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Citations for Lonza :
1501 - 1550 of 1700 citations for HCC 1 CCL14 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... mouse skeletal myoblasts were grown at 37°C with a 5% CO2 humidified atmosphere in Dulbecco’s Modified Eagle’s Medium with 4.5 g.L−1 Glucose (DMEM; Lonza Bioscience, Bâle, Zwitzerland), supplemented with 10% fetal Bovin Serum (FBS ...
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bioRxiv - Molecular Biology 2019Quote: RPE-1 cells were transfected with 20 pmol siRNA by nucleofection (Amaxa Lonza 4D program EA-104 P3 solution) or Lipofectamine RNAiMAX transfection reagent (ThermoFisher ...
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bioRxiv - Genomics 2020Quote: ... 1 million U2OS cells were transfected using the Nucleofector 2b with 2 μg Plasmid DNA using kit V (Lonza) according the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... 2 × 105 organoid single cells were re-suspended with Lonza P3 nucleofection buffer and 1 µl of pmaxGFP (Lonza) and transferred to 20 µl nucleofection cuvette (V4XP-3024 ...
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bioRxiv - Neuroscience 2021Quote: ... Tissues were washed 3-4 times over 1 hour in PBS (calcium- and magnesium-free; Lonza BioWhittaker #17-517Q) containing 0.1% Triton X-100 (PBT ...
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bioRxiv - Cell Biology 2021Quote: ... dissected samples were pulled onto a coverslip containing an agarose pad made of 1% low-melt agarose (Lonza #50100) dissolved in Tyrode’s buffer ...
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bioRxiv - Cancer Biology 2019Quote: HEK293T was maintained in DMEM (Dulbecco’s modified Eagle’s high glucose medium) supplemented with 10% (v/v) FBS (foetal bovine serum) and 1% antibiotic/antimycotic (Lonza) in a humidified incubator with 10% CO2.
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bioRxiv - Systems Biology 2021Quote: ... Cells were transfected with 250 nM of siRNA and 1 μl of control pMax GFP (Nucleofector X kit, Lonza), using the CU-133 program ...
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bioRxiv - Immunology 2020Quote: ... Colons were incubated 2 times at 200 rpm in 40 mL HBSS + 0.1% BSA + 1% Penicillin-Streptomycin (PS, Lonza) + 5mM EDTA (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: HEK293T cells were maintained in DMEM (Dulbecco’s modified Eagle’s high glucose medium) supplemented with 10% (v/v) FBS (foetal bovine serum) and 1% antibiotic/antimycotic (Lonza) in a humidified incubator with 10% CO2.
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bioRxiv - Microbiology 2021Quote: ... were electroporated with 1 μg of Ssp1-linearized pcDNA4/TO Strep- tagged ADAP1 or GFP vector using Nucleofector2b (Lonza). Positive clones were selected with 10 μg/mL Blasticidin and 100 μg/mL Zeocin ...
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bioRxiv - Developmental Biology 2019Quote: ... were nucleofected into 1×106 pluripotent RUES2 cells using the B-016 setting on an Amaxa Nucleofector II (Lonza). Nucleofected cells were then plated as per maintenance conditions ...
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bioRxiv - Bioengineering 2019Quote: ... Transfection of transposon and transposase vectors was performed on day 2 with 0,5 µg transposon MC and 2 µg SB100X transposase RNA per 1×106 T cells on a 4D-Nucleofector according to the manufacturer’s instructions (Lonza). Transfected CD8+ and CD4+ T cells were expanded using the G-Rex culture system (Wilson-Wolf) ...
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bioRxiv - Bioengineering 2019Quote: K562 cells were transfected with two gRNAs targeting exon 1 and 3 of NGLY1 and Cas9 plasmid (lentiCas9-Blast) by Nucleofection according to the manufacturer’s protocol (Nucleofector, Lonza). LentiGuide-Puro (Addgene plasmid #52963 ...
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bioRxiv - Cancer Biology 2020Quote: 1×105 cells were suspended in 1 ml of 0.33% select agar in small airway growth medium without EGF (Lonza) and plated on a bottom layer of 0.5% select agar in the same media in six-well dishes ...
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bioRxiv - Cell Biology 2019Quote: ... Cells (1 × 106/10 cm dish) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; catalog no. 12-604F, Lonza) with 20% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2019Quote: ... Colons were incubated 2 times at 200 rpm in 40 mL HBSS + 0.1% BSA + 1% Penicillin-Streptomycin (PS, Lonza) + 5mM EDTA (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... the reaction was stopped by adding deactivated fetal calf serum (FCS; HyClone) and basic medium (1 % Penicillin/ Streptomycin (Lonza), 10 % FCS ...
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bioRxiv - Developmental Biology 2021Quote: ... All larvae were anaesthetised using 0.02% tricaine and then embedded on their side in 1% low melting agarose (Lonza) on a glass bottom dish (MalTEK Corporation ...
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bioRxiv - Developmental Biology 2020Quote: ... Wells were mounted by pouring 1 ml of 2.5% melted agarose (50080, Lonza Rockland, ME in Danieau buffer (DBx1) into the μ-dish with the mold inside ...
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bioRxiv - Cell Biology 2021Quote: ... The harvested EV pellet was resuspended in 1 mL RNase-free Phosphate buffered saline (RNase-free PBS, Lonza, Australia) and washed using ultracentrifugation at 100,000 × g for 60 min at 4°C ...
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Receptor tyrosine kinases regulate signal transduction through a liquid–liquid phase separated statebioRxiv - Cell Biology 2019Quote: ... A431 and MCF7 cells were maintained in DMEM (Dulbecco’s modified Eagle’s high glucose medium) supplemented with 10% (v/v) FBS (foetal bovine serum) and 1% antibiotic/antimycotic (Lonza) in a humidified incubator with 10% CO2 ...
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UC2 – A Versatile and Customizable low-cost 3D-printed Optical Open-Standard for microscopic imagingbioRxiv - Bioengineering 2020Quote: ... PBMCs were seeded at a density of 1 x 106cells/cm2 in X-VIVO 15 medium (Lonza, Cologne, Germany) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with PBS and 1 ml of fresh Hepatocyte Culture Medium (HCM) was added per well (Lonza). At 6 or 72 hpi ...
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bioRxiv - Cell Biology 2022Quote: ... OCI-LY-1 cells were transfected using the Amaxa® Cell Line Nucleofector® Kit L (Lonza, Basel, Switzerland), program C-05 as previously described(46) ...
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bioRxiv - Immunology 2022Quote: ... 1 x 106 T-cells were pelleted and re-suspended in 100 μL nucleofection solution (Lonza, Walkersville, MD, USA). T-cell suspensions were mixed with 5 μl RNP complex and cell/RNP mix transferred to Nucleofector cuvette ...
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bioRxiv - Molecular Biology 2024Quote: ... 33 μL of cell suspension was mixed with 66 μL of solution 1 (0.83% low-melting-point agarose SeaPlaque GTG (Lonza), 170 mM sorbitol ...
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bioRxiv - Molecular Biology 2024Quote: ... 33 μL cell suspension was mixed with 66 μL of solution 1 (0.83% low-melting-point agarose SeaPlaque GTG (Lonza), 170 mM sorbitol ...
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bioRxiv - Developmental Biology 2024Quote: ... anesthetized by incubation in egg water containing 0.003 % Tricaine and embedded laterally in 1 % low melting agarose (Lonza 50081) containing 0.16 mg/mL Tricaine in glass-bottom dishes (MatTek ...
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bioRxiv - Biophysics 2023Quote: ... tsa-201 cells were transfected via Lipofectamine 2000 24-48 hours prior to experiments with 1 μg pCMV6-SCN9A (gift from Dr. Christoph Lossin) and 0.5 μg pMaxGFP (Lonza) for identification of transfected cells ...
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bioRxiv - Bioengineering 2023Quote: ... single guide hybrids were mixed with 3uM Cas9 nuclease (Berkeley Labs) at a 1.2:1 ratio and delivered to cells by Lonza 3D (CA-137 ...
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bioRxiv - Immunology 2023Quote: moDCs were cultured with allogenic naïve CD4+ T cells in a 1:10 ratio in complete IMDM (IMDM (BE12- 722F, Lonza) with 100 U/mL penicillin ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×10E6 ME-1 cells were nucleofected with CRISPR/Cas9 plasmids (2 μg each) using Nucleofector Technology (Lonza Biologics) with the program X-01 and Amaxa Cell Line Nucleofector Kit V ...
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bioRxiv - Developmental Biology 2023Quote: ... Smiths Medical) were connected to two 1-mL gas-tight syringes (SGE or Hamilton) and filled with PBS (Lonza). The tubing was manually filled with PBS ...
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bioRxiv - Genetics 2023Quote: ... Fixed larvae were transferred to 0.17 mm imaging plates (FlouroDish) and embedded in 1% low melting agarose gel (SeaPlaque Agarose, Lonza) and imaged using a super resolution microscope-Leica STED 3X at 63x water lens.
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bioRxiv - Developmental Biology 2023Quote: ... MVECs were expanded and grown on 1% gelatin-coated dishes in endothelial basal medium-2 (EBM-2 (Lonza; 3156) with 10 % heat-inactivated FBS (Gibco) ...
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bioRxiv - Developmental Biology 2023Quote: ... using Vectashield® mounting medium (Vectorlabs, Newark, CA, USA) and embedded in 1% low melting agarose (Lonza, Basel, Switzerland). Images were obtained using a SP8 confocal microscope (Leica Microsystems ...
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bioRxiv - Cell Biology 2023Quote: ... 1xPenStrep (cat#XC-A4122, Biosera, Nuaille, France) and 1% nonessential amino acid (NEAA, cat#BE13-114E, Lonza, Basel, Switzerland) and maintained at 37 °C under 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... The sgRNA complexes were then added to 1×106 NK92 cells resuspended in 16uL of P3 nucleofection buffer (Lonza). The nucleofection mixture was transferred to a 16-well strip for nucleofection in the Lonza 4D Nucleofector using the pulse code CM-138 ...
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bioRxiv - Bioengineering 2023Quote: ... GV samples at final OD500 = 1.8 were loaded into the phantoms made of 1% agarose (w/v, Lonza, #50070) in those buffers and incubated at 37°C for >10 minutes before ultrasound imaging.
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bioRxiv - Cell Biology 2023Quote: ... Fluorescence microscopy to visualize lipid droplets was performed on mature adipocytes using AdipoRed (1:250 dilution, Lonza #PT-7009) and Hoechst 33342 (1 μg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... with two phosphothiorate linkages on each end were mixed with 82 µL Nucleofector Solution V and 18 µL of Supplement Solution 1 according to manufacturer’s recommendations for the Cell Line Nucleofector Kit V (Lonza). 5×105 U2OS.EGFP parent cells were resuspended in the above mixture and nucleofected with a Lonza Nucleofector 2b Device (Kit V ...
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bioRxiv - Biophysics 2024Quote: ... Nicked catenanes (treated with Nt.BsmAI - singly nicking both circles) were extracted using 1% low-melting point agarose (SeaPlaque GTG, Lonza) gels using β-Agarase I (NEB ...
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bioRxiv - Microbiology 2020Quote: ... the RNP complexes were added to 1×105 cells in 40 μl of P3 nucleofection buffer from the 4D-Nucleofector X kit (Lonza). Half of the mixture was loaded to each well of a 16-well nucleofection cassette and nucleofected using the using E0-100 program with the 4D-Nucleofector Lonza Amaxa ...
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bioRxiv - Cell Biology 2022Quote: ... peeled renal tubular epithelial cells were cultured in Primary medium (DMEM/F12, 10% FBS, 100U/mL penicillin, 100mg/mL streptomycin, amphotericinB, 1×REGM SingleQuots (CC-4127, Lonza)) in 6-well plates ...
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bioRxiv - Immunology 2021Quote: Control or transfected Lymphocytes (with Flag-wt-TDP-43 or Flag-NLS-mut-TDP-43 plasmid (1 μg) using nucleofection kit (Lonza)) were placed on coverslips (2 x 106 cells in sterile glass coverslips-Ø 12 mm ...
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bioRxiv - Genomics 2020Quote: 5 × 105 THP-1 cells were resuspended in 20µL nucleofection solution (16.4µL SG nucleofector solution + 3.6µL supplement 1) from the SG Cell Line 4D-Nucleofector™ X kit (Lonza, V4XC-3032). 300pmol PPP2R1A sgRNA (Synthego ...
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bioRxiv - Biochemistry 2019Quote: ... Sf21 cells (2 × 106 cells mL−1) were infected with the P3 viral stock in Insect-XPRESS protein-free medium (Lonza). The infected insect cells were grown at 27 °C with shaking for 66 hours ...
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bioRxiv - Neuroscience 2019Quote: ... Ganglionic eminences and cortex were dissected and dissociated cortical neurons were nucleofected with ON-TARGET plus mouse Mecp2 siRNA or Non-targeting siRNA 1 (Dharmacon) according to the protocol of Amaxa Nucleofection (Lonza). Then neurons were plated into microchambers coated with poly-D-lysine (0.1 mg/ml ...
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bioRxiv - Cancer Biology 2019Quote: 1-4 × 106 dissociated cells were re-suspended in 100 µL of Amaxa Mouse NSC Nucleofector Solution (VPG-1004, Lonza) with 5 µg of plasmid DNA ...