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Citations for Lonza :
1251 - 1300 of 4136 citations for Rat Neural Stem Cell Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: CRISPR plasmid constructs (2 μg) were electroporated into 2 × 106 Raji cells using the Amaxa Cell Line Nucleofector Kit V (Lonza Bioscience) (Program M-013) ...
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bioRxiv - Cell Biology 2019Quote: ... HUVEC (primary Human Umbilical Vein Endothelial Cells, CC2517, LOT 0000482213) were maintained in Endothelial Cell Growth Medium Lonza (Lonza, CC-3121) with supplements and growth factors (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... pcDNA3-JUP or empty vector were transfected into Caco-2 cells using SE Cell Line 4D-Nucleofector™ X Kit (Lonza) and an Amaxa 4D Nucleofector device from Lonza ...
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bioRxiv - Bioengineering 2021Quote: ... we nucleofected HEK293FT-RfxCas13d cells with 225 μmol of synthetic crRNAs in 20μl reaction of SF Cell Line Nucleofector Solution (Lonza V4XC-2032) using the Lonza Nucleofector 4D (program CM-130) ...
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bioRxiv - Cell Biology 2021Quote: ... Human primary hepatic stellate cells from donors 3 and 4 were purchased as isolated hepatic stellate cells from Lonza (cat# HUCLS). Donor information is listed below.
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bioRxiv - Microbiology 2021Quote: ... Vero cell cultures were seeded at 1-3 × 104 cells/cm2 in Dulbecco’s minimal essential medium (DMEM, LONZA, Alpharetta, GA, USA) supplemented with 9% foetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... CHO-S cells were resuspended at a density of 4 mio cells/mL in ProCHO 4 medium (Lonza, #LZ-BE12-029Q) supplemented with 8 mM ultraglutamine (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... HPMEC were cultured in endothelial cell growth basal medium 2 supplemented with an Endothelial Cell Growth Medium-2 (EGM-2™) supplemental bullet kit (Lonza). Cells were maintained at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... DNA electroporation was conducted following the Amanxa® Cell line nucleofector protocol using the Cell Line Nucleofector® Kit R (Lonza). 2 μg plasmid DNA was used per reaction and electroporation was carried out using the I-013 program for high expression efficiency of HeLa cells ...
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bioRxiv - Cancer Biology 2021Quote: ... and immortalized human Brain Microvascular Endothelial cells (HBMEC) were seeded at 5000 cells/cm2 in EBM supplemented with 5% FBS and EGM supplements (all products, Lonza Bioscience) until reaching confluence 42,43 ...
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bioRxiv - Molecular Biology 2020Quote: ... PrEC cells (Prostate Epithelial Cells) were cultured in PrEBM basal medium supplemented with PrEGM SingleQuots™ Supplements and Growth Factors (Lonza) and passaged when 80-90% confluent ...
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bioRxiv - Immunology 2022Quote: ... were delivered into the CD4+ Human T cells by using Amaxa 4D-Nucleofector System and P2 Primary Cell 4D-Nucleofector® X Kit S (Lonza) according to manufacturer’s instructions using the pulse code EH100 ...
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bioRxiv - Developmental Biology 2019Quote: ... E14 cells (5 × 106) were transfected with 10 μg of the appropriate plasmid with the Mouse ES Cell Nucleofector™ Kit (Lonza) and allowed to grow for 48h after transfection ...
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bioRxiv - Immunology 2019Quote: ... Leishmania tarentolae cells were transfected via electroporation (Nucleofector™ 2b Device, AmaxaTM Human T Cell Nucleofector™ Kit, Lonza, Basel, Switzerland) with the respective expression cassette containing the selective antibiotic marker Nourseothricin (NTC ...
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bioRxiv - Immunology 2020Quote: ... while lung microvascular cells were maintained on uncoated plates in Microvascular Endothelial Cell Growth Medium-2 (EGM-2 MV; Lonza, UK). Nasal epithelial cells were maintained in Airway Epithelial Growth Media (Promocell ...
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bioRxiv - Immunology 2020Quote: ... Aortic and blood outgrowth endothelial cells were maintained on uncoated plates in Endothelial Cell Growth Medium-2 (EGM-2; Lonza, UK), while lung microvascular cells were maintained on uncoated plates in Microvascular Endothelial Cell Growth Medium-2 (EGM-2 MV ...
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bioRxiv - Genomics 2021Quote: ... K562 cells were grown to a density of 1×106 cells and electroporated with 5μg of plasmid DNA (Amaxa® Cell Line Nucleofector® Kit V, Lonza). Cells from each cell line were cultured for an additional 48 hours until total RNA isolation (Maxwell RSC simplyRNA kit ...
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bioRxiv - Immunology 2022Quote: ... In vitro transcribed mRNAs encoding the TCR α- and β-chains were mixed at a 1:1 ratio and electroporated in activated T cells on a Lonza 4D-NucleofectorTM device using the P3 Primary Cell 4D-Nucleofector™ X Kit (Lonza) according to the recommendations of the manufacturer ...
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bioRxiv - Bioengineering 2023Quote: ... were delivered into cells at a ratio of 2.5:1 by Lipofectamine transfection in HEK 293T cells or by electroporation in Raw 264.7 cells using the Lonza 4D-Nucleofector and the SF kit (Lonza, V4XC-2032). Puromycin selection (5 μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... were assembled in vitro following the manufacturer’s protocol and delivered into OCI-Ly7 cells by electroporation using SF Cell Line 96-well Nucleofector Kit (Lonza; V4SC-2096). ssODN for CREBBP R1446C mutagenesis was added along with RNP complex during electroporation as needed ...
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bioRxiv - Cancer Biology 2023Quote: ... Co-expression plasmids were delivered by nucleofection into U2OS DSB reporter cell lines using the SE Cell Line 4D-Nucleofector XL Kit (Lonza Biosciences). To induce a DSB ...
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bioRxiv - Microbiology 2023Quote: ... 2 ×□106 unstimulated CD4+ T cells or 0.2 × 106 CD34+ cells were washed with PBS and resuspended in 20□μl buffer P3 (Lonza #V4XP-3032). To deliver two specific sgRNAs targeting one gene ...
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bioRxiv - Physiology 2024Quote: The gRNA plasmid (1µg) and donor template plasmid (3µg) were introduced into 3T3-L1 cells (1X 10 6 cell) by electroporation (LONZA, Basel, Switzerland). After 48h of electroporation ...
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bioRxiv - Cancer Biology 2023Quote: ... K562 and NALM6 cells (2x105 cells/transfection) were transfected using a Lonza 4D nucleofector™ with a SF nucleofection kit (Lonza) following manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary CD3+ T cells (1x106 cells/transfection) were transfected using a Lonza 4D nucleofector™ (Pulse code EH115) with a P3 nucleofection kit (Lonza) following manufacturer’s recommendations with the indicated DNA concentrations ...
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bioRxiv - Immunology 2021Quote: ... 1.5×106 cells multiplied by the number of mice to be injected were transfected with the pIL-10 vector (1×106 cells/1.5 μg pIL-10 DNA per cuvette) using the Mouse T Cell Nucleofector Kit (Lonza, No: VPA-1006) with Nucleofector II Device (program X-100) ...
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bioRxiv - Cell Biology 2020Quote: ... 1×106 cells (grown in 6-well dishes) were subjected to nucleofection with Amaxa® Cell Line Nucleofector® kit V (VCA-1003, Lonza), following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... MDA-MB-231 bulk or stable populations overexpressing Nluc-CD63 or Nluc-CD9 constructs were obtained using an electroporation-based transfection protocol optimized for this cell line (Amaxa® Cell Line Nucleofector® Kit V, Lonza). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HUVEC cells were grown at 37° C in the Endothelial cell basal medium-2 (LONZA #Cat No-CC-3156 and CC-4176) under the atmosphere containing 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... subjected to red blood cell lysis with ACK lysis buffer and electroporated with P3 Primary Cell 4D-NucleaofactorTM X -kit (V4XP-3032, Lonza, Basel, Switzerland), as previously described (Freund et al. ...
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bioRxiv - Cell Biology 2021Quote: ... We transfected the KCNQ1 constructs and the empty vector into our established mouse pancreatic β cells (referred to as SJ β cells (Jia et al., 2015)) using AmaxaTM Cell Line NucleofectorTM Kit V and AmaxaTM NucleofectorTM 2b device (Lonza, Switzerland). The glucose-sensitive mouse β-cell line SJ-β was cultured and an insulin-secretion assay performed as reported earlier (Jia et al. ...
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bioRxiv - Cell Biology 2019Quote: Human umbilical vein endothelial cells (HUVECs) and the hCMEC/d3 human blood-brain barrier cell line were obtained from Lonza (Walkersville, MD) and Sigma (St ...
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bioRxiv - Cancer Biology 2021Quote: ... Immortalized human bronchial epithelial BEAS-2B cells were purchased from ATCC and cultured in BEGM bronchial epithelial cell growth medium (Lonza #CC-4175). All the cells were grown at 37°C with 5% CO2.
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bioRxiv - Evolutionary Biology 2022Quote: ... and used to infect fresh Sf9 cells (1.2 × 106 cells* ml) in 10 ml of Insect-Xpress medium (Lonza, Walkersville, MD, USA) with 15 mg/ml gentamycin (Roth ...
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bioRxiv - Microbiology 2022Quote: ... 1.2E6 BHK21 cells were nucleofected with 2 µg of Spike plasmid using an Amaxa Nucleofector II with cell line kit L (Lonza #VCA-1005) and program A-031 ...
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bioRxiv - Cell Biology 2022Quote: ... from Lonza were cultured in T-75 cell culture flasks in Endothelial Cell Growth Medium-2 (EGM-2) (BulletKit, Lonza, Cat. No. CC-3162). EGM2 was made from Endothelial cell Basal Medium – 2 (EBM2) ...
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bioRxiv - Cell Biology 2022Quote: ... 1uM siRNA was electroporated into cells using the Amaxa Nucleofector II device and Cell Line Nucleofector Kit V (Lonza Bioscience, VCA-1003). B16-F10 cells were electroporated with program P-020 ...
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bioRxiv - Microbiology 2021Quote: ... the Sleeping Beauty transposase system [37] was adapted to electroporate Jurkat cells using the Lonza SE Cell Line kit (Lonza, V4SC-1960). The pSBbi-RP plasmid was a gift from Eric Kowarz (Addgene plasmid #60513 ...
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bioRxiv - Immunology 2019Quote: ... conjugated to FITC or GFP siRNA (300 nM) conjugated to FITC two days prior to adoptive T cell therapy using the Mouse T cell Nucleofector™ Kit (Lonza Bioscience) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... as a control two days prior to adoptive T cell therapy using the P3 Primary Cell 4D-Nucleofector X™ Kit (Lonza Bioscience) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... and the GFP-K17ΔNLS (Hobbs et al., 2015) were nucleofected into HeLa cells using SE Cell Line 4D X nucleofector Kit S (Lonza #V4XC-1032) with setting DS-138 ...
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bioRxiv - Immunology 2021Quote: ... gRNA_pX335 plasmids were transfected into EL4 cells by nucleofection using an Amaxa nucleofector and the Cell Line Nucleofector Kit L (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... UCB-MNC were thawed and cultured at 2 million cells/mL in X-VIVO 15 serum-free cell-culture medium (Lonza, Basel, Switzerland) supplemented with 0.5 μg/mL of FMS-like tyrosine kinase-3 and 0.5 μg/mL of stem-cell factor ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HUVEC cells were grown at 37o C in the Endothelial cell basal medium-2 (LONZA #Cat No-CC-3156 and CC-4176) under the atmosphere containing 5% CO2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... human aortic endothelial cells (CD31+) from were maintained in EGM™-2 Endothelial Cell Growth Medium-2 Bullet Kit™ (Cat # CC-3162; Lonza) and EGM™ −2 MV Microvascular Endothelial Cell Growth Medium-2 Bullet KitTM (Cat #CC-3202 ...
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bioRxiv - Immunology 2022Quote: ... puromycin containing medium were changed and every 4 days cells were fed EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ (LONZA) supplemented with GlutaMax (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... and BEBM™ Bronchial Epithelial Cell Growth Basal Medium supplemented with the BEGM™ Bronchial Epithelial Cell Growth Medium Single Quots™ Supplements and Growth Factors (Lonza). Fresh media were added to the cells every 2-3 days until the confluent monolayer of mesothelial cells was obtained (typically between 5-10 days post-isolation).
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bioRxiv - Neuroscience 2023Quote: ... 8 x 105 hiPSCs in single-cell suspension were nucleofected with 5 μg SpCas9-sgRNA plasmid using the P3 Primary Cell 4D-Nucleofector Kit (Lonza, #V4XP-3024) in combination with the 4D Nucleofector Unit X (Lonza ...
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bioRxiv - Developmental Biology 2023Quote: ... ePB master vector and helper (Plasmid AW-27) were nucleofected into the established SOX2::mCitrine cell line using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza; V4Xp-3012). G-418 (40ng/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... MV411 cells were electroporated with Cas9/sgRNA complexes targeting the HDR insertion using a Lonza SF Cell Line 4D Nucleofector (Lonza V4XC-2032). RNP complexes were formed by mixing 8.5 μg of TrueCut Cas9 Protein v2 (Invitrogen A36499 ...