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Citations for Lonza :
1501 - 1550 of 1674 citations for 3 2 5 Dioxoimidazolidin 4 yl propanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... D-001206-14-05 5 nmol) and nucleofected into OPCs using the Basic Nucleofector Kit for Primary Mammalian Glial Cells (Lonza, VPI-1006) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels were fabricated using a solution consisting of lyophilized GelMA (5 wt%) dissolved at 37°C in phosphate buffered saline (PBS; Lonza 17-516F) and combined with 0.1% w/v lithium acylphosphinate (LAP ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lines were maintained at 37°C in a humidified incubator containing 95% air and 5% CO2 and routinely tested for mycoplasma contamination with the MycoAlert Assay (Lonza, LT07-418). For experiments requiring manipulation of cystine availability ...
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bioRxiv - Molecular Biology 2023Quote: Induced pluripotent stem cells were transfected with a total of 5 μg target Cas9/gRNA plasmids with or without 1 μg repair templates using Human Stem Cell Nucleofector Kit (LONZA, Basel, Switzerland) and Nucleofector 2b device (LONZA ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were maintained at 37°C and 5% CO2 in 2D monolayer culture and confirmed as Mycoplasma negative (MycoAlert Mycoplasma Detection Kit, Lonza #LT07-701). Cells were maintained in DMEM medium (Corning #10-013-CV ...
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bioRxiv - Microbiology 2024Quote: ... All cells were maintained in a humidified incubator with 5% CO2 at 37°C and tested negative for mycoplasma by MycoAlert (Lonza, Morristown, NJ).
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bioRxiv - Genetics 2024Quote: ... All the cells were grown with 5% CO2 at 37°C and verified mycoplasma-free using the MycoAlert Mycoplasma Detection Kit (Lonza, LT07-218). The differentiation of WTC11 i3N iPSCs into excitatory neurons was performed using a two-step differentiation protocol ...
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bioRxiv - Biophysics 2024Quote: ... Cells were mixed with buffer by pipetting the mixture up and down 5 times and electroporated using Lonza electroporator (Lonza 4D-Nucleofactor) using the CM130 pulse program ...
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bioRxiv - Synthetic Biology 2021Quote: ... The product was purified and 2 µg were added to the RNP and diluted in 100 µL P3 nucleofection buffer (Lonza). This mixture was nucleofected with 2×106 stimulated human primary T cells using the 4D-Nucleofector (Lonza ...
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bioRxiv - Cell Biology 2020Quote: U-2 OS cells stably expressing spdCas9-GFP were nuclofected with Amaxa® Cell Line Nucleofector® Kit V (Lonza). Cells were detached with 0.05% trypsin/EDTA and spun down by centrifugation at 1000rpm for 5 min ...
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bioRxiv - Immunology 2022Quote: ... a total of 1.6 x 108 cells were transfected with 2 μM of the corresponding ASO using the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza) and the EO-115 program ...
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bioRxiv - Developmental Biology 2020Quote: ... The red blood cell lysis was performed by resuspending the pellet in 2 ml sterile ACK (Ammonium-Chloride-Potassium) lysis buffer (ACK Lysing Buffer, Lonza) and incubating on ice for 5 minutes ...
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bioRxiv - Genomics 2021Quote: ... two million H9 hESCs were co-electroporated with the appropriate knockin vector (5 μg) and plasmids encoding AAVS1-targeting TALENs (2 μg; addgene, 59025 and 59026) using an Amaxa 4D Nucleofector system (Lonza). Serial cell dilutions were then seeded in six-well plates in E8 supplemented with Y-27632 (10 μM) ...
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bioRxiv - Bioengineering 2021Quote: ... The hCMEC/D3 cells were cultured in complete media composed of endothelial cell basal medium-2 (Lonza Walkersville Inc., MD), supplemented with 5% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... were resuspended at a density of 6×106 cells mL−1 in Microvascular Endothelial Cell Growth Medium-2 media (EGM-2MV, Lonza). The bottom channel of the chip was washed with EGM-2MV and loaded with 6 μL of HIMEC cell suspension (~36,000 cells per chip) ...
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bioRxiv - Cell Biology 2020Quote: ... aliquots of obtained BM aspirates were seeded into cell culture flasks containing endothelial basal media (EBM-2, Lonza, Cologne, Germany) supplemented with 10% human platelet lysate (PL ...
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bioRxiv - Bioengineering 2022Quote: ... Human hepatic stellate cell LX-2 was cultured in HEPES-buffered Dulbecco’s modified Eagle medium (DMEM) (Lonza BioWhittaker, Verviers, Belgium) supplemented with 4 mmol/L L-glutamine (Lonza) ...
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bioRxiv - Developmental Biology 2021Quote: ... and E15.5 (n=2–6) Six2-TROMA-1 stained kidneys were embedded in 1 % low melting agar (50100; Lonza Group). The lateral cortex of the kidneys was imaged with a Nikon A1R MP+ multiphoton microscope (Tokyo ...
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bioRxiv - Microbiology 2020Quote: ... for Vero-118 cells and serum reduced (2%) DMEM supplemented with 100 IU/ml penicillin-100 μg/ml streptomycin mixture (Lonza), 2 mM L-glutamine (Lonza) ...
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bioRxiv - Microbiology 2020Quote: ... and 3-fold (SARS-CoV-2 and SARS-CoV) serial dilutions of sample in serum-free EMEM supplemented with 20 µg/ml trypsin (Lonza) for MDCK cells ...
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bioRxiv - Bioengineering 2021Quote: ... SK-N-BE(2) or H460 cells were seeded at densities mentioned above in ultra-low adherent round-bottom 96-well plates (Lonza) for 72 hours ...
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bioRxiv - Immunology 2021Quote: ... Cells were plated at 2 x 106 cells and cultured in RPMI with 10% FCS with low endotoxin levels (Lonza). After 14 d ...
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bioRxiv - Cancer Biology 2022Quote: ... LSECs were counted and 105 cells were seeded in a 48-well plate and cultured in collagenated plates with EGM-2 medium (Lonza) for 3 days ...
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bioRxiv - Molecular Biology 2020Quote: ... pelleted at 350 xg for 10 minutes and resuspended in EBM-2 Basal Medium (CC-3156, Lonzs, Basel, Switzerland) supplemented with Microvascular Endothelial Cell Growth Medium-2 SingleQuots Supplements (CC-4147, Lonza) and 15% fetal bovine serum ...
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bioRxiv - Bioengineering 2020Quote: ... The CD31 negative fraction was then expanded (maximum of ten passages) on uncoated tissue culture plastic substrates supplemented with EGM-2 media (Lonza) and characterized (Fig ...
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bioRxiv - Cancer Biology 2021Quote: ... mixed with 2 µM control or 2 µM target-specific ASO and transferred to nucleocuvettes for nucleofection on a 4D-Nucleofector System (Lonza) using program code “DC-100” ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Successful test compounds from the mf assay were diluted to 10 μM and added to the trans-wells in 6 ml endothelial basal media with supplements (EGM-2 MV; Lonza). Twelve replicates (n = 6 wells ...
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bioRxiv - Microbiology 2021Quote: ... Cells were infected for 30 min with exponential-phase bacteria at a multiplicity of infection (MOI) of 2 and treated with 20 μg/ml of gentamicin (Lonza) for 60 min as described [22] ...
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bioRxiv - Microbiology 2021Quote: Purified SARS-CoV-2 RBD and SED (Spike ectodomain) were diluted in DPBS (without Ca and Mg, Lonza, 17-512F) and coated onto 96-well plates (MaxiSorp - ThermoFisher or High binding - Greiner Bio-one ...
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bioRxiv - Immunology 2021Quote: ... The inoculum was then removed and replaced with 2 ml of DMEM complete medium with 1% SeaPlaque agarose (Cat# 50100, Lonza). Plaques were visualized using Neutral red (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... HeLa (ATCC® CCL-2™), and HaCaT cells (gift from Jonathan Garlick, Tufts University) were grown in Dulbecco’s modified Eagle medium (DMEM; Lonza) containing high glucose ...
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bioRxiv - Cancer Biology 2020Quote: ... murine 3B11 and human HUVECs endothelial cells (all purchased from ATCC) were cultured in RPMI 1640+ 10% Fetal Bovine Serum (FBS) or in endothelial grown media (EGM-2-Lonza).
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bioRxiv - Immunology 2021Quote: ... The inoculum was then removed and replaced with 2□ml of DMEM complete medium with 1% SeaPlaque agarose (Cat. # 50100, Lonza). The cells were incubated at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... and soluble anti-CD28 (500 ng/mL) at a density of 2 × 106 cells/mL of X-vivo 15 serum-free medium (Lonza). For iTreg differentiation ...
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bioRxiv - Immunology 2020Quote: ... or media were cultured in 96 well flat bottom plates overnight then PBMC (2×105) from healthy donors were cultured at 37 °C in X-VIVO 15 media (Lonza) with 50-200 U/mL IL-2 (Peprotech) ...
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Incidence of an intracellular multiplication niche amongst Acinetobacter baumannii clinical isolatesbioRxiv - Microbiology 2021Quote: ... pH 7.4 and grown in supplemented keratinocyte growth medium containing 0.15 mM CaCl2 (KBM-2 BulletKit, Lonza Biosciences, Basel, Switzerland) as previously described (38) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were co-transfected with 2 µg of the CRISPR/Cas9 plasmid and 2 µg of ssODN (50 µM) using a Nucleofector II and Amaxa Nucleofector kits (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... at bead-to-cell ratio of 1:1 in human IL-2 medium (X-VIVO 15, serum-free hematopoietic cell medium, with 2mM L-Glutamine and gentamicin (Lonza) supplemented with 5% Human AB serum (Valley Medical) ...
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bioRxiv - Immunology 2023Quote: ... Transfections were performed with an Amaxa Nucleofector device: 2×106 cells were resuspended in 100 μL Nucleofector solution T (Lonza) and added to 2 µg of pcDNA3.1 expression vector encoding full-length IL-2Rβ ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 µg of PX459 V2.0 containing the sgRNA sequence were electroporated into 2×106 DVI2 ESCs (HM1 background) using program A023 of the Amaxa Nucleofector II (Lonza) to ablate endogenous Shh gene expression ...
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bioRxiv - Physiology 2022Quote: HUVEC were grown at 80% confluence (5×103 cells per well) on glass coverslips in a 24-well plate and starved overnight in serum-free medium (EBM-2, Lonza). They were then incubated for 24 hr in EGM2 containing 10μM BrdU ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were washed twice with PBS and 2 × 106 cells were resuspended in 100 μl of Solution V (Lonza) and then mixed with 5 μg purified sgRNA expression vector or the empty vector as a negative control before nucleofection using program Q-001 of a Nucleofector™ 2b (Lonza) ...
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bioRxiv - Bioengineering 2024Quote: ... HSPCs (2×105 cells/condition) were transfected with RNP complexes using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza) and the CA137 program (Nucleofector 4D ...
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bioRxiv - Bioengineering 2024Quote: ... NHDF cells were cultured in FBM fibroblast basal media (supplemented with FGM-2 SingleQuot supplements: insulin, hEGF-B, GA-1000, and FBS) (Lonza). NHEK cells were expanded in KBM gold basal medium (KGM gold supplemented with Lonza’s SingleQuot supplements ...
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bioRxiv - Cancer Biology 2024Quote: ... were isolated from 8 to 16-week-old Rptorfl/fl or Tsc2fl/fl mice and maintained in EGM-2 medium (Lonza), as previously described71–75 ...
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bioRxiv - Cancer Biology 2024Quote: ... The OCI-LY3 cell line was cultured in IMDM with 25 mM HEPES and 2 mM L-glutamine (Lonza; Switzerland), supplemented with P/S ...
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bioRxiv - Cell Biology 2024Quote: ... Large scale D.Mel-2 cultures were prepared by diluting cells to a density of 106 cells/mL in Insect Xpress culture medium (Lonza) in Erlenmeyer flasks ...
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bioRxiv - Cancer Biology 2024Quote: ... by flushing one femur with PBS 2% FBS followed by erythrocyte elimination with ACK Lysing Buffer (10-548E, BioWhittaker, Lonza) (Figure 4BC) ...
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bioRxiv - Cell Biology 2023Quote: ... JAWS transfection was achieved using 20 μg DNA and 2 x 106 million cells per 100 μl nucleofection solution from the Amaxa Nucleofector Mouse Dendritic Cell kit (Lonza), and the Y-001 program ...
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bioRxiv - Developmental Biology 2023Quote: ... An mTmG lens carrying the MLR39-Cre allele was placed in a small hole in the center of an agarose pad that was prepared by loading 1 mL of 2% agarose (SeaKem GTG, Lonza) in M199 on the surface of 35 mm glass-bottom dish (3970-035 ...