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Citations for Lonza :
1801 - 1850 of 2572 citations for PGFM ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the transfected cells were co-cultured with BHK-21 cells (ATCC CCL-10) transfected with VSV-G using the SE cell Line 4D-Nucleofector X Kit L (Lonza) in DMEM without glutamine (Life Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... 200,000 cells were electroporated with 1000 ng adRNA plasmid or 48 pmol of IVT RNA using the Amaxa SF cell Line 4D-Nucleofector X kit (Lonza) as per the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... The cells were used up to passage number 12 and were tested for mycoplasma contamination (MycoAlertTM Mycoplasma Detection Kit, Lonza) at first and last passage number ...
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bioRxiv - Neuroscience 2021Quote: ... The efficacy of siRNA targeting Kdm6a was assessed by electroporation of neurons using a 4D-Nucleofector X Unit and the corresponding P3 Primary Cell nucleofection kit (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were maintained in 37 °C and 5% CO2 incubators and routinely tested negative for mycoplasma contamination using the MycoAlert Kit (Lonza).
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bioRxiv - Cancer Biology 2020Quote: ... Their identity was verified by using STR profiling (Eurofins) and lack of Mycoplasma infection was confirmed by Mycoalert Mycoplasma Detection Kit (Lonza). Cell lines were cultured in Dulbecco’s modified eagle’s medium (DMEM):F12-HAM (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... were cultured according to manufactureŕs instructions in a humidified incubator with 5% CO2 at 37 °C and routinely checked and tested negative for mycoplasma contamination using MycoAlertPlusTM Mycoplasma Detection Kit (Lonza) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The RNP complex and 1.5 μg of a linearized homology-directed repair template plasmid were transfected into 2×105 – 5×105 nocodazole-arrested mitotic HeLa A12 cells using a Nucleofector and the associated Cell Line Kit R (Lonza) following manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... All of the cell lines have been periodically subjected to re-confirmation by Short Tandem Repeat (STR) profiling by ATCC and mycoplasma testing by MycoAlertTM PLUS mycoplasma detection Kit (Lonza). A375 ...
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bioRxiv - Microbiology 2021Quote: ... Raw264.7 Ifit2 CRISPR cells were generated by electroporating low passage Raw264.7 cells with Ifit2 pSBtet-puro-Cas9-U6 using Amaxa Nucleofector II and Amaxa Cell Line Nucleofector Kit V (Lonza). Cells were selected with puromycin 3 days post-nucleofection ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were maintained at 37 °C and 5% CO2 and were frequently tested for mycoplasma contamination using MycoAlert Mycoplasma Detection Kit (Lonza). Cells were plated on 25-mm diameter coverslips and transfected using Fugene6 transfection reagent (Roche ...
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bioRxiv - Neuroscience 2020Quote: iPSNs were transfected using the Lonza nucleofection system (program DC104) and a Lonza P3 Primary Cell 4D Nucleofector Kit (Lonza). POM121 ...
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bioRxiv - Microbiology 2021Quote: ... Both resuspensions were mixed in a 100 μl cuvette (P3 Primary cells 4D-Nucleofector X kit L, V4XP-3024, Lonza). The programme FI-158 was used for electroporation ...
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bioRxiv - Molecular Biology 2020Quote: ... at Day 3-4 of phase I culture were electroporated using P3 Primary Cell 4D NucleofectorTM X Kit (from Lonza) with program DZ100 (Bak et al. ...
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bioRxiv - Microbiology 2020Quote: ... All cells used in this study were cultured in a humidified incubator at 37°C with 5% CO2 and routinely tested and certified as mycoplasma-free using the MycoAlert kit (Lonza). STR analysis (Idexx ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were typically maintained in culture for 1-2 months before thawing of a new batch and mycoplasma screening was performed routinely using the MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cancer Biology 2020Quote: ... All cells were confirmed to be mycoplasma-free by MycoAlert Mycoplasma Detection Kit (Lonza, Morristown, NJ USA; Cat #: LT07-118). Cells were steroid-starved in phenol red-free medium containing 10% charcoal stripped-FBS (5% CS-FBS for C4-2 cells ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines were maintained and passaged according to ATCC recommended procedures and regularly tested for mycoplasma using MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cancer Biology 2020Quote: ... All cells were grown at 37°C in a humidified atmosphere with 5% CO2 and tested for lack of mycoplasma contamination (MycoAlert Mycoplasma Detection Kit, Lonza) prior to experiments (initiated within the initial 1-4 passages).
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bioRxiv - Cancer Biology 2021Quote: ... Both cell lines were authenticated by amplification and Sanger sequencing of the BRCA1 locus harboring the original and secondary mutations and regularly tested for Mycoplasma contamination using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza). The two cell lines were maintained in Ham’s F-12 Nutrient Mixture medium with 5% (vol/vol ...
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bioRxiv - Cell Biology 2022Quote: ... were nucleofected with either PGK-mO-Dam or PGK-mO-Dam-Zfp266 plasmids using the P4 Primary Cell 4D-Nucleofector X Kit (Lonza). 5 replicates were performed in total ...
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bioRxiv - Cancer Biology 2022Quote: Cell lines were cultured according to standard aseptic mammalian tissue culture protocols in 5% CO2 at 37 °C with regular testing for mycoplasma contamination using the MycoAlertTM Mycoplasma Detection Kit (Lonza). HCT116 ...
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bioRxiv - Microbiology 2022Quote: ... All cell lines were regularly tested to check they were free of mycoplasma contamination using a commercially available system (MycoAlert Mycoplasma Detection kit; Lonza). HCV replication experiments were conducted using the highly permissive human hepatoma cell line Huh7 High Passage (28 ...
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bioRxiv - Neuroscience 2022Quote: ... 3 x 106 NSCs were mixed with 5 µg of corresponding DNA with the Amaxa P4 Primary Cell 4D-Nucleofector X Kit S (Lonza) and pulse was delivered using the CA137 programme in a 4D-Nucleofector X Unit (Lonza) ...
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bioRxiv - Cell Biology 2022Quote: ... All cell lines were tested to be negative for mycoplasma every 2–3 months using the MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cancer Biology 2022Quote: ... cells were transfected with 5μg of selected plasmid (control, empty vector (EV) or containing guide RNAs using Amaxa Cell Line Nucleofector kit (Lonza Bioscience) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 2×105 NSCs for essential gene expression were nucleofected with 750 ng each of the above-mentioned plasmids using SG Cell Line 4DNucleofector X Kit (Lonza), DN-100 program ...
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bioRxiv - Cell Biology 2022Quote: A GFP sgRNA vector was delivered into Cas9 and GFP expressing NSCs using nucleofection with the SG Cell Line 4DNucleofector X Kit (Lonza) as per manufacturer’s instructions51 ...
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bioRxiv - Bioengineering 2022Quote: ... and 1 μg of a plasmid encoding a hyperactive piggyBac transposase (hyPBase) 39 were co-electroporated into Im-HUVEC using the P5 Primary Cell Kit (Lonza) and a 4D-nucleofector (Lonza) ...
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bioRxiv - Bioengineering 2022Quote: ... All cells were routinely verified as free of mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza Group, Basel, Switzerland). MDA-MB-231 cells were first transduced with lentivirus (MOI=5 ...
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bioRxiv - Cell Biology 2022Quote: ... Expression of eGFP-Centrin1 was achieved by electroporating 4.106 B lymphoma cells with 4 μg of plasmid using the Amaxa Cell Line Nucleofector Kit R (T-016 program, Lonza). Cells were cultured in CLICK medium for 5 to 16 h before imaging.
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bioRxiv - Immunology 2022Quote: ... Twenty μl of cells in buffer were transferred to each well of a 16-well Nucleofection cuvette strip (4D-Nucleofector X kit S; Lonza). Three μl of RNP were gently dispensed to each well ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines in culture were screened biweekly for mycoplasma contamination using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza, #LT07-703).
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bioRxiv - Cell Biology 2022Quote: ... we used Cas9-RNP and the Lonza 4D-Nucleofector System together with the P4 Primary Cell 4D-Nucleofector X Kit L (Lonza) to transfect and create Ndrg2-KO DCs ...
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bioRxiv - Cell Biology 2022Quote: ... For nucleofection we used the 4D-Nucleofector System and P3 Primary Cell 4D Nucleofector X Kit L (Lonza, Basel, Switzerland). We optimized the RNP electroporation for DCs by using multiple-guide mixtures and different number of cells in a 100uL total volume ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were introduced into HCT116 and MDCK cells using nucleofection with the 384-well HT Nucleofector and the SE cell line kit (Lonza) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... All tumor cell lines used in the in vivo experiments were routinely tested for mycoplasma contamination using Mycoalert plus mycoplasma detection kit (Lonza). Randomization of animals was not used in experiments and no blinding was done for the animal experiments.
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bioRxiv - Cancer Biology 2022Quote: ... siControl and siIRAK4 were purchased from Horizon Discovery and transfected into human AML samples using Amaxa Nucleofector Kit T (Lonza) (program number G-016 ...
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bioRxiv - Neuroscience 2021Quote: ... the cell suspension was transfected with a Homer 1c::TdimerDsRed plasmid (Bats et al., 2007) by electroporation using the Amaxa Nucleofector 2b device and Amaxa mouse neuron nucleofector kit (Lonza). Neurons were then plated on poly-L-lysine-coated glass coverslips and grown at 37°C in a humidified 5% CO2-containing atmosphere in BME supplemented with KCl (25 mM final concentration) ...
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bioRxiv - Cell Biology 2021Quote: ... Both endothelial cell lines (HUVEC, hPAEC) were grown in EBM-2 media with EGM-2 bullet kit (purchased from Lonza). No additional serum was used for cell culture ...
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bioRxiv - Neuroscience 2020Quote: ... control and C9orf72 iPSNs were nucleofected using the Lonza nucleofection system (program DC104) and a Lonza P3 Primary Cell 4D Nucleofector Kit (Lonza) as described above ...
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bioRxiv - Cell Biology 2021Quote: ... or mutant) plasmids (4µg) were introduced to 4×106 dissociated neurons using Amaxa rat nucleofector kit (Lonza Bioscience, VPG-1003). Amaxa-nucleofected neurons plated at 500,000 cells per 35mm poly-D-lysine-coated glass bottom dish (WillCo Wells ...
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bioRxiv - Microbiology 2020Quote: ... and maintained in D10 media or endothelial growth medium 2 (EGM-2) using the EGM-2 bullet kit from Lonza, respectively ...
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bioRxiv - Biophysics 2022Quote: ... CD146 positive cells were retained in the column and flushed with 5 mL warmed EGM-2 growth medium supplemented with EGM-2 bullet kit (Lonza) into a separate tube ...
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bioRxiv - Genetics 2022Quote: ... Libraries were electroporated into K562 cells in 100ul volumes using the Amaxa system (Program X-001, Neucleofactor kit V, Lonza). We performed 6 independent replicates with each replicate consisting of ∼2×108 cells ...
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bioRxiv - Immunology 2022Quote: ... 2 million B cells were then washed with PBS and resuspended in 15 uL resuspension buffer (4D-Nuclcofector X Kit S, #V4XP-4032; Lonza). Cells in resuspension buffer (12.5ul ...
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bioRxiv - Immunology 2022Quote: ... Knockdown of Vps4a/b was performed using Amaxa Nucleofector II and the Mouse T-Cell Nucleofector Kit (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... All cells were grown at 37°C and 5% CO2 in a humidified incubator and tested negative for mycoplasma using the MycoAlert Mycoplasma Detection Kit (LT07-318, Lonza).
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bioRxiv - Biochemistry 2022Quote: Generation of overexpressing POLIC variant cell lines was previously described.10 10 μg pLEW79IA-MHTAP was NotI linearized and transfected into 29-13 procyclic cells by electroporation.8 7.5 μg pLew100-PTPPuro plasmids encoding full length POLIB and POLID variants were NotI linearized and transfected into 29-13 cells via Amaxa Nucleofection (Parasite Kit, Lonza). Following selection with 1 μg/mL puromycin for pLew100 based constructs and 2.5 μg/mL phleomycin for cells transfected with pLEW79IA-MHTAP ...
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bioRxiv - Physiology 2022Quote: ... All human iPSC lines were negative for mycoplasma contamination as tested using the Mycoalert Mycoplasma testing kits (LT07-318, Lonza) and no karyotype abnormalities were found (KaryoStat+ ...