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Citations for Lonza :
101 - 150 of 1883 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... opaque 96-well culture plates and survival was measured using the ViaLight Assay (Lonza Group Ltd., Rockland, ME).
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bioRxiv - Molecular Biology 2024Quote: ... using ToxiLight bioassay kit (Lonza) according to the manufacture’s recommendations.
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bioRxiv - Microbiology 2023Quote: A ToxiLight BioAssay Kit (Lonza) was used to determine cell death ...
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bioRxiv - Microbiology 2023Quote: ... and Nucleofector Kit R (Lonza) following manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... A MycoAlert detection kit (Lonza) was subsequently utilized for Mycoplasma screening ...
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bioRxiv - Cancer Biology 2022Quote: ... To assay anoikis resistance and anchorage-independent proliferation IEC6 cells were grown in 0.35% low-melt agarose (Lonza SeaPlaque). A base layer of agarose was prepared by melting pre-sterilized 1.2% agarose in dH2O at 75°C ...
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bioRxiv - Microbiology 2020Quote: ... Stock titer was determined by plaque assay (described below) on HeLa cells overlaid with MEM (Lonza, Walkersville, MD, USA) + 0.5% agarose (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Infections for plaque assay in Vero E6 cells were performed in Eagle’s minimal essential medium with 25 mM HEPES (EMEM; Lonza) supplemented with 2% FCS ...
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bioRxiv - Immunology 2021Quote: ... and the levels of endotoxins were measured using the Chromogenic Limulus Amebocyte Lysate QCL-1000 Assay (Lonza, Walkersville, MD). Fh12 was considered endotoxin free when endotoxin levels gave similar to background levels (<0.1 EU/ml) ...
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bioRxiv - Cell Biology 2024Quote: ... All cell lines were tested for mycoplasma at each batch freezing by PCR (32) and biochemical assay (MycoAlert, Lonza).
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bioRxiv - Microbiology 2023Quote: ... and the absence of endotoxin was confirmed using the Chromogenic Limulus Amebocyte Lysate QCL-1000 Assay (Lonza, Walkersville, MD). nFhGST was concentrated by AMICON Ultra-15 Centrifugal filter unit and adjusted to 1 mg/ml as determined using the Pierce protein bicinchoninic acid (BCA ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lines were routinely verified via STR genotyping and tested for mycoplasma contamination using the Lonza MycoAlert assay (Lonza). Below is a list of cell line details:
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bioRxiv - Cancer Biology 2023Quote: Invasion assays were performed using human bronchial epithelial cells grown on collagen discs containing primary human pulmonary fibroblasts (Lonza Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... Presence of mycoplasma in culture was assessed using the MicoAlert assay control test according to the manufacturer’s instructions (cat #LT07-518, Lonza). Sanger sequencing was performed to verify the presence of pathogenic variant in patient cell lines (clones 11 and 13 ...
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bioRxiv - Immunology 2020Quote: ... The MycoAlert Mycoplasma Detection Kit (Lonza) was used to monitor mycoplasma contamination on a regular basis.
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bioRxiv - Immunology 2021Quote: ... and Nucleofector kits (Lonza, VPB-1002) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... MycoAlert Mycoplasma Detection Kit (Lonza, Bioscience). Each cell lines were used within passage 4/5 since their thawing from originally frozen vials.
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bioRxiv - Cancer Biology 2022Quote: ... Mycoplasma testing (MycoAlert Detection Kit, Lonza) was performed monthly.
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bioRxiv - Cell Biology 2022Quote: ... using MycoAlert detection kit (Lonza, LT07). Additionally ...
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bioRxiv - Cancer Biology 2022Quote: ... Regular testing with MycoAlert kit (Lonza) confirmed the absence of mycoplasma contamination ...
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bioRxiv - Cancer Biology 2020Quote: ... using MycoAlert™ kit (Lonza, Germany) and genetically authenticated using a STR-PCR fragments kit (Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... Nucleofector Kit V was from Lonza. The original vector pCasper-GR (#FP971 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with the SingleQuots kit (Lonza), 5 μg/ml transferrin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Nucleofector Kit V (Lonza, VCA-1003); paraformaldehyde (Electron Microscopy Sciences ...
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bioRxiv - Microbiology 2024Quote: Toxilight Bioassay Kit (Lonza, LT17-127) or LDH-Glo Cytotoxicity Assay (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... Mycoplasma detection kit (Lonza #LT07-318) was used as per the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... and verified mycoplasma free by Lonza MycoAlert mycoplasma detection kit (LT07, Lonza). Fibroblasts were synchronized by temperature cycles of 12 h 32°C followed by 12 h 37°C for 5 days ...
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bioRxiv - Cancer Biology 2023Quote: Cell□line□specific Nucleofector Kits (Lonza) were used for transfections which were performed according to manufacturer’s protocol on a Nucleofector® 2b Device (Lonza) ...
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bioRxiv - Cell Biology 2023Quote: ... using MycoAlert detection kit (Lonza, LT07). For co-immunoprecipitation experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... Nucleofector Kit V (Lonza, VVCA-1003), T-029 program on NucleofectorTM 2b Device (Lonza) ...
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bioRxiv - Cancer Biology 2024Quote: ... Amaxa Cell Line Nucleofector Kit (Lonza) was used for electroporating the cells with the plasmids ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lines were routinely validated by functional assay and western blot as appropriate and tested monthly for mycoplasma contamination by MycoAlert (Lonza). Transfection of plasmid DNA was performed with Lipofectamine 3000 and P3000 reagent (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... and human ACTB (assay control, primer composition not disclosed by manufacturer) were loaded on a 2.5% agarose gel (Lonza, 50010) and stained with GelRed® (Merck ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines used in this study were authenticated using short tandem repeat genotyping (Labcorp) and confirmed to be negative for mycoplasma contamination using MycoAlertTM PLUS Assay (Lonza).
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bioRxiv - Cell Biology 2020Quote: ... The purity of CyaA batches is higher than 90% as judged by SDS PAGE analysis and contained less than 1 EU of LPS/μg of protein as determined by a standard LAL assay (Lonza). Finally ...
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bioRxiv - Immunology 2020Quote: ... Endotoxin concentrations ranged from 150-175 EU/mL as determined using the limulus amebocyte lysate assay (Lonza, Walkersville, MD, USA). This concentration of ODE has been previously shown to produce optimal experimental effects and is well-tolerated in mice (11 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were confirmed to be mycoplasma-free based on a luminescence-based enzymatic assay (MycoAlert®, Lonza, Cat# LT07-118) conducted within 1 week of the commencement and completion of the experiments ...
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bioRxiv - Cell Biology 2023Quote: ... All sorted and parental cell lines were confirmed to be mycoplasma-negative in a MycoAlert PLUS assay (Lonza, LT07-710) performed by the Koch Institute High-Throughput Sciences Facility (Cambridge ...
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bioRxiv - Cell Biology 2023Quote: ... BM CD34+ cells from young healthy donors for functional assays were collected at San Raffaele Hospital or purchased from Lonza. For the phenotypic analyses the sample size was determined by the number of individuals for whom excess material was available ...
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bioRxiv - Developmental Biology 2024Quote: ... Sorted cells expressing medium levels of fluorescence were expanded and frozen then representative lots of each cell line were thawed and tested for mycoplasma contamination using the MycoAlert assay (Lonza), and fungi and aerobic bacteria sterility by inoculating conditioned medium into trypticase soya broth (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... and the manufacturer’s instructions were followed (PyroGene Recombinant Factor C Endpoint Fluorescent Assay-#50-658U, Lonza Bioscience, Walkersville, MD, USA). Serum samples were diluted at 1:50 for LBP analysis ...
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bioRxiv - Molecular Biology 2021Quote: Cells were regularly checked for mycoplasma contamination using a luminescence-based kit (Lonza, MycoAlertTM Mycoplasma Detection Kit).
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bioRxiv - Bioengineering 2024Quote: ... Electroporation kit V and Electroporation kits for Primary T-Cell/HSPC were purchased from Lonza (Basel, Switzerland) and used on the Lonza Nucleofector 2b system ...
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bioRxiv - Cell Biology 2020Quote: ... The adipogenic potential was assessed after 14 days of exposure to induction media by Oil Red O staining to observe lipid droplets and AdipoRed™ assay (Lonza).
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bioRxiv - Immunology 2023Quote: ... acetylated chitin oligomers were suspended in endotoxin free water and tested for endotoxin level by using the limulus amebocyte lysate (LAL) assay (Lonza, CH). Levels below 0.25 EU/ml (<25 pg/ml LPS ...
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bioRxiv - Synthetic Biology 2024Quote: ... using SF Cell line 4D-Nucleofector X Kit L or X Kit S (Lonza, V4XC-2024, V4XC-2032) with the program CQ-104 ...
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bioRxiv - Cancer Biology 2021Quote: ... Kit S (both from Lonza, Basel, Switzerland). HDR was stimulated by HDR enhancer (IDT ...
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bioRxiv - Bioengineering 2021Quote: ... and EGM-2 SingleQuot bullet kit (Lonza). For imaging experiments ...
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bioRxiv - Neuroscience 2020Quote: ... and the Mouse Neuron Nucleofector Kit (Lonza), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and the Human Monocyte Nucleofector Kit (Lonza) as previously described (Schnoor et al. ...