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Citations for Lonza :
1551 - 1600 of 1973 citations for Acyl protein thioesterase 2 LYPLA2 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: Cryopreserved PHHs were purchased from vendors permitted to sell products derived from human organs procured in the United States of America by federally designated Organ Procurement Organizations (BioIVT, Baltimore, MD, and Lonza, Walkersville, MD). PHH lots included HUM4055A (54-year-old female Caucasian ...
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bioRxiv - Neuroscience 2020Quote: ... hESCs human cortical neurons were nucleofected with shRNAs constructs against ASH1L using the X-unit of the Amaxa nucleofector unit (Lonza, catalog # AAF1002X). Human neurons between days 30 to 32 of neuronal induction were single cell dissociated using single cell dissociation reagent (stem cell technologies CT catalog # 07174 ...
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bioRxiv - Cell Biology 2021Quote: ... OC activity was measured by releasing the europium conjugated human Type I collagen-coated on the bottom of the OsteoLyse™ Assay Kit (Lonza Biosciences) at each time point ...
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bioRxiv - Cancer Biology 2021Quote: ... Immortalized human bronchial epithelial BEAS-2B cells were purchased from ATCC and cultured in BEGM bronchial epithelial cell growth medium (Lonza #CC-4175). All the cells were grown at 37°C with 5% CO2.
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bioRxiv - Developmental Biology 2022Quote: ... Cells were electroporated with 10 µg lineage recorder DNA and 1 µg Sleeping Beauty transposase in 100 µl Human Stem Cell nucleofection buffer (Lonza, VVPH-5022) using the B-016 program of the 4D-Nucleofector (Lonza ...
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bioRxiv - Physiology 2020Quote: Growth media used for the expansion of human muscle-derived cell populations consisted of Hams F-10 nutrient mix (Lonza, Basel, Switzerland) with added L-glutamine (2.5 mM) ...
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bioRxiv - Immunology 2021Quote: Primary BEAS-2B (ATCC® CRL-9609TM) (normal human airway epithelial cells) as well as all the basal media and growth supplements were obtained from Lonza (Walkersville, MD). Cells were cultivated according to the instructions of the manufacturer on plastic dishes or flasks (BD Bioscience ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 mmol/L HEPES (PAA Laboratory) and 1 ng/mL human basic fibroblast growth factor (bFGF) (Sigma-Aldrich) (Lonza, Walkersville, MD, USA). The generation number of ECs is maintained between 30-40 generations ...
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bioRxiv - Immunology 2019Quote: Primary human normal airway tracheobronchial epithelial cells from de-identified donors (NHBE) (sex: male and female) were provided by Lonza (Walkersville, MD) and grown in BEM media supplemented with the BEGM bullet kit (Lonza) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... plasmids pSpCas9n(BB)-ZEB2-Guide-A &-B (0.5 µg of each plasmid) were electroporated into 1×106 H9 cells using the Human Stem Cell Nucleofector Kit 1 (Lonza, VPH-5012). Following electroporation ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µg of sgRNA and 5 µg of ssDNA donor template (Table S1) using Human Stem Cell NucleofectorTM kit 1 (Lonza, VVPH-5012) according to the manufactures protocol and cells replated ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with pcDNA3.1 empty vector or pcDNA3.1 containing the human coding sequence of the VGLL2-NCOA2 fusion using the AMAXA cell line Nucleofection kit V (Lonza #VCA-1003;) and were selected in growth media containing 1μg/ml concentration of G418 (ThermoFisher #10131035) ...
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bioRxiv - Microbiology 2023Quote: ... BEAS-2B cells (human bronchial epithelial; CRL-9609ATCC) cells were grown in bronchial epithelial cell growth basal media (BEBM; Lonza/CC-3170). LLC-MK-2 cells (rhesus monkey kidney ...
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bioRxiv - Microbiology 2024Quote: ... 5 million parasites were electroporated with 5-10ug of digested plasmid with an AMAXA Nucleofector II using X-001 in Human T-cell Nucleofector Solution (Lonza VPA-1002).
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bioRxiv - Molecular Biology 2023Quote: Induced pluripotent stem cells were transfected with a total of 5 μg target Cas9/gRNA plasmids with or without 1 μg repair templates using Human Stem Cell Nucleofector Kit (LONZA, Basel, Switzerland) and Nucleofector 2b device (LONZA ...
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bioRxiv - Cell Biology 2023Quote: ... The linearized construct and the purified schizonts were mixed with Nucleofector solution from an Amaxa human T cell Nucleofector Kit and electroporated using the Amaxa Nucleofector II device (Lonza, Köln, Germany). Directly after transfection 50 µl RPMI-1640 complete was added to the transfection reaction followed by intravenous injection into one SWISS mouse ...
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bioRxiv - Immunology 2023Quote: ... 10% FBS heat-inactivated and 1% Penicillin/Streptomycin. Human Small Airway Epithelial Cells (SAECs, Cat. CC-2547) and the corresponding medium were commercially obtained by Lonza (NJ, USA). Human Pulmonary Microvascular Endothelial Cells (HPMECs ...
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bioRxiv - Cell Biology 2023Quote: ... MCF10A cells (female human mammary epithelial cells) were purchased from ATCC (CRL-10317) and cultured as recommended by ATCC in MEGM (Lonza CC-3150) supplemented with bovine pituitary extract ...
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bioRxiv - Neuroscience 2020Quote: ... two pairs of sgRNA vectors and Cas9 nickase plasmid were transduced into the AD hPSCs by using Human Stem Cell Nucleofector® Kit 1 (Lonza VPH-5012). Three days after electroporation ...
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bioRxiv - Neuroscience 2021Quote: ... 1 × 106 cells were resuspended in 100 μl of the nucleofection hESC solution 1 (Human Stem Cell Nucleofector® Kit 1/ Lonza #VPH-5012) where 5 μg of plasmids were added previously ...
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bioRxiv - Immunology 2020Quote: ... Human buffy coats were initially added to 40 ml chemical-defined serum-free culture X-VIVO 15™ mediums (Lonza, Walkersville, MD, USA) and mixed thoroughly with 10ml pipette ...
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bioRxiv - Immunology 2022Quote: ... Activated cells were nuclofected with 100 µM AhR or non-targeting (NT1) siRNA (ON-TARGETplus SMART pool, Dharmacon) using the Amaxa Human T cell Nucleofector Kit (Lonza, Walkersville, MD, USA), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: The commercially available human myoblast (hMB) stock isolated from a healthy 35-year-old female (CC-2580, lot 0000483427; Lonza, Walkersville, MD, USA) was used [7,10] ...
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bioRxiv - Microbiology 2023Quote: ... plates were incubated at 37°C for 30 minutes in blocking buffer (PBS with 1% BSA, 5% FBS, and 1% human AB serum (Lonza, Walkersville, MD, USA), washed three times in PBS with 0.05% tween 20 and incubated with a commercial anti-SARS-CoV-2 Spike antibody (1/1000 ...
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bioRxiv - Cell Biology 2020Quote: ... HPAEC cells were procured from Lonza (catalog # CC-2530) and cultured in Endothelial Basal Media-2 (Lonza catalog #: CC-3516) supplemented with endothelial growth factors optimized for aortic and pulmonary arterial endothelial cells (Lonza catalog # CC-3162) ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of RNA were fractionated on a 2% NuSieve 3:1 agarose (Lonza), 1X MOPS ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293T cells (2 × 105) were resuspended with SF buffer (V4XC-2032, Lonza, Basel, Switzerland) and pulsed with purified SpyCas9 (20 pmol).
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bioRxiv - Physiology 2019Quote: HPMVECs were cultured in Microvascular Endothelial Cell Growth Medium-2 (EGM™-2MV, Lonza). TGF-β2 (2.5 ng/mL) ...
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bioRxiv - Immunology 2020Quote: ... and transduced with lentivirus to express CAR (MOI=2) in X-VIVO 15 (Lonza) containing 10% FCS with 5 μg/mL protamine sulfate (APP Pharmaceuticals) ...
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bioRxiv - Genomics 2019Quote: Bone marrow aspirates (donor n = 2, female, ages 22 & 24) were purchased from LONZA and hMSCs were isolated by adherence to tissue culture flasks for 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... were grown in EGM-2MV (Microvascular Endothelial Cell Growth Medium-2) medium from Lonza Bioscience ...
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bioRxiv - Immunology 2021Quote: ... coated dishes (5 ng/cm2) and cultured in Endothelial Growth Medium 2 (EGM2, Lonza) supplemented with 50 ng/mL VEGF with medium changes every other day until they reached confluency ...
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bioRxiv - Bioengineering 2021Quote: ... 2 mM L-glutamine and 50 units/ml penicillin/streptomycin (cDMEM; Lonza, Slough, UK). Soluble foam proteins were prepared as above ...
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bioRxiv - Bioengineering 2021Quote: ... were maintained in 0.1% (w/v) gelatin-coated flasks with complete EGM-2 (Lonza) supplemented with 1% penicillin-streptomycin (Gibco) ...
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bioRxiv - Bioengineering 2020Quote: ... Fluidic lines were coated with 1% gelatin which was replaced by EGM-2 (Lonza) for up to 24 hours ...
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bioRxiv - Bioengineering 2021Quote: ... Then cells were cultured in differentiation medium I consisting EBM-2 (Lonza, #CC-3156), 0.1% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... Talin1&2 double null cells (Atherton et al. 2015) were cultured in DMEM:F12 (Lonza) supplemented with 10% FCS (Lonza) ...
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bioRxiv - Cancer Biology 2022Quote: ... the culture medium was replaced with 2 mL of DMEM-F12 (LONZA #12-719F) supplemented with 10 % FBS ...
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bioRxiv - Neuroscience 2020Quote: ... Control HUVEC and HUVEC Nucleolin KD were cultured in the EGM-2 medium (Lonza), at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... Hep-2 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Lonza or Gibco) supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2022Quote: ... were cultured in EBM-2 endothelial cell growth basal medium (Lonza, Bend, OR, USA) supplemented with EGM-2MV microvascular endothelial cell growth medium SingleQuots supplements (Lonza) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR reactions were run on a 2% agarose gel (NuSieve™ GTG™, Lonza) and the 200-400 bp region was excised and purified using a QIAquick Gel Extraction Kit (#28704 ...
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bioRxiv - Cell Biology 2019Quote: ... Talin1/2 double null cells (Atherton et al., 2015) were cultured in DMEM:F12 (Lonza) supplemented with 10% FCS (Lonza) ...
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bioRxiv - Physiology 2019Quote: ... and cultured in EBM-2 medium supplemented with endothelial growth factors (EGM-2MV) (Lonza) for 14 days ...
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bioRxiv - Immunology 2021Quote: ... hECs were grown in endothelial cell growth medium (EGM-2 Bulletkit; Lonza, Basel, Switzerland) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting hiMPs were expanded in myoblast growth and proliferation media (Lonza; SKBM-2). All myogenic cells were differentiated in DMEM containing 2% horse serum ...
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bioRxiv - Biophysics 2022Quote: ... SAOS-2 cells were transfected using the SF buffer (Lonza, Cologne, Ger, V4XC-2012) and pulse code ‘DS-150’ ...
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bioRxiv - Immunology 2022Quote: ... for 4 days with complete EGM-2 media (Lonza CC-4147, Lonza CC-3156). Upon reaching 80-90% confluence ...
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bioRxiv - Immunology 2022Quote: ... for 4 days with complete EGM-2 media (Lonza CC-4147, Lonza CC-3156). Upon reaching 80-90% confluence ...
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bioRxiv - Microbiology 2024Quote: ... 10 μg of RNA were fractionated on a 2% NuSieve 3:1 agarose (Lonza), 1X MOPS ...