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Citations for Lonza :
1501 - 1550 of 2319 citations for 2R 5S 5 4 amino 5 fluoro 2 oxopyrimidin 1 2H yl 1 3 oxathiolan 2 yl methyl butyrate WXC04778 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... The purity of the final recombinant proteins was determined to be more than 99% by sodium dodecyl sulfate and polyacrylamide gel (SDS-PAGE) with an endotoxin concentration lower than 2 units/mg protein measured by Limulus Amebocyte Lysate PYROGENT™ 125 Plus (Lonza). In previous experiments we demonstrated that these recombinant proteins were functionally devoid of significant endotoxin contamination ...
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bioRxiv - Biochemistry 2021Quote: ... 500,000 cells were seeded per well and 2 days later the experiment was started by replacing the culture medium with 2.5 ml Pro293a medium (Lonza, Basel, Switzerland), supplemented with 2 mM L-glutamine and 100 units pen/strep (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... and Subset Four (podoplanin+CD36+) were sorted into 5mL FACS tubes containing endothelial basal media (EGM-2 medium without supplements added, Lonza, USA) supplemented with 10% FBS at 4°C degrees ...
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bioRxiv - Physiology 2022Quote: ... A second enrichment with anti-CD102-coated Dynabeads was performed before culturing cells at 37Cº on fibronectin-coated tissue culture dishes in EGM-2 media (Lonza Walkersville). Cells (passage two ...
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bioRxiv - Neuroscience 2024Quote: ... digested for 3 min at 37 °C in Accutase and then resuspended in 60 μL nucleofection buffer from the Human Stem Cell Nucleofector™ Kit 2 (Lonza). The suspension was combined 2 μM Electroporation Enhancer (IDTDNA ...
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bioRxiv - Microbiology 2024Quote: ... falciparum strains were cultured at 2% haematocrit in O+ erythrocytes and RPMI-1640 medium (Lonza, BE12-167F or Gibco, 31870-025) supplemented to give the following final concentrations ...
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bioRxiv - Molecular Biology 2024Quote: ... HSVECs were obtained by enzymatic collagenase digestion of human saphenous veins (Ethics 15/ES/0094) and maintained in EC growth medium (EGM-2 BulletKit™) (Lonza) supplemented with foetal bovine serum (10% ...
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bioRxiv - Immunology 2024Quote: ... and spun at 200xg for 10 min and resuspended at a concentration of 2-10x106 cells/100µL in Lonza Buffer P3/supplement (Lonza #V4XP-3024). The RNP complex and 100µL of resuspended cells were combined and electroporated using pulse code EO-115 on the Lonza 4D-Nucleofector Core/X Unit.
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bioRxiv - Microbiology 2024Quote: ... Passage 2 cells were transferred to 75 mL flasks and grown in confluence in Dulbecco Modified Eagle medium (DMEM) (Lonza BioWhittaker) containing 10% heat-inactivated fetal bovine serum (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... with L-ascorbic acid-2-phosphate (AA2P) (5mg/ml stock, 10 µl/ml), and β-glycerolphosphate (BGP) (20 mM stock, 20 µl/ml) (Lonza). The cell culture medium was changed every 3 days ...
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bioRxiv - Bioengineering 2024Quote: ... HepG2 and K562 cells (2×105 cells/condition) were electroporated using the SF Cell Line 96-well Nucleofector™ Kit (Lonza) and the CM-130 ...
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bioRxiv - Cell Biology 2023Quote: ... Human visceral pre-adipocytes were purchased from Lonza (PT-5005) and cultured according to the manufacturer’s instructions in PGM-2TM Preadipocyte Growth Medium-2 BulletKitTM from Lonza (PT-8002). Once cells were expanded ...
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bioRxiv - Cancer Biology 2023Quote: ... HUVEC cells were maintained in growth factor-supplemented media (EBM + EGM-2 aliquots + 10% FBS) from Lonza (C-3121, CC-4542). MDA-MB-231 cell line was modified to express GFP (green fluorescent protein ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of the final 100 μM siRNA duplex was mixed with 100 μL of Nucleofection solution V (Lonza, VVCA-1003) and transfected into 10 million cells using a NucleofectorTM 2b Device and program T-029 (Lonza) ...
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bioRxiv - Cancer Biology 2024Quote: ... K562 and NALM6 cells (2×105 cells/transfection) were transfected using a Lonza 4D nucleofector™ with a SF nucleofection kit (Lonza) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μL of the final 100 μM siRNA duplex was mixed with 100 μL of Nucleofection solution V (Lonza, VVCA-1003) and transfected into 4 million cells (48 h knockdown ...
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bioRxiv - Bioengineering 2024Quote: ... Liver sinusoidal endothelial cells were isolated using CD146 microbeads (Miltenyi Bio 130-092-007) according to manufacturer’s instructions and cultured in EBM-2 media with supplements (Lonza CC-3162). LSECs were plated at 2×104 cells per well in 96-well tissue culture plates ...
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bioRxiv - Cancer Biology 2023Quote: All cell lines were cultured at 37°C and 5% CO2 and tested for mycoplasma contamination using a MycoAlert® mycoplasma detection kit (Lonza, cat: LT07-218). Only mycoplasma-negative cells were used.
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bioRxiv - Genetics 2023Quote: ... of sg1617 or sg1618 was mixed with 500 pmol (5 μM) of 3xNLS-SpCas9-SpCas9 protein and added electroporation buffer (Lonza 4D, cat# V4XP-3024) up to 25 μl in one tube ...
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bioRxiv - Genetics 2023Quote: ... of sg1617 or sg1618 (see sequences of sgRNAs in Table S2) was mixed with 100 pmol (5 μM) of 3xNLS-SpCas9 protein and added electroporation buffer (Lonza 4D, cat# V4XP-3032) up to 5 μl in one tube ...
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bioRxiv - Bioengineering 2023Quote: Cells were cultured at 37°C and 5% CO2 in cell-specific media: primary human umbilical vascular endothelial cells (HUVECs, Lonza; up to passage 6) in EGM-2 (Lonza ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were maintained at 37°C with 5% CO2 and were regularly tested for Mycoplasma contamination using the MycoAlert PLUS kit (Lonza, Cat. No. LT07 −705).
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bioRxiv - Genomics 2024Quote: ... 300 pmol (15 μM) of sg1617 was mixed with 100 pmol (5 μM) of 3xNLS-SpCas9-HiFi protein and added electroporation buffer (Lonza 4D, cat# V4XP-3032) up to 5 μl in one tube ...
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bioRxiv - Cancer Biology 2020Quote: UWB1.289 and UWB1.289+BRCA1 cells were purchased from ATCC and were maintained in 1:1 MEBM (Lonza): RPMI 1640 with GlutaMAX (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: HNE1 cells were cultured in 1:1 mixture of Dulbecco modified Eagle medium (DMEM; Lonza; 12-604Q) and Ham’s F-12 ...
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bioRxiv - Immunology 2021Quote: ... Cells were suspended in 90μL of a 1:1 mixture of Matrigel:modified SAGM media (CC-3118, Lonza). SAGM media is modified by adding 0.1 μg/mL cholera toxin (C8052 ...
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bioRxiv - Cell Biology 2021Quote: ... consisting of (1:1 mixture of Renal Cell Growth Medium (REBM) medium supplemented with REGM SingleQuots (Lonza) and DMEM high glucose (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... UWB1 and UWB1+BRCA1 cells were maintained in RPMI1640 (ATCC) and MEGM bullet kit (1:1; Lonza) with 3% FBS ...
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bioRxiv - Bioengineering 2021Quote: ... HUVECs (Lonza, Passage <4) were cultured in an endothelial growth medium (C-22111 ...
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bioRxiv - Systems Biology 2020Quote: ... The pellets were resuspended in 10 mL of complete mammary epithelial growth medium with 2% v/v FBS without GA-1000 (MEGM; Lonza CC-3150). Cells were incubated at 37 °C for 1 h ...
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bioRxiv - Cell Biology 2021Quote: Genes were knocked out using 1-2 synthetic sgRNAs (Synthego) in complex with spCas9 (Aldeveron 9214) that were electroporated into cells using a nucleofector system (Lonza V4XC- 1032) according to published methods (Hoellerbauer et al. ...
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bioRxiv - Biophysics 2021Quote: All experiments using HUVEC were performed with an hTERT-immortalized HUVEC line previously described (28) and cultured in EGM-2 media (Lonza, CC-3162). For transient DNA transfections ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HUVEC cells were grown at 37° C in the Endothelial cell basal medium-2 (LONZA #Cat No-CC-3156 and CC-4176) under the atmosphere containing 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... 1.2E6 BHK21 cells were nucleofected with 2 µg of Spike plasmid using an Amaxa Nucleofector II with cell line kit L (Lonza #VCA-1005) and program A-031 ...
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bioRxiv - Systems Biology 2022Quote: ... Early passage HUVECs were cultured in EGM™2- Bulletkit™ medium with growth supplements CC-3156 & CC-4176 purchased from Lonza. At 80% confluency ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were maintained in RPMI 1640 medium supplemented with 2 mM Glutamine and 10% (v/v) heat-inactivated FBS (Lonza Group Ltd.) containing 100µg streptomycin ml−1 and 100 U penicillin ml−1 and passaged as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and CRX +/tdtomato were maintained in reduced growth factor Matrigel (50ug/cm^2) in mTeSR1 plus with 1x Pen/Strep Amphotericin B (Lonza, 17-745E).
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bioRxiv - Molecular Biology 2021Quote: ... Myoblasts derived from an adult DM1 patient biopsy (DM-05 (Xia et al., 2013)) were cultured in SkGM-2 skeletal muscle cell growth medium (Lonza, #CC-3245).
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bioRxiv - Molecular Biology 2021Quote: ... 10 μg of pX330 neomycin-resistant vector containing each gRNA and 10 mM ssODN were co-transfected into 2 × 106 ESCs using a Nucleofector (Lonza, program A023). Cells were plated onto two 10 cm dishes containing hygromycin-resistant DR4 MEFs ...
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bioRxiv - Immunology 2020Quote: ... 2 million L1.2 cells were transfected with 2 µg of plasmid using the SG Cell Line transfection kit and a 4D-Nucleofector X (both from Lonza, Walkersville, MD) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 1 × 106 BLaER1 cells were transfected with 2 μg plasmid DNA using the Human B Cell Nucleofector Kit and Nucleofector I device (program U-15; both Lonza, Basel, Schweiz). On day 2 post transfection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... UCB-MNC were thawed and cultured at 2 million cells/mL in X-VIVO 15 serum-free cell-culture medium (Lonza, Basel, Switzerland) supplemented with 0.5 μg/mL of FMS-like tyrosine kinase-3 and 0.5 μg/mL of stem-cell factor ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HUVEC cells were grown at 37o C in the Endothelial cell basal medium-2 (LONZA #Cat No-CC-3156 and CC-4176) under the atmosphere containing 5% CO2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... were ligated to the target fragments and full-length ligation products were purified via gel electrophoresis (2% MetaPhor agarose, Lonza, Basel, Switzerland). Purity and concentration of extracted fragments were determined using capillary electrophoresis (Fragment Analyser ...
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bioRxiv - Microbiology 2022Quote: ... pre-coated cell culture 6-well plate with EBM-2 endothelial media supplemented with 10% FBS and a growth factor bullet kit (Lonza, Walkersville, USA) 3 mL/well ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultivated together with mixed glial cells plated on the bottom of 12-well plates for 7 days in EBM-2 medium (Lonza, Basel, Switzerland) containing 15% PDS ...
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bioRxiv - Microbiology 2024Quote: ... Non-adherent cells were centrifuged again and resuspended in 2 mL of bronchial epithelial cell growth medium (BEGM) (Lonza cat# CC-3170) supplemented with Primocin (InvivoGen ...
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bioRxiv - Cancer Biology 2024Quote: Fresh single cell suspension remaining from PDOTS processing (S3) was grown in SmGM-2 Smooth Muscle Cell Growth Medium (Lonza, CC-3181) supplemented with SmGM-2 SingleQuots Supplement Pack (Lonza ...
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bioRxiv - Bioengineering 2023Quote: ... were cultured in Endothelial Cell Basal Medium (EBM) supplemented with the Endothelial Growth Media kit (EGM-2) (CC-3162, Lonza, Basel, Switzerland). All cells were maintained at 37°C and 5% CO2 and used between passage number 3-7 ...
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bioRxiv - Immunology 2023Quote: ... 80 µl of supernatant was removed and replenished with 100 µl IMDM/Glutamax/ 10% FBS containing 2× MycoZap Plus-PR (Lonza #VZA-2021), 100 ng/ml human IL-2 (GoldBio #1110-02-50) ...