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Citations for Lonza :
151 - 194 of 194 citations for Palmitic acid U 13C16 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... diluted in an equal volume of DMEM containing 20% FBS and 200 U/ml of penicillin/streptomycin (Lonza), and ∼1.5×104 hemolymph sporozoites were added per well ...
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bioRxiv - Cancer Biology 2024Quote: ... were separated on a PA-TBE gel and stained with GelStarTM Nucleic Acid Gel Stain (Lonza, 50535).
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed with 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffered saline solution (CC-5024, Lonza) before renewing the medium or passaging the cells ...
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bioRxiv - Microbiology 2021Quote: ... berghei ANKA using the parasite nucleofector II kit and the Nucleofector II device with the U-033 program (Lonza). Transfected parasites were injected intravenously into 5 - 7 weeks old female ddY mice ...
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bioRxiv - Biochemistry 2021Quote: ... then the ventricles were dissected and dissociated by serial digestion at 37°C with 0.44 mg/ml (6800 U) Worthington Type II collagenase (supplied by Lonza) and 0.6 mg/ml pancreatin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% tetracycline-free and heat inactivated fetal bovine serum (FBS) and 100 U/mL penicillin/streptomycin (Lonza). Cell lines were grown at 37 °C in a humidified atmosphere of 5% CO2 and were tested for absence of mycoplasma contamination.
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bioRxiv - Immunology 2024Quote: ... 107 schizonts were resuspended 100ul in human T cell transfection buffer containing 5μg digested DNA for transfection by U-33 program (Lonza).
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bioRxiv - Microbiology 2022Quote: ... and stained with 10 × SYBR Green I (SYBR® Green I Nucleic Acid Stain; Lonza, Rockland, ME, USA) in 1×TAE buffer for 3 min ...
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bioRxiv - Bioengineering 2023Quote: The DBM powders were decellularized in a solution of 0.05% trypsin with 0.02% ethylenediaminetetraacetic acid (trypsin/EDTA, Lonza) at 37 °C and 5% CO2 incubator under agitation for 24 hours (Figure 1c) ...
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bioRxiv - Cell Biology 2020Quote: U-2 OS cells stably expressing spdCas9-GFP were nuclofected with Amaxa® Cell Line Nucleofector® Kit V (Lonza). Cells were detached with 0.05% trypsin/EDTA and spun down by centrifugation at 1000rpm for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... 50 μl or 100 μl of homogenate from groups was plated on both YPD agar supplemented with 250 U/ml ,250 μg/ml penicillin-streptomycin (Lonza), 30 μg/ml gentamicin sulfate (BioWhittaker ...
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bioRxiv - Cancer Biology 2022Quote: ... Pmel-1 CD8+ T cells were activated by incubating with R10 media (RPMI containing 10 % FBS, 100 U/mL penicillin and 100 mg/mL streptomycin (all Lonza), 1x MEM NEAA ...
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bioRxiv - Cell Biology 2022Quote: ... HEK-293T and U-2OS (ATCC, Manassas, Virginia) cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: ... amazonensis promastigotes using the Human T-Cell Nucleofector kit and the Amaxa Nucleofector electroporator (program U-033, Lonza, Basel, Switzerland), for integration into the 18S rRNA locus within the nuclear DNA ...
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bioRxiv - Immunology 2024Quote: ... siRNAs were added to 2 µM and electroporation was performed using the program U-001 on the 3D-Nucleofector (Lonza). The siRNAs targeting Clever-1 ...
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bioRxiv - Bioengineering 2024Quote: ... at a 1:1 Target:T cell ratio in a 96-well U-bottom plates and 250 µl X-VIVO™ 15 (Lonza) supplemented with 5% human AB serum (Gemini Bio ...
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bioRxiv - Bioengineering 2024Quote: ... at a 1:1 Target:T cell ratio in 96-well U-bottom plates with 250 µl X-VIVO™ 15 (Lonza) supplemented with 5% human AB serum (Gemini Bio ...
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bioRxiv - Biophysics 2021Quote: ... 10 pmol of the cholesterol-modified reverse primer and 1μL 20X SYBR® Green I Nucleic Acid Stain (Lonza) were mixed with the KAPA2G Fast HotStart ReadyMix in a total volume of 20 μL ...
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bioRxiv - Cell Biology 2023Quote: ... 1xPenStrep (cat#XC-A4122, Biosera, Nuaille, France) and 1% nonessential amino acid (NEAA, cat#BE13-114E, Lonza, Basel, Switzerland) and maintained at 37 °C under 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... containing bone marrow mononuclear cells was washed in 5 ml basal medium (α – MEM containing 10% FBS and 100 U mL−1 penicillin and 100 μg mL−1 streptomycin; Lonza). All washing stages were at 400 x g for 5 minutes.
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bioRxiv - Cell Biology 2020Quote: ... containing bone marrow mononuclear cells was washed in basal medium (α-MEM containing 10% FBS and 100 U mL-1 penicillin and 100 µg mL-1 streptomycin; Lonza). Cells were subsequently either sorted using MACS or incubated with SNAs.
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bioRxiv - Systems Biology 2021Quote: ... complemented with 10% fetal bovine serum (Biosera, France) and Penicillin/Streptomycin (100 U/ml and 100 μg/ml, respectively - Lonza, Switzerland). Cells were seeded on poly-L-lysine pretreated (0.001% ...
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bioRxiv - Cell Biology 2024Quote: ... and 95% humidity in Human IntestiCult™ Organoid Growth Medium Human (IntestiCult OGMh) supplemented with 100 U/mL penicillin/streptomycin (Pen-Strep) (Lonza). Medium was replenished every second day ...
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bioRxiv - Microbiology 2020Quote: ... using the AMAXA Nucleofector 4D system (U-033 setting) and the P3 primary cell 4D-nucleofector X kit with the 16-well nucleocuvette strip (Lonza, V4XP-3032). Clones were obtained by FACS using the FACS Aria II sorter at the Stanford Shared FACS Facility for the brightest red parasites and single cloning the TdTomato+ enriched population by limiting dilution into 96-well plates.
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bioRxiv - Immunology 2020Quote: ... 1 × 106 BLaER1 cells were transfected with 2 μg plasmid DNA using the Human B Cell Nucleofector Kit and Nucleofector I device (program U-15; both Lonza, Basel, Schweiz). On day 2 post transfection ...
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bioRxiv - Immunology 2024Quote: ... and added to the supplied cuvettes and subjected to electroporation using the U-14 program on Amaxa Nucleofector I device (Lonza, Basel, Switzerland). Following electroporation ...
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bioRxiv - Microbiology 2020Quote: ... Homogenate was resuspended with 6 ml per brain of prewarmed complete media (DMEM [Corning]; 10% FBS; 1% Nonessential Amino Acids Mixture 100x, [Biowhittaker Reagents Lonza],1% GlutaMAX supplement 100x [Thermo Fisher Scientific] ...
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bioRxiv - Genomics 2021Quote: ... The EPCs were grown using EPC media (EGM-2MV supplemented with growth factors, ascorbic acid plus 20% Hyclone serum; CC-3202, Lonza and HYC-001-331G ...
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bioRxiv - Cell Biology 2024Quote: ... Differentiation medium consisted of 50 % DMEM with pyruvate and 50 % BEBM supplemented with BEGM singlequots (except amphotericin B, triiodothyronine, and retinoic acid) (Lonza). Medium was supplemented with 100 nM all-trans retinoic acid (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... 190 μL was mixed with 10 μL of 20X SYBER Green I nucleic acid stain (Lonza Walkersville Inc, Walkersville, MD) in a well of a transparent 96-well plate and incubated in the dark for 15 minutes before being placed in the Guava easyCyte HT flow cytometer (488 nm ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative real-time PCR reactions with Taq polymerase prepared following (Pluthero, 1993) and SYBR Green I Nucleic Acid Gel Stain (Lonza) was performed in triplicates using the CFX96 real-time PCR detection system (Bio-Rad ...
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bioRxiv - Genetics 2023Quote: LCLs were treated with 2nM Retinoic acid for 24 hours prior to transfection of firefly and renilla luciferase constructs by Amaxa Nucleofector kit V (Lonza) with the X001 program on a Nucleofector II device ...
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bioRxiv - Plant Biology 2024Quote: ... The nectar was also supplemented with 500 μL of BioWhittaker high non-essential amino acid mix (Lonza, Walkersville, MD, USA), as well as 1g DIFCO BACTO-Peptone (Becton Dickinson ...
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bioRxiv - Cell Biology 2024Quote: ... prepared in tris-acetate (40 mM)/EDTA (10 mM) buffer and 5 μl of GelStar Nucleic Acid Stain 10,000 x (Lonza, 50535). 20 μl per sample resulting from PCR were mixed with Blue/Orange loading buffer loading dye 6x (PROMEGA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PCR products were then loaded and migrated by electrophoresis on a 3% Tris-borate-EDTA (TBE) agarose gel supplemented with GelStar Nucleic Acid Gel Stain (Lonza) for approximately one hour ...
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bioRxiv - Cell Biology 2024Quote: ... confluent 293T-ΔNC cells were treated with a solution of 0.5 g/L trypsin and 0.2 g/L ethylene diaminetetraacetic acid (EDTA) without calcium or magnesium (Lonza; 17-161E) at 37°C to detach and dissociate the cells ...
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bioRxiv - Cell Biology 2024Quote: ... Differentiation medium consisted of 50% DMEM and 50% BEBM supplemented with BEGM SingleQuots (except amphotericin B, triiodothyronine, and retinoic acid; CC-3171 and CC-4175, Lonza). Medium was supplemented with 100 nM all-trans retinoic acid (R2625 ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 11 mM glucose and 25 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) pH 7.4 (Lonza, Basel, Switzerland). The apical (AP ...
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bioRxiv - Bioengineering 2024Quote: ... with L-ascorbic acid-2-phosphate (AA2P) (5mg/ml stock, 10 µl/ml), and β-glycerolphosphate (BGP) (20 mM stock, 20 µl/ml) (Lonza). The cell culture medium was changed every 3 days ...
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bioRxiv - Developmental Biology 2023Quote: ... were cultured in mEC medium (0.1% bovine brain extract (BBE)/10% FBS/1%penicillin-streptomycin/1% Non-Essential Amino Acid (NEAA)/Dulbecco Modified Eagle Medium (DMEM) (DMEM Cat#: 41966-029, Gibco; BBE Cat#: CC-4098, Lonza BioScience). All cells were cultured at 37 °C in a humidified atmosphere with 5% CO2 (HDLECs and HUVECs ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293S GnTI- cells were grown in DMEM with 10% FBS and 1 X nonessential amino acids (NEAAs) from Lonza (Basel, Switzerland). Cells were grown at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... droplets in 6 well plates and treated with retinoic acid for 4 days followed by hepatocyte growth media (Hepatocyte Culture Medium BulletKit (Lonza, CC-3198) supplemented with 10 ng/mL hepatocyte growth factor (PeproTech ...
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bioRxiv - Microbiology 2023Quote: ... and placed into a sterile 2 mm gap cuvette with the appropriate DNA and transfected using an Amaxa Nucleofector II (Lonza, Basel, Switzerland; U-33 program). Parasites were immediately transferred to LIT medium for 24 hours before adding 10 μg/mL puromycin (Invivogen ...
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Malaria parasite HOPS/CORVET complexes are critical for endocytosis and invasion organelles functionbioRxiv - Cell Biology 2024Quote: ... mature schizonts were enriched using a 60% Percoll density gradient and electroporated in transfection buffer containing 50 μg of purified plasmid DNA using an Amaxa Nucleofector II (Lonza, AAD-1001N, program U-033)(Moon et al ...