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Citations for Lonza :
101 - 121 of 121 citations for Synapsin I derived peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... HeLa Myo10 KO1 cells were nucleofected using an Amaxa nucleofection apparatus (Lonza; program I-013) with Piggybac plasmid and either WT Xlone-mScarlet-Myo10 or mutant Xlone-mScarlet-Myo10 ...
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bioRxiv - Immunology 2023Quote: ... CTLs derived from LifeAct-GFP expressing pmel-1 mice were incubated with peptide-loaded and IFNγ-treated B16 cells cultured in phenol red-free DMEM (Lonza) supplemented with 10% FBS on stage at 37°C ...
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bioRxiv - Immunology 2024Quote: ... and HLA-A2+ T2 cells (can be efficiently loaded with exogenous peptides as they lack the transporter associated with antigen processing) were cultured in RPMI 1640 media (Lonza) supplemented with 10% FCS (Merck) ...
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bioRxiv - Biochemistry 2020Quote: ... were cultured on collagen I-coated dishes with EGM-2MV medium (CC-3202; Lonza Japan, Tokyo, Japan) at 37°C under 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... 5×104 APC were seeded together with 1×105 CD4+ T cells in the presence of relevant peptides at 2.5 ng/ul diluted in X-VIVO 15 medium (Lonza, Basel, Switzerland) supplemented with 4 % human AB plasma (Innovative Research ...
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bioRxiv - Cell Biology 2022Quote: ... cells were counted using Trypan Blue Exclusion Method and seeded into Collagen-I coated plates at a density of approximately 100,000 cells/cm2 following the Lonza’s instructions (Lonza, “Suspension and Plateable Cryopreserved Hepatocytes Technical Information and Instructions”) ...
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bioRxiv - Biophysics 2021Quote: ... 10 pmol of the cholesterol-modified reverse primer and 1μL 20X SYBR® Green I Nucleic Acid Stain (Lonza) were mixed with the KAPA2G Fast HotStart ReadyMix in a total volume of 20 μL ...
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bioRxiv - Immunology 2021Quote: ... washed in PBS by centrifugation for 5 min at 1300 rpm at 4°C and resuspended at 4.0 × 106 cells/ml in complete medium (IMDM 2 mM glutamax I supplemented with 8% heat-inactivated FCS (Lonza), 2% heat-inactivated chicken serum ...
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bioRxiv - Molecular Biology 2020Quote: ... at Day 3-4 of phase I culture were electroporated using P3 Primary Cell 4D NucleofectorTM X Kit (from Lonza) with program DZ100 (Bak et al. ...
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bioRxiv - Bioengineering 2020Quote: ... 8 volumes of collagen I gel solution (10mg/ml) were mixed with 1 volume of 10X EMEM (Lonza: 12-684F) and 1 volume of 10X Reconstruction buffer(Anacker and Moody 2012 ...
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bioRxiv - Molecular Biology 2020Quote: ... Freshly isolated HBECs were plated onto rat tail collagen type I-coated (10 μg/ml) dishes in Bronchial Epithelial Growth Medium (BEGM, Lonza) containing 1% antibiotic– antimycotic solution (AA ...
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bioRxiv - Microbiology 2023Quote: ... 190 μL was mixed with 10 μL of 20X SYBER Green I nucleic acid stain (Lonza Walkersville Inc, Walkersville, MD) in a well of a transparent 96-well plate and incubated in the dark for 15 minutes before being placed in the Guava easyCyte HT flow cytometer (488 nm ...
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bioRxiv - Pathology 2022Quote: ... using a Mitotracker® Deep RedFM (molecular probes, 50μg, reference M22426) and SYBR™ Green I (10,000x concentrate in DMSO, Lonza) staining ...
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bioRxiv - Bioengineering 2023Quote: ... were expanded in type I collagen precoated culture flasks in endothelial cell growth medium-2 (EGM-2) BulletKit medium (CC-3162, Lonza). Culture flasks were precoated with 50 μg ml−1 collagen I rat tail (354236 ...
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bioRxiv - Cell Biology 2023Quote: ... hydrogels were prepared by diluting collagen I stock to a final concentration of 2.5 mg/mL with 10% v/v 10x MEM-α (BE12-684F, Lonza), 10% v/v foetal bovine serum ...
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bioRxiv - Plant Biology 2023Quote: ... Quantitative real-time PCR reactions with Taq polymerase prepared following (Pluthero, 1993) and SYBR Green I Nucleic Acid Gel Stain (Lonza) was performed in triplicates using the CFX96 real-time PCR detection system (Bio-Rad ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: The siRNA knockdown was performed by electroporation using the Amaxa nucleofector I in combination with the Amaxa Cell Line Nucleofector Kit V (Lonza, VCA-1003). SiRNA constructs with the siRNA cloned in the pLKO.1-Puro vector were obtained from the MISSION shRNA library (Sigma product SHGLY) ...
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bioRxiv - Cancer Biology 2020Quote: ... Single cells from digested mammary glands were resuspended and plated on Collagen I-coated plates (50μg/ml) in stem cell-enriching medium MEBM (Lonza Cat. #CC-3151), supplemented with 2% B27 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... OC activity was measured by releasing the europium conjugated human Type I collagen-coated on the bottom of the OsteoLyse™ Assay Kit (Lonza Biosciences) at each time point ...
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bioRxiv - Immunology 2020Quote: ... 1 × 106 BLaER1 cells were transfected with 2 μg plasmid DNA using the Human B Cell Nucleofector Kit and Nucleofector I device (program U-15; both Lonza, Basel, Schweiz). On day 2 post transfection ...
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bioRxiv - Cancer Biology 2019Quote: All tumouroids were fabricated using monomeric Type I rat-tail collagen (First Link, Birmingham, UK) and the RAFT™ protocol pages 8-9 (Lonza, Slough, UK) as previously described21 ...