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Citations for Lonza :
2101 - 2150 of 2449 citations for Chicken Metallothionein 1 MT1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and 1.2 μl of Alt-R® Cas9 Electroporation enhancer (100 μM, IDT) using P3 Primary Cell Nucleofector® 4D Kit and Nucleofector 4D system (Lonza). After electroporation ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were authenticated and routinely tested to be mycoplasma-free using the MycoAlert Mycoplasma Detection Kit (Lonza, Cat # LT07-710) every two months ...
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bioRxiv - Cancer Biology 2024Quote: ... Mycoplasma testing was performed on all cells when they were thawed and semi-regularly thereafter using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza LT07-703). Independent experiments were performed on cells treated with siRNA and/or compounds from separate passages of each cell line ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lines were authenticated by short tandem repeat analysis and routinely tested for mycoplasma using the MycoAlert mycoplasma detection kit (Lonza, #LT07-710).
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bioRxiv - Cell Biology 2024Quote: ... Cells were maintained at 37°C and 5% CO2 in 2D monolayer culture and confirmed as Mycoplasma negative (MycoAlert Mycoplasma Detection Kit, Lonza #LT07-701). Cells were maintained in DMEM medium (Corning #10-013-CV ...
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bioRxiv - Cell Biology 2024Quote: ... 8 x 105 H9 hESCs in single-cell suspension were resuspended in 100 µL electroporation buffer from the Human Stem Cell Nucleofector Solution 2 kit (Lonza, VPH-5022). Alt-R Cas9 Electroporation enhancer (1.1 µM ...
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bioRxiv - Systems Biology 2024Quote: ... HiFi Cas9 Nuclease V3 protein (IDT, 1081058) were introduced into low-passage dual-reporter ESCs using the Mouse Embyronic Stem Cell Nucleofector Kit (Lonza VPH-1001). ESCs were treated with 0.025% trypsin/1% EDTA (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... In microscopy experiments we used the same media but without phenol red to reduce background fluorescence (Lonza CC-3153 phenol-red free basal media supplemented with growth factors and other components from the Lonza CC4136 kit). T98G cells were purchased from ATCC ...
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bioRxiv - Cell Biology 2020Quote: ... Cells in passage 1 were trypsinized and resuspended (3 × 104 cells/mL) in BEGM** (Lonza, see Table S4 for details of media composition ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... 1 μg plasmid DNA was added and cells electroporated in a 4D-NucleofectorTM System (Lonza) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... ~1 μl of cells were mounted on EMM4S agarose pads (1.4% InCert™ Agarose, Lonza) and sealed under a coverslip with VALAP (Vaseline ...
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bioRxiv - Bioengineering 2020Quote: ... and the injury site was covered completely with a layer of 1% sterile SeaKem (Lonza) agarose ...
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bioRxiv - Cancer Biology 2020Quote: ... 1×106 Primary Human T cells were electroporated using the 4D-nucleofector (Lonza, Basel, Switzerland) and a P3 Primary Cell 4D-Nucleofector™ X Kit (V4XP-3032) ...
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bioRxiv - Cell Biology 2021Quote: Astrocytes were transfected with siRNAs (1-5nM) or plasmids (5µg) using a Nucleofector machine (Lonza) and the appropriate Lonza glial cell nucleofector solution ...
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bioRxiv - Immunology 2021Quote: ... . THP-1 cells were transfected with the plasmid using the Amaxa Nucleofector 2b device (Lonza) and the Human Monocyte Nucleofector Kit (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... Murine L929 cells (ATCC CCL-1) were maintained in Eagle’s minimal essential medium (MEM) (Lonza) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2019Quote: Murine L929 cells (ATCC CCL-1) were maintained in Eagle’s minimal essential medium (MEM) (Lonza) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... placed on Lo-Flo SD + 1% agarose pad (SeaKem ® Gold Agarose, Lonza, Rockland, ME) supplemented with 15 μg/mL nocodazole and 1 mM auxin ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were resuspended in P3 Primary Cell Nucleofector™ Solution containing Supplement 1 (Lonza, Switzerland) and 1 µg of chemically modified sgRNA (Synthego ...
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bioRxiv - Microbiology 2021Quote: ... 1% 10 mM HEPES in 0.85% NaCl (17-737E, Lonza, BioWhittaker®, Walkersville, MD, USA) and 100 U/ml Penicillin-100 µg/ml Streptomycin (15140-122 ...
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bioRxiv - Microbiology 2019Quote: ... cells were spotted onto pads made of 1% SeaKem LE Agarose (Lonza, Cat. No. 50000) in PYE and topped with a glass coverslip ...
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bioRxiv - Genomics 2021Quote: ... 100 units of potassium penicillin and 100 μg of streptomycin sulfate per 1 ml (Lonza). Four and eight T75 cell culture flasks were used for the ERS2312967 and ERS1870077 samples ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended at 1×106 cells/ml in XH media (X-Vivo 15 (Lonza) with 5% human serum ...
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bioRxiv - Immunology 2021Quote: ... 1% penicillin-streptomycin (penicillin 50 U/ml and streptomycin 50 μg/ml, final concentration; Lonza) at 37 °C with 5% CO2 ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 million hESCs were resuspended with 100uL Lonza Amaxa P3 nucleofection solution (Lonza V4XP-3024), the annealed ...
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bioRxiv - Immunology 2022Quote: ... For larger scale electroporation in the 1 ml scale of Nucleocuvette Cartridge (Lonza, V4LN-7002), reactions were scaled up 10 fold for cell suspension ...
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bioRxiv - Microbiology 2024Quote: ... Erythrocyte cultures were established in 6 well plates using HL-1 medium (Lonza, Basel, Switzerland) supplemented with 20% human serum type A+ (Interstate Blood Bank) ...
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bioRxiv - Bioengineering 2024Quote: ... For larger scale electroporation in the 1 ml scale of Nucleocuvette Cartridge (Lonza, V4LN-7002), reactions were scaled up 10-fold for cell suspension ...
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bioRxiv - Immunology 2023Quote: ... and cells were harvested on day 7 using 1 X PBS EDTA (Lonza, BE02-017F) for experiments.
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bioRxiv - Neuroscience 2023Quote: ... Those that were positive were individually mounted in 1% low melting point agarose (50100, Lonza) and imaged on a Zeiss LSM 980 confocal microscope using a 20x (NA=0.5 ...
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bioRxiv - Genomics 2023Quote: ... Both vectors were also transfected as background controls (1 µg) with pmaxGFP (9 µg, Lonza). 24 hours post nucleofection ...
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bioRxiv - Cancer Biology 2023Quote: All cultures were routinely (every 1-2 months) tested for mycoplasma contamination using MycoAlert (Lonza). Autosomal STR profiles for cell line authentication were performed by the University of Arizona Genetics Core.
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bioRxiv - Cancer Biology 2023Quote: ... and Panc-1 (ATCC #CRL-1469) were cultured in D10 media: DMEM (Lonza #12-614Q) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... 1% 10 mM HEPES in 0.85% NaCl (17-737E, Lonza, BioWhittaker®, Walkersville, MD, USA), 1% antibiotic-antimycotic ...
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bioRxiv - Neuroscience 2023Quote: ... After reaching 70% confluency by co-transfection with 1 µg of pmaxGFP control cDNA (Lonza), the transfected cells were cultured for an additional 48 h before use.
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bioRxiv - Cancer Biology 2023Quote: ... and 1 x 106 cells were resuspended in 100 μL of NHDF Nucleofector Solution (Lonza). The cell suspension was then combined with the CRISPR transfection solution and gently mixed prior to electroporation on an Amaxa Nucleofector 2b (Lonza ...
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bioRxiv - Developmental Biology 2024Quote: ... Gastrula or neurula were mounted in 1% low melting temperature agarose (SeaPlaque agarose, #50101, Lonza) in 0.1× MMR on a glass slide attached to a silicone isolator (1.2 mm ...
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bioRxiv - Molecular Biology 2024Quote: ... A 1.5 mm thick 16x16 cm 1% SeaKem Gold agarose (Lonza group ltd., Basel, Switzerland) gel was cast and used for electrophoresis ...
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bioRxiv - Cancer Biology 2021Quote: ... All cell lines used in this study tested negative for Mycoplasma contamination via MycoAlert™ PLUS Mycoplasma Detection Kit (LT07-710, Lonza, Basel, Switzerland).
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bioRxiv - Genomics 2020Quote: ... 2*106 TX1072 mESCs were electroporated with 2µg of each guide plasmid and 30pmol of the single stranded repair oligo using the P3 Primary Cell 4D-Nucleofector X Kit (V4XP-3024) with the Amaxa 4D Nucleofector system (Lonza, program CP-106) and plated on gelatin-coated 10cm dishes ...
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bioRxiv - Cancer Biology 2019Quote: ... PrEC cells were cultured in Prostate Epithelial Cell Basal Medium (PrEGM) supplemented with Prostate Epithelial Cell Growth Kit (Clonetics™ PrEGM™, BulletKit™, Lonza). All cell cultures were maintained at 37°C in an incubator with a controlled humidified atmosphere composed of 95% air and 5% CO2.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were tested biweekly during experiments using a mycoplasma detection kit to confirm absence of mycoplasma contamination using the Lonza MycoAlert® (Lonza #LT07-318). HCC1806 cells were grown in RPMI 1640 Medium (Life Technologies #11875093 ...
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bioRxiv - Immunology 2019Quote: ... two PX330-GFP vectors coding different CD80 sgRNA were electroporated into wild-type Raji cells (CD80+/CD86+/PD-L1-) using Cell Line Nucleofector Kit V (LONZA, catalog VACA-1003). Electroporated cells were recovered for two days at 37 °C/5% CO2 ...
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bioRxiv - Neuroscience 2019Quote: ... Cortical neurons from C57BL/6J and C57BL/6J Sorcs1flox/flox mice were electroporated with DNA just before plating using an AMAXA Nucleofector kit (Lonza, Cat #VPG-1001). Cortical neurons from C57BL/6J Sorcs1flox/flox mice were transfected at DIV7 using Effectene (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... All cells were tested bimonthly – every 2 months – to ensure mycoplasma-free cell culture using MycoAlert™ mycoplasma detection kit (Lonza, #LT07-418) and MycoAlert™ assay control set (#LT07-518) ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were routinely authenticated using short tandem repeat profiling and verified to be free of mycoplasma using the MycoAlert mycoplasma detection kit (Lonza Cat# LT07-418), by the QIMR Berghofer Medical Research Institute analytical facility (Brisbane ...
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bioRxiv - Cancer Biology 2020Quote: ... A total of 1.5×106 tumor cells (td-tomato MDA-MB-231 SORE6>GFP) were transfected with 10 μl of 20 μM siRNA stock solution using Nucleofector Kit V from Lonza (cat# VCA-1003) for 48 h ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... USA) and grown in endothelial basal medium (EBM) in combination with endothelial cell growth medium (EGM-2) bullet kit (Lonza, Walkersville, MD, USA). iCEC2 cells were a kind gift from Dr ...
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bioRxiv - Neuroscience 2022Quote: ... and pCXLE-hUL (#27080) were transfected into fibroblasts using a 4D-Nucleofector system with P2 Primary Cell 4D-Nucleofector X Kit (Lonza; program DT-130). Three to five weeks after reprogramming ...
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bioRxiv - Immunology 2022Quote: ... The Cas9 protein and sgRNA were electroporated into the T cells by using Amaxa 4D-Nucleofector System and P4 Primary Cell 4D-Nucleofector® X Kit S (Lonza, V4XP-4032) according to manufacturer’s instructions ...