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Citations for Lonza :
1151 - 1200 of 1688 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... electroporation was carried out following the instructions of the Mouse Embryonic Stem Cell Nucleofector Kit from Lonza. After 2 days recovery ...
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bioRxiv - Cancer Biology 2022Quote: ... UWB1 and UWB1+BRCA1 cells were maintained in RPMI1640 (ATCC) and MEGM bullet kit (1:1; Lonza) with 3% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... 5 × 105 cells were nucleofected (Amaxa® HUVEC Nucleofector® Program A-034, Kit #VAPB-1002, Lonza) with 50 nM of the following siRNA oligos ...
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bioRxiv - Molecular Biology 2022Quote: ... and re-suspended in 20 μL NF-solution (P3 Primary Cell 4D-Nucleofector™ X Kit, Lonza). Thereafter ...
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bioRxiv - Neuroscience 2022Quote: ... Passages under 50 were used and cultures were regularly tested for mycoplasma using a MycoAlert Kit (Lonza). Human iMGLs were differentiated as previously described(26) ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% FBS (Hyclone) and Microvascular Endothelial Growth Medium growth factor kits (Lonza Cat# CC-4147). HLMVECs between passages 5 and 9 were used for experiments ...
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bioRxiv - Microbiology 2022Quote: ... Cultures were kept mycoplasma free and mycoplasma contaminations were monitored weekly using the Mycoalert Luminescence Kit (Lonza).
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bioRxiv - Molecular Biology 2023Quote: A Lonza Nucleofector system including a Nucleofector 2b Device and the Cell Line Nucleofector Kit V (Lonza) were used for electroporation of LbuCas13a protein and guide RNA ...
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bioRxiv - Microbiology 2023Quote: siRNAs targeting bovine hnRNPM were introduced into BL3.1 cells using Amaxa cell line Nucleofector kit V (Lonza) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... following the protocol of 4D-Nucleofector™ Solution (SE Cell Line 4D-Nucleofector™ X Kit, Lonza). In this setup ...
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bioRxiv - Cell Biology 2023Quote: ... Electroporation of the RNP complex was carried out using the SF Cell Line 4D-Nucleofector kit (Lonza) and program CM-130 with 2.0×105 cells ...
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bioRxiv - Bioengineering 2023Quote: ... 1×106 cells were nucleofected using the Lonza SF Cell Line 4D-Nucleofector Kit (Lonza V4XC-2012) and a Lonza 4D Nucleofector (Lonza ...
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bioRxiv - Cancer Biology 2023Quote: ... using the P3 Primary Cell 4D-Nucleofector X Kit for Amaxa 4-D device (Lonza, #V4XP-3032). 2×10E5 cells per condition were nucleofected in separated strip wells using program EO-100 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 million cells were resuspended in 20 uL of the Amaxa NHDF Nucleofector Kit (Lonza, Walkersville, MD), containing 1ug of each of the gRNA and donor plasmid vectors (ABM ...
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bioRxiv - Cell Biology 2023Quote: ... and these cells were transferred to a cuvette (P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza) for nucleofection in a 4D-Nucleofector (Lonza ...
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bioRxiv - Molecular Biology 2023Quote: ... using the CRISPR/Cas9 system and the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza, Switzerland). Oligonucleotides for gRNA were selected by Сrispor (Tefor Infrastructure ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were confirmed negative for mycoplasma contamination using the MycoAlert PLUS kit (Lonza, #LT07–710). Non-targeting control (NT CT) ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured in Endothelial Basal Media-Plus supplemented with SingleQuots Bullet Kit (EGM-Plus, CC-5035 Lonza) until passage 2-3 for 3D cultures and passage 3-7 for 2D cultures ...
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bioRxiv - Cell Biology 2023Quote: ... 1 million cells were resuspended in 100 mL of the AmaxaTM NHDF Nucleofector kit (Lonza, Walkersville, MD), containing 1ug of each of the four episomal plasmid vectors encoding OCT3/4 and p53 shRNA ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... cells were transfected using Nucleofection with an Amaxa SF Cell Line 4D-Nucleofector X kit S (Lonza) and program FF-113 (HT1080 ...
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bioRxiv - Biophysics 2023Quote: ... Cells were transfected by nucleofection using the Lonza Cell line Nucleofector Kit V (Lonza, Basel, Switzerland, #VVCA1003) and the Amaxa Nucleofector II device ...
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bioRxiv - Bioengineering 2023Quote: Nucleofection in T cells were performed using P3 Primary Cell 4D-Nucleofector™ X Kit S (Lonza) according to the manufacturer’s instruction with minor modifications ...
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bioRxiv - Neuroscience 2024Quote: ... and the nucleofection reaction was prepared using the Cell Line Nucleofector™ Kit V (Lonza; VCA-1003) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Nucleofection was performed using the P3 Primary Cell 4D-Nucleofector™ X Kit S (Lonza, V4XP-3032), a 4D-nucleofector core unit ...
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bioRxiv - Molecular Biology 2021Quote: ... and transfected using the Lonza Amaxa® Cell Line Nucleofector® Kit V (Lonza, Basel, Switzerland VCA-1003) using electroporation program A30 ...
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bioRxiv - Immunology 2022Quote: ... 2×106 B cells were then nucleofected with 2 μg of plasmid DNA using Nucleofector Kit V (Lonza) and rested for at least 16-24 hours using complete media containing 5 ng/ml BAFF and lacking LPS ...
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bioRxiv - Molecular Biology 2020Quote: ... This vector and the piggy bac transposase were then nucleofected into H9 using the AMAXA nucleofector kit (Lonza). Puromycin selection started 4 days following nucleofection and the surviving clones were screened by qPCR and Western Blot for NSUN6 expression ...
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bioRxiv - Cancer Biology 2019Quote: ... All lines were validated by STR analysis yearly and checked for mycoplasma contamination using MycoAlert PLUS kit (Lonza). Conditioned medium was collected by starving sub-confluent cells for 24 h in α-MEM (Gibco).
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bioRxiv - Microbiology 2019Quote: ... All POLIC variant expression constructs (15 µg) were transfected by nucleofection using the Amaxa Nucleofection Parasite Kit (Lonza) into 29-13 cells to generate inducible overexpression POLIC-PTP variant cell lines (OE ...
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bioRxiv - Immunology 2019Quote: ... gRNA and Cas9 containing plasmids were introduced to prostate epithelial cells using the basic nucleofector kit (Amaxa, Lonza) following the manufacturer’s instructions for primary mammalian epithelial cells (program W001) ...
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bioRxiv - Immunology 2021Quote: ... The P14 cell/Cas9/RNP mixes were transferred to Nucleofection cuvette strips (4D-Nucleofector X kit S; Lonza). Cells were electroporated using a 4D nucleofector (4D-Nucleofector Core Unit ...
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bioRxiv - Cancer Biology 2020Quote: ... The presence of mycoplasma was tested for frequently in all cell lines with a MycoAlert kit (Lonza, Switzerland), exclusively using mycoplasma-free cells in all the experiments carried out.
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... PDAC cells grown as 3D organoids were regularly tested for mycoplasma contamination using the MycoAlert Plus kit (Lonza) or the Mycoprobe Mycoplasma Detection Kit (R&D Systems).
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bioRxiv - Neuroscience 2021Quote: ... counted and 2×106 cells were transfected using the Basic Nucleofector kit for primary neurons (VAPI-1003, Lonza) and the D-33 programme on the Amaxa Nucleofector II B device (Amaxa Biosystems) ...
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bioRxiv - Neuroscience 2022Quote: ... 5 × 106 trypsinized cells were resuspended in 93.5 µl solution (mouse ES cell nucleofector kit, VPH-1001, Lonza). The solution includes 2 µg of each CRISPR/Cas9 vector (4 µg total ...
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bioRxiv - Neuroscience 2022Quote: ... 20,000 cells were resuspended in 100 μL nucleofection buffer from Human Stem Cell Nucleofector™ Kit 2 (Lonza). Salsa6f-AAVS1 SHL plasmid Template (2 μg ...
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bioRxiv - Bioengineering 2022Quote: ... The iECs were cultured in endothelial cell growth medium 2 kit supplemented into basal media (except hydrocortisone, Lonza) with 1x GlutaMax (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... 1×106 cells per guide were electroporated with the corresponding RNP complex using Lonza Electroporation Kit V (Lonza). After 48 h ...
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bioRxiv - Neuroscience 2021Quote: ... coated coverslips in a 24-well plate in 1ml Endothelial Growth Media-2 Bullet Kit (EGM-2; Lonza; Endothelial basal media-2 with 2% Fetal Bovine Serum (FBS) ...
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bioRxiv - Microbiology 2020Quote: Electroporation was performed using the Amaxa P3 Primary Cell 96-well Nucleofector kit and 4D Nucleofector system (Lonza). Recombinant S ...
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bioRxiv - Neuroscience 2021Quote: ... freshly isolated cortical neurons were transfected using the P3 Primary Cell 4D-Nucleofector Kit (Lonza, LZ-V4XP-3024) and the program DC-104 of the 4D-Nucleofector device (Lonza) ...
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bioRxiv - Cell Biology 2021Quote: ... RNP was delivered by electroporation using a Lonza 4D-nucleofector and P3 Primary Cell Kit (Lonza, V4XP-3012). Cells were transferred to fresh StemSpan media and corresponding (AK2 or AAVS1 ...
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bioRxiv - Cancer Biology 2021Quote: ... A human breast MCF10A cell line (CRL10317, ATCC) was grown with completed growth medium: MEGM Kit (Lonza CC3150) without gentamycin-amphotericin B mix (GA1000 ...
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bioRxiv - Genetics 2022Quote: ... were cultured in RtEGM with supplement medium as indicated by the manufacturer’s protocol (RtEGM bullet kit, Lonza, #00195409). HfRPE cells were cultured to high confluence on coverglass culture plates (Thermo ...
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bioRxiv - Immunology 2022Quote: ... 1 x 10^6 purified naive T-cells were resuspended in 15µL buffer P3 + 5µL Supplement 1 (P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza) and mixed with 2.7µL RNP in the 16-well strips provided in the kit ...
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bioRxiv - Cell Biology 2019Quote: Stably transfected U2OS cells were generated using using Cell Line Nucleofector Kit V and a Nucleofector electroporator (Lonza). After 24 hours ...
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bioRxiv - Pathology 2020Quote: ... Plasmid delivery through electroporation of passage three neurospheres was performed using Mouse neural stem cell nucleofector kit (Lonza).
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bioRxiv - Cancer Biology 2020Quote: ... H2228 cells were electroporated with 3ug DRGFP substrate using reagent T (Amaxa Cell Line Nucleofector Kit T, Lonza), program X-001 on the Nucleofector 2b (Lonza) ...
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bioRxiv - Microbiology 2020Quote: ... 1 million Jurkat T-cells were resuspended in 100 μL Nucleofector solution (Cell Line Nucleofector™ Kits, Lonza) and combined with 2 μg of the linearized dual-fluorophore vector and 2 μg of the corresponding gRNA/Cas9 plasmid ...
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bioRxiv - Genomics 2021Quote: ... K562 cells were grown to 1×106 and transfected with 1,000ng of gRNA plasmid and 3,000 ng of pCMV_AncBE4max_P2A_GFP (Amaxa® Cell Line Nucleofector® Kit V, Lonza). K562 cells were cultured for an additional 48 hours then single clones of green cells were isolated using flow cytometry (Aria II) ...