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Citations for Lonza :
2101 - 2150 of 2664 citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Cells were mixed with complete p3 electroporation solution (from a Lonza 4D nucleofection kit; Lonza Group AG, Basel, Switzerland) followed by addition of the RNP complex and 500 pmoles of donor ssODNA (Alt-R HDR ...
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bioRxiv - Cell Biology 2022Quote: A sub-cloned population of BEL-A cells (1.0 × 105) were resuspended in CD34+ nucleofection kit buffer (Lonza Biosciences) containing 18 pmol Cas9 ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: ... The resuspended cells were immediately transferred into the cuvette of the P3 Primary Cell Nucleofector Kit (Lonza; V4SP-3096) and electroporated using code EH-100 on the Lonza 4D-Nucleofector ...
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bioRxiv - Systems Biology 2022Quote: ... Electroporation mix was performed using the Lonza Amaxa SE Nucleofection 4D kit in the 100μl format with the 4D-NucleofectorTM X Unit (Lonza) machine ...
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bioRxiv - Bioengineering 2023Quote: ... and 40 pmol of single-stranded oligonucleotide donor DNA (ssODN) were nucleofected to 0.25 × 106 HEK293T cells using the SF Cell Line 4D-Nucleofector kit (Lonza) following the pulse program of CM-130 ...
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bioRxiv - Microbiology 2023Quote: ... All cell lines used in this study were regularly screened for Mycoplasma contamination with the MycoAlert Detection Kit (Lonza).
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bioRxiv - Cell Biology 2023Quote: ... and mN designates the 2’-O-methylribonucleotides) was transfected into HAP1 cells (1.0 × 106 cells) by Nucleofector Kit V (Lonza) with a Nucleofector device (Lonza ...
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bioRxiv - Developmental Biology 2024Quote: ... Mycoplasma testing was carried out by DAPI staining of fixed cultures and using the mycoalert mycoplasma detection kit (Lonza). We confirm that the cell lines used in this study were free of mycoplasma and aerobic bacteria and fungi.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were confirmed as negative for mycoplasma contamination by using a Lonza Mycoplasma Detection Kit (Lonza Bioscience, Durham, NC) before injection ...
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bioRxiv - Synthetic Biology 2024Quote: ... K562 cells were electroporated with pDNA using the SF cell line 96-well Nucleofector kit (Lonza, Catalog#V4SC-2960) or SF Cell Line 384-well Nucleofector Kit (Lonza ...
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bioRxiv - Genetics 2024Quote: ... Testing for mycoplasma contamination was done robotically with the MycoAlert Mycoplasma Detection kit mycoplasma luminescent assay (Lonza, #LT107-318) and the accompanying MycoAlert Assay Control Set (Lonza ...
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bioRxiv - Immunology 2024Quote: ... All cell lines were tested negative for mycoplasma contamination using the Mycoalert™ Mycoplasma Detection Kit (Lonza Bioscience Solutions) prior to use.
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bioRxiv - Immunology 2024Quote: ... Cell lines were screened for mycoplasma via the MycoAlertTM Mycoplasma Detection Kit (Lonza, Walkersville, MD; Cat No. LT07-318).
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bioRxiv - Neuroscience 2024Quote: Nucleofections were done on cortical neurons using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, LZ-V4XP-3024), on the day of preparation and dissociation (DIV 0) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were verified as negative for mycoplasma by testing with the MycoAlert Mycoplasma Detection Assay kit (Lonza, LT07–318) per the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Cell culture media supernatant was tested for mycoplasma contamination every 4lJweeks using the MycoAlert PLUS mycoplasma detection kit (Lonza) and all tests were negative throughout the experiments.
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bioRxiv - Genomics 2024Quote: ... Mycoplasma contamination testing was routinely performed in the cell lines while in culture (MycoAlert™ Mycoplasma Detection Kit, Lonza). LNCaP cells were seeded at 80% confluence and grown for 3 days in phenol red-free RPMI Medium with 5% charcoal-stripped Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell cultures were routinely tested for the presence of mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza, USA) following manufacturer’s protocol.
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bioRxiv - Bioengineering 2024Quote: Nucleofection of effector plasmids was performed with SF Cell Line 4D-Nucleofector X Kit S following the manufacturer’s protocol (4D X-unit, FF-120 program, Lonza). PB Tet-On dCas9 K562 cell line (with CD81 guide array ...
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bioRxiv - Cell Biology 2024Quote: ... All the cell lines were confirmed to be free of mycoplasma contamination by using a Mycoalert PLUS kit (Lonza). To inhibit transcription ...
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bioRxiv - Cell Biology 2024Quote: ... the cell pellet was resuspended in 20 µL of pre-warmed P3 primary cell solution (Lonza Kit, V4XP-3032), 2.5 µL RNP complex ...
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bioRxiv - Cancer Biology 2024Quote: ... CRISPR-RNP complexes were delivered to primary medulloblastoma cells using the P3 Primary Cell Nucleofection Kit (Lonza #V4XP-3032) for the 4D Nucleofector X (Lonza #AAF-1003X) ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell cultures were regularly checked for mycoplasma contamination with the MycoAlert® Mycoplasma Detection Kit (LT07-118, Lonza) according to the manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2024Quote: ... HLECs were grown on culture dishes or glass slides coated with 0.2% gelatin and were maintained in EGM-2 EC Growth Medium-2 Bullet Kit (Lonza). All experiments were conducted using cells until passage (P ...
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bioRxiv - Cell Biology 2024Quote: ... All the iPSC lines were routinely tested for mycoplasma contamination by using MycoAlert™ Mycoplasma Detection Kit by Lonza, according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... All cell lines were routinely tested for mycoplasma contamination using the MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza LT07-710).
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Tuning trophoblast invasion in a gelatin hydrogel via soluble cues from the maternal-fetal interfacebioRxiv - Bioengineering 2020Quote: ... Routine mycoplasma testing was performed every 6 months to ensure cell quality using the MycoAlert™ Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were maintained at 37°C and 5 % CO2 in a humidified atmosphere and routinely tested to be mycoplasma negative (MycoAlert Mycoplasma Detection Kit, Lonza). Cells were cultured no longer than 15 passages before experimental use.
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bioRxiv - Cancer Biology 2021Quote: ... A total of 500,000 cells were transfected using the using the mouse neural stem cell nucleofection kit (Lonza, VPG-1004) with 0.66 μg of the construct and 0.33 μg of transposase using the Lonza Amaxa Nucleofector I with protocol A-33 ...
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bioRxiv - Cell Biology 2020Quote: ... Modified MTEC culture media is comprised of small airway basal media (SABM) with selected components from SAGM bullet kit (Lonza) including Insulin ...
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bioRxiv - Cell Biology 2020Quote: U-2 OS cells stably expressing spdCas9-GFP were nuclofected with Amaxa® Cell Line Nucleofector® Kit V (Lonza). Cells were detached with 0.05% trypsin/EDTA and spun down by centrifugation at 1000rpm for 5 min ...
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bioRxiv - Immunology 2021Quote: ... WT RAW264.7 cells were subjected to two rounds of nucleofection with Cas9-RNPs using the SF Cell Line 4D-Nucleofector X Kit S (Lonza) and the 4D Nucleofector apparatus precisely as specified by the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... Constructs containing validated gRNAs were electroporated together with a vector expressing puromycin into hESCs using the P3 Primary Cell 4D-Nucleofector X kit (Lonza) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the RNP complexes were added to 1×105 cells in 40 μl of P3 nucleofection buffer from the 4D-Nucleofector X kit (Lonza). Half of the mixture was loaded to each well of a 16-well nucleofection cassette and nucleofected using the using E0-100 program with the 4D-Nucleofector Lonza Amaxa ...
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bioRxiv - Microbiology 2021Quote: ... were electroporated with 20 ug of guide plasmid and 60 ug of linearized repair plasmid using an Amaxa 4D electroporator and P3 Primary cell 4D Nucleofector X Kit L (Lonza) using programme FP158 as described (Collins et al. ...
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bioRxiv - Immunology 2022Quote: ... a total of 1.6 x 108 cells were transfected with 2 μM of the corresponding ASO using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza) and the EO-115 program ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was removed and the cells were resuspended in 100 µL of nucleofector solution from P3 Primary Cell 4D-Nucleofector Kit (Lonza) per transfection ...
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bioRxiv - Cell Biology 2022Quote: ... 90 μL of nucleofection solution (16.2 μL of Supplement solution mixed with 73.8 μL of SF solution from SF Cell Line 4D-Nucleofector™ X Kit L) (Lonza) was mixed thoroughly with the cell pellet ...
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bioRxiv - Immunology 2021Quote: Control or transfected Lymphocytes (with Flag-wt-TDP-43 or Flag-NLS-mut-TDP-43 plasmid (1 μg) using nucleofection kit (Lonza)) were placed on coverslips (2 x 106 cells in sterile glass coverslips-Ø 12 mm ...
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bioRxiv - Genomics 2020Quote: 5 × 105 THP-1 cells were resuspended in 20µL nucleofection solution (16.4µL SG nucleofector solution + 3.6µL supplement 1) from the SG Cell Line 4D-Nucleofector™ X kit (Lonza, V4XC-3032). 300pmol PPP2R1A sgRNA (Synthego ...
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bioRxiv - Cancer Biology 2020Quote: Bioluminescent detection of cellular ATP as a measure of cell viability was undertaken using ViaLight® Plus Kit (Lonza Inc.) reagents ...
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bioRxiv - Bioengineering 2021Quote: ... Each chamber was connected to a reservoir that was pre-filled with 15 mL of warm serum-free EGM-2 (endothelial cell growth medium-2 without serum added from the kit, Lonza). ECFCs were harvested and resuspended in serum-free EGM-2 and added to the reservoirs to reach an overall cell density of 125,000 cells/mL ...
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bioRxiv - Genomics 2020Quote: ... 5 million cells were transfected with 5 μg of DNA FAIRE-STARR library plasmid using the Amaxa Nucleofector kit V (Lonza). For each condition ...
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bioRxiv - Systems Biology 2021Quote: ... and 2×106 iPSCs were mixed with pX459-sgRNA vector and ssDNA repair donor template and electroporated with Lonza 4D-Nucleofector system using the P3 Primary Cell Nucleofector Kit (Lonza). The iPSCs were then recovered in mTeSR1 medium for 48 hours before adding puromycin for 3-day selection ...
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bioRxiv - Microbiology 2021Quote: ... the transfected cells were co-cultured with BHK-21 cells (ATCC CCL-10) transfected with VSV-G using the SE cell Line 4D-Nucleofector X Kit L (Lonza) in DMEM without glutamine (Life Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... 200,000 cells were electroporated with 1000 ng adRNA plasmid or 48 pmol of IVT RNA using the Amaxa SF cell Line 4D-Nucleofector X kit (Lonza) as per the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... The cells were used up to passage number 12 and were tested for mycoplasma contamination (MycoAlertTM Mycoplasma Detection Kit, Lonza) at first and last passage number ...
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bioRxiv - Neuroscience 2021Quote: ... The efficacy of siRNA targeting Kdm6a was assessed by electroporation of neurons using a 4D-Nucleofector X Unit and the corresponding P3 Primary Cell nucleofection kit (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were maintained in 37 °C and 5% CO2 incubators and routinely tested negative for mycoplasma contamination using the MycoAlert Kit (Lonza).
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bioRxiv - Cancer Biology 2020Quote: ... Their identity was verified by using STR profiling (Eurofins) and lack of Mycoplasma infection was confirmed by Mycoalert Mycoplasma Detection Kit (Lonza). Cell lines were cultured in Dulbecco’s modified eagle’s medium (DMEM):F12-HAM (Sigma ...