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Citations for Lonza :
1901 - 1950 of 2029 citations for 4 3 2 Chloroethyl 3 nitrosoureido tetrahydro 2H thiopyran 1 1 dioxide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and 3-fold (SARS-CoV-2 and SARS-CoV) serial dilutions of sample in serum-free EMEM supplemented with 20 µg/ml trypsin (Lonza) for MDCK cells ...
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bioRxiv - Bioengineering 2021Quote: ... SK-N-BE(2) or H460 cells were seeded at densities mentioned above in ultra-low adherent round-bottom 96-well plates (Lonza) for 72 hours ...
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bioRxiv - Immunology 2021Quote: ... Cells were plated at 2 x 106 cells and cultured in RPMI with 10% FCS with low endotoxin levels (Lonza). After 14 d ...
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bioRxiv - Cancer Biology 2022Quote: ... LSECs were counted and 105 cells were seeded in a 48-well plate and cultured in collagenated plates with EGM-2 medium (Lonza) for 3 days ...
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bioRxiv - Molecular Biology 2020Quote: ... pelleted at 350 xg for 10 minutes and resuspended in EBM-2 Basal Medium (CC-3156, Lonzs, Basel, Switzerland) supplemented with Microvascular Endothelial Cell Growth Medium-2 SingleQuots Supplements (CC-4147, Lonza) and 15% fetal bovine serum ...
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bioRxiv - Bioengineering 2020Quote: ... The CD31 negative fraction was then expanded (maximum of ten passages) on uncoated tissue culture plastic substrates supplemented with EGM-2 media (Lonza) and characterized (Fig ...
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bioRxiv - Cancer Biology 2021Quote: ... mixed with 2 µM control or 2 µM target-specific ASO and transferred to nucleocuvettes for nucleofection on a 4D-Nucleofector System (Lonza) using program code “DC-100” ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Successful test compounds from the mf assay were diluted to 10 μM and added to the trans-wells in 6 ml endothelial basal media with supplements (EGM-2 MV; Lonza). Twelve replicates (n = 6 wells ...
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bioRxiv - Microbiology 2021Quote: ... Cells were infected for 30 min with exponential-phase bacteria at a multiplicity of infection (MOI) of 2 and treated with 20 μg/ml of gentamicin (Lonza) for 60 min as described [22] ...
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bioRxiv - Microbiology 2021Quote: Purified SARS-CoV-2 RBD and SED (Spike ectodomain) were diluted in DPBS (without Ca and Mg, Lonza, 17-512F) and coated onto 96-well plates (MaxiSorp - ThermoFisher or High binding - Greiner Bio-one ...
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bioRxiv - Microbiology 2021Quote: ... HeLa (ATCC® CCL-2™), and HaCaT cells (gift from Jonathan Garlick, Tufts University) were grown in Dulbecco’s modified Eagle medium (DMEM; Lonza) containing high glucose ...
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bioRxiv - Cancer Biology 2020Quote: ... murine 3B11 and human HUVECs endothelial cells (all purchased from ATCC) were cultured in RPMI 1640+ 10% Fetal Bovine Serum (FBS) or in endothelial grown media (EGM-2-Lonza).
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bioRxiv - Immunology 2021Quote: ... and soluble anti-CD28 (500 ng/mL) at a density of 2 × 106 cells/mL of X-vivo 15 serum-free medium (Lonza). For iTreg differentiation ...
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bioRxiv - Immunology 2020Quote: ... or media were cultured in 96 well flat bottom plates overnight then PBMC (2×105) from healthy donors were cultured at 37 °C in X-VIVO 15 media (Lonza) with 50-200 U/mL IL-2 (Peprotech) ...
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bioRxiv - Immunology 2021Quote: Human telomerase-immortalized corneal epithelial (hTCEpi) cells (26) were maintained at 37°C/5% CO2 in regular keratinocyte growth medium KGM-2 (Lonza). Prior to treatment ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... pH 7.4 and grown in supplemented keratinocyte growth medium containing 0.15 mM CaCl2 (KBM-2 BulletKit, Lonza Biosciences, Basel, Switzerland) as previously described (38) ...
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bioRxiv - Immunology 2021Quote: ... CD3/CD28 beads were removed 48 hours after stimulation and 5 × 106 CTLs were electroporated with 2 μg plasmid using 4D-Nucleofector (Lonza). Medium was changed 6 hours after nucleofection and transfected cells were used 24-36 hours after electroporation ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were co-transfected with 2 µg of the CRISPR/Cas9 plasmid and 2 µg of ssODN (50 µM) using a Nucleofector II and Amaxa Nucleofector kits (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... at bead-to-cell ratio of 1:1 in human IL-2 medium (X-VIVO 15, serum-free hematopoietic cell medium, with 2mM L-Glutamine and gentamicin (Lonza) supplemented with 5% Human AB serum (Valley Medical) ...
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bioRxiv - Immunology 2023Quote: ... Transfections were performed with an Amaxa Nucleofector device: 2×106 cells were resuspended in 100 μL Nucleofector solution T (Lonza) and added to 2 µg of pcDNA3.1 expression vector encoding full-length IL-2Rβ ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 µg of PX459 V2.0 containing the sgRNA sequence were electroporated into 2×106 DVI2 ESCs (HM1 background) using program A023 of the Amaxa Nucleofector II (Lonza) to ablate endogenous Shh gene expression ...
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bioRxiv - Physiology 2022Quote: HUVEC were grown at 80% confluence (5×103 cells per well) on glass coverslips in a 24-well plate and starved overnight in serum-free medium (EBM-2, Lonza). They were then incubated for 24 hr in EGM2 containing 10μM BrdU ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were washed twice with PBS and 2 × 106 cells were resuspended in 100 μl of Solution V (Lonza) and then mixed with 5 μg purified sgRNA expression vector or the empty vector as a negative control before nucleofection using program Q-001 of a Nucleofector™ 2b (Lonza) ...
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bioRxiv - Bioengineering 2024Quote: ... HSPCs (2×105 cells/condition) were transfected with RNP complexes using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza) and the CA137 program (Nucleofector 4D ...
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bioRxiv - Bioengineering 2024Quote: ... NHDF cells were cultured in FBM fibroblast basal media (supplemented with FGM-2 SingleQuot supplements: insulin, hEGF-B, GA-1000, and FBS) (Lonza). NHEK cells were expanded in KBM gold basal medium (KGM gold supplemented with Lonza’s SingleQuot supplements ...
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bioRxiv - Cancer Biology 2024Quote: ... were isolated from 8 to 16-week-old Rptorfl/fl or Tsc2fl/fl mice and maintained in EGM-2 medium (Lonza), as previously described71–75 ...
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bioRxiv - Cancer Biology 2024Quote: ... The OCI-LY3 cell line was cultured in IMDM with 25 mM HEPES and 2 mM L-glutamine (Lonza; Switzerland), supplemented with P/S ...
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bioRxiv - Cell Biology 2024Quote: ... Large scale D.Mel-2 cultures were prepared by diluting cells to a density of 106 cells/mL in Insect Xpress culture medium (Lonza) in Erlenmeyer flasks ...
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bioRxiv - Cancer Biology 2024Quote: ... by flushing one femur with PBS 2% FBS followed by erythrocyte elimination with ACK Lysing Buffer (10-548E, BioWhittaker, Lonza) (Figure 4BC) ...
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bioRxiv - Cell Biology 2023Quote: ... JAWS transfection was achieved using 20 μg DNA and 2 x 106 million cells per 100 μl nucleofection solution from the Amaxa Nucleofector Mouse Dendritic Cell kit (Lonza), and the Y-001 program ...
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bioRxiv - Plant Biology 2024Quote: ... The fixed cells were washed three times with the same buffer and then embedded in 2 % low-melting-temperature agarose (SeaPlaque, Lonza). The embedded samples were post-fixed with 4 % potassium permanganate at 4 °C for 2 h ...
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bioRxiv - Molecular Biology 2024Quote: HUVEC were grown at 80% confluence (5×103 cells per well) on glass coverslips in a 24-well plate and starved overnight in serum-free medium (EBM-2, Lonza). They were then incubated for 24 hr in EGM2 containing 10µM BrdU ...
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bioRxiv - Immunology 2023Quote: ... and cultured in 96-well culture plates (2×103 cells/well) with serum or EP-stimulated MDMs supernatants in X-VIVO 15 medium (Lonza) with 10 U/mL of IL-2 (PeproTech ...
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bioRxiv - Microbiology 2023Quote: ... Stable cell lines were generated by nucleofecting 2 µg of pBud-PCID2-Flag plasmid or empty pBud as control using Amaxa Nucleofector (Lonza) and Nucleofector Kit R (Lonza ...
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bioRxiv - Molecular Biology 2023Quote: ... spanning the whole SARS-CoV-2 genome was transfected into 2×106 HEK293T cells using the SF Cell Line 4D-Nucleofector-X Kit (Cat# V4XC-2012, Lonza) and the 4D-Nucleofector X Unit (Lonza ...
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bioRxiv - Immunology 2023Quote: ... Mixtures of complexed sgRNA and Cas9 (0.3 nmol synthetic sgRNA + 62 µmol Cas9 nuclease) and 2-10×106 enriched murine CD8+ T cells were suspended in 25ul of P3 electroporation buffer (Lonza) and electroporated using the Lonza 4D Nucleofector (pulse code DN100) ...
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bioRxiv - Bioengineering 2022Quote: ... 30 µl was mixed with 6 µl 6X gel loading dye and run on a 50 ml gel containing 2% SeaKem Agarose (Lonza), 1x Tris-Acetate-EDTA (Boston BioProducts) ...
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bioRxiv - Bioengineering 2022Quote: ... Bone marrow mononuclear cells were plated in endothelial growth medium 2 + 10% fetal bovine serum (FBS) (EGM-2MV, Lonza, Switzerland) on fibronectin coated plates and cultured at 37°C in 5% CO2 incubators ...
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bioRxiv - Cell Biology 2022Quote: ... Three slices were placed in one well of a 12-well plate and incubated with 2 mL DMEM (Lonza, #42430) with 0.6% amphotericin B ...
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bioRxiv - Systems Biology 2022Quote: ... The red blood cell lysis was performed by resuspending the pellet in 2 ml sterile ACK (Ammonium-Chloride-Potassium) lysis buffer (ACK Lysing Buffer, Lonza) and incubating on ice for 5 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... washed three times in PBS and cultured on collagen-coated 6-well plates in endothelial growth medium (EGM-2, Lonza) composed of endothelial basal medium supplemented with 2% fetal bovine serum ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary MEC were cultured in endothelial growth media (EGM) containing 5% FBS with growth factors (EBM-2; Lonza, Basel, Switzerland) at 37°C in 5% CO2 ...
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bioRxiv - Genomics 2022Quote: ... cells (from two donors) were grown to 80% to 90% confluence in endothelial basal medium 2-MV with supplements (EBM; Lonza) and 5% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Five million BJAB or OCI-LY1 cells were transfected with 2 μg plasmid DNA using the SF Cell Line 4D-Nucleofector X Kit L (Lonza) and the AMAXA Nucleofector biosystem (BJAB ...
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bioRxiv - Developmental Biology 2024Quote: ... we proceeded with 50% Basal media (+10uh/ml HGF) and 50% SkGM™-2 Skeletal Muscle Cell Growth Medium (Lonza). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... A total of 2 × 104 cells were seeded in each well with 150 μl EC specific media (Lonza, CC-3202). TIFF images of capillary-like networks were captured using a Zeiss Axio Image M2 microscope equipped with a digital camera at 4 ...
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bioRxiv - Molecular Biology 2024Quote: ... resuspended in RDM to OD600 of ≈0.003 and several 1-µl drops of the diluted cell suspension was placed on an agarose pad prepared with RDM and 2% agarose (SeaPlaque GTG Agarose, Lonza). The agarose pad was surrounded with a gene frame (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... siRNAs were added to 2 µM and electroporation was performed using the program U-001 on the 3D-Nucleofector (Lonza). The siRNAs targeting Clever-1 ...
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bioRxiv - Microbiology 2024Quote: ... and 2.5µg of each of the sgRNAs were incubated together for at least 10 minutes at room temperature before nucleofection of iPSC cells occurred using a Human Stem Cell Nucleofector Kit 2 (Lonza). Cells were then plated at 25 cell/cm2 on 2x Cultrex-coated plates in mTeSR-Plus media with 1× CloneR supplement (STEMCELL Technologies) ...
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bioRxiv - Immunology 2024Quote: ... 1-2*106 T-ALL cells were resuspended in 100uL P3 nucleofector solution (82 uL nucleofection solution + 18 uL supplement; Lonza P3 Primary Cell 4D-NucleofectorTM X Kit) ...