 cells were nucleofected with 1 μg TetO targeting vector and 1 µg of PX459-ch15_gRNA/Cas9 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell pellets of 1E6 cells were resuspended with 20ul supplemented nucleofector solution of the P3 Primary Cell 4D-nucleofector® X Kit S (Lonza, V4XP-3032) and 2ug plasmid (1ug/ul) ...
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bioRxiv - Immunology 2022Quote: ... Activated cells were nuclofected with 100 µM AhR or non-targeting (NT1) siRNA (ON-TARGETplus SMART pool, Dharmacon) using the Amaxa Human T cell Nucleofector Kit (Lonza, Walkersville, MD, USA), according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... we transfected HeLa cells with SNAP-tagged CENP-A (generous gift from Dan Foltz) in combination with either empty vector or GFP-CENP- C using the Amaxa Nucleofector kit R (Lonza Bioscience, Walkersville, MD) per instructions.
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: SQD9 cells were transfected with PX459 using Nucleofector™ 2b Device and Cell Line Nucleofector™ Kit L (Lonza, #AAB-1001, #VACA-1005). Cas9-expressing cells were selected with 0.625 µg/mL puromycin (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... Next 1 × 106 cells resuspended in 93 μL of P3 Primary Cell Nucleofector Solution (P3 Primary Cell 4D-Nucleofector X Kit, Lonza, Catalog #: V4XP-3024) were mixed with 7 μL of RNP and 12 μg of single-stranded oligodeoxynucleotide (ssODN ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cell line identities were confirmed by short tandem repeat profiling.77 Cell cultures were routinely tested for Mycoplasma contamination using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza Bioscience, cat # LT07) according to manufacturer protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The RNP complex was mixed with 8 x 10e5 GBM1 cells resuspended in 100 μl of SG solution from the SG Cell Line 4D-NucleofectorTM X Kit (Lonza, Cat# V4XC-3024). This entire mix was then placed in a NucleocuvetteTM and was nucleofected using a Lonza Amaxa 4D-Nucleofector TM and the EN138 nucleofection programme ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated at 37°C in a humidified incubator with 5% CO2 and frequently tested for mycoplasma contamination using MycoAlert™ detection kit (Lonza, LT07-118).
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bioRxiv - Cancer Biology 2023Quote: Electroporation of multiplex CRISPR-Cas9 pX330 plasmids into normal bronchial epithelial cells was achieved using the P3 Primary Cell 4D-Nucleofector® X Kit L (Lonza, V4XP-3024) and 4D-Nucleofector™ X Unit (Lonza) ...
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bioRxiv - Neuroscience 2023Quote: ... into control and disease related iPSC lines in Amaxa® 4D- Nucleofector® using P4 Primary Cell 4D-Nucleofector® X Kit (Lonza) with the CM133 program ...
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bioRxiv - Immunology 2024Quote: ... MC38 Pdl1 KO cells were generated through CRISPR Cas9-gRNA RNP-directed deletion by using a Lonza 4D-Nucleofector and a SF Cell Line 4D-Nucleofector X Kit S (Lonza, cat. V4XC-2032). In brief ...
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bioRxiv - Immunology 2024Quote: ... The nucleofection was conducted using a Lonza 4D-Nucleofector and a P3 Primary Cell 4D-Nucleofector X Kit S (Lonza, cat. V4XP-3032) with the same protocol described earlier ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured at 37°C with 5% CO2 and were regularly tested for mycoplasma contamination using the MycoAlert® PLUS Mycoplasma Detection Kit (Lonza, LT07-703), and authenticated by Microsynth ...
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bioRxiv - Bioengineering 2023Quote: ... PiggyBac transposon constructs and RNA encoding transposase were introduced into T cells by electroporation using a Lonza 4D-Nucleovector instrument and the P3 Primary Cell Kit (Lonza, Cat. # V4XP-3032). Electroporated T cells were immediately seeded into 24-well G-Rex plates (Wilson Wolf ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmids expressing ETS1 sgRNAs (see Supplemental Table S1) were co-electroporated into the dCas9-VP64-expressing cells using the AmaxaTM Cell Line Nucleofector™ Kit R (Lonza, VCA-1001) and further subjected to puromycin selection and single-cell cloning ...
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bioRxiv - Neuroscience 2024Quote: ... sgRNA plasmids validated by Sanger sequencing were transfected into SH-SY5Y cells using nucleofection (SF Cell Line 4D-Nucelofector X kit, Lonza, Germany, V4XC-2012) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Drug solutions were removed and wells washed twice with 1 x phosphate buffered saline (PBS; Lonza). Fresh Complete
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bioRxiv - Bioengineering 2019Quote: ... they were partially embedded in a thin layer of 1% low-melting point agarose (SeaPlaque, Lonza) dissolved in physiological saline ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were obtained from Lonza and maintained in endothelial cell basal medium-2 plus 1% FBS and essential growth factors (Lonza).
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bioRxiv - Genomics 2020Quote: ... G8PPD-coding plasmid (1 μg) was mixed with re-suspended K562 cells and nucleofected by Lonza 4D nucleofector with program FF-120 ...
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bioRxiv - Immunology 2020Quote: ... and CD59 (deficient HAP-1 cells) (21) were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM; Lonza) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2019Quote: ... murine lymphocytes were resuspended at 3.5×106 cells/mL in supplemented HL-1 media (Lonza, UK) (1% L-glutamine ...
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bioRxiv - Synthetic Biology 2021Quote: ... Horizontal electrophoresis of DNA was carried out using 1% Seakem LE agarose gels (Lonza, Basel, Switzerland). All cloning and molecular biology experiments were carried out according to established protocols ...
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bioRxiv - Bioengineering 2020Quote: Liver-Chips were stained in the upper channel with AdipoRed (1:40 dilution in PBS, Lonza) to visualize lipid droplet accumulation ...
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bioRxiv - Cancer Biology 2020Quote: ... Between 1×10^3 – 5×10^4 cells were re-suspended in 20μl Buffer P3 (Lonza) per reaction and quickly added to the Eppendorf tube containing the CRISPR/Cas9 gRNA RNP complex ...
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bioRxiv - Immunology 2022Quote: ... 1 × 106 cells were transfected with 8 μg ScaI-linearized plasmid using the Amaxa Nucleofector (Lonza) SF kit on program DS-113 ...
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bioRxiv - Synthetic Biology 2020Quote: ... then embedded in a lateral orientation in 1% low melting point agarose (NuSieve GTG agarose, Lonza), and allowed to polymerize in with cover glass (no ...
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bioRxiv - Immunology 2019Quote: ... This vector was electroporated into 1×10 B16.F10.Ova cells suspended in Solution V (Lonza) using the Amaxa 2B nucleofector (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... Chromosomes were separated by pulsed-field electrophoresis for 260 hours in 1% Seakem Gold agarose (Lonza) at 1.5 V/cm in a CHEF-DRII system (Biorad ...
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bioRxiv - Genetics 2019Quote: 1 liter of Sf9 insect cells grown in Spinner Cultures in BioWhitaker Insect-XPRESS media (Lonza) supplemented with 5% FBS (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: VEGF treatment of HdLECs: Confluent HdLEC monolayers were starved overnight in 1% FBS in EBM2 (Lonza) or in human endothelial SFM (Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 2μl of this was then used to transfect 1×106 promastigotes using a 4D nucleofector (Lonza). Parasites used for transfections were ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were plated on 1% gelatin-coated plates and maintained in plating medium [68% DMEM (Lonza), 17% 199 (Sigma) ...
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bioRxiv - Physiology 2022Quote: ... Cleared lysates were loaded into wells of the SDS-agarose gels (1% SeaKem Gold Agarose [Lonza, Basel ...
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bioRxiv - Microbiology 2022Quote: ... Human bronchial epithelial cells (NuLi-1) were cultured as previously described (19) in BEGM (Lonza, USA) with 1.15 mM calcium chloride and maintained as for hTCEpi ...
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bioRxiv - Molecular Biology 2022Quote: ... ASO (20 µM) was delivered into cells (1 × 106) by electroporation in a Nucleofector device (Lonza) using the Amaxa Cell Line Nucleofector Kit V (Lonza #VCA-1003 ...
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: HM-1 ES cells were transfected with the resulting vector using a Nucleofector®-4D (Lonza) with the P3 Primary Cell X kit (Lonza ...
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bioRxiv - Genomics 2023Quote: ... The gDNA integrity was evaluated with pulsed-field gel electrophoresis SeaKem® GOLD Agarose 1% (Lonza), using the Pippin Pulse (Sage Science) ...
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bioRxiv - Bioengineering 2023Quote: ... Electroporation in Hepa 1-6 cells was carried out with a 4D Nucleofector X Unit (Lonza) using the CM-138 program as described in (34) ...
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Starvation resistant cavefish reveal conserved mechanisms of starvation-induced hepatic lipotoxicitybioRxiv - Cell Biology 2024Quote: ... and mounted in 1% Low-Melt Agarose containing 0.02% Tricaine MS-222 (50080; Lonza, Basel, Switzerland) and imaged on a glass-bottomed FluoroDish (FD3510-100 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The human monocyte THP-1 cells were cultured in RPMI 1640 media from Lonza (NSW, Australia), containing 4.5 g/L D-glucose and supplemented with 2 mM GlutaMax ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% FBS and 1% antibiotic-antimycotic and endothelial cell growth supplement (#CC-4176, Lonza) to 80% confluency before passaging (up to passage number 8) ...
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bioRxiv - Neuroscience 2020Quote: ... 6 µg of total plasmid DNA (2 µg for each of the three episomal vectors) were electroporated into 5×10^5 fibroblasts with the Amaxaμ Human Dermal Fibroblast Nucleofectorμ Kit (Lonza, Basel, Switzerland; VDP-1001). The fibroblasts were further cultured for 6 days to allow them to recover ...
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bioRxiv - Cell Biology 2021Quote: MLIV patient fibroblasts were grown for 2-3 days until 80-90% confluent at 37°C and prepared for electroporation with approximately 50nM of ON-TARGETplus siRNA SMARTpool reagents from Horizon Cell Solutions (Dharmacon) according to manufacturer instructions using the Amaxa P2 Primary Cell 4D-Nucleofector X Kit (Lonza, Cat. No. V4XP-2032) with nucleocuvette strips in a Lonza 4D-Nucleofector X Unit ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were also transfected with electroporation using Amaxa™ Nucleofector Kit R (Cat.no.VCA-100) and the Nucleofector™II device (program W-001, Lonza Group AG, Cologne, Germany) as suggested by the manufacturer.
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bioRxiv - Developmental Biology 2022Quote: Generation of Foxa2 knockout cell lines was performed by electroporating two different guides cloned into the pX458 vector (Ran et al., 2013) with the AMAXA nucleofector kit (Lonza Cat no. VPH-1001). Cells were sorted for GFP as single cells one day after electroporation ...