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Citations for Lonza :
1351 - 1400 of 2561 citations for Butyrylcholinesterase Fluorescent Activity Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Co-transfection of 500 ng sgRNA plasmid and 1 µl of 10 µM ssODN template into 2×105 HEK293 cells was performed using nucleofection on the 4D-Nucleofector system (LONZA, Basel, Switzerland), as described previously38 ...
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bioRxiv - Systems Biology 2020Quote: ... The pellets were resuspended in 10 mL of complete mammary epithelial growth medium with 2% v/v FBS without GA-1000 (MEGM; Lonza CC-3150). Cells were incubated at 37 °C for 1 h ...
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bioRxiv - Cell Biology 2021Quote: Genes were knocked out using 1-2 synthetic sgRNAs (Synthego) in complex with spCas9 (Aldeveron 9214) that were electroporated into cells using a nucleofector system (Lonza V4XC- 1032) according to published methods (Hoellerbauer et al. ...
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bioRxiv - Biophysics 2021Quote: All experiments using HUVEC were performed with an hTERT-immortalized HUVEC line previously described (28) and cultured in EGM-2 media (Lonza, CC-3162). For transient DNA transfections ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HUVEC cells were grown at 37° C in the Endothelial cell basal medium-2 (LONZA #Cat No-CC-3156 and CC-4176) under the atmosphere containing 5% CO2 ...
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bioRxiv - Systems Biology 2022Quote: ... Early passage HUVECs were cultured in EGM™2- Bulletkit™ medium with growth supplements CC-3156 & CC-4176 purchased from Lonza. At 80% confluency ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were maintained in RPMI 1640 medium supplemented with 2 mM Glutamine and 10% (v/v) heat-inactivated FBS (Lonza Group Ltd.) containing 100µg streptomycin ml−1 and 100 U penicillin ml−1 and passaged as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and Ode macrophages (17) were cultured in RPMI 1640 medium supplemented with 2 mM of L-glutamine (Lonza, catalogue number 12-702F) and 10 mM Hepes (Lonza ...
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bioRxiv - Cell Biology 2021Quote: ... and CRX +/tdtomato were maintained in reduced growth factor Matrigel (50ug/cm^2) in mTeSR1 plus with 1x Pen/Strep Amphotericin B (Lonza, 17-745E).
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bioRxiv - Molecular Biology 2021Quote: ... Myoblasts derived from an adult DM1 patient biopsy (DM-05 (Xia et al., 2013)) were cultured in SkGM-2 skeletal muscle cell growth medium (Lonza, #CC-3245).
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bioRxiv - Molecular Biology 2021Quote: ... 10 μg of pX330 neomycin-resistant vector containing each gRNA and 10 mM ssODN were co-transfected into 2 × 106 ESCs using a Nucleofector (Lonza, program A023). Cells were plated onto two 10 cm dishes containing hygromycin-resistant DR4 MEFs ...
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bioRxiv - Biophysics 2019Quote: Zebrafish between 9 and 13 dpf were anesthetized with Tricaine-S (MS-222, Pentaire; .008% w/v, buffered to pH 7) and embedded in 2% low melting point agarose (Lonza SeaPlaque, #50100) with .004% w/v Tricaine ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... UCB-MNC were thawed and cultured at 2 million cells/mL in X-VIVO 15 serum-free cell-culture medium (Lonza, Basel, Switzerland) supplemented with 0.5 μg/mL of FMS-like tyrosine kinase-3 and 0.5 μg/mL of stem-cell factor ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HUVEC cells were grown at 37o C in the Endothelial cell basal medium-2 (LONZA #Cat No-CC-3156 and CC-4176) under the atmosphere containing 5% CO2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... were ligated to the target fragments and full-length ligation products were purified via gel electrophoresis (2% MetaPhor agarose, Lonza, Basel, Switzerland). Purity and concentration of extracted fragments were determined using capillary electrophoresis (Fragment Analyser ...
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bioRxiv - Immunology 2023Quote: ... 80 µl of supernatant was removed and replenished with 100 µl IMDM/Glutamax/ 10% FBS containing 2× MycoZap Plus-PR (Lonza #VZA-2021), 100 ng/ml human IL-2 (GoldBio #1110-02-50) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HUVEC cells were grown at 37o C in the Endothelial cell basal medium-2 (LONZA #Cat No-CC-3156 and CC-4176) and were used with or without addition of drugs and LPS (0.5 ug/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The culture dish was coated with 0.2% gelatin and the cells were cultured in endothelial growth medium (EGM)-2 complete media (#CC-3162, Lonza Clonetics, Fisher scientific) with EGM-2 SingleQuots supplement kit (CC-4176 ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... with SmGM until confluency and then in SmBM (SmGM without complement) + 2% (v/v) FBS and antibiotics from the Bulletkit for SmGM (CC-4149, Lonza, Basel, Switzerland). SEP was added once at the beginning of the experiment at 100 µg/mL ...
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bioRxiv - Microbiology 2024Quote: ... Non-adherent cells were centrifuged again and resuspended in 2 mL of bronchial epithelial cell growth medium (BEGM) (Lonza cat# CC-3170) supplemented with Primocin (InvivoGen ...
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bioRxiv - Cancer Biology 2024Quote: Fresh single cell suspension remaining from PDOTS processing (S3) was grown in SmGM-2 Smooth Muscle Cell Growth Medium (Lonza, CC-3181) supplemented with SmGM-2 SingleQuots Supplement Pack (Lonza ...
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bioRxiv - Pathology 2020Quote: ... The purity of the final recombinant proteins was more than 99% with an endotoxin concentration lower than 2 units/mg protein by Limulus Amebocyte Lysate PYROGENT™ 125 Plus (Lonza; Walkersville, MD).
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bioRxiv - Evolutionary Biology 2021Quote: For agarose/stiffness preference assays, experimental substrates contained 75mM sucrose, 1% ethanol and increasing concentrations (1%, 1.5%, and 2%) of agarose (Lonza SeaKem LE Agarose #50001); control substrates were 0.25% agarose and contained 75mM sucrose and 1% ethanol.
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bioRxiv - Cell Biology 2020Quote: ... Conditioned media (containing EVs) was removed and the cells washed x 2 with PBS before trypsinisation using 1 × Trypsin-EDTA (Lonza, UK cat: T3924). Cell counts and viability were checked at the time of EV harvest using the trypan blue exclusion assay (0.4% Trypan blue solution ...
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bioRxiv - Immunology 2020Quote: ... followed by washing in complete media (RPMI-1640 containing 10% FBS, 2 mM glutamine, 100 IU/ml penicillin, and 100 µg/ml streptomycin, Lonza, Walkersville, MD, USA). Isolated BAL cells (approx.1-2 x106 ...
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bioRxiv - Molecular Biology 2023Quote: Human primary pulmonary artery ECs (HPAECs) were obtained from ATCC and grown on gelatin-coated dishes in Endothelial Cell Basal Medium-2 (Lonza, Catalog # CC-3156) supplemented with EGM-2 SingleQuots Supplements (Lonza ...
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bioRxiv - Immunology 2024Quote: ... including the use of 2 mM EDTA in PBS as the wash buffer and red blood cell lysis with ACK lysis buffer (Lonza by Thermo Fisher). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... transfecting ∼5×106 cells in 1x Tb-BSF buffer (Schumann Burkard et al., 2011) using 2 mm cuvettes (BTX) with an Amaxa Nucleofector 2b (Lonza, program X-001).
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bioRxiv - Biophysics 2020Quote: ... hydrocortisone and recombinant human epidermal growth factor (MEGM Bullet Kit CC-4136; Lonza). Cells were maintained in an incubator under standard conditions (36.5 °C ...
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bioRxiv - Microbiology 2020Quote: ... and routinely tested for mycoplasma contamination with the MycoAlert Mycoplasma Detection kit (Lonza). Two days before infection ...
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bioRxiv - Cell Biology 2020Quote: Cells were negative for mycoplasma as verified with Mycoalert mycoplasma detection kit (Lonza).
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bioRxiv - Cell Biology 2019Quote: ... PC12 transfections were carried out with Amaxa Nucleofection Kit V (Lonza VCA-1003) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... composed of bronchial epithelial basal medium (BEBM) supplemented with the SingleQuots kit (Lonza) and incubated at 37°C in 5% de CO2 ...
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bioRxiv - Developmental Biology 2020Quote: ... Cell lines were routinely checked for Mycoplasma contaminations every month (LONZA – MYCOALERT KIT), and all samples analyzed in this study were not contaminated.
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bioRxiv - Molecular Biology 2021Quote: ... with a SF Cell Line 4D-Nucleofector X Kit L (Lonza, V4XC-2024) and the EW-113 transfection programme ...
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bioRxiv - Cell Biology 2022Quote: ... some cells were transfected by electroporation using Basic Primary Neurons Nucleofector Kit (Lonza), according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2019Quote: ... Cells were confirmed to be mycoplasma negative via MycoAlertTM Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cancer Biology 2019Quote: ... Cell lines were confirmed mycoplasma negative using Mycoplasma detection kit (Lonza, LT07-418).
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bioRxiv - Genetics 2020Quote: ... including different nucleofection buffers (Human T cell Nucleofector kit, Lonza; 1M nucleofection solution67), nucleofection programs (U014 ...
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bioRxiv - Developmental Biology 2020Quote: ... vector using using the P3 Primary Cell 4D-NucleofectorTM Kit (V4XP-3012 Lonza) and the 4D-Nucleofector™ Platform (Lonza) ...
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bioRxiv - Cell Biology 2020Quote: ... were purchased and verified from Lonza and grown in EMB media supplemented with EGM-SingleQuot Kits (Lonza; Walkersville, MD). Primary human aortic endothelial cells (HAECs ...
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bioRxiv - Cell Biology 2019Quote: ... All cultures were routinely tested for mycoplasma using a MycoAlert PLUS Kit (Lonza) and a 384-well Varioskan LUX (Thermo Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were tested for mycoplasma contamination using the MycoAlert mycoplasma detection kit (Lonza). When treating with rituximab alone or in combination ...
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bioRxiv - Cancer Biology 2019Quote: ... Cultured cells were regularly tested for mycoplasma using the mycoAlert Detection Kit (Lonza).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Mycoplasma testing was performed regularly on cell lines using the MycoAlertTM kit (Lonza); cell lines were mycoplasma-free before experiments were conducted.
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bioRxiv - Cancer Biology 2020Quote: ... Absence of mycoplasma contamination was verified with MycoAlert™ Mycoplasma Detection Kit (Lonza). All methods were carried out in accordance with the approved guidelines of our institution.
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bioRxiv - Bioengineering 2021Quote: CHRF cells were electroporated following manufacturer protocols for the nucleofector kit V (Lonza) with the pLifeAct-miRFP703 plasmid (Addgene #79993) ...
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bioRxiv - Bioengineering 2020Quote: ... All cell lines were confirmed to be mycoplasma negative (MycoAlert Detection Kit, Lonza). Pitstop (ab120687 ...
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bioRxiv - Biophysics 2021Quote: ... “MARCKS”, (Gadi et al., 2011)) via electroporation using Amaxa Nucleofactor kit T (Lonza) as described in (Weatherly et al. ...
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bioRxiv - Biophysics 2021Quote: ... Cells were regularly tested for mycoplasma contamination using MycoAlert Mycoplasma detection kit (Lonza).