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Citations for Lonza :
1601 - 1644 of 1644 citations for Mouse Plectin PLEC ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... and the gRNA vector PX330-EN1082 using nucleofection with the Amaxa 4D-Nucleofector X-Unit and the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, V4XP-3024 KT) as described above ...
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bioRxiv - Molecular Biology 2022Quote: ... was transfected with the targeting vector pUC19-ITR-NeoR-ITR-3xCTCF-LacO and the gRNA vector pX330-chr15_LacO_gRNA/Cas9 using nucleofection with the Amaxa 4D-Nucleofector X-Unit and the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, V4XP-3024 KT) as described for the Tir1 integration ...
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bioRxiv - Molecular Biology 2022Quote: ... were transfected with the targeting vector pMK-3xCTCF-TetO-Rox-PuroR-Rox and the gRNA vector PX459-chr15_gRNA/Cas9 using nucleofection with the Amaxa 4D-Nucleofector X-Unit and the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, V4XP-3024 KT). 2×106 cells were nucleofected with 1 μg TetO targeting vector and 1 µg of PX459-ch15_gRNA/Cas9 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell pellets of 1E6 cells were resuspended with 20ul supplemented nucleofector solution of the P3 Primary Cell 4D-nucleofector® X Kit S (Lonza, V4XP-3032) and 2ug plasmid (1ug/ul) ...
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bioRxiv - Immunology 2022Quote: ... Activated cells were nuclofected with 100 µM AhR or non-targeting (NT1) siRNA (ON-TARGETplus SMART pool, Dharmacon) using the Amaxa Human T cell Nucleofector Kit (Lonza, Walkersville, MD, USA), according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... we transfected HeLa cells with SNAP-tagged CENP-A (generous gift from Dan Foltz) in combination with either empty vector or GFP-CENP- C using the Amaxa Nucleofector kit R (Lonza Bioscience, Walkersville, MD) per instructions.
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: SQD9 cells were transfected with PX459 using Nucleofector™ 2b Device and Cell Line Nucleofector™ Kit L (Lonza, #AAB-1001, #VACA-1005). Cas9-expressing cells were selected with 0.625 µg/mL puromycin (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... into control and disease related iPSC lines in Amaxa® 4D- Nucleofector® using P4 Primary Cell 4D-Nucleofector® X Kit (Lonza) with the CM133 program ...
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bioRxiv - Cell Biology 2023Quote: ... Next 1 × 106 cells resuspended in 93 μL of P3 Primary Cell Nucleofector Solution (P3 Primary Cell 4D-Nucleofector X Kit, Lonza, Catalog #: V4XP-3024) were mixed with 7 μL of RNP and 12 μg of single-stranded oligodeoxynucleotide (ssODN ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cell line identities were confirmed by short tandem repeat profiling.77 Cell cultures were routinely tested for Mycoplasma contamination using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza Bioscience, cat # LT07) according to manufacturer protocol ...
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bioRxiv - Bioengineering 2023Quote: ... PiggyBac transposon constructs and RNA encoding transposase were introduced into T cells by electroporation using a Lonza 4D-Nucleovector instrument and the P3 Primary Cell Kit (Lonza, Cat. # V4XP-3032). Electroporated T cells were immediately seeded into 24-well G-Rex plates (Wilson Wolf ...
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bioRxiv - Immunology 2023Quote: ... were incubated with sgRNA and CAS9 protein complex and electroporation was done using P3 Primary Cell 4D-NucleofectorTM X Kit L (Lonza, cat # V4XP-3024) and Lonza 4D nucleofector system ...
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bioRxiv - Cancer Biology 2023Quote: Electroporation of multiplex CRISPR-Cas9 pX330 plasmids into normal bronchial epithelial cells was achieved using the P3 Primary Cell 4D-Nucleofector® X Kit L (Lonza, V4XP-3024) and 4D-Nucleofector™ X Unit (Lonza) ...
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bioRxiv - Cancer Biology 2023Quote: ... The RNP complex was mixed with 8 x 10e5 GBM1 cells resuspended in 100 μl of SG solution from the SG Cell Line 4D-NucleofectorTM X Kit (Lonza, Cat# V4XC-3024). This entire mix was then placed in a NucleocuvetteTM and was nucleofected using a Lonza Amaxa 4D-Nucleofector TM and the EN138 nucleofection programme ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated at 37°C in a humidified incubator with 5% CO2 and frequently tested for mycoplasma contamination using MycoAlert™ detection kit (Lonza, LT07-118).
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bioRxiv - Cancer Biology 2023Quote: ... Plasmids expressing ETS1 sgRNAs (see Supplemental Table S1) were co-electroporated into the dCas9-VP64-expressing cells using the AmaxaTM Cell Line Nucleofector™ Kit R (Lonza, VCA-1001) and further subjected to puromycin selection and single-cell cloning ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured at 37°C with 5% CO2 and were regularly tested for mycoplasma contamination using the MycoAlert® PLUS Mycoplasma Detection Kit (Lonza, LT07-703), and authenticated by Microsynth ...
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bioRxiv - Immunology 2024Quote: ... MC38 Pdl1 KO cells were generated through CRISPR Cas9-gRNA RNP-directed deletion by using a Lonza 4D-Nucleofector and a SF Cell Line 4D-Nucleofector X Kit S (Lonza, cat. V4XC-2032). In brief ...
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bioRxiv - Immunology 2024Quote: ... The nucleofection was conducted using a Lonza 4D-Nucleofector and a P3 Primary Cell 4D-Nucleofector X Kit S (Lonza, cat. V4XP-3032) with the same protocol described earlier ...
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bioRxiv - Neuroscience 2024Quote: ... sgRNA plasmids validated by Sanger sequencing were transfected into SH-SY5Y cells using nucleofection (SF Cell Line 4D-Nucelofector X kit, Lonza, Germany, V4XC-2012) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... 6 µg of total plasmid DNA (2 µg for each of the three episomal vectors) were electroporated into 5×10^5 fibroblasts with the Amaxaμ Human Dermal Fibroblast Nucleofectorμ Kit (Lonza, Basel, Switzerland; VDP-1001). The fibroblasts were further cultured for 6 days to allow them to recover ...
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bioRxiv - Microbiology 2021Quote: ... electroporated with 10 μg of linearised plasmid DNA or PCR product using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (Lonza, Switzerland, program X-001).
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bioRxiv - Cell Biology 2021Quote: MLIV patient fibroblasts were grown for 2-3 days until 80-90% confluent at 37°C and prepared for electroporation with approximately 50nM of ON-TARGETplus siRNA SMARTpool reagents from Horizon Cell Solutions (Dharmacon) according to manufacturer instructions using the Amaxa P2 Primary Cell 4D-Nucleofector X Kit (Lonza, Cat. No. V4XP-2032) with nucleocuvette strips in a Lonza 4D-Nucleofector X Unit ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were also transfected with electroporation using Amaxa™ Nucleofector Kit R (Cat.no.VCA-100) and the Nucleofector™II device (program W-001, Lonza Group AG, Cologne, Germany) as suggested by the manufacturer.
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bioRxiv - Developmental Biology 2022Quote: Generation of Foxa2 knockout cell lines was performed by electroporating two different guides cloned into the pX458 vector (Ran et al., 2013) with the AMAXA nucleofector kit (Lonza Cat no. VPH-1001). Cells were sorted for GFP as single cells one day after electroporation ...
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bioRxiv - Immunology 2020Quote: ... 1.3×10^5 C25cl48 cells/reaction were electroporated with a ribonucleoprotein consisting of 125 pmol TrueCut Cas9 Protein v2 (A36498) + 150 pmol gRNA + 120 pmol ssDNA using P5 Primary Cell 4D-Nucleofector X Kit S (Lonza, Walkersville, MD, V4XP-5032) in a 16-well Nucleocuvette Strip and the Amaxa 4D-Nucleofector X Unit (Lonza ...
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bioRxiv - Cancer Biology 2019Quote: ... and 3-4 hours later were transfected with 2 μg of pBabe KRAS WT and pBabe KRAS G12D or pBabe KRAS Q61H plasmid DNA by electroporation using the Amaxa® Human T Cell Nucleofector® Kit (Lonza, Basel, Switzerland) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: The murine myoblast cell line C2C12 was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and was tested for mycoplasma (MycoAlert™ Mycoplasma Detection Kit, Lonza AG, Basel, Switzerland). Cells were cultured in monolayer at 37 °C in a 5% CO2-containing humidified atmosphere in complete growth medium (GM) ...
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bioRxiv - Cancer Biology 2023Quote: CD4 SMARTA T cells were isolated from naïve mice and were washed and resuspended in 20 μl buffer P3 (P3 Primary Cell 4D-Nucleofector X Kit S, Lonza, cat. no. V4XP-3032). Between 105 to 107 cells were used per nucleofection ...
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bioRxiv - Cell Biology 2023Quote: ... The cell line was authenticated through short tandem repeat analysis and tested for mycoplasma contamination every 10 passages using the MycoAlert Mycoplasma Detection Kit (Lonza Group Ltd, Basel, Switzerland). PDGFR-BiFC and myr-Venus stable cells were cultured in RPMI growth medium [RPMI 1640 (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... 4−106 cells were pelleted at 300xg and then resuspended in 100 μL prewarmed nucleofector solution (Human Stem Cell Kit 1, Lonza, catalog no. VPH-5012); 4 μg of NGN2 plasmid and 1.5 μg each of 1R and 1L plasmids were added directly to the resuspended cells ...
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bioRxiv - Cancer Biology 2023Quote: All cell lines were cultured at 37°C and 5% CO2 and tested for mycoplasma contamination using a MycoAlert® mycoplasma detection kit (Lonza, cat: LT07-218). Only mycoplasma-negative cells were used.
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bioRxiv - Synthetic Biology 2023Quote: ... CD22-deleted clones modified to express PD-L1 (LZRS PD-L1) or CD200 (PB-EF-1α-intron CD200, pCMV-hyPBase, SF buffer kit V4XC-2032, Lonza 4D Nucleofector, code DN100). Transposition reactions used 1-2 μg of vector DNA and 0.5-1.0 μg of pCMV-hyPBase transposase DNA ...
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bioRxiv - Developmental Biology 2023Quote: ... mESCs were transfected with the pre-assembled fRNP pool by electroporation (program: OP315 or CD112) using an SE Cell line 4D-Nucleofector™ X kit (Lonza, Catalog#: V4XC-1024). The electroporated cells were plated in Nunc Glass Bottom Dishes (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... to aid complete removal of PBS before resuspension in P3 Nucleofection Solution (room temperature; P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza; cat. no., V4XP-3032). A total of 20 μl solution was used to resuspend 80,000 cells ...
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bioRxiv - Genomics 2023Quote: ... All iPSCs were karyotypically validated via g-banded karyotyping (WiCell) and are regularly screened for mycoplasm via MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza Cat # 75860-362). Two major approaches to generating neurons in a dish are based on overexpression of proneural factors combined with chemicals from iPSCs differentiation or directly converted from fibroblasts ...
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bioRxiv - Neuroscience 2021Quote: ... LUHMES cells were electroporated and transfected with 2 μg of plasmid using the Amaxa™ Basic Primary Neurons Nucleofector™ Kit and protocol (Lonza, cat. # VPI-1003). Transfected cells were selected by flow-sorting for DAPI negative ...
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bioRxiv - Cell Biology 2019Quote: Nucleofection of HeLa cells was performed using a Lonza 4D-Nucleofector™ device and Amaxa™ SE Cell Line 4D-Nucleofector™ X Kit (Lonza - V4XC-1024), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... The cells were cultured in a humidified chamber at 37 °C under 5% CO2 and were routinely tested for mycoplasma contamination using MycoAlert plus detection kit (Lonza, Basel, Switzerland; cat. LT07-701).
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bioRxiv - Genomics 2022Quote: ... 1.0µg/µl SpCas9-sgRNA-PGK-Venus construct and 1µg/µl donor construct were nucleofected into ∼3×106 Tir1 mESCs using the Amaxa™ 4D-Nucleofector and the P3 Primary Cell 4D-Nucleofector™ X Kit (Lonza, Cat. V4XP-3024) following the manufacture’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... BU3 NG iPSCs were pretreated with ROCK Inhibitor (Y-27632) for three hours prior to nucleofection using the Lonza P3 Primary Cell 4DNucleofector™ X Kit (Lonza, cat. no. V4XP-3024) and replated for puromycin resistance screening ...
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bioRxiv - Immunology 2024Quote: 1-1.5 x106 triple reporter TCR-/- Jurkat cells were mixed with 2μg of pLVM containing gene of interest and electroporated using pulse code CL-120 according to manufacturer’s protocol (Lonza #V4XC-1024, SE Cell line kit). After 48-72hrs ...
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bioRxiv - Cancer Biology 2024Quote: ... hTERT-HME1 (CRL-4010) were acquired from ATCC and cultured in Mammary Epithelial Cell Growth Medium Bullet Kit from Lonza (Bend, OR, Catalog #: CC-3150).
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bioRxiv - Biochemistry 2022Quote: ... each insert was transferred in a new 24-well plate filled with 500 μl and 300 μl of fresh BEGM (supplemented with Lonza Bullet Kit + 20mM of HEPES buffer) respectively at the basal and apical compartments ...